Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,867,931 2 bp→GT coding (407‑408/585 nt) FTN78_chr1914 → Site‑specific recombinase, DNA invertase Pin related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,867,9310AG99.8% 1570.4 / NA 441N136S (AAC→AGT) FTN78_chr1914Site‑specific recombinase, DNA invertase Pin related protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (237/202);  total (238/203)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0435231,867,9320CT99.8% 1434.4 / NA 441N136S (AAC→AGT) FTN78_chr1914Site‑specific recombinase, DNA invertase Pin related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (236/203);  minor base G (1/0);  total (238/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 441 total aligned reads displayed.

TATATTAAATATTGGACTTATCGATAATACACCGACAGGTCAACTTATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAACTTTGCTAAAATCCATGATCCGAACTTTAAAGAAGGACGTCCTCAGAAATTTACTGAAGAACAAATTCAATTTGCTTATGAATTAAAGCAGCAAGGTATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGACTCAAAAAAGAAGATTTAT  >  CP043523/1867788‑1868069
                                                                                                                                               ||                                                                                                                                         
     tAAATATTGGTCTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAaggaag                                                                                                                                 <  1:690118/150‑1 (MQ=255)
         tatTGGTCTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTaaggaagggcg                                                                                                                             >  2:1228885/1‑147 (MQ=255)
            tggtcTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTcc                                                                                                                          <  1:2226607/146‑1 (MQ=255)
            tGGTCTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTcc                                                                                                                          >  2:2916168/1‑150 (MQ=255)
             ggtcTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCGAAGTTTTAAGGAAGGGCGTCCt                                                                                                                         >  1:3418562/4‑150 (MQ=255)
             ggtcTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCt                                                                                                                         <  1:1135215/147‑1 (MQ=255)
             ggtcTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCt                                                                                                                         >  1:639493/4‑150 (MQ=255)
              gtcTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTc                                                                                                                        >  1:1760271/3‑150 (MQ=255)
              gtcTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTc                                                                                                                        <  2:339898/148‑1 (MQ=255)
                cTTATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCaa                                                                                                                      >  2:1549814/1‑149 (MQ=255)
                  tATTGATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCaaaa                                                                                                                    >  2:1648591/1‑147 (MQ=255)
                   attgATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAaaaa                                                                                                                   <  1:2199225/147‑1 (MQ=255)
                     tgATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAAtt                                                                                                                 <  2:1460812/149‑1 (MQ=255)
                     tgATAATACACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAAtt                                                                                                                 <  1:2074386/149‑1 (MQ=255)
                        taataCACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTAc                                                                                                              <  2:2345789/150‑1 (MQ=255)
                         aataCACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTAcc                                                                                                             >  1:569432/1‑149 (MQ=255)
                         aataCACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTAcc                                                                                                             <  1:2456464/150‑2 (MQ=255)
                           taCACCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTAccga                                                                                                           >  1:3828289/1‑147 (MQ=255)
                              acctactggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCgaaga                                                                                                        >  2:2427633/8‑150 (MQ=255)
                              acctactggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCgaaga                                                                                                        >  2:2641205/8‑150 (MQ=255)
                              acCTACTGGTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCgaaga                                                                                                        >  2:3968286/1‑150 (MQ=255)
                               cctactggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCgaagaa                                                                                                       >  2:2387783/7‑150 (MQ=255)
                               cctactggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCgaagaa                                                                                                       >  1:1187211/7‑150 (MQ=255)
                                ctactggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAAc                                                                                                      >  1:2343253/6‑150 (MQ=255)
                                  actggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACaa                                                                                                    >  2:78628/4‑150 (MQ=255)
                                   ctggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACaaa                                                                                                   >  1:4531680/3‑150 (MQ=255)
                                     ggTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAAtt                                                                                                 <  2:4336209/150‑1 (MQ=255)
                                      gTCAGCTGATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTc                                                                                                >  2:1154989/1‑150 (MQ=255)
                                        cagctgaTTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCaa                                                                                              <  2:489026/144‑1 (MQ=255)
                                         agctgaTTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAAt                                                                                             >  2:109958/6‑150 (MQ=255)
                                            tgaTTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTg                                                                                          >  1:1997741/3‑150 (MQ=255)
