BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,848,417	T→C	D42D (GAT→GAC)	FTN78_chr1898 →	Cold shock protein of CSP family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,848,417	0	T	C	99.4%	925.6 / ‑3.7	331	D42D (GAT→GAC)	FTN78_chr1898	Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (163/166); total (163/168)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 333 total aligned reads displayed.

CGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAGCTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAATGATGA  >  CP043523/1848321‑1848561

cgctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCaa                                                                                               <  2:2535275/142‑1 (MQ=11)

cgctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCaa                                                                                               <  1:3532205/142‑1 (MQ=11)

cgctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCaa                                                                                               >  2:3335242/7‑148 (MQ=11)

 gctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAg                                                                                              <  2:3229883/143‑1 (MQ=11)

  ctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGc                                                                                             >  1:1705219/5‑148 (MQ=11)

   tactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCa                                                                                            >  1:2105780/4‑148 (MQ=11)

   tacgaaaGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCa                                                                                            <  2:4120615/144‑1 (MQ=11)

    actAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAg                                                                                           >  1:3344408/3‑148 (MQ=11)

    actAAAGGACTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAg                                                                                           <  1:3249596/146‑1 (MQ=11)

      tAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGtt                                                                                         >  1:2879342/1‑148 (MQ=11)

       aaaGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTa                                                                                        <  1:4099473/148‑1 (MQ=11)

       aaaGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTa                                                                                        >  2:3836472/1‑148 (MQ=11)

        aaGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTaa                                                                                       >  1:666752/1‑148 (MQ=11)

         aGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAat                                                                                      <  1:308935/148‑1 (MQ=11)

         aGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAat                                                                                      >  1:2892874/1‑148 (MQ=11)

         aGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAat                                                                                      >  1:1363225/1‑148 (MQ=11)

         aGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAat                                                                                      >  2:2514677/1‑148 (MQ=11)

          ggATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAata                                                                                     <  1:4600159/148‑1 (MQ=11)

          ggATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAata                                                                                     <  1:2786747/148‑1 (MQ=11)

          ggATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAata                                                                                     <  1:4190761/148‑1 (MQ=11)

            aTTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATc                                                                                   <  2:2478151/148‑1 (MQ=11)

             tttGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATca                                                                                  <  2:3142932/148‑1 (MQ=11)

                ggttttATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTCATATCACaa                                                                               >  2:666201/4‑148 (MQ=11)

                 gttttATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACaaa                                                                              >  1:1520755/3‑148 (MQ=11)

                          cctctgaagatGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAAtt                                                                     <  2:2210587/143‑1 (MQ=11)

                                  gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGa                                                             <  1:4433567/141‑1 (MQ=14)

                                  gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGa                                                             >  2:901718/8‑148 (MQ=14)

                                  gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGa                                                             >  2:1673591/8‑148 (MQ=14)

                                  gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGa                                                             >  2:4019664/8‑148 (MQ=14)

                                     ggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGaaaa                                                          >  2:4423595/5‑148 (MQ=255)

                                     ggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGaaaa                                                          <  1:3992256/144‑1 (MQ=14)

                                      gtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAAt                                                         >  2:1159299/4‑148 (MQ=14)

                                       tcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATc                                                        >  2:3554169/3‑148 (MQ=255)

                                       tcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATc                                                        >  2:3547149/3‑148 (MQ=255)

                                       tcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATc                                                        >  2:3480563/3‑148 (MQ=255)

                                       tcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATc                                                        >  2:2905991/3‑148 (MQ=255)

                                        caaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCg                                                       >  2:82244/2‑148 (MQ=255)

                                         aaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGt                                                      >  2:3108856/1‑148 (MQ=255)

                                           gATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGc                                                    >  1:184379/1‑148 (MQ=255)

                                           gATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGc                                                    <  2:3769553/148‑1 (MQ=255)

                                           gATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGc                                                    >  2:1018993/1‑148 (MQ=255)

                                            aTTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCa                                                   <  1:1641260/148‑1 (MQ=255)

                                             tttGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaa                                                  <  1:4331024/148‑1 (MQ=255)

                                             tttGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaa                                                  <  2:2292684/148‑1 (MQ=255)

                                              ttGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAATCACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaa                                                 <  1:2273691/148‑1 (MQ=255)

                                               tGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaaa                                                <  1:3903381/148‑1 (MQ=255)

                                               tGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaaa                                                >  2:4651015/1‑148 (MQ=255)

                                               tGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaaa                                                <  1:3675250/148‑1 (MQ=255)

                                                 ttCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaacac                                              >  1:3575853/1‑145 (MQ=255)

