Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,662 A→T T448T (ACA→ACT)  FTN78_chr1930 → Putative UV‑damage repair protein UvrX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,6620AT99.8% 1505.2 / ‑4.7 501T448T (ACA→ACT) FTN78_chr1930Putative UV‑damage repair protein UvrX
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (281/219);  total (281/220)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 503 total aligned reads displayed.

GAGCGATTAGACAAATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGT  >  CP043523/1885517‑1885809
                                                                                                                                                 |                                                                                                                                                   
 aGCGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTc                                                                                                                                                  <  2:752231/148‑1 (MQ=255)
   cGATAAGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGa                                                                                                                                                >  2:2558600/1‑148 (MQ=255)
    gataaGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGAt                                                                                                                                               <  1:282644/144‑1 (MQ=255)
       aaGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGAtttt                                                                                                                                            >  1:2621552/2‑148 (MQ=255)
       aaGACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGAtttt                                                                                                                                            <  2:4225812/147‑1 (MQ=255)
         gACAAATAGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtct                                                                                                                                          <  1:2730667/148‑1 (MQ=255)
                aGGGATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtctttcaata                                                                                                                                   >  1:3525251/1‑141 (MQ=255)
                 gggATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATAc                                                                                                                                  >  1:2365489/1‑148 (MQ=255)
                  ggATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtcttt                                                                                                                                        >  2:2082921/1‑139 (MQ=255)
                  ggATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTtcttt                                                                                                                                        <  1:2082921/141‑3 (MQ=255)
                   gATAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaa                                                                                                                                >  1:2918383/1‑148 (MQ=255)
                   gATAAGCGGAAATCAACTTTAAGATAGTTATGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAAAAAAAAAAAAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaa                                                                                                                                <  2:3871889/148‑1 (MQ=255)
                     tAAGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaa                                                                                                                              <  2:283895/148‑1 (MQ=255)
                       aGCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACaaaagg                                                                                                                            >  2:3456635/1‑146 (MQ=255)
                        gCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGc                                                                                                                           >  2:4113844/1‑148 (MQ=255)
                        gCGGAAATCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGc                                                                                                                           <  1:2687976/148‑1 (MQ=255)
                           gaaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAag                                                                                                                        >  2:343141/3‑148 (MQ=255)
                            aaaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagt                                                                                                                       <  1:3809226/147‑1 (MQ=255)
                             aaTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagta                                                                                                                      <  2:4410771/148‑1 (MQ=255)
                              aTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagtag                                                                                                                     <  2:3039192/148‑1 (MQ=255)
                              aTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagtag                                                                                                                     >  2:1761469/1‑148 (MQ=255)
                              aTCAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAagtag                                                                                                                     >  1:4696175/1‑148 (MQ=255)
                                cAACTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGtt                                                                                                                   <  2:532613/148‑1 (MQ=255)
                                 aaCTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGttt                                                                                                                  <  2:4473732/148‑1 (MQ=255)
                                   cTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTAt                                                                                                                >  1:4344413/1‑148 (MQ=255)
                                   cTTTCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTAt                                                                                                                <  1:3665144/148‑1 (MQ=255)
                                     ttCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATcc                                                                                                              >  2:2633136/1‑148 (MQ=255)
                                      tCTGATAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCg                                                                                                             >  2:2304016/1‑148 (MQ=255)
                                          aTAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAgga                                                                                                         >  2:4601205/1‑147 (MQ=255)
                                           tAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggat                                                                                                        <  1:1417628/148‑3 (MQ=255)
                                           tAGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggat                                                                                                        >  1:2256967/1‑146 (MQ=255)
                                            aGTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggatt                                                                                                       <  2:3825368/148‑4 (MQ=255)
                                             gTTCTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattc                                                                                                      >  1:3030589/1‑144 (MQ=255)
                                                cTGTTAAACAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAggattcacg                                                                                                   >  2:3629849/1‑141 (MQ=255)
                                                     aaaCAACTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCa                                                                                              >  2:4681264/1‑148 (MQ=255)
                                                       acaacTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCAtt                                                                                            >  1:1750301/1‑148 (MQ=255)
                                                         aacTCTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTa                                                                                          >  2:35277/1‑148 (MQ=255)
                                                           ctctCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATc                                                                                        <  1:552988/145‑1 (MQ=255)
                                                            tctCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATGTATCg                                                                                       >  1:386892/3‑148 (MQ=255)
                                                             ctCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGACACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCgg                                                                                      >  1:1081229/2‑148 (MQ=255)
                                                               cTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGaa                                                                                    <  2:3170859/148‑1 (MQ=255)
                                                               cTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGaa                                                                                    >  2:241902/1‑148 (MQ=255)
                                                                 ttATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACa                                                                                  <  1:4427931/148‑1 (MQ=255)
                                                                 ttATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACa                                                                                  <  2:4057818/148‑1 (MQ=255)
                                                                 ttATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACa                                                                                  <  1:913581/148‑1 (MQ=255)
                                                                 ttATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACa                                                                                  <  2:1268182/148‑1 (MQ=255)
                                                                 ttATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACa                                                                                  <  2:1682554/148‑1 (MQ=255)
                                                                   aTTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACaaa                                                                                <  2:3955406/148‑1 (MQ=255)
                                                                   aTTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACaaa                                                                                <  1:709189/148‑1 (MQ=255)
