BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,848,290	+AT	intergenic (+275/‑2)	FTN78_chr1897 → / → FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,848,286	1	.	A	98.0%	353.9 / 7.4	205	intergenic (+271/‑6)	FTN78_chr1897/FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base . (2/2); new base A (135/64); total (137/68)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*	CP043523	1,848,286	2	.	T	97.1%	355.0 / 14.5	205	intergenic (+271/‑6)	FTN78_chr1897/FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base . (2/4); new base T (135/64); total (137/68)
Fisher's exact test for biased strand distribution p-value = 9.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

TACGATATGGATTTTATTAAT‑GGAGAGTAGTTTGACATTGACTATAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATAA‑‑A‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCT  >  CP043523/1848141‑1848382

||

tACGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑a                                                                                                     <  2:1484411/148‑1 (MQ=17)

tACGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑a                                                                                                     >  1:2745098/1‑148 (MQ=17)

tACGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑a                                                                                                     >  2:357163/1‑148 (MQ=17)

 aCGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑a‑‑t                                                                                                  <  2:468085/148‑1 (MQ=17)

 aCGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑a‑‑t                                                                                                  <  2:3753172/148‑1 (MQ=17)

 aCGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑a‑‑t                                                                                                  >  2:392069/1‑148 (MQ=17)

  cGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAaataa‑‑a‑‑ta                                                                                                 >  2:3330441/1‑148 (MQ=17)

  cGATATGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAaataa‑‑a‑‑ta                                                                                                 <  2:555279/148‑1 (MQ=17)

       tGGATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tatta                                                                                          >  1:1182454/1‑144 (MQ=14)

        ggATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattat                                                                                         >  1:739166/1‑143 (MQ=14)

        ggATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattat                                                                                         >  2:4502508/1‑143 (MQ=14)

        ggATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattat                                                                                         >  2:4600159/1‑143 (MQ=14)

        ggATTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattat                                                                                         >  2:2207293/1‑143 (MQ=14)

          aTTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattatgg                                                                                       >  2:1420821/1‑141 (MQ=14)

          aTTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattatgg                                                                                       >  2:4103538/1‑141 (MQ=14)

          aTTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattatgg                                                                                       <  1:807280/148‑8 (MQ=14)

          aTTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattatgg                                                                                       <  2:1548380/148‑8 (MQ=14)

          aTTTTATTAATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑a‑‑ta‑‑tattatgg                                                                                       >  1:3041848/1‑141 (MQ=14)

             ttattaATCAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCaa                                                                                      <  2:2963251/148‑1 (MQ=14)

                ttaatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATg                                                                                   >  2:265882/8‑148 (MQ=14)

                 taatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGGTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATgg                                                                                  <  2:2392199/142‑1 (MQ=14)

                 taatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGTCAAATgg                                                                                  >  1:1930596/7‑148 (MQ=14)

                  aatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGa                                                                                 >  1:109000/6‑148 (MQ=14)

                  aatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGa                                                                                 <  2:2769976/143‑1 (MQ=14)

                  aatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGa                                                                                 <  1:2070860/143‑1 (MQ=14)

                    tcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAAc                                                                               >  1:2142237/4‑148 (MQ=14)

                    tcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAAc                                                                               >  2:4433567/4‑148 (MQ=14)

                      agagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAg                                                                             <  2:4316431/147‑1 (MQ=14)

                      agagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAg                                                                             >  1:4688728/2‑148 (MQ=14)

                       gagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGt                                                                            >  2:873850/1‑148 (MQ=14)

                        agagTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTa                                                                           >  2:4535398/1‑148 (MQ=14)

                         gagTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTaa                                                                          >  2:1136355/1‑148 (MQ=11)

                            tagtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAAt                                                                       >  2:1900431/1‑148 (MQ=11)

                            tagtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAAt                                                                       >  1:975079/1‑148 (MQ=11)

                            tagtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAAt                                                                       >  2:1256459/1‑148 (MQ=11)

                              gtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATgg                                                                     <  2:2028359/148‑1 (MQ=11)

                              gtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATgg                                                                     >  1:4332623/1‑148 (MQ=11)

                              gtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATgg                                                                     >  1:2487487/1‑148 (MQ=11)

                               tttGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGt                                                                    >  1:2470363/1‑148 (MQ=11)

                               tttGAAATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGt                                                                    <  2:2892038/148‑1 (MQ=11)

                                 tGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGttt                                                                  >  2:1406057/1‑148 (MQ=11)

                                  gACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTa                                                                 >  2:4291649/1‑148 (MQ=11)

                                    cATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAAc                                                               >  2:799217/1‑148 (MQ=11)

                                       tgagtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGct                                                            >  2:4636211/5‑147 (MQ=11)

                                       tGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGct                                                            >  2:3504126/1‑147 (MQ=11)

