Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,470 A→G E384E (GAA→GAG)  FTN78_chr1930 → Putative UV‑damage repair protein UvrX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,4700AG99.6% 1679.1 / ‑4.2 479E384E (GAA→GAG) FTN78_chr1930Putative UV‑damage repair protein UvrX
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (224/252);  total (224/255)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 479 total aligned reads displayed.

AAATGGCTGAGAACTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGAGCTGCTTCTACTTCTGAATACTCATCTATTAAGGTTTCTCGCAATATCGAAGCAACGCAAAACACAAAGGAATTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATTAGACAAATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAAT  >  CP043523/1885325‑1885614
                                                                                                                                                 |                                                                                                                                                
  aTGGCTGAGAACTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGc                                                                                                                                              >  2:3532376/1‑148 (MQ=37)
  aTGGCTGAGAACTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGc                                                                                                                                              >  1:1612981/1‑148 (MQ=37)
        gagaACTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGAcc                                                                                                                                        <  2:760716/148‑1 (MQ=37)
         agaACTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCt                                                                                                                                       >  2:2764190/1‑148 (MQ=37)
          gaACTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTc                                                                                                                                      <  1:344032/148‑1 (MQ=37)
            aCTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGc                                                                                                                                    <  1:1143786/148‑1 (MQ=37)
            aCTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGc                                                                                                                                    >  1:4008869/1‑148 (MQ=37)
                 gCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAAtct                                                                                                                               >  1:59747/1‑148 (MQ=25)
                  cGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAAtctc                                                                                                                              >  2:2849668/1‑148 (MQ=37)
                  cGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAAtctc                                                                                                                              >  1:722241/1‑148 (MQ=37)
                   gATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATctct                                                                                                                             >  2:216294/1‑148 (MQ=25)
                   gATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATctct                                                                                                                             <  1:3906975/148‑1 (MQ=37)
                    aTAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCtt                                                                                                                            <  2:3614667/148‑1 (MQ=25)
                      aaGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTa                                                                                                                          >  1:954858/1‑148 (MQ=37)
                      aaGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTa                                                                                                                          >  1:532613/1‑148 (MQ=37)
                          ttGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTc                                                                                                                      <  1:1173184/148‑1 (MQ=37)
                             agaAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTg                                                                                                                   >  1:3039192/1‑148 (MQ=25)
                              gaAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGa                                                                                                                  >  1:3987073/1‑148 (MQ=25)
                               aaaGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGaa                                                                                                                 >  1:447080/1‑148 (MQ=25)
                                 aGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGaaaa                                                                                                               >  1:1858193/1‑148 (MQ=25)
                                   ggCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGt                                                                                                             <  1:1211801/148‑1 (MQ=25)
                                    gCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTa                                                                                                            >  2:2687976/1‑148 (MQ=25)
                                    gCGGAAAACTAGCAAGCAATCTTTCACTTTATACAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACAGAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTa                                                                                                            >  1:4221954/1‑148 (MQ=21)
                                        aaaaCTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCaa                                                                                                        <  1:1784126/148‑1 (MQ=25)
                                           aCTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAggg                                                                                                     >  2:3298714/1‑148 (MQ=25)
                                           aCTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAggg                                                                                                     <  1:470642/148‑1 (MQ=25)
                                            cTAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAgggg                                                                                                    <  1:4369525/148‑1 (MQ=25)
                                             tAGCAAGCAATCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAggggg                                                                                                   <  2:2618533/148‑1 (MQ=25)
                                                caagcaaTCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGc                                                                                                <  2:2046952/148‑1 (MQ=25)
                                                    caaTCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGAta                                                                                            <  2:2884386/148‑2 (MQ=255)
                                                    caaTCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGAta                                                                                            >  1:3850580/1‑147 (MQ=255)
                                                     aaTCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACTAAGGGGGAGCGAtaa                                                                                           <  1:1000365/148‑3 (MQ=255)
                                                     aaTCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGAtaa                                                                                           >  2:4106693/1‑146 (MQ=255)
                                                       tCTTTCACTTTATGCAGNTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGa                                                                                         >  2:4475453/1‑148 (MQ=255)
                                                       tCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGa                                                                                         >  1:2627621/1‑148 (MQ=255)
                                                       tCTTTCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGa                                                                                         >  2:1044030/1‑148 (MQ=255)
                                                         tttCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACa                                                                                       <  1:2882935/148‑1 (MQ=255)
                                                          ttCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTACGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACaa                                                                                      <  1:4267605/148‑1 (MQ=255)
                                                          ttCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACaa                                                                                      <  2:2310298/148‑1 (MQ=255)
                                                          ttCACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACaa                                                                                      >  2:382704/1‑148 (MQ=255)
                                                            cACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAAt                                                                                    >  2:4362188/1‑148 (MQ=255)
                                                            cACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAAt                                                                                    >  2:552988/1‑148 (MQ=255)
                                                            cACTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCCCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAAt                                                                                    >  1:2785631/1‑148 (MQ=255)
                                                              cTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGGCAAATAg                                                                                  >  1:3166070/1‑148 (MQ=255)
                                                              cTTTATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAg                                                                                  <  2:862794/148‑1 (MQ=255)
                                                                ttATGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAggg                                                                                <  1:1745571/148‑1 (MQ=255)
                                                                  aTGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGAt                                                                              >  2:1812295/1‑148 (MQ=255)
                                                                   tGCAGGTGCTGCTTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATa                                                                             >  2:778231/1‑148 (MQ=255)
