BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,981	G→A	V69L (GTG→CTA)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,981	0	G	A	99.7%	796.0 / NA	315	V69L (GTG→CTA)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (150/164); minor base C (1/0); total (151/164)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

ATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATATATC  >  CP043523/1885838‑1886121

 tgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAt                                                                                                                                         <  1:258747/148‑1 (MQ=255)

 tgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAt                                                                                                                                         <  1:201007/148‑1 (MQ=255)

  gaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtc                                                                                                                                        >  2:4215874/1‑147 (MQ=255)

    aaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtcaa                                                                                                                                      >  2:3855520/1‑145 (MQ=255)

    aaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaa                                                                                                                                      <  1:4604175/148‑1 (MQ=255)

     aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                     >  1:1473299/1‑148 (MQ=255)

       ggggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagt                                                                                                                                   <  1:1154676/148‑3 (MQ=255)

        gggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGtt                                                                                                                                  >  2:858818/5‑148 (MQ=255)

         ggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagtta                                                                                                                                 >  1:3138421/4‑144 (MQ=255)

          gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                                <  2:2057677/146‑6 (MQ=255)

          gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                                <  1:1433797/146‑6 (MQ=255)

           cgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGttaaa                                                                                                                               >  1:1537247/2‑145 (MQ=255)

            gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                              <  1:1792066/148‑8 (MQ=255)

            gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                              <  1:2780743/148‑8 (MQ=255)

             cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                                                             <  2:4346069/148‑1 (MQ=255)

             cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                                                             <  2:4394197/148‑1 (MQ=255)

              aTTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAatat                                                                                                                            <  1:807399/148‑1 (MQ=255)

              aTTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAatat                                                                                                                            >  2:219536/1‑148 (MQ=255)

               tttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATc                                                                                                                           >  1:2294473/1‑148 (MQ=255)

               tttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTACTAATCAAGTACAAGTCAAAATTAAATATc                                                                                                                           >  1:4425459/1‑148 (MQ=9)

                 tGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaa                                                                                                                         >  2:1507841/1‑148 (MQ=255)

                 tGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaa                                                                                                                         <  1:411461/148‑1 (MQ=255)

                     aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatcaa                                                                                                                     <  2:1788091/148‑5 (MQ=255)

                     aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatcaa                                                                                                                     <  1:2317594/148‑5 (MQ=255)

                                  cggaggCCCAAAATACTTTTGAAAAAAAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTaaa                                                                                                        <  2:344729/143‑2 (MQ=255)

                                    gaggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATg                                                                                                      <  2:950458/142‑1 (MQ=255)

                                     aggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGa                                                                                                     >  1:172259/6‑148 (MQ=255)

                                       gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                                   >  1:4230960/4‑148 (MQ=255)

                                       gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                                   >  2:885035/4‑148 (MQ=255)

                                         tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                                 >  1:3627745/2‑148 (MQ=255)

                                         tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                                 <  1:3764657/147‑1 (MQ=255)

                                           aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                               <  2:2313519/148‑1 (MQ=255)

                                           aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                               >  1:1663341/1‑148 (MQ=255)

                                            aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                              <  2:489229/148‑1 (MQ=255)

                                            aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                              <  2:2864727/148‑1 (MQ=255)

                                            aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                              >  1:2075871/1‑148 (MQ=255)

                                             aaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATg                                                                                             >  2:140472/1‑148 (MQ=255)

                                             aaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATg                                                                                             >  2:1190749/1‑148 (MQ=255)

                                              aTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATgg                                                                                            <  2:3043774/148‑1 (MQ=255)

                                               tACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGt                                                                                           >  1:1871600/1‑148 (MQ=255)

                                                aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGCAGTCAATggcc                                                                                          >  1:4003264/1‑146 (MQ=255)

                                                aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtt                                                                                          <  1:4576993/148‑2 (MQ=255)

                                                aCTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtt                                                                                          >  1:4380614/1‑147 (MQ=255)

                                                 cTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttt                                                                                         >  2:4565679/1‑146 (MQ=255)

                                                  ttttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGtttt                                                                                        >  1:1309051/1‑145 (MQ=255)

                                                    ttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttgt                                                                                      >  2:1032865/1‑143 (MQ=255)

                                                                    agatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaat                                                                      <  2:4069209/144‑1 (MQ=9)

                                                                     gatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaata                                                                     >  2:1970911/4‑148 (MQ=9)

                                                                      atgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataa                                                                    >  1:659573/3‑148 (MQ=9)

                                                                       tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                                   >  2:960757/2‑148 (MQ=9)

                                                                        gataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATc                                                                  >  2:549314/1‑148 (MQ=9)

