BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,883,288	C→T	A3A (GCC→GCT)	FTN78_chr1928 →	Transposase TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,883,288	0	C	T	99.8%	1453.1 / NA	423	A3A (GCC→GCT)	FTN78_chr1928	Transposase TnpA
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (216/206); total (216/207)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 423 total aligned reads displayed.

GATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAA  >  CP043523/1883143‑1883431

gATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaa                                                                                                                                               <  2:1741268/148‑1 (MQ=255)

 aTTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaag                                                                                                                                              >  1:3306906/1‑148 (MQ=255)

 aTTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaag                                                                                                                                              >  2:143480/1‑148 (MQ=255)

  tttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaaga                                                                                                                                             >  2:3709702/1‑148 (MQ=255)

   ttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaa                                                                                                                                            >  1:2431214/1‑148 (MQ=255)

   ttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaa                                                                                                                                            >  1:2432006/1‑148 (MQ=255)

     gAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaa                                                                                                                                          >  2:3928585/1‑148 (MQ=255)

         acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                                                      <  1:2943519/148‑1 (MQ=255)

         acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                                                      <  2:4560695/148‑1 (MQ=255)

         acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                                                      >  1:645416/1‑148 (MQ=255)

            cGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTgg                                                                                                                                   >  2:213220/1‑148 (MQ=255)

              aCAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGaa                                                                                                                                 <  1:1710408/148‑1 (MQ=255)

               cAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAAc                                                                                                                                >  2:4332994/1‑148 (MQ=255)

                  ttaTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACgag                                                                                                                             <  2:2384031/146‑1 (MQ=255)

                       ctctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtt                                                                                                                        <  1:1851087/148‑1 (MQ=255)

                         ctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtttt                                                                                                                      <  1:179577/148‑1 (MQ=255)

                          tGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTa                                                                                                                     >  1:868200/1‑148 (MQ=255)

                            tCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaa                                                                                                                   >  1:2918918/1‑148 (MQ=255)

                            tCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaa                                                                                                                   <  1:1007244/148‑1 (MQ=255)

                            tCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaa                                                                                                                   <  2:48668/148‑1 (MQ=255)

                             cGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaaa                                                                                                                  >  2:1493144/1‑148 (MQ=255)

                               tgtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATc                                                                                                                >  2:2305520/1‑148 (MQ=255)

                                gtTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCa                                                                                                               >  1:2125476/1‑148 (MQ=255)

                                   ttACATGTCAATCGTTCCACCTACTGTAACTTCATGAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttc                                                                                                            <  1:1191986/148‑1 (MQ=255)

                                   ttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttc                                                                                                            >  1:1917174/1‑148 (MQ=255)

                                   ttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttc                                                                                                            <  2:614449/148‑1 (MQ=255)

                                    tACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCCTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttct                                                                                                           <  1:1654530/148‑1 (MQ=255)

                                     aCATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttctt                                                                                                          <  2:2955751/148‑1 (MQ=255)

                                      cATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttcttc                                                                                                         <  2:2981079/148‑1 (MQ=255)

                                         gTCAACCGTGCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttcttggag                                                                                                      <  2:4232968/148‑5 (MQ=255)

                                          tCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                                                     >  1:2266049/1‑148 (MQ=255)

                                          tCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                                                     <  1:1532149/148‑1 (MQ=255)

                                           cAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGt                                                                                                    >  1:50029/1‑148 (MQ=255)

                                           cAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGt                                                                                                    >  2:301592/1‑148 (MQ=255)

                                              tCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGAt                                                                                                 >  1:995632/1‑148 (MQ=255)

                                                gTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTa                                                                                               <  1:2089213/148‑1 (MQ=255)

                                                      cctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGt                                                                                         >  1:931894/8‑148 (MQ=255)

                                                      cctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGt                                                                                         >  1:781532/8‑148 (MQ=255)

                                                      cctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGt                                                                                         >  2:252970/8‑148 (MQ=255)

                                                       ctactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGtt                                                                                        >  1:830922/7‑148 (MQ=255)

                                                        tactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTa                                                                                       <  1:4223245/143‑1 (MQ=255)

                                                        tactgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTa                                                                                       >  1:3244256/6‑148 (MQ=255)

                                                         actgtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTaa                                                                                      >  2:673002/5‑148 (MQ=255)

                                                            gtaACTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAatat                                                                                   <  1:3162795/147‑1 (MQ=255)

                                                              aaCTTCATAAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATAttt                                                                                 >  2:3589472/1‑148 (MQ=255)

                                                                 ttcataAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTg                                                                              >  1:1159307/4‑148 (MQ=255)

                                                                  tcataAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGa                                                                             <  1:1077838/146‑1 (MQ=255)

                                                                  tcacaaaCAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGa                                                                             <  1:2023850/144‑1 (MQ=255)

