BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,895,466	T→C	L196L (TTA→CTA)	FTN78_chr1944 →	Mobile element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,895,466	0	T	C	76.4%	1206.0 / inf	1123	L196L (TTA→CTA)	FTN78_chr1944	Mobile element protein
Reads supporting (aligned to +/- strand): ref base T (115/150); new base C (387/469); total (504/619)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 1133 total aligned reads displayed.

GGATTTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGACAA  >  CP043523/1895322‑1895613

ggctgtAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAg                                                                                                                                                  <  2:1089821/143‑1 (MQ=0)

ggctgtAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAg                                                                                                                                                  <  2:4198901/143‑1 (MQ=0)

                                               cTACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATg                                                                                                   <  2:740889/148‑1 (MQ=2)

                                                tACACAATTACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGa                                                                                                  <  2:3588631/148‑1 (MQ=9)

                                                tACACAATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGa                                                                                                  >  1:1519108/1‑148 (MQ=14)

                                                  cacaATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGaca                                                                                                >  1:3989159/1‑148 (MQ=17)

                                                   acaATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGacac                                                                                               <  2:3298534/148‑1 (MQ=17)

                                                    tgaTTAGTCCTGCTTCAATAGCTGACAGTATAATGGCCGAGGAAGTATTGTCTCAATTTGGCACACCCATAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACAcc                                                                                              >  1:4230797/3‑148 (MQ=11)

                                                       ttACTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGt                                                                                           <  1:1763206/148‑1 (MQ=12)

                                                         aCTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCa                                                                                         >  1:1273029/1‑148 (MQ=17)

                                                         aCTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCa                                                                                         <  1:3319081/148‑1 (MQ=17)

                                                          cTCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAg                                                                                        <  1:4367977/148‑1 (MQ=17)

                                                           tCCTGCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAgg                                                                                       >  1:2306901/1‑148 (MQ=12)

                                                           tCCTGCTTCAATGGCTGAGCGGTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAgg                                                                                       <  2:3791159/148‑1 (MQ=255)

                                                               gCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGgaaga                                                                                   <  2:3141195/148‑1 (MQ=12)

                                                               gCTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGgaaga                                                                                   >  1:1381472/1‑148 (MQ=17)

                                                               gCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGgaaga                                                                                   <  2:1906825/148‑1 (MQ=17)

                                                                cTTCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGgaagaa                                                                                  >  2:1010121/1‑148 (MQ=12)

                                                                 ttCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAAc                                                                                 <  1:223541/148‑1 (MQ=17)

                                                                 ttCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAAc                                                                                 >  2:4080834/1‑148 (MQ=12)

                                                                  tCAATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACa                                                                                >  1:758265/1‑148 (MQ=12)

                                                                    aaTGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATg                                                                              <  2:2801522/148‑1 (MQ=12)

                                                                    aaTGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATg                                                                              <  2:1205719/148‑1 (MQ=17)

                                                                     aTGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGa                                                                             <  1:3251326/148‑1 (MQ=17)

                                                                      tGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGaa                                                                            <  2:2445751/148‑1 (MQ=12)

                                                                      tGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGaa                                                                            <  2:2720190/148‑1 (MQ=17)

                                                                       ggCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAg                                                                           <  1:674439/148‑1 (MQ=17)

                                                                       ggCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAg                                                                           <  1:2422595/148‑1 (MQ=12)

                                                                        gCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGc                                                                          <  1:1021456/148‑1 (MQ=12)

                                                                         cTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCa                                                                         >  1:930817/1‑148 (MQ=12)

                                                                         cTGATAGTTCAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCa                                                                         <  2:3078823/148‑1 (MQ=17)

                                                                          tgatctttCAATGACCGAGGAATTGTTTAGTCAATTTGGTACCCCAACAGTCCTTTGAGATATGGGATATCTAGGTCCGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCaa                                                                        <  2:3671829/142‑1 (MQ=11)

                                                                          tGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCaa                                                                        <  1:3758135/148‑1 (MQ=12)

                                                                           gatcgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCaaa                                                                       <  1:614371/144‑1 (MQ=12)

                                                                            atcgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCaaaa                                                                      >  2:2201322/4‑148 (MQ=17)

                                                                            atcgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCaaaa                                                                      <  1:1952769/145‑1 (MQ=17)

                                                                             tcgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAg                                                                     <  1:1072035/146‑1 (MQ=17)

                                                                              cgTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGa                                                                    <  2:1819629/147‑1 (MQ=17)

                                                                                ttCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGaaa                                                                  <  1:831773/148‑1 (MQ=17)

                                                                                 tCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGaaaa                                                                 >  1:2134920/1‑148 (MQ=17)

                                                                                  cAATGACCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAAt                                                                <  1:4458128/148‑1 (MQ=17)

                                                                                  cAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAAt                                                                <  2:2911388/148‑1 (MQ=17)

                                                                                   aaTGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATc                                                               >  2:3476774/1‑148 (MQ=17)

                                                                                    aTGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATcc                                                              >  2:1050889/1‑148 (MQ=17)

                                                                                    aTGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATcc                                                              <  1:3583834/148‑1 (MQ=17)

                                                                                     tGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCt                                                             >  1:3104648/1‑148 (MQ=17)

                                                                                       aCCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCttt                                                           >  2:14765/1‑148 (MQ=17)

