BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,848,257	G→A	intergenic (+242/‑35)	FTN78_chr1897 → / → FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,848,257	0	G	A	98.8%	656.1 / 0.1	339	intergenic (+242/‑35)	FTN78_chr1897/FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base G (4/0); new base A (216/118); total (221/118)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 347 total aligned reads displayed.

TCCCTACAGTTCATAAAATCCATGTCATTACGATATGGATTTTATTAAT‑G‑GAGAGTAGTTTGACATTGACTATAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑A‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCT  >  CP043523/1848113‑1848382

 ccttACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATaaga                                                                                                                                 <  2:3430898/145‑1 (MQ=18)

 cccTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATaaga                                                                                                                                 <  2:3035187/148‑1 (MQ=18)

  ccTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAt                                                                                                                                >  2:3104144/1‑148 (MQ=18)

    tACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAttt                                                                                                                              >  2:187820/1‑148 (MQ=18)

    tACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAttt                                                                                                                              <  2:4684214/148‑1 (MQ=18)

    tACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAttt                                                                                                                              <  1:2408531/148‑1 (MQ=18)

     aCAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTa                                                                                                                             >  1:4609902/1‑148 (MQ=18)

       aGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACa                                                                                                                           >  2:3708549/1‑148 (MQ=18)

        gTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACaa                                                                                                                          >  1:2422353/1‑148 (MQ=18)

          tCATAAAATCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAAtt                                                                                                                        <  2:649419/148‑1 (MQ=18)

              aaaaTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGt                                                                                                                    >  1:2535211/1‑148 (MQ=18)

               aaaTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTc                                                                                                                   >  1:1350262/1‑148 (MQ=18)

                aaTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCg                                                                                                                  <  2:1010508/148‑1 (MQ=18)

                 aTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGa                                                                                                                 >  2:1572679/1‑148 (MQ=17)

                 aTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGa                                                                                                                 >  1:2880597/1‑148 (MQ=18)

                 aTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGa                                                                                                                 <  1:1057960/148‑1 (MQ=18)

                 aTCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGa                                                                                                                 <  2:1803179/148‑1 (MQ=18)

                  tCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACCTTTCGCAAAATCAGATTTACCATTTTGTCGaa                                                                                                                >  2:4099473/1‑148 (MQ=17)

                   ccATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGaaa                                                                                                               >  2:3249596/1‑148 (MQ=17)

                    cATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGaaaa                                                                                                              >  2:1769020/1‑147 (MQ=17)

                    cATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAg                                                                                                              >  2:3201350/1‑148 (MQ=17)

                     aTGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAgg                                                                                                             <  2:1701119/148‑1 (MQ=17)

                      tGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGa                                                                                                            >  1:1156916/1‑148 (MQ=17)

                      tGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGa                                                                                                            >  2:124690/1‑148 (MQ=17)

                      tGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGa                                                                                                            >  1:3394529/1‑148 (MQ=17)

                      tGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTATCGAAAGGa                                                                                                            >  2:3915709/1‑148 (MQ=17)

                       gTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaa                                                                                                           >  2:2467354/1‑148 (MQ=17)

                        tcactACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaa                                                                                                          >  2:4272348/5‑148 (MQ=17)

                         cactACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaat                                                                                                         >  1:1619926/4‑148 (MQ=17)

                           ctACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaata‑a                                                                                                      >  2:3418896/2‑148 (MQ=17)

                            tACGATATGGATTTTATTAATCA‑GAGCGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaata‑a‑‑a                                                                                                   >  2:4593783/1‑148 (MQ=17)

                             aCGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaata‑a‑‑a‑‑t                                                                                                >  1:1529357/1‑148 (MQ=17)

                              cGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAaata‑a‑‑a‑‑ta                                                                                               <  2:555279/148‑1 (MQ=17)

                               gATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑a‑‑tat                                                                                              >  1:645143/1‑148 (MQ=17)

                                  atGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑a‑‑tatatt                                                                                           <  2:2239361/148‑4 (MQ=14)

                                   tGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑a‑‑tatatta                                                                                          >  1:3486410/1‑144 (MQ=14)

                                    ggATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑a‑‑tatattat                                                                                         >  2:4502506/1‑143 (MQ=14)

                                      aTTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑a‑‑tatattatgg                                                                                       <  1:807280/148‑8 (MQ=14)

                                      aTTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑a‑‑tatattatgg                                                                                       >  2:1420821/1‑141 (MQ=14)

                                              taatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGGTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATgg                                                                                  <  2:2392199/142‑1 (MQ=14)

                                               aatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGa                                                                                 <  2:2769592/143‑1 (MQ=14)

                                               aatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGa                                                                                 <  1:2070860/143‑1 (MQ=14)

                                                 tcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAAc                                                                               >  1:1954656/4‑148 (MQ=14)

                                                  cagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACa                                                                              <  2:1182454/146‑1 (MQ=14)

                                                   agagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAg                                                                             >  1:4688728/2‑148 (MQ=14)

                                                     agagTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTa                                                                           <  2:2689550/148‑1 (MQ=14)

                                                     agagTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTa                                                                           >  2:4535993/1‑148 (MQ=14)

                                                       agtagtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTaaa                                                                         >  2:3750424/1‑148 (MQ=11)

