Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,867,671 C→T L49F (CTC→TTT)  FTN78_chr1914 → Site‑specific recombinase, DNA invertase Pin related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,867,6710CT99.7% 1158.7 / NA 338L49F (CTC→TTT) FTN78_chr1914Site‑specific recombinase, DNA invertase Pin related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (137/200);  minor base A (0/1);  total (137/201)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

AATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTTATATATTAAATATTGGACT  >  CP043523/1867530‑1867805
                                                                                                                                             |                                                                                                                                      
  ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCACATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGtt                                                                                                                                >  1:1916685/1‑148 (MQ=255)
  ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGtt                                                                                                                                <  2:816756/148‑1 (MQ=255)
  ttGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGtt                                                                                                                                <  2:226411/148‑1 (MQ=255)
   tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTa                                                                                                                               >  2:3886424/1‑148 (MQ=255)
   tGGGTATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTa                                                                                                                               >  1:2054059/1‑148 (MQ=255)
       tATGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaagg                                                                                                                           <  2:2800321/148‑4 (MQ=255)
         tGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggat                                                                                                                         <  1:158681/148‑6 (MQ=255)
         tGCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggat                                                                                                                         <  2:789316/148‑6 (MQ=255)
          gCACGTGTCAGTACTTTTGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTaaaggatg                                                                                                                        >  1:3332465/1‑142 (MQ=255)
                        ttttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAAtt                                                                                                          >  2:1752218/1‑148 (MQ=255)
                         tttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                                                         >  2:3991328/1‑148 (MQ=255)
                         tttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                                                         >  2:540284/1‑148 (MQ=255)
                         tttGAACAAAAATTAGAATCTCAAATTGAAGGTCGGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtg                                                                                                         >  2:2344807/1‑148 (MQ=255)
                          ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgt                                                                                                        <  2:2606750/148‑1 (MQ=255)
                          ttGAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATtgt                                                                                                        <  1:1967391/148‑1 (MQ=255)
                            gAACAAAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGa                                                                                                      >  2:3043027/1‑148 (MQ=255)
                               caaaaaTTAGAATGTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaa                                                                                                   <  1:2916447/145‑1 (MQ=255)
                               caaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaa                                                                                                   >  1:3862194/4‑148 (MQ=255)
                                aaaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACaaa                                                                                                  <  2:2056120/146‑1 (MQ=255)
                                 aaaaTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAg                                                                                                 <  1:2283806/147‑1 (MQ=255)
                                    aTTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTa                                                                                              <  2:3425644/148‑1 (MQ=255)
                                          aTCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGa                                                                                        <  1:1534219/148‑1 (MQ=255)
                                           tctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAt                                                                                       >  1:4351495/1‑148 (MQ=255)
                                           tctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCAAAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAt                                                                                       <  1:4573394/148‑1 (MQ=255)
                                            ctcAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGAtt                                                                                      >  2:670926/1‑148 (MQ=255)
                                              cAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAACAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAg                                                                                    <  1:1867425/148‑1 (MQ=255)
                                              cAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAg                                                                                    >  1:298483/1‑148 (MQ=255)
                                               aaaTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGc                                                                                   <  2:1651476/148‑1 (MQ=255)
                                               aaaTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGc                                                                                   <  2:3168146/148‑1 (MQ=255)
                                                 aTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCa                                                                                 >  1:4470567/1‑148 (MQ=255)
                                                 aTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCa                                                                                 <  1:76581/148‑1 (MQ=255)
                                                 aTTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCa                                                                                 <  1:3055536/148‑1 (MQ=255)
                                                  ttGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAg                                                                                >  1:620336/1‑148 (MQ=255)
                                                   tGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAgg                                                                               <  2:2742532/148‑1 (MQ=255)
                                                   tGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAgg                                                                               <  1:2248454/148‑1 (MQ=255)
                                                      aGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAAAAAAAAAAAAAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAac                                                                            <  2:2478260/148‑1 (MQ=255)
                                                        ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGGAAAGTTTAGTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                                                          <  1:4217264/148‑1 (MQ=255)
                                                        ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                                                          <  2:309436/148‑1 (MQ=255)
                                                        ttCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAacac                                                                          <  1:2191495/148‑1 (MQ=255)
                                                           tGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTag                                                                       <  1:3998485/148‑2 (MQ=255)
                                                            gAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                                                      >  1:2728703/1‑146 (MQ=255)
                                                            gAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTaga                                                                      <  2:4185742/148‑3 (MQ=255)
                                                             aaaGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTagag                                                                     <  2:1838398/148‑1 (MQ=255)
                                                              aagaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgaga                                                                    <  2:4382184/148‑1 (MQ=255)
                                                               agaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgagaa                                                                   <  1:3890770/148‑1 (MQ=255)
                                                               agaagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAgagaa                                                                   <  1:3667055/148‑1 (MQ=255)
                                                                 aagCAGGAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGt                                                                 <  2:2004627/148‑1 (MQ=255)
                                                                       gAGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGa                                                           >  2:379127/1‑148 (MQ=255)
                                                                        aGCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaa                                                          >  1:3447490/1‑148 (MQ=255)