                                              aTTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCt                                                                                        >  1:1744548/1‑150 (MQ=255)
                                                 tttACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTAt                                                                                     <  1:2372743/150‑1 (MQ=255)
                                                   tACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGa                                                                                   <  1:825113/150‑1 (MQ=255)
                                                    aCCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGag                                                                                  >  2:4127244/1‑149 (MQ=255)
                                                    aCCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGag                                                                                  <  1:1658004/150‑2 (MQ=255)
                                                     ccATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGagt                                                                                 <  2:1132781/150‑3 (MQ=255)
                                                     ccATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGagt                                                                                 >  1:4510897/1‑148 (MQ=255)
                                                        tCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTaa                                                                              <  1:468517/150‑1 (MQ=255)
                                                         cTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTaaa                                                                             >  1:2720440/1‑150 (MQ=255)
                                                         cTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTaaa                                                                             >  1:1971189/1‑150 (MQ=255)
                                                             aGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAANCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAaacag                                                                         >  2:2764027/1‑150 (MQ=255)
                                                              gTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAaacagc                                                                        <  1:926671/150‑1 (MQ=255)
                                                                  ttttGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACATATTCAATTTGCTCATGAGTTAAAACAGCAAgg                                                                    >  1:2054548/1‑150 (MQ=255)
                                                                  ttttGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAgg                                                                    >  1:3860859/1‑150 (MQ=255)
                                                                  ttttGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAgg                                                                    <  2:3794887/150‑1 (MQ=255)
                                                                  ttttGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAgg                                                                    >  1:2111132/1‑150 (MQ=255)
                                                                  ttttGCGCAGTGTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAgg                                                                    >  2:4057481/1‑150 (MQ=255)
                                                                   tttGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAgga                                                                   <  1:3515642/150‑2 (MQ=255)
                                                                   tttGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAgga                                                                   <  1:3522615/150‑2 (MQ=255)
                                                                    ttGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAggaa                                                                  >  1:3144764/1‑148 (MQ=255)
                                                                          aGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACtt                                                            >  2:2527076/1‑150 (MQ=255)
                                                                          aGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACtt                                                            <  1:1095616/150‑1 (MQ=255)
                                                                            tttGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTAc                                                          >  1:737908/1‑150 (MQ=255)
                                                                               gAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAg                                                       >  1:2763168/1‑150 (MQ=255)
                                                                                aaCGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAga                                                      >  1:1311986/1‑150 (MQ=255)
                                                                                  cGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAgatg                                                    <  1:3639276/150‑1 (MQ=255)
                                                                                  cGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAgatg                                                    >  2:1757652/1‑150 (MQ=255)
                                                                                    agaCCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAgatgat                                                  <  1:4331185/150‑1 (MQ=255)
                                                                                    agaCCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACACCAAGGAATGACTTACAAgatgat                                                  >  2:3731515/1‑150 (MQ=255)
                                                                                     gaCCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGAtt                                                 >  2:360577/1‑150 (MQ=255)
                                                                                     gaCCTTATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGAtt                                                 <  2:2201949/150‑1 (MQ=255)
                                                                                       ccTTATTGTACCCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGa                                               <  1:4397445/150‑1 (MQ=255)
                                                                                          tATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCg                                            <  1:3251044/150‑1 (MQ=255)
                                                                                          tATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCg                                            <  1:3003279/150‑1 (MQ=255)
                                                                                          tATTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCg                                            >  2:1293231/1‑150 (MQ=255)
                                                                                           aTTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGa                                           <  2:2705473/150‑1 (MQ=255)
                                                                                           aTTGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGa                                           >  1:3098520/1‑150 (MQ=255)
