Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,883,262 3 bp→TTA intergenic (+143/‑16) FTN78_chr1927 → / → FTN78_chr1928 3‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,883,2620CT99.8% 1456.9 / ‑4.5 435intergenic (+143/‑18)FTN78_chr1927/FTN78_chr19283‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (199/235);  minor base G (0/1);  total (199/236)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01
*CP0435231,883,2630AT99.8% 1465.1 / NA 435intergenic (+144/‑17)FTN78_chr1927/FTN78_chr19283‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (198/236);  minor base G (1/0);  total (199/236)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0435231,883,2640CA100.0% 1454.4 / NA 435intergenic (+145/‑16)FTN78_chr1927/FTN78_chr19283‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (199/236);  total (199/236)

Only 100 of 435 total aligned reads displayed.

GAAAGCCATCT‑TAGACTCCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAA  >  CP043523/1883124‑1883400
                                                                                                                                           |||                                                                                                                                        
 aaaGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTgag                                                                                                                                 <  2:281831/150‑1 (MQ=255)
    gCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGAtt                                                                                                                              <  2:2589041/150‑1 (MQ=255)
    gCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGAtt                                                                                                                              <  2:2132443/150‑1 (MQ=255)
    gCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGAtt                                                                                                                              <  2:1112641/150‑1 (MQ=255)
    gCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGAtt                                                                                                                              <  1:4248459/150‑1 (MQ=255)
      catctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTta                                                                                                                            >  2:428589/7‑150 (MQ=255)
      catctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTta                                                                                                                            >  2:2001711/7‑150 (MQ=255)
      catctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTta                                                                                                                            <  1:2014811/144‑1 (MQ=255)
       atctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTAGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTtat                                                                                                                           >  1:3458754/6‑150 (MQ=255)
        tctatAGACTCCGATTTGAACACACTACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTtata                                                                                                                          <  1:675378/146‑1 (MQ=255)
            tAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGATAAAATTACTCCTTGAGATTTATATGaa                                                                                                                      >  1:4073372/1‑150 (MQ=255)
            tAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaa                                                                                                                      <  1:351661/150‑1 (MQ=255)
             aGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                     <  1:1712374/150‑1 (MQ=255)
             aGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                     >  1:229186/1‑150 (MQ=255)
             aGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                     <  2:106478/150‑1 (MQ=255)
             aGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                     <  2:3211948/150‑1 (MQ=255)
             aGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                     >  2:4582807/1‑150 (MQ=255)
              gACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAg                                                                                                                    >  2:950621/1‑150 (MQ=255)
                cTCCGATTTGAACACACGTCAGCTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGTGGCCTTCGAAGCGTGAAGTAGAAAATCATCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGct                                                                                                                  <  2:4230030/150‑2 (MQ=255)
                  ccGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaa                                                                                                                >  2:4145660/1‑150 (MQ=255)
                   cGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaag                                                                                                               <  1:307722/150‑1 (MQ=255)
                     aTTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaa                                                                                                             <  2:3326335/150‑1 (MQ=255)
                       ttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaa                                                                                                           <  2:236050/150‑1 (MQ=255)
                        tGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaag                                                                                                          >  1:1147124/1‑150 (MQ=255)
                           acacacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                       <  1:259758/150‑1 (MQ=255)
                            cacacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAtt                                                                                                      >  2:2240268/1‑150 (MQ=255)
                             acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTg                                                                                                     <  2:382385/150‑1 (MQ=255)
                              cacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTgg                                                                                                    >  1:1550533/1‑150 (MQ=255)
                                cGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGaa                                                                                                  <  1:1481900/150‑1 (MQ=255)
                                cGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGaa                                                                                                  <  2:1314842/150‑1 (MQ=255)
                                 gACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAAc                                                                                                 <  2:3577033/150‑1 (MQ=255)
                                    ngttaTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACgag                                                                                              >  2:497499/5‑150 (MQ=255)
                                      ttaTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGagag                                                                                            >  2:43204/3‑150 (MQ=255)
                                      ttaTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGagag                                                                                            <  2:3018185/148‑1 (MQ=255)
                                       taTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGc                                                                                           <  1:557600/149‑1 (MQ=255)
                                         tGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtt                                                                                         <  2:2793692/150‑1 (MQ=255)
                                         tGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtt                                                                                         <  2:72058/150‑1 (MQ=255)
                                           cgctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGTGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtttt                                                                                       <  2:100567/148‑1 (MQ=255)
                                            tctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTa                                                                                      <  2:2526386/150‑1 (MQ=255)
                                             ctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaa                                                                                     >  2:3541509/1‑150 (MQ=255)
                                             ctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaa                                                                                     >  1:3604615/1‑150 (MQ=255)
                                              tGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaa                                                                                    <  2:2182528/150‑1 (MQ=255)
                                               gTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaaa                                                                                   <  1:1891063/150‑1 (MQ=255)
                                                 cGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATc                                                                                 <  1:1746202/150‑1 (MQ=255)
                                                 cGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATc                                                                                 >  1:2368123/1‑150 (MQ=255)
                                                  gtgtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCa                                                                                >  1:4494457/1‑150 (MQ=255)
                                                    gtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAtt                                                                              <  1:2887433/150‑1 (MQ=255)
                                                     ttttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttc                                                                             <  1:1437984/150‑1 (MQ=255)
                                                      tttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttct                                                                            <  1:3322583/150‑1 (MQ=255)
