BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,883,804	T→G	D18E (GAT→GAG)	FTN78_chr1929 →	Transposase TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,883,804	0	T	G	99.6%	2970.1 / ‑6.3	820	D18E (GAT→GAG)	FTN78_chr1929	Transposase TnpA
Reads supporting (aligned to +/- strand): ref base T (0/3); new base G (418/398); total (418/402)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 820 total aligned reads displayed.

GGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCCTATGATAATGCCGTCACTGATGTCTTTTTCAAGTATCTTAAACAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAATGGTTTAACTCCA  >  CP043523/1883693‑1883948

                              ttttAGAAAGTTATTAGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGtt                                                                                >  1:1431169/2‑148 (MQ=255)

                               tttAGAAAGTTATTAGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTc                                                                               <  2:4436562/148‑1 (MQ=255)

                               tttAGAAAGTTATTAGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTc                                                                               >  1:1830764/1‑148 (MQ=255)

                                 tAGAAAGTTATTAGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCaa                                                                             <  2:3576036/148‑1 (MQ=255)

                                           ttaGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCt                                                                   >  2:3622632/1‑148 (MQ=255)

                                            taGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATATTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCtt                                                                  >  2:748413/1‑148 (MQ=255)

                                             aGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCttt                                                                 <  2:836233/148‑1 (MQ=255)

                                             aGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCttt                                                                 >  2:1338243/1‑148 (MQ=255)

                                                  tatttgaaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATa                                                            <  2:1546710/142‑1 (MQ=255)

                                                  tatttgaaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATa                                                            >  2:382919/7‑148 (MQ=255)

                                                  aGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATa                                                            <  1:3455452/148‑1 (MQ=255)

                                                    cATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACa                                                          <  1:1575170/148‑1 (MQ=255)

                                                      tgaaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATc                                                        <  2:3035560/146‑1 (MQ=255)

                                                       gaaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCg                                                       >  1:625789/2‑148 (MQ=255)

                                                       gaaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCg                                                       <  1:2224762/147‑1 (MQ=255)

                                                       gaaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCg                                                       <  1:305032/147‑1 (MQ=255)

                                                        aaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGa                                                      <  1:724022/148‑1 (MQ=255)

                                                        aaTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGa                                                      >  2:4312722/1‑148 (MQ=255)

                                                         aTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGaa                                                     <  1:10194/148‑1 (MQ=255)

                                                         aTTGCTTGCTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGaa                                                     <  1:3881325/148‑1 (MQ=255)

                                                          ttgcttgcTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAAc                                                    >  2:3421443/1‑148 (MQ=255)

                                                          ttgcttgcTTCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTCTCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAAc                                                    >  2:4625513/1‑148 (MQ=255)

                                                            gcttgcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACaa                                                  <  2:4139136/148‑1 (MQ=255)

                                                              ttgcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAtt                                                <  1:2048703/148‑1 (MQ=255)

                                                              ttgcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAtt                                                <  1:2705760/148‑1 (MQ=255)

                                                              ttgcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAtt                                                <  1:511208/148‑1 (MQ=255)

                                                              cgatttcttACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAtt                                                >  2:351061/5‑148 (MQ=255)

                                                               tgcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAttt                                               >  1:4464476/1‑148 (MQ=255)

                                                               tgcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAttt                                               >  1:1157044/1‑148 (MQ=255)

                                                               tgcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAttt                                               >  1:3366806/1‑148 (MQ=255)

                                                                gcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAtttt                                              >  1:3790087/1‑148 (MQ=255)

                                                                gcttCTTACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAAtttt                                              <  2:2453488/148‑1 (MQ=255)

                                                                  ttcttACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTtat                                            >  2:3144475/1‑148 (MQ=255)

                                                                  ttcttACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTtat                                            >  1:2357478/1‑148 (MQ=255)

                                                                    cttACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATaa                                          >  1:4162149/1‑148 (MQ=255)

                                                                     ttACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAAc                                         >  2:2843424/1‑148 (MQ=255)