                                                                         tgctgctTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCgga                                                                       <  2:392662/147‑2 (MQ=255)
                                                                            tgctTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAAt                                                                    <  2:3895472/148‑1 (MQ=255)
                                                                              ctTCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCa                                                                  <  2:2387240/148‑1 (MQ=255)
                                                                               ttCTACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCaa                                                                 <  2:4239009/148‑1 (MQ=255)
                                                                                  tACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACtt                                                              >  2:2028888/1‑148 (MQ=255)
                                                                                  tACTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACtt                                                              <  1:352884/148‑1 (MQ=255)
                                                                                   aCTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACttt                                                             <  1:238271/148‑1 (MQ=255)
                                                                                    cTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTc                                                            >  2:1524549/1‑148 (MQ=255)
                                                                                    cTTCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTc                                                            <  2:2528662/148‑1 (MQ=255)
                                                                                     ttCTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCt                                                           >  1:367281/1‑148 (MQ=255)
                                                                                       cTGAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGa                                                         <  1:3237550/148‑1 (MQ=255)
                                                                                         gAATACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATa                                                       >  1:1451575/1‑148 (MQ=255)
                                                                                           aTACTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATCGGGATAAGCGGAAATCAACTTTCTGATAGt                                                     >  1:4596434/1‑148 (MQ=255)
                                                                                             aCTCTTCTGTTAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTc                                                   >  2:1215347/1‑148 (MQ=255)
                                                                                                  tctgttAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTa                                              >  2:1839307/5‑148 (MQ=255)
                                                                                                  tatgttAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTa                                              >  2:3708901/5‑148 (MQ=255)
                                                                                                    tgttAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTaaa                                            >  1:4532018/3‑148 (MQ=255)
                                                                                                       tAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAaacaa                                         <  2:1325278/148‑1 (MQ=255)
                                                                                                       tAAGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAaacaa                                         >  1:1400220/1‑148 (MQ=255)
                                                                                                         aGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACt                                       <  2:3759571/148‑1 (MQ=255)
                                                                                                         aGGTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACt                                       >  1:1658114/1‑148 (MQ=255)
                                                                                                          ggTTTCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACtc                                      <  1:4139964/148‑2 (MQ=255)
                                                                                                             ttCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAActctct                                   >  1:4057818/1‑148 (MQ=255)
                                                                                                             ttCTCGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAActctct                                   <  2:4153469/148‑1 (MQ=255)
                                                                                                              tctcGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCtt                                  <  1:881454/148‑1 (MQ=255)
                                                                                                              tctcGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGCTAAACAACTCTCtt                                  <  2:1769988/148‑1 (MQ=255)
                                                                                                               ctcGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCttt                                 <  1:755842/148‑1 (MQ=255)
                                                                                                                 cGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTAt                               <  1:2010450/148‑1 (MQ=255)
                                                                                                                 cGCAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTAt                               <  2:654071/148‑1 (MQ=255)
                                                                                                                   cAATATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTAttt                             >  1:1507144/1‑148 (MQ=255)
                                                                                                                    aaTATTGAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTg                            >  1:2107416/1‑148 (MQ=255)
                                                                                                                       attgAAGCCACGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTCTCTGATAATTCGGTCAAACAACTTTCTTTATTTGaaa                         >  2:4544298/4‑148 (MQ=17)
                                                                                                                       agtggaGCCACGCAAAACACAAAGGAGTGGCAAGACCTCGCAATCTCTTTATCTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGaaa                         <  1:3622956/143‑1 (MQ=255)
                                                                                                                            agccaCGCAAAACACAAAGGCGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGtt                    <  2:1416876/144‑1 (MQ=255)
                                                                                                                            agccaCGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGtt                    >  2:1702302/5‑148 (MQ=255)
                                                                                                                                aCGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAg                >  1:2273568/1‑148 (MQ=255)
                                                                                                                                 cGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGa               >  2:2992006/1‑148 (MQ=255)
                                                                                                                                 cGCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGa               >  1:3336187/1‑148 (MQ=255)
                                                                                                                                  gCAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGaa              <  2:4707411/148‑1 (MQ=255)
                                                                                                                                   cAAAACACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGaaa             <  2:4008869/148‑1 (MQ=255)
                                                                                                                                    aaaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGaaaa            <  1:2491281/148‑1 (MQ=255)
                                                                                                                                    aaaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGaaaa            <  1:1362856/148‑1 (MQ=255)
                                                                                                                                     aaaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAAt           <  2:3101367/148‑1 (MQ=255)
                                                                                                                                      aaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATc          >  2:3409229/1‑148 (MQ=255)
                                                                                                                                      aaCACAAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATc          <  1:508298/148‑1 (MQ=255)
                                                                                                                                        cacaAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCaa        <  2:3046818/148‑1 (MQ=255)
                                                                                                                                        cacaAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCaa        >  2:948025/1‑148 (MQ=255)
                                                                                                                                          caAAGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAAc      <  1:2590659/148‑1 (MQ=255)
                                                                                                                                           aaaGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACt     <  1:3734940/148‑1 (MQ=255)
                                                                                                                                           aaaGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACt     >  2:4302083/1‑148 (MQ=255)
                                                                                                                                             aGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACtaa   >  2:72593/1‑148 (MQ=255)
                                                                                                                                             aGGAGTTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACtaa   >  2:3564892/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAATGGCTGAGAACTTAGCGATAAGATTGAGAAAGGGCGGAAAACTAGCAAGCAATCTTTCACTTTATGCAGGAGCTGCTTCTACTTCTGAATACTCATCTATTAAGGTTTCTCGCAATATCGAAGCAACGCAAAACACAAAGGAATTGCAAGACCTCGCAATCTCTTTATTTCGTGAAAAGTACCAAGGGGGAGCGATTAGACAAATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAAT  >  CP043523/1885325‑1885614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