Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,883,049 A→G N69S (AAT→AGT)  FTN78_chr1927 → 3‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,883,0490AG99.6% 1814.9 / NA 500N69S (AAT→AGT) FTN78_chr19273‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (253/245);  minor base T (0/2);  total (253/247)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 500 total aligned reads displayed.

ACTGGTAAAACACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAATGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCT‑TAGACTCCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGT  >  CP043523/1882904‑1883192
                                                                                                                                                 |                                                                                                                                                
  tGGTAAAACACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGcc                                                                                                                                              <  1:555407/148‑1 (MQ=255)
   ggTAAAACACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGccc                                                                                                                                             <  1:863468/148‑1 (MQ=255)
    gTAAAACACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCa                                                                                                                                            >  1:251146/1‑148 (MQ=255)
    gTAAAACACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCa                                                                                                                                            <  2:3712745/148‑1 (MQ=255)
     tAAAACACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCaa                                                                                                                                           >  1:1535933/1‑148 (MQ=255)
      aaaaCACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAAc                                                                                                                                          >  1:1234319/1‑148 (MQ=255)
      aaaaCACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAAc                                                                                                                                          >  2:3270795/1‑148 (MQ=255)
         acacAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTag                                                                                                                                       <  1:3774722/148‑1 (MQ=255)
         acacAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTag                                                                                                                                       <  1:1932023/148‑1 (MQ=255)
           acaAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTagag                                                                                                                                     >  2:4232069/1‑148 (MQ=255)
           acaAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTagag                                                                                                                                     >  2:4017830/1‑148 (MQ=255)
            caAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTAAAGAATAAAAAAAAAGGAGTGCCCAACTAGAgg                                                                                                                                    <  2:4148131/148‑1 (MQ=255)
              aaCTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGTCTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGgaa                                                                                                                                  <  2:3227513/148‑1 (MQ=255)
              aaCTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGgaa                                                                                                                                  >  1:4206588/1‑148 (MQ=255)
               aCTGAGCTCTGTAAAGATTATGGGGTATCGTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAGCTAGAGgaag                                                                                                                                 <  2:1776681/148‑1 (MQ=255)
               aCTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGgaag                                                                                                                                 >  1:607053/1‑148 (MQ=255)
               aCTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGgaag                                                                                                                                 <  1:3698605/148‑1 (MQ=255)
               aCTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGgaag                                                                                                                                 >  1:975006/1‑148 (MQ=255)
                   aaCTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGaaaa                                                                                                                             <  2:1468572/148‑1 (MQ=255)
                         gTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGAt                                                                                                                       <  1:2354546/148‑1 (MQ=255)
                         gTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGAt                                                                                                                       >  1:50548/1‑148 (MQ=255)
                          tAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATc                                                                                                                      >  1:2265027/1‑148 (MQ=255)
                           aaaGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCt                                                                                                                     >  2:1284271/1‑148 (MQ=255)
                           aaaGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCt                                                                                                                     >  2:1583506/1‑148 (MQ=255)
                             aGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTa                                                                                                                   <  1:4056323/148‑1 (MQ=255)
                             aGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTa                                                                                                                   <  1:4014587/148‑1 (MQ=255)
                             aGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTa                                                                                                                   >  2:2737847/1‑148 (MQ=255)
                             aGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTa                                                                                                                   <  1:1030056/148‑1 (MQ=255)
                             aGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTa                                                                                                                   <  1:2891661/148‑1 (MQ=255)
                              gATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTaa                                                                                                                  >  1:2410435/1‑148 (MQ=255)
                               aTTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTaaa                                                                                                                 <  2:465260/148‑1 (MQ=255)
                                ttATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTaaaa                                                                                                                >  1:2695441/1‑148 (MQ=255)
                                  aTGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTaaaaaa                                                                                                              <  1:3230646/148‑1 (MQ=255)
                                   tGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTaaaaaaa                                                                                                             >  2:2816883/1‑148 (MQ=255)
                                    ggggTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAg                                                                                                            >  1:1741268/1‑148 (MQ=255)
                                    ggggTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAg                                                                                                            <  2:750867/148‑1 (MQ=255)
                                       gTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGa                                                                                                         <  2:4413879/148‑1 (MQ=255)
                                       gTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGa                                                                                                         <  2:2665132/148‑1 (MQ=255)
                                       gTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGa                                                                                                         <  1:3958259/148‑1 (MQ=255)
                                        tATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAATAAAATCTGATCTTAAAAAAAGCGAg                                                                                                        >  1:3136950/1‑148 (MQ=255)
                                        tATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAg                                                                                                        >  2:3576862/1‑148 (MQ=255)
                                                       ttGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCa                                                                                         >  1:913495/1‑148 (MQ=255)
                                                           aaaaTGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAAc                                                                                     <  2:2324120/148‑1 (MQ=255)
                                                            aaaTGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACt                                                                                    <  1:2149355/148‑1 (MQ=255)
                                                              aTGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGAAAAAAAAAAAAGCGAGTGCCATATTCATGCAAAACTcc                                                                                  <  2:1909251/148‑1 (MQ=255)
                                                               tGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCa                                                                                 <  1:1010730/148‑1 (MQ=255)
                                                                  aTTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGt                                                                              <  1:3947163/148‑1 (MQ=255)
                                                                   ttAAGTAGTACTCTCAAGTGCGTCTTGAAGATAATACGGGGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTa                                                                             <  1:1139683/148‑1 (MQ=255)
                                                                     aaGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAg                                                                           >  1:3662506/1‑148 (MQ=255)