                                                 ttCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaaaac                                              >  2:1784445/1‑146 (MQ=255)

                                                  tCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACt                                             <  2:2407566/148‑1 (MQ=255)

                                                     cTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGa                                          <  1:1711244/148‑1 (MQ=14)

                                                     cTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGa                                          >  1:1016397/1‑148 (MQ=14)

                                                     cTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGa                                          <  2:4332628/148‑1 (MQ=14)

                                                        aCTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTc                                       >  1:4665927/1‑148 (MQ=14)

                                                         cTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCa                                      <  1:4580368/148‑1 (MQ=14)

                                                           tctcATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATg                                    <  2:1927988/148‑1 (MQ=14)

                                                             tcatcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGAc                                  >  1:3789314/5‑148 (MQ=17)

                                                             tcatcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGAc                                  >  1:2589572/5‑148 (MQ=14)

                                                               atcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGCAAATCGTGCAAAAACTGGAGTCATGACtt                                >  2:4174652/3‑148 (MQ=17)

                                                               atcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACtt                                <  2:2096044/146‑1 (MQ=255)

                                                               atcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACtt                                <  1:1156750/146‑1 (MQ=255)

                                                               atcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACtt                                >  1:516820/3‑148 (MQ=255)

                                                               atcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATAGTGCAAAAACTGGAGTCATGACtt                                <  2:748499/146‑1 (MQ=17)

                                                                tcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATAAAAAAAAAACTGGAGTCATGACTTc                               <  2:131794/147‑1 (MQ=14)

                                                                 caatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAGAATCGTGCAAAAACTGGAGTCATGACTTCa                              >  2:2779891/1‑148 (MQ=17)

                                                                 caatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCa                              <  1:875029/148‑1 (MQ=255)

                                                                   atccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGt                            <  1:1294334/148‑1 (MQ=255)

                                                                     ccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttt                          <  1:3734257/148‑1 (MQ=255)

                                                                       aatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttt                        >  2:2599245/1‑148 (MQ=255)

                                                                        atcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGtttttt                       <  1:2833499/148‑1 (MQ=255)

                                                                        atcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGtttttt                       <  2:2223679/148‑1 (MQ=255)

                                                                         tcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttttt                      >  1:2202950/1‑148 (MQ=255)

                                                                            gATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTtata                   <  2:2892874/148‑1 (MQ=255)

                                                                             aTGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTatat                  >  2:1763471/1‑148 (MQ=255)

                                                                              tGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAtt                 <  2:4309294/148‑1 (MQ=255)

                                                                              tGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAtt                 <  2:582318/148‑1 (MQ=255)

                                                                              tGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAtt                 >  1:3388801/1‑148 (MQ=255)

                                                                               ggATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTTTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTc                <  2:2811307/148‑1 (MQ=255)

                                                                                gATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCa               >  1:4289208/1‑148 (MQ=255)

                                                                                gATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCa               <  2:533871/148‑1 (MQ=255)

                                                                                gATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCa               >  2:2433973/1‑148 (MQ=255)

                                                                                 aTTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg              <  1:1084508/148‑1 (MQ=255)

                                                                                 aTTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg              >  2:1063828/1‑148 (MQ=255)

                                                                                 aTTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg              <  2:3711615/148‑1 (MQ=255)

                                                                                 aTTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg              <  2:482840/148‑1 (MQ=255)

                                                                                 aTTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg              <  2:4545510/148‑1 (MQ=255)

                                                                                   tCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGtt            >  1:1897857/1‑148 (MQ=255)

                                                                                   tCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGtt            >  2:3413640/1‑148 (MQ=255)

                                                                                    cAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTg           <  1:24319/148‑1 (MQ=255)

                                                                                    cAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTg           >  2:306314/1‑148 (MQ=255)

                                                                                    cAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTg           <  1:4513118/148‑1 (MQ=255)

                                                                                      aatcacTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCa         <  1:2525832/145‑1 (MQ=255)

                                                                                      aatcacTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCa         <  2:1520755/145‑1 (MQ=255)

                                                                                       atcacTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCaa        >  1:3436416/3‑148 (MQ=255)

                                                                                        tcacTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAat       >  1:248116/2‑148 (MQ=255)

                                                                                         cacTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAatt      <  2:3912951/148‑2 (MQ=255)

                                                                                          acTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAatta     <  1:1973860/148‑3 (MQ=255)

                                                                                          acTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAatta     <  2:142342/148‑3 (MQ=255)

                                                                                            ttGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAattatg   >  1:93029/1‑148 (MQ=255)

CGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAGCTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAATGATGA  >  CP043523/1848321‑1848561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`