BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,891,084	A→G	Y49Y (TAT→TAC)	FTN78_chr1940 ←	Mobile element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,891,084	0	A	G	99.5%	423.7 / NA	200	Y49Y (TAT→TAC)	FTN78_chr1940	Mobile element protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (98/101); minor base T (0/1); total (98/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

GTAAGAGGGCATTACAACGGCGGACAAAGCGAGAATATTCTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGATAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAA  >  CP043523/1890966‑1891200

gTAAGAGGGCATTACAACGGCGGACAAAGCGAGAATATTCTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAACAGTCAGATGt                                                                                         <  1:1070204/148‑1 (MQ=1)

gTAAGAGGGCATTACAACGGCGGACAAAGCGAGAATATTCTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAACAGTCAGATGt                                                                                         <  1:2533503/148‑1 (MQ=1)

gTAAGAGGGCATTACAACGGCGGACAAAGCGAGAATATTCTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAACAGTCAGATGt                                                                                         <  1:4600089/148‑1 (MQ=11)

 taggagtgCATTACAACGATGAACAAAGCGAGAATATTCTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAACAGTCAGATGtt                                                                                        >  2:488258/8‑148 (MQ=1)

                                    atgctgggagattgggaaatAAACTGTGAGCCAATTGGTGCTTAGCTGTAAGCGGTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAACAGGCAGATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAg                                                     <  2:3544458/142‑1 (MQ=1)

                                       cTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGa                                                  <  1:1050517/148‑1 (MQ=1)

                                       cTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGa                                                  >  2:2370204/1‑148 (MQ=11)

                                       cTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGa                                                  <  1:4016148/148‑1 (MQ=11)

                                       cTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGa                                                  <  1:3857283/148‑1 (MQ=11)

                                        taggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaa                                                 <  2:1110401/148‑1 (MQ=14)

                                        taggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaa                                                 <  2:4391182/148‑1 (MQ=14)

                                        taggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaa                                                 <  1:4344021/148‑1 (MQ=14)

                                        taggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaa                                                 >  1:2220194/1‑148 (MQ=14)

                                        taggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaa                                                 <  2:698527/148‑1 (MQ=14)

                                         aggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaaa                                                >  1:2816688/1‑148 (MQ=17)

                                         aggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaaa                                                >  1:1288102/1‑148 (MQ=17)

                                         aggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaaa                                                >  2:2925739/1‑148 (MQ=17)

                                         aggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGaaa                                                >  1:4105635/1‑148 (MQ=17)

                                          ggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAac                                               <  2:1742129/148‑1 (MQ=17)

                                          ggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAac                                               >  2:3549588/1‑148 (MQ=17)

                                          ggaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAAAGAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAac                                               <  1:257390/148‑1 (MQ=255)

                                           gaaattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAaca                                              <  1:1127443/148‑1 (MQ=17)

                                             aattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACt                                            >  1:4208374/1‑148 (MQ=17)

                                             aattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACt                                            >  1:4485578/1‑148 (MQ=17)

                                             aattaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACt                                            >  2:3331520/1‑148 (MQ=17)

                                              attaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTc                                           >  2:783179/1‑148 (MQ=17)

                                               ttaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCg                                          >  2:2520687/1‑148 (MQ=17)

                                               ttaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCg                                          >  2:8810/1‑148 (MQ=17)

                                               ttaggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCg                                          <  1:2087099/148‑1 (MQ=17)

                                                taggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGt                                         >  2:2063754/1‑148 (MQ=17)

                                                taggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGt                                         >  2:593764/1‑148 (MQ=17)

                                                 aggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGtt                                        <  2:2598738/148‑1 (MQ=17)

                                                  ggaaatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGttt                                       <  2:3828272/148‑1 (MQ=18)

                                                     aatAACCTTGGAGCCAATTGGGGCTTGGCTTTAAGCGTTTCACTAAAATGCAGTCCGCCCCATAGTTAACAAGCGATAACAAAGCAATCTGATGTTGCGAGATGGAGGTTCTTTCGGTTTTGACCCTCAAGGGAACCCCTCGTTTGAt                                    <  2:2318547/148‑1 (MQ=11)

                                                     aatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGAt                                    >  1:3080358/1‑148 (MQ=17)

                                                     aatAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGAt                                    >  1:1588139/1‑148 (MQ=17)

                                                      atAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATa                                   >  2:133476/1‑148 (MQ=17)

                                                        aaaCTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATaaa                                 <  1:1567618/148‑1 (MQ=17)

                                                          aCTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACCCTCGTTTGATAAAGc                               <  2:4140408/148‑1 (MQ=12)

                                                          aCTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGc                               >  2:4574048/1‑148 (MQ=17)

                                                           cTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCg                              <  2:1696330/148‑1 (MQ=17)

                                                           cTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCg                              >  1:2518753/1‑148 (MQ=17)

                                                            tttGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGt                             >  1:173631/1‑148 (MQ=17)

                                                            tttGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGt                             <  1:3742523/148‑1 (MQ=17)

                                                            tttGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGt                             >  2:3960084/1‑148 (MQ=17)

                                                            tttGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGCAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGt                             >  2:1365762/1‑148 (MQ=18)

                                                             ttGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtc                            >  2:3301906/1‑148 (MQ=17)

                                                             ttGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtc                            <  1:1402760/148‑1 (MQ=17)

                                                             ttGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCGGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtc                            >  1:2704550/1‑148 (MQ=18)

