Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,867,663 T→C L47L (TTA→CTA)  FTN78_chr1914 → Site‑specific recombinase, DNA invertase Pin related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,867,6630TC98.9% 1280.8 / NA 357L47L (TTA→CTA) FTN78_chr1914Site‑specific recombinase, DNA invertase Pin related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (152/201);  minor base A (0/4);  total (152/205)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 366 total aligned reads displayed.

TCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTTATATATTAAATATTGGACT  >  CP043523/1867518‑1867805
                                                                                                                                                 |                                                                                                                                              
tCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAatttaatcta                                                                                                                                              <  2:1758956/148‑1 (MQ=255)
tCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAatttaatcta                                                                                                                                              <  2:3512415/148‑1 (MQ=255)
   aaaTATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGta                                                                                                                                           >  1:1399465/1‑147 (MQ=255)
     atatGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtatt                                                                                                                                         >  2:4630943/1‑145 (MQ=255)
     atatGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtatt                                                                                                                                         <  2:3889419/148‑4 (MQ=255)
      tatGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtattt                                                                                                                                        >  2:4569194/1‑144 (MQ=255)
       atGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGtattta                                                                                                                                       <  1:4252892/148‑6 (MQ=255)
              ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGtt                                                                                                                                >  1:2641464/1‑148 (MQ=255)
                 ggTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaa                                                                                                                             <  2:330255/148‑2 (MQ=255)
                  gTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaag                                                                                                                            >  1:521718/1‑146 (MQ=255)
                  gTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaag                                                                                                                            >  1:1643796/1‑146 (MQ=255)
                   tATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaagg                                                                                                                           <  1:3029691/148‑4 (MQ=255)
                    aTGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaagga                                                                                                                          <  1:4094616/148‑5 (MQ=255)
                     tGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggat                                                                                                                         <  1:158681/148‑6 (MQ=255)
                     tGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggat                                                                                                                         <  2:789316/148‑6 (MQ=255)
                      gCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggatg                                                                                                                        >  1:3332465/1‑142 (MQ=255)
                      gCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggatg                                                                                                                        <  1:2336863/148‑7 (MQ=255)
                                gTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACttt                                                                                                              >  2:3341930/1‑148 (MQ=255)
                                  aCTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTaa                                                                                                            >  1:2926441/1‑148 (MQ=255)
                                    ttttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAAtt                                                                                                          <  1:3232285/148‑1 (MQ=255)
                                    ttttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAAtt                                                                                                          >  2:1711341/1‑148 (MQ=255)
                                     tttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                                                         >  1:1531081/1‑148 (MQ=255)
                                     tttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                                                         <  1:288859/148‑1 (MQ=255)
                                     tttGAACAAAAATTAGAATCTCAAATTGAAGGTCGGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                                                         >  2:2344807/1‑148 (MQ=255)
                                      ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgt                                                                                                        <  2:2610461/148‑1 (MQ=255)
                                      ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgt                                                                                                        >  2:822271/1‑148 (MQ=255)
                                       tGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgtg                                                                                                       >  2:4085565/1‑148 (MQ=255)
                                          acaaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACa                                                                                                    <  2:1025073/144‑1 (MQ=255)
                                           caaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaa                                                                                                   <  1:2539715/145‑1 (MQ=255)
                                             aaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAg                                                                                                 <  1:1942732/147‑1 (MQ=255)
                                              aaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGt                                                                                                >  1:850340/1‑148 (MQ=255)
                                               aaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGtt                                                                                               >  2:3702351/1‑148 (MQ=255)
                                                aTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTa                                                                                              <  2:3425644/148‑1 (MQ=255)
                                                  tAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGa                                                                                            <  1:3672566/148‑1 (MQ=255)
                                                   aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGac                                                                                           <  1:3836720/148‑2 (MQ=255)
                                                   aGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGac                                                                                           >  2:4378150/1‑147 (MQ=255)
                                                    gAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGacc                                                                                          <  2:36692/148‑3 (MQ=255)
                                                     aaTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGAACg                                                                                         >  2:3861761/1‑148 (MQ=255)
                                                      aTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGa                                                                                        >  2:1067570/1‑148 (MQ=255)
                                                       tctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAt                                                                                       >  1:4351495/1‑148 (MQ=255)
                                                          cAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAACAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAg                                                                                    <  1:1867425/148‑1 (MQ=255)
                                                           aaaTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGc                                                                                   <  2:1608189/148‑1 (MQ=255)
                                                             aTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCa                                                                                 >  1:4474298/1‑148 (MQ=255)
                                                              ttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAg                                                                                >  1:974972/1‑148 (MQ=255)
                                                                  aGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAac                                                                            <  1:3879826/148‑1 (MQ=255)
                                                                  aGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAAAAAAAAAAAAAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAac                                                                            <  2:2478260/148‑1 (MQ=255)
                                                                    ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGGAAAGTTTAGTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                                                          <  1:4217264/148‑1 (MQ=255)
                                                                    ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                                                          <  1:2193323/148‑1 (MQ=255)
                                                                    ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                                                          <  2:453202/148‑1 (MQ=255)
