BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,972	T→A	S66S (TCT→TCA)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,972	0	T	A	98.5%	879.5 / ‑0.3	338	S66S (TCT→TCA)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (4/1); new base A (164/168); total (168/170)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

GATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAAT  >  CP043523/1885826‑1886116

 aTAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCaaa                                                                                                                                                >  1:1794593/1‑148 (MQ=255)

  tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt                                                                                                                                               >  2:3034124/1‑148 (MQ=255)

   agagGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATc                                                                                                                                              >  2:2017968/1‑148 (MQ=255)

   agagGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATc                                                                                                                                              >  2:532207/1‑148 (MQ=255)

    gagGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCa                                                                                                                                             <  1:3031594/148‑1 (MQ=255)

        gatgatgAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgcta                                                                                                                                         >  2:670624/2‑145 (MQ=255)

        gatgatgAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgcta                                                                                                                                         >  1:3663862/2‑145 (MQ=255)

         atgatgAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctac                                                                                                                                        >  1:4301914/1‑144 (MQ=255)

          tgatgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctaca                                                                                                                                       >  1:3203267/1‑143 (MQ=255)

            atgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctacaaa                                                                                                                                     <  2:71406/148‑8 (MQ=255)

            atgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACaaa                                                                                                                                     <  2:4533235/148‑1 (MQ=255)

             tgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAt                                                                                                                                    <  1:201007/148‑1 (MQ=255)

              gaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtc                                                                                                                                   >  2:4215874/1‑147 (MQ=255)

                aaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtcaa                                                                                                                                 >  2:3855520/1‑145 (MQ=255)

                aaTGGGCCGCATTGGGAACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaa                                                                                                                                 <  1:243568/148‑1 (MQ=255)

                 aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                >  1:1473299/1‑148 (MQ=255)

                 aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                >  1:1360633/1‑148 (MQ=255)

                 aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                >  1:3113949/1‑148 (MQ=255)

                    gggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGtt                                                                                                                             >  2:858818/5‑148 (MQ=255)

                     ggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTATCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagtta                                                                                                                            <  1:1415097/145‑5 (MQ=255)

                      gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                           >  2:1395826/3‑143 (MQ=255)

                      gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                           <  1:1433797/146‑6 (MQ=255)

                       cgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGttaaa                                                                                                                          >  1:1537414/2‑145 (MQ=255)

                        gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                         <  1:2780743/148‑8 (MQ=255)

                        gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                         <  2:3941984/148‑8 (MQ=255)

                         cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                                                        >  1:3983045/1‑148 (MQ=255)

                            ttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCa                                                                                                                     >  1:2913535/1‑148 (MQ=255)

                            ttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCa                                                                                                                     >  2:555132/1‑148 (MQ=255)

                             tGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaa                                                                                                                    <  2:2920411/148‑1 (MQ=255)

                                 aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatcaa                                                                                                                <  1:2317594/148‑5 (MQ=255)

                                 aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCGCAATCAGTCTTTTTCAAATCAGCTACAAATCAAGGTTAAATATCaatcaa                                                                                                                >  2:4437787/1‑144 (MQ=255)

                                                gaggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATg                                                                                                 <  2:950458/142‑1 (MQ=255)

                                                  ggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGaa                                                                                               >  1:1699494/5‑148 (MQ=255)

                                                   gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                              <  2:4069490/145‑1 (MQ=255)

                                                   gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                              >  2:885035/4‑148 (MQ=255)

                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            >  2:3416312/2‑148 (MQ=255)

                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            >  2:4525032/2‑148 (MQ=255)

                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            <  1:3542210/147‑1 (MQ=255)

                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            >  1:2589944/2‑148 (MQ=255)

                                                       aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                          <  2:495335/148‑1 (MQ=255)

                                                       aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                          <  2:2313519/148‑1 (MQ=255)

                                                        aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                         >  1:2075871/1‑148 (MQ=255)

                                                         aaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATg                                                                                        >  2:1190749/1‑148 (MQ=255)

                                                          aTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATgg                                                                                       >  1:2280189/1‑148 (MQ=255)

                                                           tACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGt                                                                                      >  1:1871600/1‑148 (MQ=255)

                                                             cTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttt                                                                                    >  1:4145462/1‑146 (MQ=255)

                                                             cTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttt                                                                                    <  1:458571/148‑3 (MQ=255)

                                                               tttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttg                                                                                  >  2:1484547/1‑144 (MQ=255)

                                                                ttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttgt                                                                                 >  2:1032865/1‑143 (MQ=255)

                                                                                 gatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaata                                                                >  2:1970911/4‑148 (MQ=9)

