Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,867,632 G→T T36T (ACG→ACT)  FTN78_chr1914 → Site‑specific recombinase, DNA invertase Pin related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,867,6320GT99.8% 1367.4 / NA 407T36T (ACG→ACT) FTN78_chr1914Site‑specific recombinase, DNA invertase Pin related protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (198/208);  minor base C (0/1);  total (198/209)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 407 total aligned reads displayed.

GGCTCGTAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAT  >  CP043523/1867489‑1867778
                                                                                                                                               |                                                                                                                                                  
cactcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACt                                                                                                                                              >  1:792501‑M1/8‑150 (MQ=255)
 actcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTa                                                                                                                                             <  1:4469799‑M1/144‑1 (MQ=255)
   tcacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACa                                                                                                                                           <  1:3699760‑M1/146‑1 (MQ=255)
    cacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCTAAGAAAAGTTTACTGGAACTACAg                                                                                                                                          <  1:3985881‑M1/147‑1 (MQ=255)
    cacAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAg                                                                                                                                          >  2:850332‑M1/4‑150 (MQ=255)
     acAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAAGAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGt                                                                                                                                         >  2:4301474‑M1/3‑150 (MQ=255)
     acAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGt                                                                                                                                         >  1:108430‑M1/3‑150 (MQ=255)
     acAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGt                                                                                                                                         >  1:1808410‑M1/3‑150 (MQ=255)
          ttAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAAc                                                                                                                                    <  1:1894166/150‑1 (MQ=255)
          ttAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAAc                                                                                                                                    <  1:666384/150‑1 (MQ=255)
             gggTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTc                                                                                                                                 >  1:2116338/1‑150 (MQ=255)
                ttattaATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCcac                                                                                                                              <  1:2553235/150‑1 (MQ=255)
                   ttaATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCGAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaat                                                                                                                           <  2:4144967/150‑1 (MQ=255)
                   ttaATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaat                                                                                                                           <  2:1891574/150‑1 (MQ=255)
                    taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                          <  1:1703328/150‑1 (MQ=255)
                    taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                          >  2:2350467/1‑150 (MQ=255)
                    taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                          <  1:4551180/150‑1 (MQ=255)
                    taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                          >  2:346444/1‑150 (MQ=255)
                    taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                          >  2:3771663/1‑150 (MQ=255)
                    taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                          <  1:3635442/150‑1 (MQ=255)
                    taATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatt                                                                                                                          <  2:599003/150‑1 (MQ=255)
                     aaTAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaattt                                                                                                                         >  1:1018003/1‑150 (MQ=255)
                        agagGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACaatttaat                                                                                                                      <  1:1236194/150‑1 (MQ=255)
                          agatCAAAATATTAAAATTAGGTATGCACGTATCAATACTTTTAAACAAAAATTAAAATCTCAAATTTAATTTCTGAAAAAATCAAGAACAGAAGAAGTTTTCCAAGAAAAGTTTACTCCAACTACAATCTAACGTCCACaatttaatct                                                                                                                    <  2:301704/147‑3 (MQ=255)
                            gTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAg                                                                                                                  >  1:2412822/1‑150 (MQ=255)
                            gTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCAAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAg                                                                                                                  >  2:2628930/1‑150 (MQ=255)
                             tCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGt                                                                                                                 <  1:1125431/150‑1 (MQ=255)
                              cAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGta                                                                                                                <  2:2447409/150‑2 (MQ=255)
                               aaaaTATGAAAATTGGGTATGCACGTGTCAGTGCTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGATTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtat                                                                                                               >  2:4362254/1‑148 (MQ=255)
                               aaaaTATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtat                                                                                                               >  1:4116922/1‑148 (MQ=255)
                                       aaaaTTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAg                                                                                                       >  2:2760024/1‑150 (MQ=255)
                                           ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaa                                                                                                   >  2:1426319/1‑150 (MQ=255)
                                            tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGTAGTTTTCCAAGAAAAGTTTACTGGAACTACATTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                  <  2:4039757/150‑2 (MQ=255)
                                            tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                  >  1:1547025/1‑149 (MQ=255)
                                            tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                  <  1:304665/150‑2 (MQ=255)
                                            tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                  >  1:2940872/1‑149 (MQ=255)
                                             nggTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaag                                                                                                 >  2:4118523/2‑148 (MQ=255)
                                                  tGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggatgg                                                                                            >  2:2191507/1‑143 (MQ=255)
                                                  tGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggatgg                                                                                            >  2:2161985/1‑143 (MQ=255)
                                                            aatACTTTTGAACTAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTa                                                                                  <  1:2621278/148‑1 (MQ=255)
                                                               aCTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAAtt                                                                               <  1:2846798/150‑1 (MQ=255)
                                                               aCTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTAACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTCAAtt                                                                               >  2:1735493/1‑150 (MQ=255)
                                                                cTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                              >  2:2251642/1‑150 (MQ=255)
                                                                cTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                              >  1:701735/1‑150 (MQ=255)
                                                                 ttttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgt                                                                             >  1:3979612/1‑150 (MQ=255)
                                                                  tttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgtg                                                                            <  2:210608/150‑1 (MQ=255)
                                                                   ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGa                                                                           >  2:3491824/1‑150 (MQ=255)
                                                                    tGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGAc                                                                          >  1:2096007/1‑150 (MQ=255)
                                                                      aaCAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaa                                                                        >  2:2416600/1‑150 (MQ=255)
                                                                        caaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAg                                                                      <  2:1300353/147‑1 (MQ=255)
