Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435232,059,9080GA55.7% 280.9 / inf 1062Y351Y (TAC→TAT) FTN78_chr2133D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (250/220);  new base A (290/301);  total (541/521)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 1065 total aligned reads displayed.

ATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAGTTC  >  CP043523/2059761‑2060055
                                                                                                                                                   |                                                                                                                                                   
 tttGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGt                                                                                                                                                    >  1:82118/1‑148 (MQ=255)
   tGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATaa                                                                                                                                                  >  1:3585753/1‑148 (MQ=255)
   tGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTaa                                                                                                                                                  <  2:156205/148‑1 (MQ=255)
    ggAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAg                                                                                                                                                 >  1:2476995/1‑148 (MQ=255)
       aGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaa                                                                                                                                              <  1:1703940/148‑1 (MQ=255)
        gATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaac                                                                                                                                             >  1:1621329/1‑148 (MQ=255)
        gATAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaac                                                                                                                                             >  1:366153/1‑148 (MQ=255)
        gATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGaac                                                                                                                                             >  1:1218270/1‑148 (MQ=255)
          tAGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGaacaa                                                                                                                                           <  2:2600997/148‑1 (MQ=255)
           aGCCTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGATTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTCGCATAAGGaacaac                                                                                                                                          >  2:1339445/1‑148 (MQ=255)
              cTAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACggg                                                                                                                                       >  2:1641016/1‑148 (MQ=255)
               tAAACTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGa                                                                                                                                      <  1:4645120/148‑1 (MQ=255)
                aaaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAg                                                                                                                                     >  2:80816/1‑148 (MQ=255)
                aaaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAg                                                                                                                                     >  2:3110001/1‑148 (MQ=255)
                aaaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTGTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTAGTGAAGTTTTCTGTCAGTGATTTTGTATCTGCAGTACCATGTGTAGCATAAGTAACGACggggg                                                                                                                                     >  1:4404599/1‑146 (MQ=255)
                 aaCTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGt                                                                                                                                    <  1:410519/148‑1 (MQ=255)
                   cTATCCTTTACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGa                                                                                                                                  <  2:2954220/148‑1 (MQ=255)
                    tATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGat                                                                                                                                 <  2:1151614/148‑1 (MQ=255)
                    tATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGat                                                                                                                                 >  1:1110098/1‑148 (MQ=255)
                          ttACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTtg                                                                                                                           >  1:4542776/1‑148 (MQ=255)
                           tACTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTtgt                                                                                                                          <  1:291803/148‑1 (MQ=255)
                            aCTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTtgtg                                                                                                                         <  1:88804/148‑1 (MQ=255)
                             cTGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTgg                                                                                                                        <  1:181590/148‑1 (MQ=255)
                              tGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGa                                                                                                                       >  2:4586757/1‑148 (MQ=255)
                              tGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGa                                                                                                                       >  1:2653623/1‑148 (MQ=255)
                              tGTAGTAGAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGa                                                                                                                       <  1:2536304/148‑1 (MQ=255)
                                tagtagAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGc                                                                                                                     <  2:73362/148‑1 (MQ=255)
                                 agtagAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCa                                                                                                                    >  2:2385737/1‑148 (MQ=255)
                                 agtagAAAGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCa                                                                                                                    <  2:4682102/148‑1 (MQ=255)
                                   tggAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAAc                                                                                                                  <  2:2528093/146‑1 (MQ=255)
                                   tagAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAAc                                                                                                                  <  2:2674664/148‑1 (MQ=255)
                                   tagAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAAc                                                                                                                  >  1:384669/1‑148 (MQ=255)
                                      aaaGTGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAg                                                                                                               <  1:3288981/148‑1 (MQ=255)
                                       aagtgTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGt                                                                                                              <  2:2047681/144‑1 (MQ=255)
                                         gagTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTg                                                                                                            <  1:2076011/148‑1 (MQ=255)
                                            tGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAAc                                                                                                         <  1:2124034/148‑1 (MQ=255)
                                                 aGTTTTTGGTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGAtt                                                                                                    >  1:2984166/1‑148 (MQ=255)
                                                     tttttttttttttCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTTTATCTGCACTACCATCTTTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttc                                                                                                >  2:277082/6‑148 (MQ=255)
                                                     tttttttttttttCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACTATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttc                                                                                                >  2:3382735/6‑148 (MQ=255)
                                                     tttGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttc                                                                                                >  1:231151/1‑148 (MQ=255)
                                                     tttGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttc                                                                                                >  2:4109325/1‑148 (MQ=255)
                                                        ggTTTTTAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttcttt                                                                                             <  2:1947850/148‑1 (MQ=255)
                                                         gTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATttttctttt                                                                                            >  1:1895120/1‑148 (MQ=255)
                                                             ttAGTCACAGTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCat                                                                                        <  1:742497/148‑1 (MQ=255)
                                                               aGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatca                                                                                      >  2:2251854/1‑148 (MQ=255)
                                                               aGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatca                                                                                      <  2:4164143/148‑1 (MQ=255)
                                                                  cacaGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCatcaatc                                                                                   <  2:630304/148‑1 (MQ=255)
                                                                     aGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATcaca                                                                                >  1:3759076/1‑148 (MQ=255)
                                                                      gTTGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAg                                                                               >  2:4424727/1‑148 (MQ=255)
