Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,867,671 C→T L49F (CTC→TTT)  FTN78_chr1914 → Site‑specific recombinase, DNA invertase Pin related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,867,6710CT99.7% 1195.0 / NA 359L49F (CTC→TTT) FTN78_chr1914Site‑specific recombinase, DNA invertase Pin related protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (185/173);  total (186/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

AAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTTATATATTAAATATTGGACTTA  >  CP043523/1867528‑1867807
                                                                                                                                               |                                                                                                                                        
aaaaTTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAg                                                                                                                                    >  2:241059/1‑150 (MQ=255)
 ctgttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCATATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTAGTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGt                                                                                                                                   >  2:1829457/4‑150 (MQ=255)
    ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGCAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATTTAGTATTTAAAAAGTTaa                                                                                                                                <  2:2809675/150‑1 (MQ=255)
    ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaa                                                                                                                                <  1:4116172/150‑1 (MQ=255)
    ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTCTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaa                                                                                                                                <  2:4033878/150‑1 (MQ=255)
     tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                                               >  1:1547025/1‑149 (MQ=255)
     tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                                               <  1:304665/150‑2 (MQ=255)
     tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                                               <  1:95738/150‑2 (MQ=255)
      gggTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaag                                                                                                                              <  2:348165/150‑3 (MQ=255)
      gggTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaag                                                                                                                              >  1:1878045/1‑148 (MQ=255)
          aTGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggatg                                                                                                                          >  2:1096112/1‑144 (MQ=255)
                        aCTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAAtt                                                                                                            >  1:4308158/1‑150 (MQ=255)
                          ttttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAGGGATGGTGACACTTTAATtgt                                                                                                          <  1:2911110/150‑1 (MQ=255)
                          ttttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgt                                                                                                          >  1:3979612/1‑150 (MQ=255)
                            ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGa                                                                                                        >  2:2197033/1‑150 (MQ=255)
                            ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGa                                                                                                        >  2:3491824/1‑150 (MQ=255)
                            ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGa                                                                                                        >  2:1615355/1‑150 (MQ=255)
                              gAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTCAAAAAGTTAAAGGATGGTGACACTCTAACTGTGACa                                                                                                      >  2:1340637/1‑150 (MQ=255)
                                aCAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaaa                                                                                                    >  1:4593346/1‑150 (MQ=255)
                                 caaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAg                                                                                                   <  2:1306822/147‑1 (MQ=255)
                                 caaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAg                                                                                                   >  2:2163536/4‑150 (MQ=255)
                                  aaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGt                                                                                                  >  2:529257/3‑150 (MQ=255)
                                   aaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGtt                                                                                                 >  2:786730/2‑150 (MQ=255)
                                     aaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAg                                                                                               <  2:1878218/150‑1 (MQ=255)
                                     aaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAg                                                                                               <  1:2006139/150‑1 (MQ=255)
                                      aTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGa                                                                                              >  2:2439307/1‑150 (MQ=255)
                                        tAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGacc                                                                                            <  1:4412446/150‑3 (MQ=255)
                                         aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGaccg                                                                                           >  1:225221/1‑147 (MQ=255)
                                         aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCg                                                                                           <  1:2153985/150‑1 (MQ=255)
                                           aaTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAt                                                                                         >  1:3115261/1‑150 (MQ=255)
                                            aTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAtt                                                                                        <  1:2366786/150‑1 (MQ=255)
                                            aTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAtt                                                                                        >  1:1975276/1‑150 (MQ=255)
                                             tctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTa                                                                                       <  1:2989103/150‑1 (MQ=255)
                                              ctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAg                                                                                      >  1:3390027/1‑150 (MQ=255)
                                              ctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAg                                                                                      >  1:1839710/1‑150 (MQ=255)
                                                  aaTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCGAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAg                                                                                  >  1:3995288/1‑150 (MQ=255)
                                                   nttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAgg                                                                                 >  2:947998/2‑150 (MQ=255)
                                                   aTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAgg                                                                                 >  2:638848/1‑150 (MQ=255)
                                                    ttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGa                                                                                <  2:3019804/150‑1 (MQ=255)
                                                    ttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGa                                                                                <  1:3743522/150‑1 (MQ=255)
                                                     tGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGaa                                                                               >  2:3117332/1‑150 (MQ=255)
                                                     tGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGaa                                                                               >  1:1583104/1‑150 (MQ=255)
                                                      gAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGTACAGTTTTCTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATCTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAATTTAGACCGATTAGCCAGGAac                                                                              <  2:4450628/150‑1 (MQ=255)
                                                       aaGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAaca                                                                             <  2:1957415/150‑1 (MQ=255)
                                                         gTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACt                                                                           <  2:1892079/150‑1 (MQ=255)
                                                           tCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTag                                                                         <  1:748033/150‑2 (MQ=255)
                                                            cTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                                                        >  2:1706947/1‑148 (MQ=255)
                                                            cTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTAGTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                                                        <  1:2096898/150‑3 (MQ=255)
                                                             tGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTagag                                                                       >  1:656401/1‑147 (MQ=255)
