BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,798	T→C	S8S (AGT→AGC)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,798	0	T	C	99.7%	584.7 / NA	304	S8S (AGT→AGC)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (154/149); minor base G (0/1); total (154/150)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 305 total aligned reads displayed.

AGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885654‑1885931

 gagaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt                                                                                                                                    <  1:1217660/148‑1 (MQ=18)

 gagaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt                                                                                                                                    >  2:1302800/1‑148 (MQ=18)

 gagaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCt                                                                                                                                    <  1:2690701/148‑1 (MQ=18)

  agaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTa                                                                                                                                   >  2:798156/1‑148 (MQ=18)

  agaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTa                                                                                                                                   <  2:3336187/148‑1 (MQ=18)

   gaAACTTTCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTat                                                                                                                                  <  2:301823/148‑2 (MQ=18)

       ctttCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTatgaag                                                                                                                              <  2:2773404/146‑6 (MQ=17)

         ttCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTatgaagtc                                                                                                                            <  1:843561/148‑8 (MQ=17)

         ttCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTatgaagtc                                                                                                                            >  2:139748/1‑141 (MQ=17)

         ttCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtc                                                                                                                            >  2:2299899/1‑147 (MQ=17)

          tCGATTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGaagtca                                                                                                                           >  1:1499212/1‑143 (MQ=17)

             aTTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatcc                                                                                                                        >  2:2673847/1‑146 (MQ=17)

             aTTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatcc                                                                                                                        >  1:1798051/1‑146 (MQ=17)

              ttttCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatccg                                                                                                                       >  2:2844702/1‑145 (MQ=17)

              ttttCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatccg                                                                                                                       >  2:4071734/1‑145 (MQ=17)

               tttctttcAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACCAACTCATTGGAGGTCATGCAGCAAGTCAATACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatccgt                                                                                                                      >  1:344729/1‑144 (MQ=14)

                                        gtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGAtgatga                                                                                            >  2:2159793/1‑148 (MQ=14)

                                        gtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGAtgatga                                                                                            >  1:4094321/1‑148 (MQ=14)

                                         tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:2160498/148‑1 (MQ=9)

                                         tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:3059820/148‑1 (MQ=9)

                                         tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                           >  1:530145/1‑148 (MQ=14)

                                          ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                          >  1:2322025/1‑148 (MQ=14)

                                          ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                          <  1:1696591/148‑1 (MQ=9)

                                            aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATg                                                                                        >  1:4352374/1‑148 (MQ=14)

                                             tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       <  2:1516597/148‑1 (MQ=9)

                                               cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:449889/141‑1 (MQ=9)

                                               cGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:206999/148‑1 (MQ=14)

                                                gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATggggc                                                                                    >  1:3031443/7‑147 (MQ=9)

                                                  ggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGgcgc                                                                                  <  1:2265996/144‑1 (MQ=9)

                                                   gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1576746/145‑1 (MQ=9)

                                                   gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1063442/145‑1 (MQ=11)

                                                   gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1035787/4‑148 (MQ=14)

                                                    attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                                >  2:1154676/3‑148 (MQ=14)

                                                      tCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAttt                                                                              >  2:3716472/1‑148 (MQ=14)

                                                       cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:27673/148‑1 (MQ=14)

                                                       cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:4254719/148‑1 (MQ=14)

                                                        aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGc                                                                            <  2:932648/148‑1 (MQ=14)

                                                        aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGc                                                                            >  2:3479348/1‑148 (MQ=14)

                                                         cGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                           >  2:4221969/1‑148 (MQ=14)

                                                          gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          >  2:849014/1‑148 (MQ=14)

                                                           tgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGAc                                                                         >  2:4310196/1‑148 (MQ=14)

                                                            gtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACt                                                                        >  1:2313441/1‑148 (MQ=14)

                                                             tCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTg                                                                       >  2:201007/1‑148 (MQ=14)

                                                              cATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgg                                                                      >  1:964130/1‑148 (MQ=14)

                                                               aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  2:1714070/148‑2 (MQ=14)

                                                               aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  1:4044225/148‑2 (MQ=14)

                                                               aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  1:2937364/148‑2 (MQ=14)

                                                                tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    >  2:258747/1‑146 (MQ=14)

                                                                 ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                   <  2:3423982/148‑1 (MQ=9)

                                                                  tATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaa                                                                  <  2:2911611/148‑1 (MQ=9)