                                                                                       aCCGAGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCttt                                                           >  1:2968606/1‑148 (MQ=17)

                                                                                         cGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTc                                                         <  2:4404109/148‑1 (MQ=17)

                                                                                           aGGAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTccc                                                       <  2:2561337/148‑1 (MQ=17)

                                                                                            ggAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCt                                                      <  2:4293348/148‑1 (MQ=17)

                                                                                            ggAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCt                                                      <  1:4158474/148‑1 (MQ=17)

                                                                                             gAAGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTa                                                     >  1:2644744/1‑148 (MQ=17)

                                                                                              aaGTGTTGAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTaa                                                    >  2:342374/1‑148 (MQ=17)

                                                                                                gtgtTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAtt                                                  <  2:4601736/148‑1 (MQ=17)

                                                                                                 tgtTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAttt                                                 <  1:2848515/148‑1 (MQ=17)

                                                                                                   ttGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAAttttt                                               >  2:1320966/1‑148 (MQ=17)

                                                                                                    tGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTc                                              >  2:3974265/1‑148 (MQ=17)

                                                                                                    tGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTc                                              >  1:1704276/1‑148 (MQ=17)

                                                                                                     gAGTCTATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGATCATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCa                                             <  2:4712308/148‑1 (MQ=17)

                                                                                                     gAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCa                                             <  2:786577/148‑1 (MQ=17)

                                                                                                      aGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCaa                                            <  2:1539200/148‑1 (MQ=17)

                                                                                                       gTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCaaa                                           >  1:1822165/1‑148 (MQ=17)

                                                                                                         cAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAAc                                         >  1:4076764/1‑148 (MQ=17)

                                                                                                          aaTTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACg                                        >  2:2091310/1‑148 (MQ=17)

                                                                                                            tttGGGACACCAACAGTCCTTGGGGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTa                                      <  1:2174582/148‑1 (MQ=17)

                                                                                                            tttGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTa                                      <  1:3668958/148‑1 (MQ=17)

                                                                                                            tttGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTa                                      >  1:2480160/1‑148 (MQ=17)

                                                                                                             ttGGGACACCAGCAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGTAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAg                                     <  1:519310/148‑1 (MQ=12)

                                                                                                              tGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGa                                    >  1:2763169/1‑148 (MQ=32)

                                                                                                              tGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGa                                    <  2:3230921/148‑1 (MQ=17)

                                                                                                              tGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGa                                    >  1:1864643/1‑148 (MQ=17)

                                                                                                                ggACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGaaa                                  >  2:827205/1‑148 (MQ=17)

                                                                                                                 gACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGaaaa                                 <  2:2505881/148‑1 (MQ=17)

                                                                                                                  acacCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAg                                >  2:1748533/1‑148 (MQ=17)

                                                                                                                   cacCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGt                               >  1:3808079/1‑148 (MQ=17)

                                                                                                                   cacCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGt                               >  1:3893362/1‑148 (MQ=17)

                                                                                                                    acCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTg                              >  1:3532813/1‑148 (MQ=17)

                                                                                                                     ccAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGa                             >  1:3423731/1‑148 (MQ=32)

                                                                                                                       aaCAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGAtt                           <  1:1155675/148‑1 (MQ=17)

                                                                                                                        aCAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTg                          <  2:3849988/148‑1 (MQ=32)

                                                                                                                         cAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGa                         <  1:304128/148‑1 (MQ=17)

                                                                                                                          aGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAg                        >  1:2098385/1‑148 (MQ=17)

                                                                                                                            tCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCg                      >  1:4572542/1‑148 (MQ=17)

                                                                                                                            tCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCg                      >  2:2378554/1‑148 (MQ=17)

                                                                                                                              cTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCGAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAg                    <  1:4638867/148‑1 (MQ=12)

                                                                                                                              cTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAg                    >  2:607151/1‑148 (MQ=17)

                                                                                                                               ttGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGt                   >  2:2298256/1‑148 (MQ=32)

                                                                                                                                 ggAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGttt                 >  1:2567769/1‑148 (MQ=32)

                                                                                                                                 ggAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGttt                 >  2:1992696/1‑148 (MQ=17)

                                                                                                                                 ggAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGttt                 <  2:547220/148‑1 (MQ=17)

                                                                                                                                   agaTATGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTtc               <  1:4308842/148‑1 (MQ=17)

                                                                                                                                    gaTATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTtct              <  1:145687/148‑1 (MQ=32)

                                                                                                                                      tatGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTctct            <  2:905304/148‑1 (MQ=32)

                                                                                                                                       atGGGATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCtt           <  2:4476556/148‑1 (MQ=17)

                                                                                                                                         gggATATCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCtttt         >  2:297304/1‑148 (MQ=17)

                                                                                                                                            atatTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTg      <  1:2000988/148‑1 (MQ=32)

                                                                                                                                            atatCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTg      <  2:463587/148‑1 (MQ=17)

                                                                                                                                            atatCTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTg      <  2:1604552/148‑1 (MQ=17)

                                                                                                                                               tttAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGACa   >  1:289632/1‑148 (MQ=32)

GGATTTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCAATGGCTGATAGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATTTAGGTCAGTCACTGCATGATAGGCTGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGAGCGAGTTTTCTCTTTTTTGACAA  >  CP043523/1895322‑1895613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`