                                                                         ataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCa                                                                 <  2:1659376/148‑1 (MQ=9)

                                                                          taagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCaa                                                                >  2:3217202/1‑148 (MQ=9)

                                                                          taagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCaa                                                                <  2:822249/148‑1 (MQ=9)

                                                                           aagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAg                                                               >  1:3406280/1‑148 (MQ=9)

                                                                            agaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGt                                                              >  2:4472658/5‑148 (MQ=2)

                                                                             gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                                                             >  2:3729363/4‑148 (MQ=2)

                                                                              aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                            <  2:4285257/146‑1 (MQ=9)

                                                                               aaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAag                                                           <  2:1121448/147‑1 (MQ=9)

                                                                                 aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                         >  2:365547/1‑148 (MQ=9)

                                                                                 aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                         >  1:180807/1‑148 (MQ=9)

                                                                                 aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                         <  1:1395826/148‑1 (MQ=9)

                                                                                   tCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGtt                                                       >  2:1302160/1‑148 (MQ=9)

                                                                                    cAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTa                                                      >  1:3041225/1‑148 (MQ=9)

                                                                                     aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCGGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                     <  2:1819822/148‑1 (MQ=9)

                                                                                     aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                     >  2:528906/1‑148 (MQ=9)

                                                                                     aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTAAAAACAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTAGCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                     <  1:3947006/148‑1 (MQ=9)

                                                                                       gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt                                                   <  2:3594891/145‑1 (MQ=9)

                                                                                         tttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCa                                                 <  1:2737532/147‑1 (MQ=9)

                                                                                            aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg                                              >  2:145549/1‑148 (MQ=9)

                                                                                            aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg                                              <  2:2665073/148‑1 (MQ=9)

                                                                                               acaTCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGAt                                           >  2:392387/1‑148 (MQ=9)

                                                                                                      gtagtccTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatct                                    <  2:706629/142‑1 (MQ=14)

                                                                                                       tagtccTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatcta                                   <  2:65531/143‑1 (MQ=12)

                                                                                                         gtcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                                 <  2:4700533/141‑1 (MQ=12)

                                                                                                         gtccTTTATCTTCTCAATCAGTCTTTTTCCAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                                 <  2:4161714/145‑1 (MQ=12)

                                                                                                          tcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGACGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatct                                >  1:941289/7‑148 (MQ=17)

                                                                                                           cctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTAtctc                               <  2:2913883/143‑1 (MQ=12)

                                                                                                           cctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTAtctc                               >  1:102600/6‑148 (MQ=14)

                                                                                                            ctttaTCTTCTCAATCAGTCTTTTTCTTATTTTCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACTGATAAAATCTATCTCa                              >  2:328760/5‑148 (MQ=9)

                                                                                                             tttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCat                             <  1:2111934/145‑1 (MQ=17)

                                                                                                                aTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATc                          <  1:1315989/148‑1 (MQ=17)

                                                                                                                 tcttctCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATcc                         >  2:2439588/1‑148 (MQ=17)

                                                                                                                      tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAGATGAAGTCTATGGTTTTGTATCAGAATTTATCAATTATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga                    >  2:4529035/6‑146 (MQ=17)

                                                                                                                      tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga                    >  2:2596956/6‑146 (MQ=18)

                                                                                                                       caatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaa                   <  1:672976/144‑4 (MQ=17)

                                                                                                                         atcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaaca                 >  1:2880260/3‑143 (MQ=18)

                                                                                                                          tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa                <  2:3521057/147‑7 (MQ=18)

                                                                                                                          tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa                <  1:2803376/147‑7 (MQ=18)

                                                                                                                            aGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAt              >  2:2849542/1‑148 (MQ=17)

                                                                                                                              tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta            <  2:2573369/145‑3 (MQ=18)

                                                                                                                              tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta            <  1:3517190/145‑3 (MQ=16)

                                                                                                                               ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCATAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat           <  2:2389851/146‑4 (MQ=255)

                                                                                                                               ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat           <  2:1343381/146‑4 (MQ=16)

                                                                                                                               ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat           <  1:3326256/146‑4 (MQ=16)

                                                                                                                                 ttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataa         <  2:3326301/148‑6 (MQ=16)

                                                                                                                                   ttcaaaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAat       >  2:556627/5‑148 (MQ=12)

                                                                                                                                    tcaaaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAata      <  1:1084807/145‑1 (MQ=17)

                                                                                                                                      aaaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAatatt    <  2:3138421/147‑2 (MQ=12)

                                                                                                                                       aaTCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAatattt   <  1:1354417/148‑3 (MQ=12)

ATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATATATC  >  CP043523/1885838‑1886121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`