BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,834	A→G	G20G (GGA→GGG)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,834	0	A	G	99.4%	661.2 / NA	312	G20G (GGA→GGG)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (155/153); total (156/156)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

AAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCA  >  CP043523/1885689‑1885977

aaGTAGTTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGat                                                                                                                                               >  2:3670296/1‑148 (MQ=14)

     gtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGAtgatga                                                                                                                                          >  1:4094771/1‑148 (MQ=9)

      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                                                                         <  1:2160498/148‑1 (MQ=9)

      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                                                                         <  1:3016658/148‑1 (MQ=9)

      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                                                                         <  2:2239899/148‑1 (MQ=14)

       ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGaaa                                                                                                                                        >  1:392291/1‑148 (MQ=9)

       ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                                                                        <  1:2904068/148‑1 (MQ=14)

       ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                                                                        >  1:2057677/1‑148 (MQ=14)

       ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                                                                        >  1:859667/1‑148 (MQ=14)

        tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                                                                       >  1:3866170/1‑148 (MQ=14)

            cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                                                                   <  1:449820/141‑1 (MQ=9)

             gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATggggc                                                                                                                                  >  1:489229/7‑147 (MQ=14)

             gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATggggc                                                                                                                                  >  1:2313519/7‑147 (MQ=14)

              aggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATggggcg                                                                                                                                 <  1:2712640/143‑3 (MQ=9)

               ggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGgcgc                                                                                                                                <  1:2265996/144‑1 (MQ=9)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                                                               >  1:1504845/4‑148 (MQ=14)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                                                               <  1:1063442/145‑1 (MQ=11)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                                                               >  2:2743521/4‑148 (MQ=14)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                                                               >  1:1035787/4‑148 (MQ=14)

                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                                                                             >  1:4346069/2‑148 (MQ=14)

                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                                                                             >  1:3158717/2‑148 (MQ=14)

                    cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                                                                           <  2:2024851/148‑1 (MQ=14)

                     aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGc                                                                                                                          <  2:932648/148‑1 (MQ=14)

                      cGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                                                                         >  2:4221398/1‑148 (MQ=14)

                        tgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGAc                                                                                                                       >  2:2986703/1‑148 (MQ=14)

                          tCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTg                                                                                                                     >  2:201007/1‑148 (MQ=14)

                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATTAAATGGGGCGCATTTGCGACttga                                                                                                                   >  2:807399/1‑145 (MQ=14)

                             tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                                                                  <  1:2322851/148‑3 (MQ=9)

                             tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                                                                  >  2:258747/1‑146 (MQ=14)

                              ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                                                                 <  2:3423982/148‑1 (MQ=9)

                              ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGgaga                                                                                                                 <  1:1928229/148‑1 (MQ=9)

                              ttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggaga                                                                                                                 >  1:3043774/1‑145 (MQ=14)

                               tATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaa                                                                                                                <  2:608426/148‑1 (MQ=9)

                                 tCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                                                                              <  1:1608350/148‑3 (MQ=14)

                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                                                                             <  2:4275548/148‑4 (MQ=9)

                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                                                                             >  1:4541925/1‑145 (MQ=14)

                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                                                                             >  2:3189738/1‑145 (MQ=14)

                                   ggAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactca                                                                                                            >  2:2870570/1‑144 (MQ=14)

                                    gAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcac                                                                                                           <  2:1174783/148‑6 (MQ=9)

                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                                                                          <  2:3553693/148‑7 (MQ=9)

                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                                                                          <  2:2559285/148‑7 (MQ=9)

                                      aCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacgg                                                                                                         >  1:961564/1‑141 (MQ=2)

                                                 ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAAt                                                                                              >  1:523330/1‑148 (MQ=2)

                                                 ggAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGAGCATACAGCCCCTAAGAAGTCATCCG‑TACTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAAt                                                                                              <  1:1206208/148‑1 (MQ=9)

                                                  gAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTGGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATa                                                                                             <  1:2463684/148‑1 (MQ=2)

                                                   aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                                                                            >  2:458571/1‑148 (MQ=2)

                                                   aGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATAc                                                                                            <  1:3472363/148‑1 (MQ=2)

                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                                                                           >  1:1363456/1‑148 (MQ=2)

                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                                                                         <  1:1702171/148‑1 (MQ=2)

                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                                                                        >  1:1121448/1‑148 (MQ=9)

                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                                                                        >  1:3131464/1‑148 (MQ=9)

                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                                                                        >  1:3538473/1‑148 (MQ=2)

                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                                                                     >  1:3268670/1‑148 (MQ=9)

