BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,810	T→C	V12V (GTT→GTC)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,810	0	T	C	99.6%	497.8 / NA	266	V12V (GTT→GTC)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (133/132); minor base A (0/1); total (133/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

ATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885667‑1885931

aTTTTCTTTCAATACAAAAGGCAAGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGtcatcc                                                                                                                         >  1:1798051/1‑146 (MQ=17)

                        gtagtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGatga                                                                                               >  2:4354096/1‑148 (MQ=14)

                           gtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGAtgatga                                                                                            >  2:2317594/1‑148 (MQ=14)

                            tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:2160498/148‑1 (MQ=9)

                            tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:3059820/148‑1 (MQ=9)

                            tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                           <  2:2239899/148‑1 (MQ=14)

                              tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                         >  1:112008/1‑148 (MQ=9)

                               aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATg                                                                                        <  1:2633136/148‑1 (MQ=9)

                               aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATg                                                                                        <  1:3465910/148‑1 (MQ=9)

                               aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATg                                                                                        >  2:3957817/1‑148 (MQ=14)

                                tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       <  1:1212382/148‑1 (MQ=9)

                                  cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:449820/141‑1 (MQ=9)

                                  cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATgggg                                                                                     >  1:1598553/8‑148 (MQ=14)

                                  cGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:206999/148‑1 (MQ=14)

                                   gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATggggc                                                                                    >  1:3031443/7‑147 (MQ=9)

                                   gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATggggc                                                                                    >  1:489229/7‑147 (MQ=14)

                                     ggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATggggcgc                                                                                  >  2:4227216/5‑145 (MQ=9)

                                      gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1062907/145‑1 (MQ=9)

                                      gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  2:243568/4‑148 (MQ=14)

                                      gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1504845/4‑148 (MQ=14)

                                      gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1035787/4‑148 (MQ=14)

                                       attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                                <  1:4204362/146‑1 (MQ=9)

                                       attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                                <  2:137409/146‑1 (MQ=9)

                                        ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               >  1:4346069/2‑148 (MQ=14)

                                        ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               <  1:4113844/147‑1 (MQ=14)

                                          cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:27673/148‑1 (MQ=14)

                                          cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  2:2024851/148‑1 (MQ=14)

                                           aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCTCGATAGAGGGATGATGAAATGGGGCGCGTTTGc                                                                            >  2:2086698/1‑148 (MQ=14)

                                            cGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                           >  2:2633168/1‑148 (MQ=14)

                                             gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          <  2:4062288/148‑1 (MQ=14)

                                              tgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGAc                                                                         >  2:2986703/1‑148 (MQ=14)

                                                 cATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgg                                                                      >  2:1591650/1‑148 (MQ=14)

                                                  aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATTAAATGGGGCGCATTTGCGACttga                                                                     >  2:807399/1‑145 (MQ=14)

                                                  aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  1:2937364/148‑2 (MQ=14)

                                                   tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    <  1:2322851/148‑3 (MQ=9)

                                                   tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    >  1:3310571/1‑146 (MQ=14)

                                                   tttATCGGAACAAACTCATTGGAGGACATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    >  2:3702487/1‑146 (MQ=255)

                                                    ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                   <  2:3423982/148‑1 (MQ=9)

                                                       tCGGAACAACCTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                                <  1:3132988/148‑3 (MQ=9)

                                                        cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               <  2:1007668/148‑4 (MQ=9)

                                                        cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               >  1:4541925/1‑145 (MQ=14)

                                                        cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               >  2:3189738/1‑145 (MQ=14)

                                                         ggAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactca                                                              >  2:2870570/1‑144 (MQ=14)

                                                          gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcac                                                             <  2:1174783/148‑6 (MQ=9)

                                                           aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  1:35277/148‑7 (MQ=9)

                                                           aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  2:3553693/148‑7 (MQ=9)

                                                           aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            >  2:3764657/1‑142 (MQ=9)

                                                            aCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                           <  2:412913/148‑8 (MQ=9)

                                                            aCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                           >  1:961564/1‑141 (MQ=2)

                                                                       ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAAt                                                >  1:523330/1‑148 (MQ=2)

                                                                        gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTGGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATa                                               <  1:2463684/148‑1 (MQ=2)

