BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,883,361	T→C	C28R (TGT→CGT)	FTN78_chr1928 →	Transposase TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,883,361	0	T	C	99.8%	1659.6 / ‑5.1	454	C28R (TGT→CGT)	FTN78_chr1928	Transposase TnpA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (254/199); minor base A (0/1); total (254/200)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 454 total aligned reads displayed.

CAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGG  >  CP043523/1883216‑1883504

    agGCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCt                                                                                                                                           >  1:4110775/1‑148 (MQ=255)

     ggCCTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCtt                                                                                                                                          <  1:4512999/148‑1 (MQ=255)

        cTTCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAg                                                                                                                                       <  2:4125415/148‑1 (MQ=255)

          tCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGAc                                                                                                                                     <  2:2125476/148‑1 (MQ=255)

          tCGAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGAc                                                                                                                                     <  1:4647709/148‑1 (MQ=255)

            gAAGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACtt                                                                                                                                   >  2:3033067/1‑148 (MQ=255)

             aaGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtc                                                                                                                                  <  1:2355181/148‑1 (MQ=255)

              aGCGTGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtct                                                                                                                                 >  2:3432798/1‑148 (MQ=255)

                  tGAAGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAg                                                                                                                             >  2:1803914/1‑148 (MQ=255)

                    aaGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc                                                                                                                           <  2:3002528/148‑1 (MQ=255)

                    aaGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc                                                                                                                           >  1:3211777/1‑148 (MQ=255)

                     aGTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCt                                                                                                                          <  2:2740894/148‑1 (MQ=255)

                         gaaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGa                                                                                                                      >  1:1764908/2‑148 (MQ=255)

                          aaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGac                                                                                                                     >  2:3256550/1‑148 (MQ=255)

                          aaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGac                                                                                                                     <  1:2124770/148‑1 (MQ=255)

                            aaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTTAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGacac                                                                                                                   >  2:938398/1‑148 (MQ=255)

                             aTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACt                                                                                                                  >  2:4364522/1‑148 (MQ=255)

                                aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTccc                                                                                                               >  1:3065757/1‑148 (MQ=255)

                                           aaattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCaa                                                                                                    <  1:2030293/142‑1 (MQ=255)

                                            aattactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAAc                                                                                                   >  1:249662/6‑148 (MQ=255)

                                             attactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAAcc                                                                                                  <  2:758849/144‑1 (MQ=255)

                                             attactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAAcc                                                                                                  >  2:222539/5‑148 (MQ=255)

                                               tactcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAt                                                                                                >  1:2474985/3‑148 (MQ=255)

                                                 ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAACATGTCAACCAtca                                                                                              >  2:2913434/1‑146 (MQ=255)

                                                 ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtca                                                                                              <  2:3869656/148‑3 (MQ=255)

                                                 ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtca                                                                                              <  2:1594812/148‑3 (MQ=255)

                                                 ctcctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCAtca                                                                                              >  1:508119/1‑146 (MQ=255)

                                                    ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAAc                                                                                           <  2:3972384/148‑1 (MQ=255)

                                                    ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAAc                                                                                           <  2:4486214/148‑1 (MQ=255)

                                                       gagaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCtt                                                                                        >  2:1714936/1‑147 (MQ=255)

                                                        agaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCttg                                                                                       >  1:754228/1‑146 (MQ=255)

                                                         gaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGt                                                                                      <  1:2160294/148‑1 (MQ=255)

                                                          aTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtt                                                                                     >  1:1384761/1‑148 (MQ=255)

                                                            ttATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtttt                                                                                   >  2:4546629/1‑148 (MQ=255)

                                                              atatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTaa                                                                                 >  1:1678189/1‑148 (MQ=255)

                                                                 tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                              >  2:275384/1‑148 (MQ=255)

                                                                 tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                              >  1:900380/1‑148 (MQ=255)

                                                                 tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                              >  2:2393924/1‑148 (MQ=255)

                                                                 tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                              <  1:457948/148‑1 (MQ=255)

                                                                 tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                              <  2:1922736/148‑1 (MQ=255)

                                                                   aaaGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaa                                                                            <  1:2924628/148‑1 (MQ=255)

                                                                    aaGCTAAGAAAAGAATTGGGACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaa                                                                           >  2:2520962/1‑148 (MQ=255)

                                                                     agctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaat                                                                          >  1:4707100/5‑148 (MQ=255)

                                                                     agctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaat                                                                          <  1:623093/144‑1 (MQ=255)

                                                                       ctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaataa                                                                        <  2:2592218/146‑1 (MQ=255)

                                                                          agaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAGTGTCAACCATCAAACCTTGTTTTAAATCAAATAAAtc                                                                     <  2:3646141/148‑1 (MQ=255)

                                                                          agaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAAtc                                                                     >  1:3365944/1‑148 (MQ=255)

                                                                           gaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAAtct                                                                    <  2:4088470/148‑1 (MQ=255)

                                                                              aagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCt                                                                 <  1:4042082/148‑1 (MQ=255)

                                                                                 aaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTa                                                              >  1:3849637/1‑148 (MQ=255)