                                                                         aaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGt                                                                     <  1:4379427/148‑1 (MQ=255)
                                                                           aaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTa                                                                   <  1:4290439/150‑1 (MQ=255)
                                                                            aaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAg                                                                  <  1:2006139/150‑1 (MQ=255)
                                                                            aaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAg                                                                  <  2:1878218/150‑1 (MQ=255)
                                                                             aTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGa                                                                 <  2:2897009/150‑1 (MQ=255)
                                                                              ttAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGac                                                                <  2:459613/150‑2 (MQ=255)
                                                                               tAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGacc                                                               >  2:1184331/1‑148 (MQ=255)
                                                                                aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGaccg                                                              >  1:225221/1‑147 (MQ=255)
                                                                                aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGaccg                                                              >  1:3019804/1‑147 (MQ=255)
                                                                                aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCg                                                              >  2:133845/1‑150 (MQ=255)
                                                                                  aaTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAt                                                            <  2:3273402/150‑1 (MQ=255)
                                                                                   aTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAtt                                                           >  1:1975276/1‑150 (MQ=255)
                                                                                     ctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAg                                                         >  2:1667633/1‑150 (MQ=255)
                                                                                      tcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGc                                                        <  1:989144/150‑1 (MQ=255)
                                                                                         aaTTGAAGTTCTGAAAGAAGCAGGAGAAGAAGAAGTTTTCCAAAAAAAATTTACTGGAACTACAGTCAAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAg                                                     <  2:4479785/150‑1 (MQ=255)
                                                                                          nttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAgg                                                    >  2:947998/2‑150 (MQ=255)
                                                                                           ttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAGGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTGTAGTTGTGACAAAGTTAGACCGATTAGCCAgat                                                   >  2:1915594/1‑148 (MQ=255)
                                                                                            tGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGaa                                                  >  1:1583104/1‑150 (MQ=255)
                                                                                               aGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                               >  2:3106716/1‑150 (MQ=255)
                                                                                                gTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACt                                              <  1:463921/150‑1 (MQ=255)
                                                                                                 ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACGATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTa                                             <  2:4157170/150‑1 (MQ=255)
                                                                                                  tCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTag                                            <  1:748033/150‑2 (MQ=255)
                                                                                                   cTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                           >  1:1855793/1‑148 (MQ=255)
                                                                                                    tGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTagag                                          >  1:4019798/1‑147 (MQ=255)
                                                                                                       aagaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgagaag                                       >  2:719345/1‑150 (MQ=255)
                                                                                                        agaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGt                                      >  1:2582689/1‑150 (MQ=255)
                                                                                                            gCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTa                                  >  1:2276471/1‑150 (MQ=255)
                                                                                                                gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                              <  1:335512/150‑1 (MQ=255)
                                                                                                                gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                              >  1:3440036/1‑150 (MQ=255)
                                                                                                                gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                              >  2:3287881/1‑150 (MQ=255)
                                                                                                                 aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAt                             <  1:3117313/150‑1 (MQ=255)
                                                                                                                 aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAt                             >  1:3761097/1‑150 (MQ=255)
                                                                                                                  gCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAtt                            >  1:2176223/1‑150 (MQ=255)
                                                                                                                    agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                          >  1:3267976/1‑150 (MQ=255)
                                                                                                                    agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                          >  2:638332/1‑150 (MQ=255)
                                                                                                                      aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                        <  1:3901671/150‑1 (MQ=255)
                                                                                                                      aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                        <  1:912929/150‑1 (MQ=255)
                                                                                                                      aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                        <  1:1327637/150‑1 (MQ=255)
                                                                                                                       agaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCa                       <  2:3998206/150‑1 (MQ=255)
                                                                                                                             tttCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGtt                 <  1:4260693/150‑1 (MQ=255)
                                                                                                                               tCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTAt               <  1:618357/150‑1 (MQ=255)
                                                                                                                                ccAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATc                    >  1:4123342/1‑144 (MQ=255)
                                                                                                                                  aaGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTa            <  2:1547025/150‑1 (MQ=255)
                                                                                                                                  aaGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTa            >  2:1086084/1‑150 (MQ=255)
                                                                                                                                  aaGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTa            <  1:2331500/150‑1 (MQ=255)
                                                                                                                                     aaaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa         <  2:3641522/150‑1 (MQ=255)
                                                                                                                                     aaaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa         >  2:787435/1‑150 (MQ=255)
                                                                                                                                      aaagtttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATag        <  2:2820326/146‑1 (MQ=255)
                                                                                                                                       aagtttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATaga       <  2:2599491/147‑1 (MQ=255)
                                                                                                                                           ttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGaa   <  2:2040112/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GGCTCGTAACTTAGGGTTATTAATAGAGGTCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAT  >  CP043523/1867489‑1867778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