Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,862,0660GA64.9% 9.9 / 32.1 37L38L (CTC→CTT) FTN78_chr1910hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (5/8);  new base A (15/9);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAAGTTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAGCTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGTTTA  >  CP043523/1861979‑1862213
                                                                                       |                                                                                                                                                   
aGCGAGTTGGTGCTTAGCTTTAAGCGTGTCGCTAAACTGCAGTACGCCCCATAGGTAACAAGCGATACCTAAGCAATCTGATGTTGCGAGATGGACGTTGTTTCGGGTTTGGGCCTCACGGGAAACACTCGTTTGATAAAGCGGCTCa                                                                                         <  2:4035912/148‑1 (MQ=11)
            cTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAGCTAAACAGTCAGATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGt                                                                             <  2:1430029/148‑1 (MQ=11)
                                                                        acagtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTa                 <  2:3397696/141‑1 (MQ=0)
                                                                        acagtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTa                 >  2:4256173/8‑148 (MQ=1)
                                                                         tagtcagATGTTGCAAGGTTGACGTTCTATCTGTTTTGAGCCTCAAGGGGAACACTGGTTTGATACAGCGCCTCAGTCGTTGGAAGTAAATAAACAAACACTGTTGGAAGTGTGATCTTATAAGTCATATAAGTCGTGAGCTTTCTaa                <  2:1869538/142‑1 (MQ=0)
                                                                         cagtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTaa                <  2:3086858/142‑1 (MQ=0)
                                                                          agtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAATCGCTTCAATGCTTGTCAGTAAATAAACAAAAACTTTTGTAAGCGTGCTATTATAAGTCATATAGGTCGTGCGCTTTCTAAt               <  2:4417815/143‑1 (MQ=1)
                                                                          agtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAAt               >  1:1193596/6‑148 (MQ=0)
                                                                          agtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAAt               <  1:3587124/143‑1 (MQ=0)
                                                                           gtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATg              <  1:1107716/144‑1 (MQ=0)
                                                                           gtcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTATCAATGGTTGTCAGTAAATAAGCAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATg              <  1:1787410/144‑1 (MQ=1)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAATCGCTTCAATGCTTGTCAGTAAATAAACAAAAACTTTTGTAAGCGTGCTATTATAAGTCATATAGGTCGTGCGCTTTCTAATGc             >  1:528177/4‑148 (MQ=1)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGc             >  2:833088/4‑148 (MQ=1)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:3365073/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:4399210/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  1:303219/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  1:1308232/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  1:3853941/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:839363/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:1022256/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:838923/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:1201914/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:1202019/4‑148 (MQ=0)
                                                                            tcagATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACGCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGc             >  2:3412380/4‑148 (MQ=0)
                                                                                    tGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGt     <  1:1852646/148‑1 (MQ=2)
                                                                                    tGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGt     >  2:3325868/1‑148 (MQ=2)
                                                                                    tGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGt     <  2:2123042/148‑1 (MQ=11)
                                                                                    tGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAATCGCTTCAATGCTTGTCAGTAAATAAACAAAAACTTTTGTAAGCGTGCTATTATAAGTCATATAGGTCGTGCGCTTTCTAATGCTTAGTGGt     <  2:1062823/148‑1 (MQ=11)
                                                                                     gCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGtt    <  1:2265214/148‑1 (MQ=9)
                                                                                     gCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGtt    <  2:1103101/148‑1 (MQ=9)
                                                                                     gCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGtt    <  2:493745/148‑1 (MQ=9)
                                                                                     gCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGtt    >  1:2445064/1‑148 (MQ=14)
                                                                                     gCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGtt    >  1:1913173/1‑148 (MQ=14)
                                                                                      cGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGttt   <  1:1856558/148‑1 (MQ=12)
                                                                                      cGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGttt   >  1:1624316/1‑148 (MQ=17)
                                                                                      cGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGttt   >  1:1620709/1‑148 (MQ=12)
                                                                                      cGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGttt   <  2:1459797/148‑1 (MQ=12)
                                                                                       gagaTGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGTTTa  <  1:769080/148‑1 (MQ=17)
                                                                                       gagaTGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGTTTa  <  1:3677918/148‑1 (MQ=12)
                                                                                       gagaTGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGTTTa  <  2:956073/148‑1 (MQ=12)
                                                                                       gagaTGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGTTTa  <  1:3447692/148‑1 (MQ=12)
                                                                                       gagaTGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGTGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGTTTTCTAATGCTTAGTGGTTTa  <  1:2667115/148‑1 (MQ=12)
                                                                                       gagaTAGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACCCTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGGAGTGTGCTATTATAAGTCATATAAGTCGTGAGCTTTCTAATGCTTAGTGCTTTa  <  2:3633234/148‑1 (MQ=11)
                                                                                       |                                                                                                                                                   
AGCAAGTTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAGCTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGGAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAAAACTTTTGGAAGTGTGCTATTATAAGTCATATAAGTCGTGCGCTTTCTAATGCTTAGTGGTTTA  >  CP043523/1861979‑1862213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