Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,867,626 A→G K34K (AAA→AAG)  FTN78_chr1914 → Site‑specific recombinase, DNA invertase Pin related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,867,6260AG99.8% 1503.2 / NA 422K34K (AAA→AAG) FTN78_chr1914Site‑specific recombinase, DNA invertase Pin related protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (208/213);  total (208/214)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 437 total aligned reads displayed.

GAAAGAACGGCTCGTAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAG  >  CP043523/1867481‑1867772
                                                                                                                                                 |                                                                                                                                                  
gataacatcactcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTa                                                                                                                                                <  1:2336468‑M1/135‑1 (MQ=255)
 ataacatcactcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTAc                                                                                                                                               <  2:3346449‑M1/136‑1 (MQ=255)
   aacatcactcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTtactg                                                                                                                                             <  2:4092228‑M1/138‑3 (MQ=255)
      atcactcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAgtttactggaa                                                                                                                                          >  2:2184525‑M1/10‑145 (MQ=255)
       tcactcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAagtttactggaac                                                                                                                                         >  2:4013479‑M1/9‑144 (MQ=255)
         actcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTa                                                                                                                                       >  2:877909‑M1/7‑150 (MQ=255)
         actcacAACTTAGGGTTATGAATAGAGGCCAAAATATGAAAACTGGGTAGGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGACGTTTTCCAAGAAAAGTTTACCGGAACTa                                                                                                                                       <  1:1744795‑M1/144‑1 (MQ=255)
          ctcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTAc                                                                                                                                      >  1:3035342‑M1/6‑150 (MQ=255)
           tcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACa                                                                                                                                     >  1:3533666‑M1/5‑150 (MQ=255)
            cacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCTAAGAAAAGTTTACTGGAACTACAg                                                                                                                                    <  1:3984456‑M1/147‑1 (MQ=255)
             acAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAAGAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGt                                                                                                                                   >  2:4301474‑M1/3‑150 (MQ=255)
             acAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGt                                                                                                                                   >  2:2160304‑M1/3‑150 (MQ=255)
             acAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGt                                                                                                                                   >  1:108430‑M1/3‑150 (MQ=255)
               aaCTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGtcg                                                                                                                                 >  1:3908393/1‑148 (MQ=255)
                  ttAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAAc                                                                                                                              <  1:1894166/150‑1 (MQ=255)
                  ttAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAAc                                                                                                                              <  1:4543926/150‑1 (MQ=255)
                      ggTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTcc                                                                                                                          >  2:125481/1‑150 (MQ=255)
                         tattaATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGATAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCcaca                                                                                                                       >  1:3998206/1‑150 (MQ=255)
                           ttaATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaat                                                                                                                     <  2:1895119/150‑1 (MQ=255)
                           ttaATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaat                                                                                                                     >  1:3641522/1‑150 (MQ=255)
                            taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                    <  2:599003/150‑1 (MQ=255)
                             aaTAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaattt                                                                                                                   >  1:1018003/1‑150 (MQ=255)
                             aaTAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaattt                                                                                                                   <  2:3778121/150‑1 (MQ=255)
                                agagGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatttaat                                                                                                                >  2:1437319/1‑150 (MQ=255)
                                agagGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatttaat                                                                                                                <  1:428784/150‑1 (MQ=255)
                                agagGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatttaat                                                                                                                <  1:1236194/150‑1 (MQ=255)
                                agagGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatttaat                                                                                                                <  1:2412168/150‑1 (MQ=255)
                                  agGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatttaatct                                                                                                              <  2:246709/150‑3 (MQ=255)
                                    gTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAg                                                                                                            <  2:3438351/150‑1 (MQ=255)
                                    gTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAg                                                                                                            <  1:676601/150‑1 (MQ=255)
                                     tCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGt                                                                                                           <  1:1125431/150‑1 (MQ=255)
                                      cAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGta                                                                                                          <  1:758398/150‑2 (MQ=255)
                                       aaaaTATGAAAATTGGGTATGCACGTGTCAGTGCTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGATTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtat                                                                                                         >  2:4362254/1‑148 (MQ=255)
                                        aaaTATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtatt                                                                                                        >  2:3274461/1‑147 (MQ=255)
                                         aaTATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtattt                                                                                                       <  2:4141533/150‑5 (MQ=255)
                                            atGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtatttaaa                                                                                                    <  1:4591075/150‑8 (MQ=255)
                                                ctgttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCATATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTAGTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGt                                                                                                >  2:1829457/4‑150 (MQ=255)
                                                 aaTTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGtt                                                                                               <  1:4340695/150‑1 (MQ=255)
                                                 aaTTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATAAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGtt                                                                                               >  2:4517411/1‑150 (MQ=255)
                                                   ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGCAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATTTAGTATTTAAAAAGTTaa                                                                                             <  2:2809675/150‑1 (MQ=255)
                                                   ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaa                                                                                             <  1:4116172/150‑1 (MQ=255)
                                                    tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                            <  1:95738/150‑2 (MQ=255)
                                                         aTGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggatg                                                                                       >  2:1096112/1‑144 (MQ=255)
                                                                    aatACTTTTGAACTAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTa                                                                            <  1:2621278/148‑1 (MQ=255)
                                                                       aCTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAAtt                                                                         <  1:741879/150‑1 (MQ=255)
                                                                        cTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                        >  1:2913650/1‑150 (MQ=255)
                                                                          tttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgtg                                                                      <  2:210608/150‑1 (MQ=255)
                                                                           ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGa                                                                     >  2:3491824/1‑150 (MQ=255)