                                                                    ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                                                          <  2:312100/148‑1 (MQ=255)
                                                                        gAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                                                      <  2:3079320/148‑3 (MQ=255)
                                                                          aagaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgaga                                                                    <  2:1871079/148‑1 (MQ=255)
                                                                             aagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGt                                                                 <  2:2005215/148‑1 (MQ=255)
                                                                              agCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGtt                                                                >  2:3227684/1‑148 (MQ=255)
                                                                              agCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGtt                                                                <  2:281373/148‑1 (MQ=255)
                                                                                  ggAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAg                                                            >  1:651154/1‑148 (MQ=255)
                                                                                    aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaa                                                          >  1:3447490/1‑148 (MQ=255)
                                                                                      cAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAt                                                        <  2:285614/148‑1 (MQ=255)
                                                                                       agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAtt                                                       <  1:4591840/148‑1 (MQ=255)
                                                                                         aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                                                     >  1:2639845/1‑148 (MQ=255)
                                                                                          agaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGtt                                                    <  2:4043009/148‑1 (MQ=255)
                                                                                          agaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGtt                                                    <  1:4113640/148‑1 (MQ=255)
                                                                                           gaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                                                   >  2:1921623/1‑148 (MQ=255)
                                                                                            aagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCa                                                  >  2:1230731/1‑148 (MQ=255)
                                                                                              gTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAAt                                                >  1:1714253/1‑148 (MQ=255)
                                                                                                tttCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCg                                              >  2:2430449/1‑148 (MQ=255)
                                                                                                 ttCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGt                                             >  2:498606/1‑148 (MQ=255)
                                                                                                 ttCCAAGAAAAGTTTACTGGAAATACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGt                                             <  2:2084159/148‑1 (MQ=255)
                                                                                                   ccAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTa                                           >  2:590191/1‑148 (MQ=255)
                                                                                                      aGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTAttt                                        <  1:2123295/148‑1 (MQ=255)
                                                                                                      aGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTAttt                                        >  1:3558211/1‑148 (MQ=255)
                                                                                                       gAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTa                                       >  1:1688364/1‑148 (MQ=255)
                                                                                                       gAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTa                                       <  2:2189106/148‑1 (MQ=255)
                                                                                                        aaaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTaa                                      <  2:2254508/148‑1 (MQ=255)
                                                                                                         aaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAAt                                     <  1:2763044/148‑1 (MQ=255)
                                                                                                          aagtttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa                                    >  1:3591777/4‑148 (MQ=255)
                                                                                                          aagtttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa                                    <  1:101090/145‑1 (MQ=255)
                                                                                                          aagtttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa                                    >  1:227181/4‑148 (MQ=255)
                                                                                                                actggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGaa                              <  2:390736/142‑1 (MQ=255)
                                                                                                                 ctggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAt                             <  1:1095332/143‑1 (MQ=255)
                                                                                                                 ctggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAt                             <  2:1399465/143‑1 (MQ=255)
                                                                                                                        tACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGtt                      <  1:1161952/148‑1 (MQ=255)
                                                                                                                            gtcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcata                  <  2:3333722/145‑1 (MQ=255)
                                                                                                                            gtcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcata                  <  1:1029303/145‑1 (MQ=255)
                                                                                                                            gtcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcata                  >  1:1991739/4‑148 (MQ=255)
                                                                                                                              cgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcatata                >  1:718511/2‑148 (MQ=255)
                                                                                                                              cgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcatata                <  2:450070/147‑1 (MQ=255)
                                                                                                                               gAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCatatat               <  2:4291970/148‑1 (MQ=255)
                                                                                                                               gAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCatatat               <  2:2641464/148‑1 (MQ=255)
                                                                                                                                 aCGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGTAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTa             <  2:713846/148‑1 (MQ=255)
                                                                                                                                 aCGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTa             >  2:3644170/1‑148 (MQ=255)
                                                                                                                                 aCGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTa             >  1:3972800/1‑148 (MQ=255)
                                                                                                                                  cGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTaa            <  2:1505641/148‑1 (MQ=255)
                                                                                                                                     ccACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAata         >  1:1842481/1‑148 (MQ=255)
                                                                                                                                     ccACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAata         <  1:3122861/148‑1 (MQ=255)
                                                                                                                                      cacaATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAatat        <  1:1528809/148‑1 (MQ=255)
                                                                                                                                        caATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTg      >  1:2699939/1‑148 (MQ=255)
                                                                                                                                        caATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTg      >  1:3522228/1‑148 (MQ=255)
                                                                                                                                         aatttaatctAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTgg     <  2:3485739/148‑1 (MQ=255)
                                                                                                                                          atttaatctaGTATTTAAAAAGTTAAAGGATGGTGACACATTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATAGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTggt    >  2:2752170/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TCAAAATATGAAAATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTTATATATTAAATATTGGACT  >  CP043523/1867518‑1867805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