                                                                        cAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGAtt                                                                        >  2:1646712/1‑148 (MQ=255)
                                                                        cAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGAtt                                                                        <  1:3823286/148‑1 (MQ=255)
                                                                           tACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGCCTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCACTTAAGATTGaa                                                                     >  1:3207007/1‑148 (MQ=255)
                                                                           tACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGaa                                                                     <  1:1807731/148‑1 (MQ=255)
                                                                            aCTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGaaa                                                                    >  2:2165123/1‑148 (MQ=255)
                                                                              tctcAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGc                                                                  >  2:2931899/1‑148 (MQ=255)
                                                                               ctcAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGcc                                                                 >  2:704158/1‑148 (MQ=255)
                                                                                tcAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCa                                                                >  1:4323577/1‑148 (MQ=255)
                                                                                tcAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCa                                                                <  1:1467027/148‑1 (MQ=255)
                                                                                tcAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCa                                                                <  1:277517/148‑1 (MQ=255)
                                                                                  aaGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATc                                                              <  2:1115829/148‑1 (MQ=255)
                                                                                  aaGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATc                                                              <  1:2751500/148‑1 (MQ=255)
                                                                                   aGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCt                                                             >  1:653138/1‑148 (MQ=255)
                                                                                     tGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCtat                                                           >  2:2124797/1‑146 (MQ=255)
                                                                                       cGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCtatag                                                         <  1:2065271/148‑5 (MQ=255)
                                                                                       cGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCtatag                                                         >  2:2266344/1‑144 (MQ=255)
                                                                                         tCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGAc                                                       >  2:573627/1‑148 (MQ=255)
                                                                                           ttGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTc                                                     >  1:2946454/1‑148 (MQ=255)
                                                                                               aGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCtatag                                                         >  2:3529601/1‑136 (MQ=255)
                                                                                               aGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGAt                                                 <  2:119227/148‑1 (MQ=255)
                                                                                                    ataCGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGaa                                            >  2:1851087/1‑148 (MQ=255)
                                                                                                     taCGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAac                                           >  1:4645622/1‑148 (MQ=255)
                                                                                                     taCGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAac                                           >  1:3539884/1‑148 (MQ=255)
                                                                                                      aCGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAaca                                          >  1:2659993/1‑148 (MQ=255)
                                                                                                       cGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAacac                                         >  2:4512999/1‑148 (MQ=255)
                                                                                                         gtgtTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAacacac                                       >  2:2504531/1‑148 (MQ=255)
                                                                                                           gtTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGa                                     <  1:715060/148‑1 (MQ=255)
                                                                                                           gtTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGa                                     <  1:2564322/148‑1 (MQ=255)
                                                                                                              gACTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAg                                  <  1:3384129/148‑1 (MQ=255)
                                                                                                               aCTGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGt                                 <  1:755102/148‑1 (MQ=255)
                                                                                                                 tGCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGtta                               >  2:3454451/1‑146 (MQ=255)
                                                                                                                  gCTAAACAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTTATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTAt                              >  2:2458750/1‑148 (MQ=255)
                                                                                                                     aaaCAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAACTCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAACACTCCAAGTAAGATTGAAAGCGATCTATAGACTCCGATTTGAACCCACGACAGTTATGct                           >  1:1637792/1‑148 (MQ=255)
                                                                                                                     aaaCAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGct                           >  2:3667068/1‑148 (MQ=255)
                                                                                                                     aaaCAGATTCAAGAATTACAAAAAAGCAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGct                           >  2:4605380/1‑148 (MQ=255)
                                                                                                                      aaCAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGctc                          >  2:951952/1‑148 (MQ=255)
                                                                                                                       aCAGATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGctct                         >  2:2621359/1‑148 (MQ=255)
                                                                                                                          gATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTc                      >  2:2026712/1‑148 (MQ=255)
                                                                                                                          gATTCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTc                      <  2:3068504/148‑1 (MQ=255)
                                                                                                                             tCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCgtg                   >  1:2981079/1‑148 (MQ=255)
                                                                                                                             tCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCgtg                   >  1:4113841/1‑148 (MQ=255)
                                                                                                                             tCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCgtg                   <  2:1050531/148‑1 (MQ=255)
                                                                                                                             tCAAGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCgtg                   >  1:2791621/1‑148 (MQ=255)
                                                                                                                                aggaTTTCAAAAAAGGGTTTCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATGGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGttt                <  2:3771150/145‑1 (MQ=255)
                                                                                                                                aGAATTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGttt                <  1:4512383/148‑1 (MQ=255)
                                                                                                                                  aaTTACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTa              <  2:1621859/148‑1 (MQ=255)
                                                                                                                                    ttACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACa            >  2:1963741/1‑148 (MQ=255)
                                                                                                                                    ttACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACa            >  2:842733/1‑148 (MQ=255)
                                                                                                                                     tACAAAAAAGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACAt           >  2:3162795/1‑148 (MQ=255)
                                                                                                                                             aGGAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCg   <  1:2237751/148‑1 (MQ=255)
                                                                                                                                              ggAGTGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGt  >  2:1740510/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACTGGTAAAACACAAACTGAACTCTGTAAAGATTATGGGGTATCTTCTTCTGCACTTGCAAAATGGATTAAGCAGTACTCTCAAGTGCGTCTTGAAGATAATACGGTGTTGACTGCTAAACAGATTCAAGAATTACAAAAAAGGAATGCCCAACTAGAGGAAGAAAATCTGATCTTAAAAAAAGCGAGTGCCATATTCATGCAAAACTCCAAGTAAGATTGAAAGCCATCT‑TAGACTCCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGT  >  CP043523/1882904‑1883192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