                                                                    ataAACAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTATGAAg                                                                           >  2:3071398/1‑148 (MQ=255)

                                                                      aaaCAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGc                                                                         >  1:1942507/1‑148 (MQ=255)

                                                                        aCAAGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGccg                                                                       <  1:3512714/148‑3 (MQ=255)

                                                                          aaGAGGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTa                                                                     <  1:4599513/148‑1 (MQ=255)

                                                                           agagGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTAt                                                                    >  2:943929/1‑148 (MQ=255)

                                                                              ggCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCtt                                                                 <  2:2791621/148‑1 (MQ=255)

                                                                              ggCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCtt                                                                 <  1:4512999/148‑1 (MQ=255)

                                                                                ccTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTaa                                                               >  2:2674597/1‑148 (MQ=255)

                                                                                  ttCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGa                                                             >  2:2041905/1‑148 (MQ=255)

                                                                                   tCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGAc                                                            <  1:4647709/148‑1 (MQ=255)

                                                                                       aGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtct                                                        >  2:3432798/1‑148 (MQ=255)

                                                                                        gCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtctc                                                       >  2:363753/1‑148 (MQ=255)

                                                                                        gCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtctc                                                       <  1:2718621/148‑1 (MQ=255)

                                                                                        gCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtctc                                                       >  2:2514366/1‑148 (MQ=255)

                                                                                         cGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCa                                                      >  2:4055706/1‑148 (MQ=255)

                                                                                          gTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCaa                                                     <  2:1252892/148‑1 (MQ=255)

                                                                                             aaGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc                                                  >  1:2598451/1‑148 (MQ=255)

                                                                                             aaGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc                                                  >  1:3211777/1‑148 (MQ=255)

                                                                                             aaGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc                                                  >  1:4077167/1‑148 (MQ=255)

                                                                                                    aaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGaca                                           <  2:272421/148‑1 (MQ=255)

                                                                                                        cAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTcc                                       >  2:1639638/1‑148 (MQ=255)

                                                                                                         aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTccc                                      >  1:3065757/1‑148 (MQ=255)

                                                                                                         aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTccc                                      <  2:2744453/148‑1 (MQ=255)

                                                                                                         aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTccc                                      >  2:1802515/1‑148 (MQ=255)

                                                                                                                   aaaattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCa                            >  2:3215295/8‑148 (MQ=255)

                                                                                                                    aaattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCaa                           >  1:3347733/7‑148 (MQ=255)

                                                                                                                     aattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAAc                          <  1:3660749/143‑1 (MQ=255)

                                                                                                                     aattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAAc                          >  2:4124116/6‑148 (MQ=255)

                                                                                                                     aattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAAc                          >  1:249662/6‑148 (MQ=255)

                                                                                                                        tactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAt                       >  1:2474985/3‑148 (MQ=255)

                                                                                                                          ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtca                     >  2:1144555/1‑146 (MQ=255)

                                                                                                                          ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtca                     <  2:1594812/148‑3 (MQ=255)

                                                                                                                          ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtca                     <  2:3869656/148‑3 (MQ=255)

                                                                                                                           tcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtcaa                    >  2:812891/1‑145 (MQ=255)

                                                                                                                           tcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtcaa                    >  2:2886137/1‑145 (MQ=255)

                                                                                                                            cctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATcaaa                   >  1:1465774/1‑148 (MQ=255)

                                                                                                                             ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAAc                  <  2:4486214/148‑1 (MQ=255)

                                                                                                                               tGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCt                >  2:399596/1‑148 (MQ=255)

                                                                                                                                gagaTTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCtt               <  1:713385/148‑2 (MQ=255)

                                                                                                                                 agaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCttg              >  1:754220/1‑146 (MQ=255)

                                                                                                                                  gaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGt             <  1:2160341/148‑1 (MQ=255)

                                                                                                                                   aTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtt            >  1:1384761/1‑148 (MQ=255)

                                                                                                                                     ttATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtttt          >  2:2930515/1‑148 (MQ=255)

                                                                                                                                         atGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAt      <  2:2160281/148‑1 (MQ=255)

                                                                                                                                         atGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAt      <  1:4345556/148‑1 (MQ=255)

                                                                                                                                          tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc     <  2:1922736/148‑1 (MQ=255)

                                                                                                                                          tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc     >  1:3782310/1‑148 (MQ=255)

                                                                                                                                           gAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa    <  1:2375943/148‑1 (MQ=255)

                                                                                                                                           gAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa    <  2:1658983/148‑1 (MQ=255)

                                                                                                                                           gAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa    >  2:2214549/1‑148 (MQ=255)

                                                                                                                                            aaaGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaa   <  1:3928585/148‑1 (MQ=255)

                                                                                                                                             aaGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaa  >  2:3244754/1‑148 (MQ=255)

GATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAA  >  CP043523/1883143‑1883431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`