                                                                    tCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                                >  1:495335/1‑146 (MQ=14)

                                                                       gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcac                                                             <  2:3555454/148‑6 (MQ=9)

                                                                       gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcac                                                             <  2:1174783/148‑6 (MQ=9)

                                                                        aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  2:2559285/148‑7 (MQ=9)

                                                                        aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            >  2:3764657/1‑142 (MQ=9)

                                                                                    ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGAGCATACAGCCCCTAAGAAGTCATCCG‑TACTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAAt                                                <  1:1206208/148‑1 (MQ=9)

                                                                                     gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTGGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATa                                               <  1:2463684/148‑1 (MQ=2)

                                                                                      aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                              >  2:458571/1‑148 (MQ=2)

                                                                                       ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             <  1:4414843/148‑1 (MQ=255)

                                                                                       ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             >  1:1363456/1‑148 (MQ=2)

                                                                                        gTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                            >  1:4345606/1‑148 (MQ=255)

                                                                                         tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:4681264/148‑1 (MQ=2)

                                                                                         tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:3719186/148‑1 (MQ=2)

                                                                                          cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:1121448/1‑148 (MQ=9)

                                                                                          cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:3143404/1‑148 (MQ=9)

                                                                                           aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGGCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                         <  1:3350338/148‑1 (MQ=255)

                                                                                               agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                     <  1:1631205/148‑1 (MQ=255)

                                                                                                gcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaa                                    <  2:412250/148‑1 (MQ=9)

                                                                                                   aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 <  1:2644357/148‑1 (MQ=255)

                                                                                                    gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1417187/148‑1 (MQ=9)

                                                                                                     tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTTCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaa                               >  1:700408/1‑148 (MQ=9)

                                                                                                      cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaaa                              >  1:3617556/1‑148 (MQ=9)

                                                                                                         gACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                           >  2:4666855/1‑148 (MQ=9)

                                                                                                          aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          >  2:1881852/1‑148 (MQ=9)

                                                                                                          aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          <  2:1452229/148‑1 (MQ=9)

                                                                                                          aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          <  2:406519/148‑1 (MQ=9)

                                                                                                          aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGACAACAG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTAAAAAAAAAAAAAaag                          <  2:3960796/148‑1 (MQ=9)

                                                                                                           cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         >  2:3094676/1‑148 (MQ=9)

                                                                                                           cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         <  1:3220911/148‑1 (MQ=255)

                                                                                                            aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                        >  2:1025254/1‑147 (MQ=255)

                                                                                                            aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                        <  1:3917560/148‑2 (MQ=9)

                                                                                                              ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                      <  1:1884460/148‑1 (MQ=12)

                                                                                                               gaggtAAAATATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                     <  2:2080648/148‑1 (MQ=255)

                                                                                                               gaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                     <  1:3827059/148‑1 (MQ=255)

                                                                                                                aggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgata                    <  1:798109/148‑1 (MQ=12)

                                                                                                                 ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   <  1:2410604/145‑1 (MQ=9)

                                                                                                                 ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   >  2:2323879/4‑148 (MQ=9)

                                                                                                                   taaaaGATGTCAGTTGACCGTTAATACAGCCAATATGAAGTAATACG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:618916/147‑1 (MQ=255)

                                                                                                                   taaaaGATGGCAGTTGACCCTTCATACAGCCCCTATGAAGACATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTTGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 <  2:2794461/147‑1 (MQ=255)

                                                                                                                    aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                <  1:1981747/148‑2 (MQ=9)

                                                                                                                      aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaaa              >  1:721634/1‑145 (MQ=9)

                                                                                                                      aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATgataagaaaa              <  1:2859944/148‑1 (MQ=9)

                                                                                                                      aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTAAAAAAGAAAAAAAAGATgataagaaaa              <  1:1290253/148‑1 (MQ=9)

                                                                                                                        gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            >  1:959565/1‑148 (MQ=255)

                                                                                                                        gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            >  1:2614527/1‑148 (MQ=255)

                                                                                                                        gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            <  1:4135459/148‑1 (MQ=255)

                                                                                                                          tGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa          <  2:4409808/148‑1 (MQ=255)

                                                                                                                           gTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCaa         <  1:3494733/148‑1 (MQ=255)

                                                                                                                            tCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAg        <  2:1772092/148‑1 (MQ=255)

                                                                                                                                  gACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTAc  <  2:2389290/148‑1 (MQ=255)

AGAGAAACATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885654‑1885931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`