BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,848,324	3 bp→TAC	coding (33‑35/201 nt)	FTN78_chr1898 →	Cold shock protein of CSP family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,848,324	0	A	T	98.5%	222.6 / ‑4.8	136	A11A (GCA→GCT)	FTN78_chr1898	Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (88/46); total (88/48)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP043523	1,848,325	0	G	A	98.5%	224.8 / ‑5.3	136	D12T (GAT→ACT)	FTN78_chr1898	Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (88/46); total (88/48)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP043523	1,848,326	0	A	C	97.8%	222.2 / ‑4.8	136	D12T (GAT→ACT)	FTN78_chr1898	Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base A (1/2); new base C (87/46); total (88/48)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATAA‑‑ATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAA  >  CP043523/1848185‑1848447

|||

taATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAgg                                                                                                                     >  2:2336980/1‑150 (MQ=14)

   ttAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                                                                                  <  2:1475175/150‑1 (MQ=11)

   ttAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                                                                                  <  2:1475039/150‑1 (MQ=14)

   ttAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                                                                                  >  2:1074656/1‑150 (MQ=14)

    tAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAttt                                                                                                                 >  2:2349945/1‑150 (MQ=14)

    tAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAttt                                                                                                                 >  2:2349879/1‑150 (MQ=11)

       tgtTATTATAAAGAAGTAGATTTTGTCATTAGTATAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATTGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                                                                                              <  2:4505706/150‑2 (MQ=11)

       tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAACTGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                                                                                              >  1:1030530/1‑149 (MQ=11)

       tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                                                                                              <  2:4237839/150‑2 (MQ=11)

       tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                                                                                              >  2:1346135/1‑149 (MQ=11)

        gtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggtt                                                                                                             >  2:2051800/1‑148 (MQ=11)

        gtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggtt                                                                                                             >  2:716033/1‑148 (MQ=11)

         ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                                                                                            <  2:260169/150‑1 (MQ=11)

         ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                                                                                            >  2:1004747/1‑150 (MQ=11)

         ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                                                                                            >  1:3168498/1‑150 (MQ=11)

         ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                                                                                            >  1:1741541/1‑150 (MQ=11)

         ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCAAATTTCGAAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                                                                                            <  2:3416525/150‑1 (MQ=11)

          tattatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTgttttt                                                                                                           <  2:2242952/150‑6 (MQ=11)

          tattatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                                                                                           <  2:3663241/150‑1 (MQ=11)

          tattatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                                                                                           >  2:2446328/1‑150 (MQ=11)

            ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAt                                                                                                         <  1:4503986/150‑1 (MQ=11)

             tataAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAtt                                                                                                        >  1:3648906/1‑149 (MQ=11)

             tataAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAtt                                                                                                        >  2:2451788/1‑149 (MQ=11)

              ataaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAtta                                                                                                       <  2:1971755/147‑3 (MQ=11)

              ataaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAtta                                                                                                       >  1:3809112/4‑148 (MQ=11)

               taaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttac                                                                                                      >  2:191472/3‑147 (MQ=11)

               taaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttac                                                                                                      >  2:1163905/3‑147 (MQ=11)

               taaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttac                                                                                                      >  2:3714670/3‑147 (MQ=11)

                aaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacc                                                                                                     >  2:1704977/2‑146 (MQ=11)

                aaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacc                                                                                                     >  2:1116383/2‑146 (MQ=11)

                aaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑ATATAGT‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACt                                                                                                     >  2:1112688/2‑150 (MQ=11)

                 aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                                                                                    >  2:1046277/1‑145 (MQ=11)

                 aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAcct                                                                                                    <  2:735366/150‑3 (MQ=11)

                 aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAcct                                                                                                    <  2:735702/150‑3 (MQ=11)

                  agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacctc                                                                                                   >  2:2624418/1‑144 (MQ=11)

                  agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacctc                                                                                                   >  2:2864756/1‑144 (MQ=11)

                  agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacctc                                                                                                   >  2:3313877/1‑144 (MQ=11)

                  agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacctc                                                                                                   >  2:2864875/1‑144 (MQ=11)

                        agaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                                                                                             >  1:1448165/3‑150 (MQ=11)

                        agaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                                                                                             >  1:4211915/3‑150 (MQ=11)

                         gaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagat                                                                                            >  1:1224109/2‑149 (MQ=11)

                         gaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagat                                                                                            <  2:3086872/149‑2 (MQ=11)

                         gaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagat                                                                                            <  2:3502652/149‑2 (MQ=11)

                         gaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagat                                                                                            <  2:898395/149‑2 (MQ=11)

                          aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                                                                                           <  1:4198594/150‑3 (MQ=11)

                          aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                                                                                           <  1:3364956/150‑3 (MQ=11)

                          aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                                                                                           <  1:3362684/150‑3 (MQ=11)

                           ttttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATGTTGTCGAAAGCAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAAAGGTTTAACGCTAATAAAGGATTTGGTCTTATAACCTCTGAAGAggg                                                                                          >  2:1633014/1‑150 (MQ=11)

                             ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtc                                                                                        >  1:4323904/1‑145 (MQ=11)

                             ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtc                                                                                        <  2:2694156/150‑3 (MQ=11)