                                                                      tGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTc                                                                             <  2:2123548/148‑1 (MQ=255)
                                                                       gAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCa                                                                            <  1:1696767/148‑1 (MQ=255)
                                                                        aaaGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCAt                                                                           >  1:1914182/1‑148 (MQ=255)
                                                                        aaaGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCAt                                                                           <  1:1503611/148‑1 (MQ=255)
                                                                        aaaGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCAt                                                                           <  2:595060/148‑1 (MQ=255)
                                                                         aaGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCAtt                                                                          >  1:2528444/1‑148 (MQ=255)
                                                                          aGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTg                                                                         >  2:1062907/1‑148 (MQ=255)
                                                                           gtgtTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTgg                                                                        <  1:4107997/148‑1 (MQ=255)
                                                                              ttCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAgg                                                                     <  1:4302083/148‑1 (MQ=255)
                                                                               tCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGt                                                                    >  1:4062288/1‑148 (MQ=255)
                                                                                cAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTc                                                                   <  2:3094836/148‑1 (MQ=255)
                                                                                  aGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCAt                                                                 >  1:2389290/1‑148 (MQ=255)
                                                                                      aaTCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATgcag                                                             >  1:450192/1‑148 (MQ=255)
                                                                                       aTCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATgcagc                                                            <  1:1162286/148‑1 (MQ=255)
                                                                                        tCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATgcagca                                                           >  2:4294214/1‑148 (MQ=255)
                                                                                         cAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCaa                                                          <  2:2935731/148‑1 (MQ=25)
                                                                                               aataaAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAg                                                    >  1:279826/1‑148 (MQ=25)
                                                                                                aaaaaaaGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCAGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGa                                                   >  2:1884460/3‑148 (MQ=21)
                                                                                                  aaaaaGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCATGAct                                                 >  1:1004880/1‑147 (MQ=21)
                                                                                                    aaaGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAag                                               >  2:449889/1‑148 (MQ=25)
                                                                                                     aaGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAagg                                              <  2:1997069/148‑1 (MQ=21)
                                                                                                         aaGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAaggaggt                                          >  1:4154782/1‑147 (MQ=25)
                                                                                                         aaGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAaggaggt                                          >  1:4632442/1‑147 (MQ=21)
                                                                                                          aGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAaggaggta                                         <  1:1839307/148‑3 (MQ=21)
                                                                                                           gAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGtaa                                        <  1:3147904/148‑1 (MQ=21)
                                                                                                           gAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGtaa                                        <  2:1011869/148‑1 (MQ=21)
                                                                                                             aTCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGtaaaa                                      >  2:1633034/1‑148 (MQ=21)
                                                                                                             aTCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGtaaaa                                      >  1:1452229/1‑148 (MQ=21)
                                                                                                              tCACTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAg                                     <  2:1507144/148‑1 (MQ=21)
                                                                                                                 cTCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATg                                  <  2:2365489/148‑1 (MQ=21)
                                                                                                                  tCCAAAAAGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGt                                 <  2:1400220/148‑1 (MQ=21)
                                                                                                                         aGCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGa                          >  1:1274520/1‑148 (MQ=21)
                                                                                                                          gCTATTGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGAc                         <  2:2978895/148‑1 (MQ=21)
                                                                                                                              ttGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGtt                     >  1:83355/1‑148 (MQ=21)
                                                                                                                              ttGATGAGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGACAAGACAAGGAGGTAAAAGATGTCAGTTGACCGtt                     >  2:2793380/1‑148 (MQ=18)
                                                                                                                               tgatgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTc                    >  1:2024851/1‑148 (MQ=21)
                                                                                                                               tgatgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTc                    >  1:4533235/1‑148 (MQ=18)
                                                                                                                               tgatgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTc                    <  1:3914959/148‑1 (MQ=21)
                                                                                                                               tgatgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTc                    <  2:2227653/148‑1 (MQ=21)
                                                                                                                                 atgaGATTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCat                  >  2:1433797/1‑148 (MQ=18)
                                                                                                                                   gagaTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCatac                >  1:618916/1‑147 (MQ=18)
                                                                                                                                     gaTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCatacag              >  2:1792143/1‑145 (MQ=18)
                                                                                                                                     gaTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAg              <  1:3456253/148‑1 (MQ=18)
                                                                                                                                      aTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCatacagc             >  1:3362388/1‑144 (MQ=18)
                                                                                                                                      aTTAGAGAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAgc             >  2:2728597/1‑147 (MQ=18)
                                                                                                                                         agagAAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAGGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGcccc          >  1:523371/1‑148 (MQ=18)
                                                                                                                                          gagaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt         <  2:1171676/148‑1 (MQ=18)
                                                                                                                                          gagaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt         <  1:1217660/148‑1 (MQ=18)
                                                                                                                                           agaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGTAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACCGCCCCTa        >  2:265718/1‑148 (MQ=17)
                                                                                                                                           agaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTa        <  2:3336187/148‑1 (MQ=18)
                                                                                                                                           agaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTa        >  2:798156/1‑148 (MQ=18)
                                                                                                                                                 |                                                                                                                                                   
GAGCGATTAGACAAATAGGGATAAGCGGGAATCAACTTTCTGATAGTTCTGTTAAACAACTGTCTTTATTTGAAAGTGTTCAAGAAAATCAAACTAATAAAAAGCAAGAATCACTCCAAAAAGCTATTGATGAGATTAGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGT  >  CP043523/1885517‑1885809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