Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,883,411 A→C I44I (ATA→ATC)  FTN78_chr1928 → Transposase TnpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,883,4110AC99.8% 1768.9 / ‑5.2 482I44I (ATA→ATC) FTN78_chr1928Transposase TnpA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (248/233);  total (249/233)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 483 total aligned reads displayed.

CCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCT  >  CP043523/1883267‑1883554
                                                                                                                                                |                                                                                                                                               
 ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAAc                                                                                                                                             <  2:1526220/148‑1 (MQ=255)
 ctTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAAc                                                                                                                                             <  2:4486214/148‑1 (MQ=255)
    gagaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCtt                                                                                                                                          >  2:1714936/1‑147 (MQ=255)
    gagaTTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCtt                                                                                                                                          <  1:713385/148‑2 (MQ=255)
     agaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCttg                                                                                                                                         >  1:754228/1‑146 (MQ=255)
      gaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGt                                                                                                                                        <  1:430176/148‑1 (MQ=255)
      gaTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGt                                                                                                                                        <  1:2160294/148‑1 (MQ=255)
       aTTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtt                                                                                                                                       <  1:4410684/148‑1 (MQ=255)
         ttATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGtttt                                                                                                                                     <  2:3703391/148‑1 (MQ=255)
          tataTGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTa                                                                                                                                    <  1:2753787/148‑1 (MQ=255)
          tataTGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTa                                                                                                                                    >  2:1817367/1‑148 (MQ=255)
           atatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTaa                                                                                                                                   >  1:3271392/1‑148 (MQ=255)
           atatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTaa                                                                                                                                   <  1:782453/148‑1 (MQ=255)
            tatGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTaaa                                                                                                                                  >  2:237137/1‑148 (MQ=255)
             atGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAAt                                                                                                                                 <  2:2160281/148‑1 (MQ=255)
              tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                                                                                >  2:275384/1‑148 (MQ=255)
              tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                                                                                <  1:457948/148‑1 (MQ=255)
              tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                                                                                >  1:900380/1‑148 (MQ=255)
              tGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATc                                                                                                                                <  2:1922736/148‑1 (MQ=255)
               gAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa                                                                                                                               >  2:300573/1‑148 (MQ=255)
               gAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCa                                                                                                                               >  2:2214549/1‑148 (MQ=255)
                 aaGCTAAGAAAAGAATTGGGACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaa                                                                                                                             >  2:2520962/1‑148 (MQ=255)
                  agctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaat                                                                                                                            <  1:623093/144‑1 (MQ=255)
                  agctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaat                                                                                                                            >  1:4707100/5‑148 (MQ=255)
                   gctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaata                                                                                                                           <  1:854797/145‑1 (MQ=255)
                    ctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaataa                                                                                                                          <  1:3358628/146‑1 (MQ=255)
                    ctaagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaataa                                                                                                                          <  2:2592218/146‑1 (MQ=255)
                     taagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaataaa                                                                                                                         <  2:664254/147‑1 (MQ=255)
                      aagaaaagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCaaataaat                                                                                                                        >  2:4537274/1‑148 (MQ=255)
                           aagaaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCt                                                                                                                   <  1:147961/148‑1 (MQ=255)
                              aaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTa                                                                                                                <  2:868200/148‑1 (MQ=255)
                              aaTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTa                                                                                                                <  2:931093/148‑1 (MQ=255)
                               aTTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAt                                                                                                               <  2:1159307/148‑1 (MQ=255)
                                ttGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGCTTTAAATCAAATAAATCTCCTGTAtt                                                                                                              <  2:4623875/148‑1 (MQ=255)
                                 tGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAttt                                                                                                             >  2:3510199/1‑148 (MQ=255)
                                 tGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTAttt                                                                                                             >  1:4454311/1‑148 (MQ=255)
                                     aCGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTttctt                                                                                                         >  2:3065384/1‑148 (MQ=255)
                                      cGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTCTTTTAAATCAAATAAATGTCCTGTATTctcttc                                                                                                        >  1:1268814/1‑148 (MQ=255)
                                       gagaGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTtcttct                                                                                                       >  2:3177182/1‑148 (MQ=255)
                                         gagCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGa                                                                                                     >  1:2924376/1‑148 (MQ=255)
                                           gCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATa                                                                                                   <  1:4067370/148‑1 (MQ=255)
                                               ttAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCt                                                                                               >  2:2040078/1‑148 (MQ=255)
                                                  aaaTCATTCTTCCGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCt                                                                                            >  1:3596347/1‑148 (MQ=255)
                                                     tCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTaa                                                                                         <  2:3224003/148‑1 (MQ=255)
                                                         tcttctGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACaa                                                                                     >  2:4165579/1‑148 (MQ=255)
                                                          cttctGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAg                                                                                    >  2:2912224/1‑148 (MQ=255)
                                                          cgtctGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAg                                                                                    <  2:3333070/146‑1 (MQ=255)
                                                              tGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAAtt                                                                                <  1:169212/148‑1 (MQ=255)
                                                                    aTTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAAccc                                                                          >  2:3479882/1‑148 (MQ=255)