                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                                                                     >  1:4069209/1‑148 (MQ=9)

                                                            agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                                                                   <  1:3396667/148‑1 (MQ=255)

                                                             gcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaa                                                                                  <  2:412250/148‑1 (MQ=9)

                                                              caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACGTTTgaaaaag                                                                                 >  1:706629/1‑148 (MQ=9)

                                                               aaGTCAAGACAAGGAGTTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                                                                <  2:1526055/148‑1 (MQ=9)

                                                                aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                                                               <  2:1845188/148‑1 (MQ=255)

                                                                aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                                                               >  2:857055/1‑148 (MQ=9)

                                                                aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                                                               >  1:65531/1‑148 (MQ=9)

                                                                 gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                                                              <  1:1417187/148‑1 (MQ=9)

                                                                 gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                                                              >  2:1740791/1‑148 (MQ=255)

                                                                      gACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                                                                         >  2:4666855/1‑148 (MQ=9)

                                                                       aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                                                                        >  1:4394197/1‑148 (MQ=9)

                                                                       aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                                                                        <  2:406519/148‑1 (MQ=9)

                                                                       aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGACAACAG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTAAAAAAAAAAAAAaag                                                                        <  2:3960796/148‑1 (MQ=9)

                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGGCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGTATGTTGAAATGGGGCGCAGTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                                                                       <  1:4157984/148‑1 (MQ=255)

                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                                                                       >  2:2795356/1‑148 (MQ=9)

                                                                         aaGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaagat                                                                      >  2:1025254/1‑147 (MQ=255)

                                                                            gaggtAAAATATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                                                                   <  2:2080648/148‑1 (MQ=255)

                                                                            gaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                                                                   >  2:1449600/1‑148 (MQ=9)

                                                                             aggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgata                                                                  <  1:798156/148‑1 (MQ=9)

                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                                                                 <  1:2410604/145‑1 (MQ=9)

                                                                               gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                                                                <  2:4073084/146‑1 (MQ=9)

                                                                                taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                                                               <  2:1372082/147‑1 (MQ=255)

                                                                                 aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                                                              <  1:2648924/148‑2 (MQ=9)

                                                                                  aaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaa                                                             >  1:3755549/1‑146 (MQ=9)

                                                                                   aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATgataagaaaa                                                            <  1:1646109/148‑1 (MQ=9)

                                                                                   aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTAAAAAAGAAAAAAAAGATgataagaaaa                                                            <  1:1290253/148‑1 (MQ=9)

                                                                                     gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt                                                          >  1:2614527/1‑148 (MQ=255)

                                                                                       tGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa                                                        >  1:751431/1‑148 (MQ=255)

                                                                                       tGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCa                                                        <  2:4409808/148‑1 (MQ=255)

                                                                                              tGACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTa                                                 <  2:3869522/148‑1 (MQ=255)

                                                                                               gACCGTTCATACAGCCCCTATGAAGTCATCCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTAc                                                <  2:2389290/148‑1 (MQ=255)

                                                                                                                                tgcttATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAg                >  2:1968328/5‑148 (MQ=255)

                                                                                                                                 gcttATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGt               <  2:2090673/145‑1 (MQ=255)

                                                                                                                                  cttATCACGAAAGAGGGATGATGAAAGGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTc              <  1:3636333/146‑1 (MQ=255)

                                                                                                                                   ttATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGATCTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAATAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTct             <  2:1345125/147‑2 (MQ=255)

                                                                                                                                     aTCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCttt           >  2:3723482/1‑148 (MQ=255)

                                                                                                                                     aTCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCttt           <  1:2815751/148‑1 (MQ=255)

                                                                                                                                       cACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTtttt         <  1:4221969/148‑1 (MQ=255)

                                                                                                                                        aCGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTc        <  1:4345398/148‑1 (MQ=255)

                                                                                                                                         cGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTca       <  2:112008/148‑2 (MQ=255)

                                                                                                                                          gATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTACTa      >  1:2984364/1‑148 (MQ=255)

                                                                                                                                            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt    >  1:1802738/1‑148 (MQ=255)

                                                                                                                                            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt    >  2:3034124/1‑148 (MQ=255)

                                                                                                                                            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt    <  1:1880564/148‑1 (MQ=255)

                                                                                                                                            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt    >  2:1477821/1‑148 (MQ=255)

                                                                                                                                            tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt    <  2:2336945/148‑1 (MQ=255)

AAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCA  >  CP043523/1885689‑1885977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`