                                                                           ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGa                                                                     >  2:2197499/1‑150 (MQ=255)
                                                                            tGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGAc                                                                    >  2:2614355/1‑150 (MQ=255)
                                                                             gAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACa                                                                   >  1:1580679/1‑150 (MQ=255)
                                                                             gAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTCAAAAAGTTAAAGGATGGTGACACTCTAACTGTGACa                                                                   >  2:1340637/1‑150 (MQ=255)
                                                                               aCAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaaa                                                                 >  2:1683675/1‑150 (MQ=255)
                                                                               aCAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaaa                                                                 >  1:228019/1‑150 (MQ=255)
                                                                                caaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAg                                                                >  1:283935/4‑150 (MQ=255)
                                                                                  aaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGtt                                                              >  2:786730/2‑150 (MQ=255)
                                                                                    aaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAg                                                            >  1:1302550/1‑150 (MQ=255)
                                                                                     aTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGa                                                           >  2:2439307/1‑150 (MQ=255)
                                                                                      ttAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGac                                                          <  2:459613/150‑2 (MQ=255)
                                                                                      ttAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGac                                                          <  2:951798/150‑2 (MQ=255)
                                                                                        aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCg                                                        <  1:2153985/150‑1 (MQ=255)
                                                                                         gAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGa                                                       <  1:3012548/150‑1 (MQ=255)
                                                                                          aaTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAt                                                      >  1:3115261/1‑150 (MQ=255)
                                                                                            tctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTa                                                    <  1:3771663/150‑1 (MQ=255)
                                                                                               cAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGcc                                                 <  2:2403825/150‑1 (MQ=255)
                                                                                                   ttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGa                                             <  2:3019804/150‑1 (MQ=255)
                                                                                                   ttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGa                                             <  1:3743522/150‑1 (MQ=255)
                                                                                                    tGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGaa                                            >  2:3117332/1‑150 (MQ=255)
                                                                                                     gAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAac                                           >  1:4437489/1‑150 (MQ=255)
                                                                                                      aaGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAaca                                          <  2:1957415/150‑1 (MQ=255)
                                                                                                      aaGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAaca                                          <  1:4172599/150‑1 (MQ=255)
                                                                                                           cTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                     >  1:1855793/1‑148 (MQ=255)
                                                                                                           cTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTAGTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                     <  1:2096898/150‑3 (MQ=255)
                                                                                                            tGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTagag                                    >  1:651633/1‑147 (MQ=255)
                                                                                                             gAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgaga                                   <  1:16940/150‑1 (MQ=255)
                                                                                                             gAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgaga                                   >  1:2563411/1‑150 (MQ=255)
                                                                                                              aaaGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgagaa                                  <  1:2481370/150‑1 (MQ=255)
                                                                                                               aagaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgagaag                                 >  2:719345/1‑150 (MQ=255)
                                                                                                                agaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGt                                >  2:1879359/1‑150 (MQ=255)
                                                                                                                  aagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGttt                              >  1:1997384/1‑150 (MQ=255)
                                                                                                                  aagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGttt                              <  2:2747617/150‑1 (MQ=255)
                                                                                                                    gCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTa                            <  1:577042/150‑1 (MQ=255)
                                                                                                                     cAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAg                           >  1:1491807/1‑150 (MQ=255)
                                                                                                                        gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                        <  1:335371/150‑1 (MQ=255)
                                                                                                                        gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                        >  1:3440036/1‑150 (MQ=255)
                                                                                                                         aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAt                       <  1:2189582/150‑1 (MQ=255)
                                                                                                                         aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAt                       >  1:2010274/1‑150 (MQ=255)
                                                                                                                           cAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTg                     >  2:2303986/1‑150 (MQ=255)
                                                                                                                            agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                    >  2:2906369/1‑150 (MQ=255)
                                                                                                                            agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                    >  2:638332/1‑150 (MQ=255)
                                                                                                                            agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                    >  1:3267976/1‑150 (MQ=255)
                                                                                                                             gaagaaGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGAGACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGtn                   <  2:4156189/150‑2 (MQ=255)
                                                                                                                              aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                  >  1:826894/1‑150 (MQ=255)
                                                                                                                               agaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCa                 <  2:3998206/150‑1 (MQ=255)
                                                                                                                                       tCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTAt         >  2:1316043/1‑150 (MQ=255)
                                                                                                                                         cAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTAttt       >  2:1006156/1‑150 (MQ=255)
                                                                                                                                         cAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTAttt       >  1:4123122/1‑150 (MQ=255)
                                                                                                                                          aaGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTa      <  2:1547025/150‑1 (MQ=255)
                                                                                                                                             aaaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa   <  2:3641522/150‑1 (MQ=255)
                                                                                                                                             aaaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa   >  1:2258728/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GAAAGAACGGCTCGTAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAG  >  CP043523/1867481‑1867772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