                                        gagtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGcta                                                           >  1:1995174/4‑146 (MQ=11)

                                         agtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTATCGctac                                                          <  2:1332201/146‑4 (MQ=11)

                                         agtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGctac                                                          >  1:2202316/3‑145 (MQ=11)

                                          gtataATGAGTATTATGATAAAGAAGAAGATTTTGTCATGAGTAAAATCTTTTATAGAATCACTTTTCGCAAAATAAGATTTAACAATTTGTCGAAAGGAAATAA‑‑ATCTA‑‑TAATAGCGAATGGAACCGTAAACTGGTTTAACGctact                                                         <  2:2656327/147‑5 (MQ=2)

                                            ataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGctactaa                                                       >  2:1706789/1‑142 (MQ=14)

                                             taATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGctactaaa                                                      >  1:3378454/1‑141 (MQ=14)

                                             taATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGctactaaa                                                      <  2:2943503/148‑8 (MQ=11)

                                                  aGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                 >  2:2792447/1‑148 (MQ=11)

                                                   gtgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAttt                                                >  1:398047/1‑148 (MQ=14)

                                                    tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               <  2:35883/148‑1 (MQ=11)

                                                    tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               >  1:532317/1‑148 (MQ=11)

                                                    tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               <  1:846853/148‑1 (MQ=11)

                                                      ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                             <  2:2115045/148‑2 (MQ=11)

                                                       tattatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggtt                                            <  2:2749257/148‑3 (MQ=11)

                                                         ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          >  2:20720/1‑148 (MQ=11)

                                                         ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          <  1:4257867/148‑1 (MQ=11)

                                                           ataaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAt                                        <  2:3692140/145‑1 (MQ=11)

                                                           ataaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAt                                        <  2:3222903/145‑1 (MQ=11)

                                                             aaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAtta                                      >  1:1963178/2‑146 (MQ=11)

                                                              aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttac                                     >  1:2420193/1‑145 (MQ=11)

                                                               agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacc                                    >  2:1780777/1‑144 (MQ=11)

                                                                gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  2:3628939/1‑143 (MQ=11)

                                                                gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   <  1:1666976/148‑6 (MQ=11)

                                                                gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  1:582318/1‑143 (MQ=11)

                                                                gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAcct                                   >  2:1458577/1‑146 (MQ=11)

                                                                gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAcct                                   >  1:3769515/1‑146 (MQ=11)

                                                                    tagaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtga                               <  2:1190884/145‑1 (MQ=11)

                                                                       aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                            >  2:741771/1‑148 (MQ=11)

                                                                        ttttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagat                           <  1:1314774/148‑2 (MQ=11)

                                                                         tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  2:441915/1‑146 (MQ=11)

                                                                          ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATgg                         <  2:3107474/148‑1 (MQ=11)

                                                                           tGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggt                        <  2:4335786/148‑2 (MQ=11)

                                                                             tCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtca                      >  2:1621126/1‑142 (MQ=11)

                                                                              cATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                     >  1:4538907/1‑141 (MQ=11)

                                                                              cATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                     >  1:2790231/1‑141 (MQ=11)

                                                                               aTGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtcaag                    >  2:2432982/1‑143 (MQ=11)

                                                                                     aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGt              >  1:3510149/1‑148 (MQ=11)

                                                                                     aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGt              <  2:1286371/148‑1 (MQ=11)

                                                                                       aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  2:422389/1‑148 (MQ=11)

                                                                                       aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  1:3255539/1‑148 (MQ=11)

                                                                                        aTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCg           <  1:1600907/148‑1 (MQ=11)

                                                                                        aTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCg           >  1:404189/1‑148 (MQ=11)

                                                                                         tGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGc          >  2:3199024/1‑148 (MQ=11)

                                                                                         tGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGc          >  2:3737240/1‑148 (MQ=11)

                                                                                          gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  2:4580368/1‑148 (MQ=11)

                                                                                          gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  2:3424884/1‑148 (MQ=11)

                                                                                          gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  2:251760/1‑148 (MQ=11)

                                                                                           ttttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑A‑‑TATCTTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTc        >  2:1925682/1‑148 (MQ=11)

                                                                                             ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  1:3169294/1‑148 (MQ=11)

                                                                                             ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  2:2543720/1‑148 (MQ=11)

                                                                                              tataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACt     >  2:693467/1‑148 (MQ=11)

                                                                                               ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACtt    >  2:2833499/1‑148 (MQ=11)

                                                                                               ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACtt    >  1:2407566/1‑148 (MQ=11)

                                                                                                 aGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct  >  2:4331024/1‑148 (MQ=11)

                                                                                                 aGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATA‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct  >  1:1325423/1‑148 (MQ=11)

||

TACGATATGGATTTTATTAAT‑GGAGAGTAGTTTGACATTGACTATAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATAA‑‑A‑‑TA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCT  >  CP043523/1848141‑1848382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`