                                                              tGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtct                           <  2:1468324/148‑1 (MQ=17)

                                                              tGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtct                           >  1:1095844/1‑148 (MQ=17)

                                                              tGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtct                           <  2:3639421/148‑1 (MQ=17)

                                                               gAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtctc                          <  2:1899723/148‑1 (MQ=17)

                                                               gAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtctc                          <  1:2759526/148‑1 (MQ=17)

                                                               gAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGACGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGtctc                          <  2:1322060/148‑1 (MQ=17)

                                                                aGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCa                         >  2:346729/1‑148 (MQ=17)

                                                                aGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCa                         <  1:756774/148‑1 (MQ=17)

                                                                aGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCa                         <  1:4214943/148‑1 (MQ=17)

                                                                  ccAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAAt                       <  1:994387/148‑1 (MQ=17)

                                                                    aaTTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATgg                     <  2:2906750/148‑1 (MQ=17)

                                                                    aaTTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATgg                     >  1:3553498/1‑148 (MQ=17)

                                                                     aTTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGt                    <  1:1446275/148‑1 (MQ=17)

                                                                     aTTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGt                    >  1:3214548/1‑148 (MQ=17)

                                                                      ttGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGtt                   <  2:2837715/148‑1 (MQ=17)

                                                                       tGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGtta                  >  1:1201193/1‑147 (MQ=17)

                                                                       tGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGtta                  <  1:1971619/148‑2 (MQ=17)

                                                                           gCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAg              >  2:301957/1‑148 (MQ=12)

                                                                           gCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAg              <  2:3083055/148‑1 (MQ=12)

                                                                           gCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAg              >  1:976045/1‑148 (MQ=12)

                                                                           gCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAg              <  1:1579908/148‑1 (MQ=12)

                                                                           gCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAg              <  1:3408806/148‑1 (MQ=12)

                                                                            cTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGt             <  2:991212/148‑1 (MQ=12)

                                                                            cTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGt             <  1:360375/148‑1 (MQ=12)

                                                                             ttAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGta            <  2:1392578/148‑1 (MQ=12)

                                                                             ttAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGta            <  2:1209713/148‑1 (MQ=17)

                                                                             ttAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGta            <  1:2962426/148‑1 (MQ=12)

                                                                             ttAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGta            >  2:3884557/1‑148 (MQ=12)

                                                                              tAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaa           >  1:2384609/1‑148 (MQ=17)

                                                                               aGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaa          >  1:1106422/1‑148 (MQ=12)

                                                                               aGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaa          <  2:4249642/148‑1 (MQ=17)

                                                                               aGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaa          >  1:3172687/1‑148 (MQ=12)

                                                                                gCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATCAAGCGTCTCAATGGTTATCAGtaaat         <  2:4577805/148‑1 (MQ=12)

                                                                                gCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaat         <  1:2289207/148‑1 (MQ=12)

                                                                                 cTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaata        >  1:3319572/1‑148 (MQ=12)

                                                                                 cTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaata        <  1:2897503/148‑1 (MQ=12)

                                                                                 cTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaata        <  2:640808/148‑1 (MQ=12)

                                                                                 cTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaata        <  1:3769313/148‑1 (MQ=12)

                                                                                 cTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaata        <  1:675886/148‑1 (MQ=12)

                                                                                 cTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaata        >  2:1804608/1‑148 (MQ=17)

                                                                                  tttAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaataa       >  1:2911629/1‑148 (MQ=12)

                                                                                  tttAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaataa       >  1:2843079/1‑148 (MQ=17)

                                                                                   ttAAGCGTTTTCCTCAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGGGTTGTCCGTTCTTTCGGTTTTGTACCTCAAGGGAAACACTCGTTTGATAAATCGACTCATTGGTTATCTGtagataaa      >  2:4229502/1‑148 (MQ=11)

                                                                                   ttAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGAGGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGtaaataaa      <  1:4134511/148‑1 (MQ=255)

                                                                                   ttAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAACAGTCAGATGTTGCAAGATGGACGTTCTTTCGGTTTTGAACCTCAAGAGAAACAATCGTTTGATAAAGCGTCTCAATGGTTGTCAGtaaataaa      <  2:1850009/148‑1 (MQ=11)

                                                                                    tAAGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGTAAATAAAc     >  2:2416171/1‑148 (MQ=17)

                                                                                     aaGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGTAAATAAACa    >  1:2397547/1‑148 (MQ=12)

                                                                                     aaGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGTAAATAAACa    >  2:1709332/1‑148 (MQ=12)

                                                                                      aGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGNACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCNATGGTTATCAGTAAATAAACaa   <  1:482305/148‑1 (MQ=9)

                                                                                      aGCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGTAAATAAACaa   <  1:2973460/148‑1 (MQ=12)

                                                                                       gCGTTTCACTAAAATGCAGTACGCCCCATAGGTAACAAGCGATAACTAAGAAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTATCAGTAAATAAACaaa  <  1:1360279/148‑1 (MQ=9)

GTAAGAGGGCATTACAACGGCGGACAAAGCGAGAATATTCTAGGAAATTAGGAAATAAACTTTGAGCCAATTGGTGCTTAGCTTTAAGCGTTTCACTAAAATGCAGTACGCCCCATAGATAACAAGCGATAACTAAGCAATCTGATGTTGCGAGATGGACGTTCTTTCGGTTTTGAACCTCAAGGGAAACACTCGTTTGATAAAGCGTCTCAATGGTTGTCAGTAAATAAACAAA  >  CP043523/1890966‑1891200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`