BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,807	G→A	E11E (GAG→GAA)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,807	0	G	A	99.6%	508.6 / NA	275	E11E (GAG→GAA)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (140/134); total (140/135)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

ATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885662‑1885931

aTTCGATTTTCTATCCATTCAAAAAGCAAGTAGTTTATCCGAAGGTTCACGTGTGATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGt                                                                                                                              <  2:3446857/148‑1 (MQ=11)

     aTTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatcc                                                                                                                         >  2:2673847/1‑146 (MQ=17)

      ttttCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatccg                                                                                                                        <  1:969621/148‑4 (MQ=17)

                             gtagtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGatga                                                                                               >  2:4354096/1‑148 (MQ=14)

                              tagtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGgatgat                                                                                              <  1:2148849/148‑1 (MQ=14)

                                gtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGAtgatga                                                                                            >  1:4094321/1‑148 (MQ=14)

                                 tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:3059820/148‑1 (MQ=9)

                                 tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                           >  1:530145/1‑148 (MQ=14)

                                  ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaaa                                                                                          >  1:392291/1‑148 (MQ=9)

                                  ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                          <  1:2904068/148‑1 (MQ=14)

                                   tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                         >  1:3866170/1‑148 (MQ=14)

                                   tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                         >  1:112008/1‑148 (MQ=9)

                                    aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATg                                                                                        >  1:4352374/1‑148 (MQ=14)

                                    aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATg                                                                                        >  2:3957817/1‑148 (MQ=14)

                                       cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:449889/141‑1 (MQ=9)

                                       cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     >  1:2336945/8‑148 (MQ=9)

                                       cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     >  1:3008907/8‑148 (MQ=9)

                                       cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATgggg                                                                                     >  1:1598553/8‑148 (MQ=14)

                                        gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATggggc                                                                                    >  2:1231262/7‑147 (MQ=9)

                                         aggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATggggcg                                                                                   <  1:2712640/143‑3 (MQ=9)

                                          ggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATggggcgc                                                                                  >  2:4227216/5‑145 (MQ=9)

                                           gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  2:243568/4‑148 (MQ=14)

                                           gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1062907/145‑1 (MQ=9)

                                           gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1035787/4‑148 (MQ=14)

                                            attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                                >  2:1154676/3‑148 (MQ=14)

                                             ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               >  1:4345688/2‑148 (MQ=14)

                                              tCACGTGTCATTTATCGGAACAAACTCATTGGAGGCCATGCAGCAAGCCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAttt                                                                              <  2:3754570/148‑1 (MQ=14)

                                               cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:27673/148‑1 (MQ=14)

                                               cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:752226/148‑1 (MQ=14)

                                               cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:4254719/148‑1 (MQ=14)

                                                 cGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                           >  1:2432447/1‑148 (MQ=14)

                                                  gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          >  2:1415097/1‑148 (MQ=14)

                                                  gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          <  2:4062288/148‑1 (MQ=14)

                                                     tCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTg                                                                       >  2:201007/1‑148 (MQ=14)

                                                       aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATTAAATGGGGCGCATTTGCGACttga                                                                     >  2:807399/1‑145 (MQ=14)

                                                        tttATCGGAACAAACTCATTGGAGGACATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    >  2:3702487/1‑146 (MQ=255)

                                                         ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggaga                                                                   >  1:3043774/1‑145 (MQ=14)

                                                          tATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaa                                                                  <  2:2911611/148‑1 (MQ=9)

                                                           aTCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaac                                                                 <  1:961159/148‑2 (MQ=9)

                                                            tCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                                >  1:495226/1‑146 (MQ=9)

                                                             cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               >  2:3189738/1‑145 (MQ=14)

                                                                aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  2:3553693/148‑7 (MQ=9)

                                                                 aCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                           <  2:412913/148‑8 (MQ=9)

                                                                             gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTGGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATa                                               <  1:2463684/148‑1 (MQ=2)

                                                                              aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                              >  2:458571/1‑148 (MQ=2)

                                                                              aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                              <  2:279826/148‑1 (MQ=2)

                                                                              aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                              <  1:3472363/148‑1 (MQ=2)

                                                                               ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             >  1:1364244/1‑148 (MQ=2)

                                                                               ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             <  1:4414843/148‑1 (MQ=255)

                                                                                 tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:3718484/148‑1 (MQ=2)