                                                                       aTGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGaa                                                                       <  1:2686395/148‑1 (MQ=255)
                                                                        tGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAt                                                                      <  1:143480/148‑1 (MQ=255)
                                                                        tGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAt                                                                      >  2:3800341/1‑148 (MQ=255)
                                                                        tGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAt                                                                      >  1:2135735/1‑148 (MQ=255)
                                                                         ggCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAAtt                                                                     >  1:156779/1‑148 (MQ=255)
                                                                          gCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTc                                                                    >  1:1189532/1‑148 (MQ=255)
                                                                          gCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTc                                                                    >  2:340174/1‑148 (MQ=255)
                                                                               aaTATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCaa                                                               <  1:4332994/148‑1 (MQ=255)
                                                                               aaTATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCaa                                                               >  1:503639/1‑148 (MQ=255)
                                                                                atatTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCaaa                                                              <  1:252970/148‑1 (MQ=255)
                                                                                atatTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCaaa                                                              >  2:4029330/1‑148 (MQ=255)
                                                                                 tatTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAAt                                                             <  2:1328115/148‑1 (MQ=255)
                                                                                  atTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAATAATTTAACCCGAATTCCCCAAATc                                                            >  1:3718185/1‑148 (MQ=255)
                                                                                  atTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATc                                                            >  1:1881743/1‑148 (MQ=255)
                                                                                        gAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGttt                                                      >  1:3522582/1‑148 (MQ=255)
                                                                                         aaGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTg                                                     <  1:1382283/148‑1 (MQ=255)
                                                                                             ccgTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGaca                                                 >  1:369368/3‑148 (MQ=255)
                                                                                              cgTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGacaa                                                >  2:492641/2‑148 (MQ=255)
                                                                                               gTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGacaac                                               <  2:3875218/148‑1 (MQ=255)
                                                                                                 aTCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTCTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAg                                             >  2:4674245/1‑148 (MQ=255)
                                                                                                  tCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGa                                            >  2:2256166/1‑148 (MQ=255)
                                                                                                  tCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGa                                            >  2:4365545/1‑148 (MQ=255)
                                                                                                        gACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCAc                                      >  2:3276405/1‑148 (MQ=255)
                                                                                                          cTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACtt                                    <  1:3966599/148‑1 (MQ=255)
                                                                                                          cTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACtt                                    >  1:1222545/1‑148 (MQ=255)
                                                                                                          cTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACtt                                    >  2:1060265/1‑148 (MQ=255)
                                                                                                           ttCTCAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTta                                   <  2:1411022/148‑1 (MQ=255)
                                                                                                            tctcAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTtat                                  <  2:2431215/148‑1 (MQ=255)
                                                                                                            tctcAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTtat                                  <  1:1144555/148‑1 (MQ=255)
                                                                                                             ctcAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTtata                                 <  1:2962499/148‑1 (MQ=255)
                                                                                                               cAAGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATAtc                               >  1:1024921/1‑148 (MQ=255)
                                                                                                                aaGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATAtct                              <  1:1639820/148‑1 (MQ=255)
                                                                                                                 aGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATAtctc                             >  2:2451082/1‑148 (MQ=255)
                                                                                                                 aGTCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATAtctc                             <  1:2393924/148‑1 (MQ=255)
                                                                                                                   tCTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCta                           >  2:3956674/1‑148 (MQ=255)
                                                                                                                    cTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCtat                          <  1:3010189/148‑1 (MQ=255)
                                                                                                                    cTATGACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCtat                          <  2:4110775/148‑1 (MQ=255)
                                                                                                                        gACACTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGa                      >  1:232772/1‑148 (MQ=255)
                                                                                                                         acacTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAg                     >  1:3078806/1‑148 (MQ=255)
                                                                                                                         acacTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAg                     <  1:363753/148‑1 (MQ=255)
                                                                                                                           acTCCCTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCt                   >  1:1067987/1‑148 (MQ=255)
                                                                                                                              cccTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAg                >  1:4535224/1‑148 (MQ=255)
                                                                                                                               ccTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGc               <  1:2908914/148‑1 (MQ=255)
                                                                                                                                cTAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCg              <  1:3921573/148‑1 (MQ=255)
                                                                                                                                 tAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGa             <  1:3469248/148‑1 (MQ=255)
                                                                                                                                 tAAAATGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGa             >  1:4179147/1‑148 (MQ=255)
                                                                                                                                  aaaaTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGAc            >  1:2072839/1‑148 (MQ=255)
                                                                                                                                   aaaTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACa           <  1:1817367/148‑1 (MQ=255)
                                                                                                                                     aTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATg         <  1:3215295/148‑1 (MQ=255)
                                                                                                                                     aTGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATg         <  1:1933825/148‑1 (MQ=255)
                                                                                                                                      tGTCAACCATCAAACCTTGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGt        <  2:755067/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCTAAAATGTCAACCATAAAACCTCGTTTTAAATCAAATAAATCTCCTGTATTTTCTTCTGATAATCTGCTTAAACAAGAATTTAACCCGAATTCCCCAAATCAAGTTTGGACAACAGATTTCACTTATATCTCTATAGGAGCTAAGCGACATGTTTATCT  >  CP043523/1883267‑1883554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