                                                                                            ttGTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGaa                                          <  2:1848064/150‑1 (MQ=255)
                                                                                              gTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGaaaa                                        >  2:188629/1‑150 (MQ=255)
                                                                                              gTAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGaaaa                                        <  1:4221995/150‑1 (MQ=255)
                                                                                               tAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGaaaaa                                       >  1:3668199/1‑150 (MQ=255)
                                                                                               tAACCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGaaaaa                                       <  1:1481263/150‑1 (MQ=255)
                                                                                                aaCCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAAc                                      <  1:2282934/150‑1 (MQ=255)
                                                                                                 aCCAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACt                                     >  1:4065487/1‑150 (MQ=255)
                                                                                                   cAGGACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTgg                                   <  1:1443925/150‑1 (MQ=255)
                                                                                                      gACTCAGGAAGGAAACAATATTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTAAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTAt                                <  2:3525070/150‑1 (MQ=255)
                                                                                                      gACTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTAt                                >  2:1946830/1‑150 (MQ=255)
                                                                                                       aCTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATc                               >  1:1854802/1‑150 (MQ=255)
                                                                                                        cTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCa                              >  2:1349774/1‑150 (MQ=255)
                                                                                                        cTCAGGAAGGAAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCa                              >  2:4313939/1‑150 (MQ=255)
                                                                                                         tCAGGAAGCAAAAAATTTTGCTAAGCTTCATAATCCAAGTTTTAAGGAACGGCGTCCTCAAAAATTTACCCAAGAACAATTTCAATTTGCTTATGAGTTAATATAGCAAGGAATGATTTACAAGATGATTGAGCGAAAAGCTGGTATCAg                             <  2:4366510/150‑1 (MQ=255)
                                                                                                           aggaaggaAAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTa                           >  1:1809213/1‑150 (MQ=255)
                                                                                                            ggaaggaaAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTAt                          <  2:4565631/150‑1 (MQ=255)
                                                                                                            ggaaggaaAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTAt                          <  2:1615048/150‑1 (MQ=255)
                                                                                                             gaaggaaAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTAtt                         >  1:585878/1‑150 (MQ=255)
                                                                                                             gaaggaaAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATAGAGCGAAAAACTGGTATCAGTAtt                         <  2:1619709/150‑1 (MQ=255)
                                                                                                               aggaaAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGc                       >  2:698037/1‑150 (MQ=255)
                                                                                                                 gaaAAAATTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCgg                     <  1:2607073/150‑2 (MQ=255)
                                                                                                                  aaaaaaTTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCggc                    <  1:3303746/150‑3 (MQ=255)
                                                                                                                  aaaaaaTTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCggc                    >  1:1012662/1‑148 (MQ=255)
                                                                                                                  aaaaaaTTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCggc                    >  2:874758/1‑148 (MQ=255)
                                                                                                                   aaaaaTTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGGCt                   <  1:3751889/150‑1 (MQ=255)
                                                                                                                    aaaaTTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGGCTc                  <  2:2048686/150‑1 (MQ=255)
                                                                                                                    aaaaTTTTGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGGCTc                  <  2:639493/150‑1 (MQ=255)
                                                                                                                        ttttGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGGCTCaaaa              >  1:2891038/2‑150 (MQ=255)
                                                                                                                        ttttGCTAAGCTTCATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATAAAAAAAAAAAAAACTGGTATCAGTATTGCGGCTCaaaa              <  2:2960449/149‑1 (MQ=255)
                                                                                                                                agcttcATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGGCTCAAAAACGAAGAt      <  2:3271551/145‑1 (MQ=255)
                                                                                                                                 gcttcATGATCCAAGTTTTAAGGAAGGGCGTCCTGAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGGCTCAAAAACGAAGAtg     >  2:329524/5‑149 (MQ=255)
                                                                                                                                   ttcATGATCCAAGTTTTAAGGAAGGGCGTCCTCAAAAATTTACCGAAGAACAAATTCAATTTGCTTATGAGTTAAAACAGCAAGGAATGACTTACAAGATGATTGAGCGAAAAACTGATATCAGTATTGCGGCTCAAAAACGAAGATTTa   >  2:3070238/3‑150 (MQ=255)
                                                                                                                                               ||                                                                                                                                         
TATATTAAATATTGGACTTATCGATAATACACCGACAGGTCAACTTATTTTTACCATCTTCAGTGCTTTTGCGCAGTTTGAACGAGACCTTATTGTAACCAGGACTCAGGAAGGAAAAAACTTTGCTAAAATCCATGATCCGAACTTTAAAGAAGGACGTCCTCAGAAATTTACTGAAGAACAAATTCAATTTGCTTATGAATTAAAGCAGCAAGGTATGACTTACAAGATGATTGAGCGAAAAACTGGTATCAGTATTGCGACTCAAAAAAGAAGATTTAT  >  CP043523/1867788‑1868069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