                                                                                   ttGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAtt                                                            >  2:622278/1‑148 (MQ=255)

                                                                                   ttGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAtt                                                            >  1:4423764/1‑148 (MQ=255)

                                                                                   ttGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGCTTTAAATCAAATAAATCTCCTGTAtt                                                            <  2:4623875/148‑1 (MQ=255)

                                                                                    tGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAttt                                                           >  2:3510199/1‑148 (MQ=255)

                                                                                    tGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAttt                                                           >  1:714830/1‑148 (MQ=255)

                                                                                    tGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAttt                                                           >  1:1468995/1‑148 (MQ=255)

                                                                                       aaCGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTttct                                                        >  1:2638521/1‑148 (MQ=255)

                                                                                        aCGAGAGCTTTTAAAATTATTTTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTttctt                                                       >  2:1288568/1‑148 (MQ=255)

                                                                                         cGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTttcttc                                                      <  1:3278003/148‑1 (MQ=255)

                                                                                         cGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTttcttc                                                      >  1:2693221/1‑148 (MQ=255)

                                                                                          gagaGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTtcttct                                                     >  2:3177182/1‑148 (MQ=255)

                                                                                           agagCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTg                                                    >  1:862251/1‑148 (MQ=255)

                                                                                            gagCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGa                                                   >  1:112321/1‑148 (MQ=255)

                                                                                            gagCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGa                                                   >  1:4374932/1‑148 (MQ=255)

                                                                                             agCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGAt                                                  <  2:1051232/148‑1 (MQ=255)

                                                                                              gCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATa                                                 >  1:155756/1‑148 (MQ=255)

                                                                                                ttttAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCAGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAAt                                               <  1:2894819/148‑1 (MQ=255)

                                                                                                 tttAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATc                                              >  1:1035439/1‑148 (MQ=255)

                                                                                                  ttAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCt                                             >  2:2040078/1‑148 (MQ=255)

                                                                                                     aaaTCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCt                                          >  1:3711625/1‑148 (MQ=255)

                                                                                                     aaaTCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCt                                          <  2:3307338/148‑1 (MQ=255)

                                                                                                      aaTCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCtt                                         >  2:4256722/1‑148 (MQ=255)

                                                                                                      aaTCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCt                                          <  2:392866/147‑1 (MQ=255)

                                                                                                      aaTCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCt                                          >  1:392866/1‑147 (MQ=255)

                                                                                                        tCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTaa                                       <  2:3224965/148‑1 (MQ=255)

                                                                                                          aTTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAAc                                     <  1:3523295/148‑1 (MQ=255)

                                                                                                             cttctGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAg                                  >  1:1423173/1‑148 (MQ=255)

                                                                                                             cttctGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAg                                  >  1:2384033/1‑148 (MQ=255)

                                                                                                                 tGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAAtt                              <  1:4210533/148‑1 (MQ=255)

                                                                                                                   cGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTa                            >  1:4324811/1‑148 (MQ=255)

                                                                                                                   cGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTa                            >  1:1935886/1‑148 (MQ=255)

                                                                                                                    gTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTaa                           <  2:1501809/148‑1 (MQ=255)

                                                                                                                      gATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAAcc                         >  2:2164306/1‑148 (MQ=255)

                                                                                                                      gATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAAcc                         >  2:2701552/1‑148 (MQ=255)

                                                                                                                       aTTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAAccc                        >  2:3479882/1‑148 (MQ=255)

                                                                                                                       aTTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCAAAAATGCCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAAccc                        >  1:797177/1‑148 (MQ=255)

                                                                                                                         tATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGa                      <  2:473431/148‑1 (MQ=255)

                                                                                                                           tGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAt                    >  2:3800341/1‑148 (MQ=255)

                                                                                                                           tGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAt                    >  1:2136225/1‑148 (MQ=255)

                                                                                                                            ggCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAtt                   >  2:1407542/1‑148 (MQ=255)

                                                                                                                             gCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCGAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCACATAAATCTCCTGTATTTTCTTCGGATAATCTGCTTAAACAAGAATTTAACCCGAATTc                  <  2:1672403/148‑1 (MQ=255)

                                                                                                                             gCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTc                  >  1:1189532/1‑148 (MQ=255)

                                                                                                                              cGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTcc                 <  1:1833501/148‑1 (MQ=255)

                                                                                                                                 tAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCa              >  2:845238/1‑148 (MQ=255)

                                                                                                                                   atatTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCaaa            <  2:1154991/148‑1 (MQ=255)

                                                                                                                                     atTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATc          >  1:1881743/1‑148 (MQ=255)

                                                                                                                                      tttCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCa         >  2:2793000/1‑148 (MQ=255)

                                                                                                                                        tCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAg       <  1:3630124/148‑1 (MQ=255)

                                                                                                                                         cTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGt      <  1:2971817/148‑1 (MQ=255)

                                                                                                                                          tGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGtt     <  1:1236518/148‑1 (MQ=255)

CAAGAGGCCTTCGAAGCGTGAAGTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGG  >  CP043523/1883216‑1883504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`