                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              >  1:4556284/2‑148 (MQ=9)

                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              <  1:3469513/147‑1 (MQ=9)

                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              >  2:1787726/2‑148 (MQ=9)

                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              >  2:960757/2‑148 (MQ=9)

                                                                                      taagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCaa                                                           <  2:822249/148‑1 (MQ=9)

                                                                                         gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                                                        <  1:4069269/145‑1 (MQ=9)

                                                                                         gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                                                        <  2:1802738/145‑1 (MQ=9)

                                                                                          aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                       <  2:4285257/146‑1 (MQ=9)

                                                                                          aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                       >  2:48024/3‑148 (MQ=9)

                                                                                          aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                       <  1:1281045/146‑1 (MQ=9)

                                                                                           aaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAag                                                      <  2:1121449/147‑1 (MQ=9)

                                                                                             aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                    >  1:180807/1‑148 (MQ=9)

                                                                                               tCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGtt                                                  <  1:4692355/148‑1 (MQ=9)

                                                                                                 aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                >  2:4362629/1‑148 (MQ=9)

                                                                                                  aGCATTACCACACCATGTAGTCCCTTGTCTTCTCCATCAGTCTTTTTCAAATCAGCTCCAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaaa                                               >  2:3250012/1‑148 (MQ=9)

                                                                                                   gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt                                              <  2:3594891/145‑1 (MQ=9)

                                                                                                   gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt                                              <  2:3462887/145‑1 (MQ=9)

                                                                                                     tttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCa                                            <  1:2737532/147‑1 (MQ=9)

                                                                                                        aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg                                         >  2:145549/1‑148 (MQ=9)

                                                                                                          cacaTCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGa                                       >  2:2874567/1‑148 (MQ=9)

                                                                                                              tcatgTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACACATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGTTaaa                                   <  1:1090854/148‑1 (MQ=9)

                                                                                                                   tagtccTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatcta                              <  2:65531/143‑1 (MQ=12)

                                                                                                                     gtcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                            >  1:3777867/8‑148 (MQ=12)

                                                                                                                     gtcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                            >  1:746774/8‑148 (MQ=9)

                                                                                                                     gtccTTTATCTTCTCAATCAGTCTTTTTCCAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                            <  2:4161714/145‑1 (MQ=12)

                                                                                                                      tcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGACGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatct                           >  1:941289/7‑148 (MQ=17)

                                                                                                                      tcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatct                           >  2:1657044/7‑148 (MQ=12)

                                                                                                                       cctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTAtctc                          >  1:102600/6‑148 (MQ=14)

                                                                                                                        ctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCa                         >  2:2852058/5‑148 (MQ=12)

                                                                                                                        cTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCa                         <  2:3768568/148‑1 (MQ=12)

                                                                                                                         tttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCat                        <  1:2111934/145‑1 (MQ=17)

                                                                                                                           taTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCatat                      >  1:174873/2‑148 (MQ=12)

                                                                                                                            aTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATc                     <  1:1315989/148‑1 (MQ=17)

                                                                                                                             tcttctCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATcc                    >  2:2439588/1‑148 (MQ=17)

                                                                                                                                tctcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAtt                 >  2:2704041/8‑148 (MQ=18)

                                                                                                                                  tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga               >  2:4678934/6‑146 (MQ=18)

                                                                                                                                  tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga               >  2:2596956/6‑146 (MQ=18)

                                                                                                                                   caatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaa              <  1:670624/144‑4 (MQ=18)

                                                                                                                                    aatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaac             <  2:2189305/145‑5 (MQ=18)

                                                                                                                                      tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa           <  2:3521361/147‑7 (MQ=18)

                                                                                                                                      tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa           <  1:2803405/147‑7 (MQ=18)

                                                                                                                                          tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta       <  2:2573369/145‑3 (MQ=18)

                                                                                                                                          tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta       >  2:3157687/4‑146 (MQ=18)

                                                                                                                                           ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCATAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat      <  2:2389851/146‑4 (MQ=255)

                                                                                                                                           ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat      <  2:1343381/146‑4 (MQ=16)

                                                                                                                                           ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat      <  1:3327189/146‑4 (MQ=16)

                                                                                                                                             ttttCAAATCATCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataa    <  2:1301654/148‑6 (MQ=18)

                                                                                                                                             ttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataa    <  2:3326064/148‑6 (MQ=18)

                                                                                                                                              tttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataaa   <  2:3663862/148‑7 (MQ=18)

                                                                                                                                              tttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataaa   <  1:4387449/148‑7 (MQ=16)

GATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAAT  >  CP043523/1885826‑1886116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`