                                                                         aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                              <  2:279826/148‑1 (MQ=2)

                                                                         aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                              >  2:458571/1‑148 (MQ=2)

                                                                          ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             >  1:1364244/1‑148 (MQ=2)

                                                                          ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             <  1:4088061/148‑1 (MQ=255)

                                                                           gTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtt                                            >  1:4345606/1‑148 (MQ=255)

                                                                            tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:1702171/148‑1 (MQ=2)

                                                                            tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  2:1750301/148‑1 (MQ=2)

                                                                              aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                         >  2:2780743/1‑148 (MQ=9)

                                                                               tGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTga                                        <  2:10871/148‑1 (MQ=9)

                                                                                gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       >  2:4071376/1‑148 (MQ=9)

                                                                                gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       <  1:3673557/148‑1 (MQ=9)

                                                                                gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       <  2:961564/148‑1 (MQ=9)

                                                                                    caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaag                                   >  1:4046602/1‑148 (MQ=9)

                                                                                    caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACGTTTgaaaaag                                   >  1:706629/1‑148 (MQ=9)

                                                                                     aaGTCAAGACAAGGGCGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTGTGAGGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                  <  2:3086416/148‑1 (MQ=9)

                                                                                     aaGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                  <  1:2321660/148‑1 (MQ=9)

                                                                                      aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 <  2:1845188/148‑1 (MQ=255)

                                                                                      aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 >  2:857055/1‑148 (MQ=9)

                                                                                      aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 >  1:65531/1‑148 (MQ=9)

                                                                                       gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1417394/148‑1 (MQ=9)

                                                                                       gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1314753/148‑1 (MQ=9)

                                                                                       gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTAgaaaaagaaa                                <  2:2386031/148‑1 (MQ=9)

                                                                                         cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCAT‑GCT‑TATAACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaaaaaaa                              <  2:2577637/148‑1 (MQ=9)

                                                                                           aGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAaaaaaaaaaaaa                            <  2:1983698/148‑1 (MQ=9)

                                                                                            gACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                           >  2:4666855/1‑148 (MQ=9)

                                                                                            gACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                           >  2:1281045/1‑148 (MQ=9)

                                                                                             aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          >  1:4394197/1‑148 (MQ=9)

                                                                                             aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          <  2:1452229/148‑1 (MQ=9)

                                                                                              cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         <  1:343141/148‑1 (MQ=255)

                                                                                               aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                        <  1:3917560/148‑2 (MQ=9)

                                                                                                 ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                      <  1:1884460/148‑1 (MQ=12)

                                                                                                  gaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                     <  1:3827059/148‑1 (MQ=255)

                                                                                                   aggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgata                    <  1:798156/148‑1 (MQ=9)

                                                                                                    ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   <  1:2410604/145‑1 (MQ=9)

                                                                                                    ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   >  1:1155876/4‑148 (MQ=9)

                                                                                                     gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  >  1:2293771/3‑148 (MQ=9)

                                                                                                     gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  <  2:4073084/146‑1 (MQ=9)

                                                                                                      taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 <  1:3199680/147‑1 (MQ=255)

                                                                                                      taaaaGATGTCAGTTGACCGTTAATACAGCCAATATGAAGTAAT‑ACG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:618916/147‑1 (MQ=255)

                                                                                                       aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                <  1:1981747/148‑2 (MQ=9)

                                                                                                        aaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaa               >  1:3755549/1‑146 (MQ=9)

                                                                                                         aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATgataagaaaa              <  1:1646109/148‑1 (MQ=9)

                                                                                                         aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATgataagaaaa              <  1:2859944/148‑1 (MQ=9)

                                                                                                           gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            >  1:2614527/1‑148 (MQ=255)

                                                                                                           gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            >  1:959565/1‑148 (MQ=255)

                                                                                                            aTGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTc           >  1:2665073/1‑148 (MQ=255)

                                                                                                             tGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa          >  1:751431/1‑148 (MQ=255)

                                                                                                             tGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa          <  2:4409808/148‑1 (MQ=255)

                                                                                                                 aGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGct      <  2:1499212/148‑2 (MQ=255)

ATTTTCTATCTATACAAAAAGCAAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885667‑1885931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`