                             ttGTCATGAGTAAAATGTTTTATACATTCACTTTTCGAAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtc                                                                                        <  2:4543498/150‑3 (MQ=11)

                              tGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATACGATTTACAATTTTATCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtca                                                                                       >  2:3881048/1‑147 (MQ=11)

                              tGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtca                                                                                       >  1:2494871/1‑144 (MQ=11)

                              tGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtca                                                                                       <  2:476938/150‑7 (MQ=11)

                               gTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                                                                                      <  2:2239668/150‑8 (MQ=11)

                               gTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                                                                                      <  2:1750945/150‑8 (MQ=11)

                               gTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                                                                                      <  2:1746860/150‑8 (MQ=11)

                               gTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                                                                                      <  2:3046944/150‑8 (MQ=11)

                                  aTGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCAGAAGAAGGCAAAGAt                                                                                   >  2:3733735/1‑150 (MQ=11)

                                    gAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGAttt                                                                                 >  2:4482696/1‑150 (MQ=11)

                                    gAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGAttt                                                                                 >  1:2150218/1‑150 (MQ=11)

                                    gAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGAttt                                                                                 >  1:2264538/1‑150 (MQ=11)

                                     aGTAACATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACATTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTg                                                                                >  2:4280462/1‑150 (MQ=2)

                                      gTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGt                                                                               >  1:1574065/1‑150 (MQ=11)

                                       tAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTTAAGATGGTCAAGATTTGtt                                                                              <  2:4136400/150‑1 (MQ=11)

                                       tAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑ATATA‑TCATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGtt                                                                              <  2:4137598/150‑1 (MQ=11)

                                        aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc                                                                             >  1:12133/1‑150 (MQ=11)

                                        aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc                                                                             >  2:137802/1‑150 (MQ=11)

                                        aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc                                                                             >  2:3103549/1‑150 (MQ=11)

                                        aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc                                                                             >  2:3245014/1‑150 (MQ=11)

                                         aaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCg                                                                            >  2:2794809/1‑150 (MQ=11)

                                         aaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCg                                                                            >  1:2666421/1‑150 (MQ=11)

                                          aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGc                                                                           <  2:251987/150‑1 (MQ=11)

                                          aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGc                                                                           >  2:1551902/1‑150 (MQ=11)

                                            tGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACTTCTGAAGATGGTCAAGATTTGTTCGCTc                                                                         <  2:3849627/150‑1 (MQ=11)

                                             gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATTGTCAAGATTTGTTCGCTCa                                                                        <  2:4108100/150‑1 (MQ=11)

                                             gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCa                                                                        >  2:3143518/1‑150 (MQ=11)

                                             gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCa                                                                        >  2:257850/1‑150 (MQ=11)

                                             gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCa                                                                        >  2:646039/1‑150 (MQ=11)

                                             gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCa                                                                        >  2:644541/1‑150 (MQ=11)

                                              ttttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc                                                                       >  1:540250/1‑150 (MQ=11)

                                               tttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACt                                                                      <  1:1287900/150‑1 (MQ=11)

                                                ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACtt                                                                     >  1:3608272/1‑150 (MQ=11)

                                                ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACtt                                                                     >  2:3842945/1‑150 (MQ=11)

                                                ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGACTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACtt                                                                     >  1:3596243/1‑150 (MQ=11)

                                                 tataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtc                                                                    >  1:1795941/1‑150 (MQ=11)

                                                 tataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtc                                                                    >  1:3699607/1‑150 (MQ=11)

                                                 tataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtc                                                                    <  2:1782186/150‑1 (MQ=11)

                                                  ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct                                                                   >  2:935224/1‑150 (MQ=11)

                                                  ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct                                                                   <  2:3008814/150‑1 (MQ=11)

                                                  ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct                                                                   >  2:2568421/1‑150 (MQ=11)

                                                  ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct                                                                   >  2:2567785/1‑150 (MQ=11)

                                                  ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct                                                                   >  1:1630965/1‑150 (MQ=11)

                                                   taGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtctc                                                                  >  1:3913750/1‑150 (MQ=11)

                                                   taGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtctc                                                                  >  1:1795768/1‑150 (MQ=11)

                                                   taGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtctc                                                                  >  1:1582742/1‑150 (MQ=11)

                                                   taGACTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtctc                                                                  <  1:2986538/150‑1 (MQ=2)

                                                    aGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCa                                                                 >  1:15913/1‑150 (MQ=11)

                                                                                              tgAACTAA‑‑ATAT‑‑T‑ATAGAAAATTAAACATTAATATGGTTTAACGCAGATAAAAGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTATTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCaaaa                     <  2:2219729/149‑1 (MQ=11)

                                                                                                                      aaTGGAACAGTAAAATGGTTTAAAGCAGATAAAGGATTTGGCTTTATAACTTCAGAAGAAGGAAAAGATTTGTTCGCTCACTTCTAAGAAATCAAATCTGATGGATTCAAAAAAAATAATGAAAATAAAAAAGTTGAATTTGATGTTgaa  <  2:2630883/150‑1 (MQ=11)

|||

TAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATAA‑‑ATAT‑‑T‑ATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAA  >  CP043523/1848185‑1848447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`