                                                                         gCAGAAGAAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGaaa                                                         >  1:3238720/1‑148 (MQ=255)
                                                                           agaagaAGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAAtt                                                       <  2:2138108/148‑1 (MQ=255)
                                                                            gaagaaGTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTg                                                      >  2:3303362/1‑148 (MQ=255)
                                                                             aagaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGt                                                     >  1:2639845/1‑148 (MQ=255)
                                                                              agaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGtt                                                    <  2:4043037/148‑1 (MQ=255)
                                                                               gaagTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTc                                                   >  1:4121662/1‑148 (MQ=255)
                                                                                  gTTTTCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAAt                                                >  1:1714253/1‑148 (MQ=255)
                                                                                   ttttCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATc                                               >  1:670638/1‑148 (MQ=255)
                                                                                      tCCAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGtt                                            <  1:2564211/148‑1 (MQ=255)
                                                                                       ccAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTa                                           <  1:107897/148‑1 (MQ=255)
                                                                                       ccAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTa                                           >  2:1522492/1‑148 (MQ=255)
                                                                                       ccAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTa                                           <  2:4351495/148‑1 (MQ=255)
                                                                                       ccAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTa                                           >  2:590191/1‑148 (MQ=255)
                                                                                        cAAGAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATATAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTAt                                          <  2:3694996/148‑1 (MQ=255)
                                                                                           gAAAAGTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTGGAAATTGTTCAATCGTTATTTa                                       <  1:1668114/148‑1 (MQ=255)
                                                                                            aaaagTTTACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTaa                                      <  1:939950/148‑1 (MQ=255)
                                                                                             aaagtttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAAt                                     <  2:3377117/144‑1 (MQ=255)
                                                                                              aagtttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATa                                    <  1:101090/145‑1 (MQ=255)
                                                                                                 tttACTGGAACTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATagag                                 >  1:3799575/1‑148 (MQ=255)
                                                                                                   tactggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGATATTGTTCAATCGTTATTTAATAGAGGa                               <  1:1689574/141‑1 (MQ=255)
                                                                                                   tactggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGa                               >  1:2870471/8‑148 (MQ=255)
                                                                                                   tactggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGa                               <  2:1895908/141‑1 (MQ=255)
                                                                                                      tggaaCTACAGTCGAACGTCGTCAATTTAATCTAGGATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTTAATCGTTATTTAATAGAGGAAtt                            <  2:3648493/144‑1 (MQ=255)
                                                                                                      tggaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAAtt                            >  2:1040033/5‑148 (MQ=255)
                                                                                                        gaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTaa                          <  2:2842978/146‑1 (MQ=255)
                                                                                                        gaaCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTaa                          <  1:2525316/146‑1 (MQ=255)
                                                                                                          aCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAgg                        <  1:4630943/148‑1 (MQ=255)
                                                                                                          aCTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAgg                        <  2:1337640/148‑1 (MQ=255)
                                                                                                           cTACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGt                       <  1:4100825/148‑1 (MQ=255)
                                                                                                            tACAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGtt                      <  1:1161952/148‑1 (MQ=255)
                                                                                                             aCAGTCGAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGttc                     <  2:1434612/148‑2 (MQ=255)
                                                                                                                gtcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcata                  <  2:3333722/145‑1 (MQ=255)
                                                                                                                gtcgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcata                  <  1:1029303/145‑1 (MQ=255)
                                                                                                                  cgAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTcatata                <  2:450070/147‑1 (MQ=255)
                                                                                                                   gAACGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCatatat               <  2:2641464/148‑1 (MQ=255)
                                                                                                                    aaCGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATAtt              >  1:1339926/1‑148 (MQ=255)
                                                                                                                    aaCGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATAtt              >  1:3545050/1‑148 (MQ=255)
                                                                                                                      cGTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTaa            <  2:3576720/148‑1 (MQ=255)
                                                                                                                       gTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTaaa           <  1:2689762/148‑1 (MQ=255)
                                                                                                                       gTCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTaaa           >  2:1153357/1‑148 (MQ=255)
                                                                                                                        tCCACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAat          >  2:4556742/1‑148 (MQ=255)
                                                                                                                         ccACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAata         >  1:3586055/1‑148 (MQ=255)
                                                                                                                         ccACAATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAata         >  1:1842481/1‑148 (MQ=255)
                                                                                                                          cacaATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAatat        >  2:4603295/1‑148 (MQ=255)
                                                                                                                          cacaATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAatat        <  1:1528809/148‑1 (MQ=255)
                                                                                                                           acaATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATAtt       >  1:3131962/1‑148 (MQ=255)
                                                                                                                            caATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTg      >  1:3522228/1‑148 (MQ=255)
                                                                                                                            caATTTAATCTAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTg      <  2:1000960/148‑1 (MQ=255)
                                                                                                                             aatttaatctAGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTgg     <  2:3485739/148‑1 (MQ=255)
                                                                                                                              atttaatctaGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTggt    <  2:2456853/148‑2 (MQ=255)
                                                                                                                                ttaatctaGTATTTAAAAAGTTAAAGGATGGTGACACTTTAATTGTGACAAAGTTAGACCGATTAGCCAGGAACACTAGAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTCATATATTAAATATTGGTCt  <  2:3238720/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                      
AATTGGGTATGCACGTGTCAGTACTTTTGAACAGAAATTAGAATCTCAAATTGAAGTTCTGAAAGAAGCAGGAGCAGAAGAAGTTTTCCAAGAAAAATTTACGGGGACTACAGTTGAACGTCCACAATTTAATTTAGTGCTCAAAAAGTTAACAAATGGCGATACTTTAATTGTGACAAAGTTAGATCGATTAGCCAGGAACACTAAAGAAGTTTTAGAAATTGTTCAATCGTTATTTAATAGAGGAATTAAGGTTTATATATTAAATATTGGACT  >  CP043523/1867530‑1867805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