                                                          agtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATg                                                                      <  2:87161/148‑1 (MQ=11)

                                                           gtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATgg                                                                     >  1:2487487/1‑148 (MQ=11)

                                                           gtTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATgg                                                                     >  1:4332623/1‑148 (MQ=11)

                                                            tttGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGt                                                                    >  1:2470363/1‑148 (MQ=11)

                                                            tttGAAATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGt                                                                    <  2:2892038/148‑1 (MQ=11)

                                                             ttGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGtt                                                                   <  2:1900946/148‑1 (MQ=11)

                                                              tGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGttt                                                                  >  2:1406057/1‑148 (MQ=11)

                                                               gACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTa                                                                 >  2:4291649/1‑148 (MQ=11)

                                                                 cATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGGAAAATGGTTTAAc                                                               >  1:4120615/1‑148 (MQ=11)

                                                                    tgagtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGct                                                            >  2:4636211/5‑147 (MQ=11)

                                                                    tGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGct                                                            <  2:1485633/148‑2 (MQ=11)

                                                                     gagtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGcta                                                           >  1:2317350/4‑146 (MQ=11)

                                                                      agtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTATCGctac                                                          <  2:1332201/146‑4 (MQ=11)

                                                                      agtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGctac                                                          >  1:2202260/3‑145 (MQ=11)

                                                                       gtataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGctact                                                         <  2:3576963/147‑5 (MQ=11)

                                                                       gtataATGAGTATTATGATAAAGAAGAAGATTTTGTCATGAGTAAAATCTTTTATAGAATCACTTTTCGCAAAATAAGATTTAACAATTTGTCGAAAGGAAATA‑A‑‑ATCTATAATAGCGAATGGAACCGTAAACTGGTTTAACGctact                                                         <  2:2656327/147‑5 (MQ=2)

                                                                         ataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGctactaa                                                       >  2:1711244/1‑142 (MQ=11)

                                                                         ataATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGctactaa                                                       <  2:2844748/148‑7 (MQ=14)

                                                                          taATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGctactaaa                                                      <  2:2951546/148‑8 (MQ=11)

                                                                               aGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                 >  2:2791848/1‑148 (MQ=11)

                                                                                gtgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAttt                                                >  1:398047/1‑148 (MQ=14)

                                                                                 tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               <  1:846853/148‑1 (MQ=11)

                                                                                   ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                             <  2:2115045/148‑2 (MQ=11)

                                                                                     attatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                           <  2:4431737/148‑1 (MQ=11)

                                                                                     attatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                           >  1:533871/1‑148 (MQ=11)

                                                                                      ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          >  2:20720/1‑148 (MQ=11)

                                                                                      ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          >  2:3992256/1‑148 (MQ=11)

                                                                                           aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAc                                     >  2:747105/1‑148 (MQ=11)

                                                                                            agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacc                                    >  2:1639258/1‑144 (MQ=11)

                                                                                            agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacc                                    >  2:70120/1‑144 (MQ=11)

                                                                                             gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   <  1:1666976/148‑6 (MQ=11)

                                                                                             gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  2:3629304/1‑143 (MQ=11)

                                                                                             gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  2:1458896/1‑143 (MQ=11)

                                                                                             gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAcct                                   >  1:3769515/1‑146 (MQ=11)

                                                                                                    aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                            <  2:252845/148‑1 (MQ=11)

                                                                                                      tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  2:2098484/1‑146 (MQ=11)

                                                                                                      tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  1:2096044/1‑146 (MQ=11)

                                                                                                       ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATgg                         <  2:3108603/148‑1 (MQ=11)

                                                                                                        tGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggt                        <  2:4335786/148‑2 (MQ=11)

                                                                                                           cATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtcaa                     >  2:3532205/1‑144 (MQ=11)

                                                                                                           cATGAGTAAAATGTTTTATAGAATCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtcaa                     <  2:3057057/148‑5 (MQ=11)

                                                                                                                  aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGt              <  2:1286371/148‑1 (MQ=11)

                                                                                                                  aaaaTGTGATATAGATTCACTTTTCGCAAAATAAGATTTACTATTTTGTCGAAAGGAGATACAT‑A‑‑TATTATGGCACATGGACCCGTAAAATGGTTTAACGCTACTAAAGGATTTGGTGTTATTACCTCTGAAGATGGTCAAGATTTGt              <  2:1936423/148‑1 (MQ=2)

                                                                                                                    aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  2:422389/1‑148 (MQ=11)

                                                                                                                    aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  1:3255539/1‑148 (MQ=11)

                                                                                                                    aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            <  2:2053657/148‑1 (MQ=11)

                                                                                                                       gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  1:1989805/1‑148 (MQ=11)

                                                                                                                          ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  1:276299/1‑148 (MQ=11)

                                                                                                                          ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  1:3169294/1‑148 (MQ=11)

                                                                                                                          ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  2:898112/1‑148 (MQ=11)

                                                                                                                           tataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACt     >  1:1296766/1‑148 (MQ=11)

                                                                                                                              aGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATA‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct  >  2:4330350/1‑148 (MQ=11)

TCCCTACAGTTCATAAAATCCATGTCATTACGATATGGATTTTATTAAT‑G‑GAGAGTAGTTTGACATTGACTATAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑A‑‑TATTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCT  >  CP043523/1848113‑1848382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`