                                                                        tGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAgca                                                                             >  2:3513348/1‑146 (MQ=255)
                                                                        tGCTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAgca                                                                             <  2:1243668/148‑3 (MQ=255)
                                                                        tGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTa                                                                             <  1:1290911/148‑1 (MQ=255)
                                                                         gCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTaa                                                                            >  1:939464/1‑148 (MQ=255)
                                                                          cTTCAACTGAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAAt                                                                           <  2:4016218/148‑1 (MQ=255)
                                                                             cAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTc                                                                        >  1:1559742/1‑148 (MQ=255)
                                                                                ctgAGGCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCttt                                                                     <  2:288086/146‑1 (MQ=255)
                                                                                    ggCTGTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTaaaa                                                                 <  2:232807/148‑1 (MQ=255)
                                                                                      cTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAatc                                                               >  1:3459741/1‑148 (MQ=255)
                                                                                        gTTTTTTTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAatcat                                                             >  2:1784927/1‑148 (MQ=255)
                                                                                        gTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAatcat                                                             <  2:4345072/148‑1 (MQ=255)
                                                                                           ttttTCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTa                                                          <  1:54789/148‑1 (MQ=255)
                                                                                            ttttcTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTaa                                                         >  2:905226/5‑148 (MQ=255)
                                                                                            ttttCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCCTCAGTCACGGCAATTTCTTTAAAATCATCCaa                                                         >  2:3678840/1‑148 (MQ=255)
                                                                                              ttcTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAgg                                                       >  2:2101517/3‑148 (MQ=255)
                                                                                               tcTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGga                                                      >  2:2423155/2‑148 (MQ=255)
                                                                                                cTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGgat                                                     >  2:527580/1‑148 (MQ=255)
                                                                                                 ttACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGgatt                                                    <  2:3028277/148‑1 (MQ=255)
                                                                                                  tACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGgattt                                                   >  1:2412768/1‑147 (MQ=255)
                                                                                                   tcTGCATTTGACTGTCAGTGATTTTGTACCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGgattta                                                  <  1:1013702/147‑3 (MQ=255)
                                                                                                     tGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGgatttatt                                                >  1:1351066/1‑148 (MQ=255)
                                                                                                      gAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGatttattt                                               <  2:282979/148‑1 (MQ=255)
                                                                                                       aaTTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGAtttt                                              <  2:1385546/148‑1 (MQ=255)
                                                                                                       aaTTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGAtttt                                              >  1:3396010/1‑148 (MQ=255)
                                                                                                        aTTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTAttttt                                             >  2:3896389/1‑148 (MQ=255)
                                                                                                         tttGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTAtttttt                                            <  1:3912163/148‑1 (MQ=255)
                                                                                                            gACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGc                                         <  1:287080/148‑1 (MQ=255)
                                                                                                               tGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGcaca                                      <  2:1084606/148‑1 (MQ=255)
                                                                                                                gTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACaa                                     >  2:3165069/1‑148 (MQ=255)
                                                                                                                     tGATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTg                                >  1:3109053/1‑148 (MQ=255)
                                                                                                                      gATTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTgg                               <  2:3292717/148‑1 (MQ=255)
                                                                                                                       aTTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGt                              >  1:1463851/1‑148 (MQ=255)
                                                                                                                       aTTTTGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGt                              >  2:220902/1‑148 (MQ=255)
                                                                                                                          ttGTATCTGCACTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGt                           <  1:2202710/148‑1 (MQ=255)
                                                                                                                           tGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTa                          >  1:4040880/1‑148 (MQ=255)
                                                                                                                            gTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTAc                         <  1:4697373/148‑1 (MQ=255)
                                                                                                                                    aCTACCATCTGTAGCGTAAGGAACACCGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGa                 <  2:3233198/148‑1 (MQ=255)
                                                                                                                                     cTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGAc                >  1:660989/1‑148 (MQ=255)
                                                                                                                                     cTACCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGAc                >  2:1886145/1‑148 (MQ=255)
                                                                                                                                      tACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGAcc               <  2:3419776/148‑1 (MQ=255)
                                                                                                                                       aCCATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCa              >  1:2842990/1‑148 (MQ=255)
                                                                                                                                         cATCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATa            <  1:4585689/148‑1 (MQ=255)
                                                                                                                                           tCTGTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATaaa          >  2:3603230/1‑148 (MQ=255)
                                                                                                                                             tGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATGAACa        <  2:4501494/148‑1 (MQ=255)
                                                                                                                                             tGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACa        <  1:4300543/148‑1 (MQ=255)
                                                                                                                                              gTAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACAt       >  2:4255847/1‑148 (MQ=255)
                                                                                                                                               tAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATa      >  2:3779785/1‑148 (MQ=255)
                                                                                                                                               tAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATa      >  1:422036/1‑148 (MQ=255)
                                                                                                                                               tAGCATAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAGTCACAGCAATTTCTTTAAAATCATCTAAGGATTTATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATa      <  1:2767243/148‑1 (MQ=255)
                                                                                                                                                  cGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTTTACAATACTTGTCGTACGCCAATGACCATAAACATAGtt   >  2:1037537/1‑148 (MQ=255)
                                                                                                                                                   gTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAGTtc  >  1:3192612/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATTTGGAAGATAGCCTAAACTATCCTTTACTGTAGTAGAAAGAGTGACAAGTTTTTGGTTTTTAGTCACAGTTGCTTCAACGGAGGCTGTTTTTTCCTTACTGAATTTGACTGTCAGTGATTTTGTATCTGCACTACCATCTGTAGCGTAAGGAACAACGGGAGTGATATTTTGTGGAGCAACGAGTTGAACAGATTTTTCTTTTCCATCAATCACAGTAATTTCTTTAAAATCATCTAAGGATTGATTTTTTTGCACAAGACTGGTCGTACGCCAATGACCATAAACATAGTTC  >  CP043523/2059761‑2060055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