                                                             tGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTagag                                                                       <  1:125481/150‑1 (MQ=255)
                                                              gAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgaga                                                                      >  1:2563411/1‑150 (MQ=255)
                                                                   aagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGttt                                                                 <  2:2747617/150‑1 (MQ=255)
                                                                     gCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTa                                                               <  1:577042/150‑1 (MQ=255)
                                                                     gCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTa                                                               >  1:2276471/1‑150 (MQ=255)
                                                                      cAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAg                                                              >  2:673405/1‑150 (MQ=255)
                                                                       aGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGa                                                             <  1:170910/150‑1 (MQ=255)
                                                                       aGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGa                                                             >  1:1059678/1‑150 (MQ=255)
                                                                       aGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGa                                                             >  1:4156189/1‑150 (MQ=255)
                                                                         gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                                                           >  2:3287881/1‑150 (MQ=255)
                                                                         gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                                                           >  2:809159/1‑150 (MQ=255)
                                                                          aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAt                                                          <  1:2189582/150‑1 (MQ=255)
                                                                          aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAt                                                          >  1:3761097/1‑150 (MQ=255)
                                                                            cAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTg                                                        >  2:2303986/1‑150 (MQ=255)
                                                                             agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                                                       >  1:3256928/1‑150 (MQ=255)
                                                                              gaagaaGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATGTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGtt                                                      >  2:2823382/1‑150 (MQ=255)
                                                                              gaagaaGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGAGACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGtn                                                      <  2:4156189/150‑2 (MQ=255)
                                                                              gaagaaGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGtt                                                      <  1:4517411/150‑1 (MQ=255)
                                                                               aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                                                     <  1:1327637/150‑1 (MQ=255)
                                                                               aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                                                     <  2:2607028/150‑1 (MQ=255)
                                                                                   agTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATc                                                 <  2:4566599/150‑1 (MQ=255)
                                                                                     ttttCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGt                                               <  1:346444/150‑1 (MQ=255)
                                                                                      tttCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGtt                                              <  2:1018003/150‑1 (MQ=255)
                                                                                       ttCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTa                                             <  2:2116338/150‑1 (MQ=255)
                                                                                           aaGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTa                                         <  1:2331500/150‑1 (MQ=255)
                                                                                            aGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTaa                                        <  2:121967/150‑1 (MQ=255)
                                                                                              aaaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa                                      <  2:3641522/150‑1 (MQ=255)
                                                                                                     tactggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAt                               >  1:1923610/8‑150 (MQ=255)
                                                                                                     tACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAt                               <  2:4119408/150‑1 (MQ=255)
                                                                                                      actggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAtt                              >  1:4062326/7‑150 (MQ=255)
                                                                                                      actggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAtt                              <  2:4308158/144‑1 (MQ=255)
                                                                                                      actggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAtt                              <  2:4482593/144‑1 (MQ=255)
                                                                                                       ctggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTa                             <  2:3908393/145‑1 (MQ=255)
                                                                                                        tggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTaa                            >  1:2896298/5‑150 (MQ=255)
                                                                                                         ggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAg                           >  1:876843/4‑150 (MQ=255)
                                                                                                         ggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAg                           >  1:873743/4‑150 (MQ=255)
                                                                                                          gaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAgg                          <  2:3699760/148‑1 (MQ=255)
                                                                                                           aaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTCAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTCTTCAATCGTTATTTAATAGAGGAATTAAGGt                         <  2:3984456/149‑1 (MQ=255)
                                                                                                           aaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGt                         >  2:4070305/2‑150 (MQ=255)
                                                                                                              tACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGttca                      >  2:2595162/1‑148 (MQ=255)
                                                                                                               aCAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGttcat                     >  1:3897691/1‑147 (MQ=255)
                                                                                                               aCAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcat                     <  2:589703/150‑1 (MQ=255)
                                                                                                                  gtcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcatata                  <  1:1147430/147‑1 (MQ=255)
                                                                                                                  gtcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcatata                  <  2:237289/147‑1 (MQ=255)
                                                                                                                   tcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCatatat                 <  2:2940872/148‑1 (MQ=255)
                                                                                                                     gAACGACCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTATAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTa               <  1:3346449/150‑1 (MQ=255)
                                                                                                                       aCGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTaaa             <  2:1460732/150‑1 (MQ=255)
                                                                                                                        cGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAat            <  1:2760024/150‑1 (MQ=255)
                                                                                                                           ccACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATAtt         <  2:1548952/150‑1 (MQ=255)
                                                                                                                             acaATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTgg       >  2:2074386/1‑150 (MQ=255)
                                                                                                                               aatttaatctAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAGTCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTggtc     >  1:3595806/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                        
AAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTTATATATTAAATATTGGACTTA  >  CP043523/1867528‑1867807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