                                                                                 tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:1702171/148‑1 (MQ=2)

                                                                                 tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:4681264/148‑1 (MQ=2)

                                                                                 tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           >  2:2107114/1‑148 (MQ=2)

                                                                                  cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:3143404/1‑148 (MQ=9)

                                                                                  cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:3538473/1‑148 (MQ=2)

                                                                                   aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                         >  2:3025028/1‑148 (MQ=9)

                                                                                   aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGGCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                         <  1:3350338/148‑1 (MQ=255)

                                                                                     gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       >  2:4071376/1‑148 (MQ=9)

                                                                                     gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       <  2:961564/148‑1 (MQ=9)

                                                                                     gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       >  1:4069209/1‑148 (MQ=9)

                                                                                      cagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                      <  2:2256967/148‑1 (MQ=9)

                                                                                      cagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTGCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGTCGCATTTGCGACTGAAGAACTCACTGAGGCTCAAAATACTTGTgaaa                                      >  1:1518469/1‑148 (MQ=255)

                                                                                       agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                     <  1:216628/148‑1 (MQ=255)

                                                                                          aaGTCAAGACAAGGAGTTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                  <  2:1526055/148‑1 (MQ=9)

                                                                                          aaGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                  <  1:2321660/148‑1 (MQ=9)

                                                                                           aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 <  1:2644357/148‑1 (MQ=255)

                                                                                           aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 >  2:857055/1‑148 (MQ=9)

                                                                                            gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1417394/148‑1 (MQ=9)

                                                                                            gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                >  2:1740791/1‑148 (MQ=255)

                                                                                            gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1314753/148‑1 (MQ=9)

                                                                                             tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTTCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaa                               >  1:700408/1‑148 (MQ=9)

                                                                                              cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCAT‑GCT‑TATAACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaaaaaaa                              <  2:2577637/148‑1 (MQ=9)

                                                                                                 gACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                           >  2:4666855/1‑148 (MQ=9)

                                                                                                  aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          >  2:1881852/1‑148 (MQ=9)

                                                                                                   cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         >  2:3094676/1‑148 (MQ=9)

                                                                                                   cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         <  1:3220911/148‑1 (MQ=255)

                                                                                                   cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         <  1:343141/148‑1 (MQ=255)

                                                                                                    aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                        >  2:1025254/1‑147 (MQ=255)

                                                                                                     aggaggTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagatg                       >  1:1819822/1‑146 (MQ=9)

                                                                                                      ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                      <  1:1884460/148‑1 (MQ=12)

                                                                                                       gaggtAAAATATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                     <  2:2080648/148‑1 (MQ=255)

                                                                                                         ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   >  1:1155876/4‑148 (MQ=9)

                                                                                                         ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   >  2:2323879/4‑148 (MQ=9)

                                                                                                          gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  >  1:839984/3‑148 (MQ=9)

                                                                                                          gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  <  1:1231262/146‑1 (MQ=9)

                                                                                                          gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  >  1:2293771/3‑148 (MQ=9)

                                                                                                          gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  <  2:4073084/146‑1 (MQ=9)

                                                                                                           taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 >  2:1468276/2‑148 (MQ=9)

                                                                                                           taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:1372082/147‑1 (MQ=255)

                                                                                                           taaaaGATGTCAGTTGACCGTTAATACAGCCAATATGAAGTAAT‑ACG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:618916/147‑1 (MQ=255)

                                                                                                           taaaaGATGGCAGTTGACCCTTCATACAGCCCCTATGAAGACAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTTGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 <  2:2794461/147‑1 (MQ=255)

                                                                                                            aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                <  1:1981747/148‑2 (MQ=9)

                                                                                                             aaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaa               >  1:3755549/1‑146 (MQ=9)

                                                                                                              aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaaa              >  1:721634/1‑145 (MQ=9)

                                                                                                              aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATgataagaaaa              <  1:1646109/148‑1 (MQ=9)

                                                                                                                gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            >  1:2614527/1‑148 (MQ=255)

                                                                                                                gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            <  1:4135459/148‑1 (MQ=255)

                                                                                                                  tGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa          <  2:4409808/148‑1 (MQ=255)

                                                                                                                    tCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAg        <  2:1772092/148‑1 (MQ=255)

                                                                                                                          gACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTAc  <  2:2389290/148‑1 (MQ=255)

ATTCGATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885662‑1885931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`