                                                       ttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttctt                                                                           >  2:168648/1‑150 (MQ=255)
                                                        tACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttcttc                                                                          <  2:622360/150‑1 (MQ=255)
                                                         aCATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATtcttct                                                                         >  2:866717/1‑150 (MQ=255)
                                                         aCATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATtcttct                                                                         <  2:3915430/150‑1 (MQ=255)
                                                          cATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTg                                                                        >  1:1046528/1‑150 (MQ=255)
                                                            tGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAGAATCAACGATTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                      >  2:4485293/1‑150 (MQ=255)
                                                            tGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                      <  1:1650016/150‑1 (MQ=255)
                                                            tGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                      <  1:376010/150‑1 (MQ=255)
                                                             gTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGt                                                                     <  2:1664198/150‑1 (MQ=255)
                                                              tCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTg                                                                    <  2:2049154/150‑1 (MQ=255)
                                                              tCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGCGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTg                                                                    >  2:1885013/1‑150 (MQ=255)
                                                                 aTCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGAtt                                                                 >  1:1782808/1‑150 (MQ=255)
                                                                   cGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTAt                                                               >  1:100141/1‑150 (MQ=255)
                                                                          cctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTa                                                        >  1:4140862/8‑150 (MQ=255)
                                                                           ctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTaa                                                       >  1:2230313/7‑150 (MQ=255)
                                                                            taCTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAat                                                      >  2:1708968/2‑150 (MQ=255)
                                                                             actgtaACTTCATGAACAAGAGGCCTTCGAAGCGTGAAGTTGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAata                                                     <  1:4362895/146‑1 (MQ=255)
                                                                             actgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAata                                                     <  1:2718951/146‑1 (MQ=255)
                                                                             aCTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAata                                                     >  2:3268810/1‑150 (MQ=255)
                                                                              ctgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAatat                                                    <  1:3875190/147‑1 (MQ=255)
                                                                                  aaCTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCt                                                <  1:3830902/150‑1 (MQ=255)
                                                                                  aaCTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCt                                                <  2:3525486/150‑1 (MQ=255)
                                                                                   ncTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTg                                               >  1:91587/2‑150 (MQ=255)
                                                                                   aCTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTg                                               <  2:115272/150‑1 (MQ=255)
                                                                                     ttcataAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGaa                                             <  2:3049501/147‑1 (MQ=255)
                                                                                       cataAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAgg                                           >  2:3469553/2‑150 (MQ=255)
                                                                                         taAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGcc                                         <  2:1456487/150‑2 (MQ=255)
                                                                                          aaaCAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGccg                                        <  2:759128/150‑3 (MQ=255)
                                                                                             cAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTAt                                     >  1:4012555/1‑150 (MQ=255)
                                                                                              aaGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATc                                    <  1:591484/150‑1 (MQ=255)
                                                                                               agagGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCt                                   <  1:3216028/150‑1 (MQ=255)
                                                                                                   gCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAg                               >  2:4002491/1‑150 (MQ=255)
                                                                                                   gCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAg                               <  2:1778556/150‑1 (MQ=255)
                                                                                                   gCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAg                               <  1:489123/150‑1 (MQ=255)
                                                                                                    ccTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGa                              <  2:2429075/150‑1 (MQ=255)
                                                                                                    ccTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGa                              <  1:2814957/150‑1 (MQ=255)
                                                                                                          aaGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGGTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtctc                        >  1:1579177/1‑150 (MQ=255)
                                                                                                          aaGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtctc                        >  2:3379375/1‑150 (MQ=255)
                                                                                                           aGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCa                       >  2:525242/1‑150 (MQ=255)
                                                                                                              gTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGt                    >  2:3627783/1‑150 (MQ=255)
                                                                                                              gTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGt                    >  1:2140455/1‑150 (MQ=255)
                                                                                                                gAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCt                  <  1:3020942/150‑1 (MQ=255)
                                                                                                                gAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCAAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAGTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCt                  >  2:4075749/1‑150 (MQ=255)
                                                                                                                   gTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATg               >  1:2456703/1‑150 (MQ=255)
                                                                                                                   gTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATg               >  2:2744487/1‑150 (MQ=255)
                                                                                                                      gaaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGaca            <  1:950621/149‑1 (MQ=255)
                                                                                                                        aaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACt          <  1:2526753/150‑1 (MQ=255)
                                                                                                                           tCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTcgc       >  1:4303541/1‑148 (MQ=255)
                                                                                                                            cAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCt      <  1:1300299/150‑1 (MQ=255)
                                                                                                                            cAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCt      <  1:2439325/150‑1 (MQ=255)
                                                                                                                                gCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTaaaa  <  1:85380/150‑1 (MQ=255)
                                                                                                                                           |||                                                                                                                                        
GAAAGCCATCT‑TAGACTCCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAA  >  CP043523/1883124‑1883400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