                                                                     ttACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTCGCTTTGAATACATCGAACAATTTTATAAc                                         >  1:3982764/1‑148 (MQ=255)

                                                                     gtccTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAAc                                         <  2:3250624/145‑1 (MQ=255)

                                                                      tACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACa                                        <  1:4334695/148‑1 (MQ=255)

                                                                      tACTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACa                                        <  1:4658992/148‑1 (MQ=255)

                                                                       aCTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACaa                                       <  2:930890/148‑1 (MQ=255)

                                                                       aCTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACaa                                       >  1:1720300/1‑148 (MQ=255)

                                                                        cTCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAAt                                      >  1:2904903/1‑148 (MQ=255)

                                                                         tCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAAtt                                     >  2:3211403/1‑148 (MQ=255)

                                                                         tCCAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAAtt                                     <  1:405163/148‑1 (MQ=255)

                                                                           cAAGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATtat                                   >  2:219808/1‑148 (MQ=255)

                                                                            aaGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATtata                                  >  2:399461/1‑148 (MQ=255)

                                                                            aaGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATtata                                  <  1:2606376/148‑1 (MQ=255)

                                                                             aGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATaa                                 <  1:924172/148‑1 (MQ=255)

                                                                             aGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATaa                                 <  2:1913948/148‑1 (MQ=255)

                                                                             aGCCTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATaa                                 <  2:4569265/148‑1 (MQ=255)

                                                                              gcatGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAAt                                <  1:1069983/145‑1 (MQ=255)

                                                                               ccTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATc                               >  1:1624026/1‑148 (MQ=255)

                                                                               ccTGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATc                               <  1:239339/148‑1 (MQ=255)

                                                                                 tGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCt                             >  1:1966587/1‑148 (MQ=255)

                                                                                 tGGATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCt                             >  2:2055022/1‑148 (MQ=255)

                                                                                  ggATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTc                            >  1:1319973/1‑148 (MQ=255)

                                                                                  ggATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTc                            >  1:2943628/1‑148 (MQ=255)

                                                                                   gATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCa                           <  2:2736831/148‑1 (MQ=255)

                                                                                   gATATCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCa                           <  1:2528763/148‑1 (MQ=255)

                                                                                    atatCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCAt                          >  2:1069740/1‑148 (MQ=255)

                                                                                    atatCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCAt                          >  2:3743263/1‑148 (MQ=255)

                                                                                    atatCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCAt                          >  1:2450610/1‑148 (MQ=255)

                                                                                     tatCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCAtt                         >  1:2025283/1‑148 (MQ=255)

                                                                                     tatCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCAtt                         >  1:3527405/1‑148 (MQ=255)

                                                                                      atCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTc                        <  2:1649698/148‑1 (MQ=255)

                                                                                      atCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTc                        <  2:2541046/148‑1 (MQ=255)

                                                                                      atCCTTATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTc                        <  2:525750/148‑1 (MQ=255)

                                                                                        ccttATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCg                      >  1:2187910/4‑148 (MQ=255)

                                                                                        ccttATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCg                      >  2:2369902/4‑148 (MQ=255)

                                                                                          ttATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGcc                    >  2:4274218/2‑148 (MQ=255)

                                                                                          ttATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGcc                    <  1:3233750/147‑1 (MQ=255)

                                                                                           tATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCca                   >  2:3345042/1‑148 (MQ=255)

                                                                                           tATGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCca                   <  2:116931/148‑1 (MQ=255)

                                                                                            aTGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaa                  <  1:3627329/148‑1 (MQ=255)

                                                                                            aTGATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaa                  <  2:3465723/148‑1 (MQ=255)

                                                                                            aTGATAATGCCGTCACTGAGGTCTTTTTCAAGAATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaa                  <  1:4359174/148‑1 (MQ=255)

                                                                                              gATAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaaca                >  1:2816854/1‑148 (MQ=255)

                                                                                               aTAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaa               <  2:1829353/148‑1 (MQ=255)

                                                                                                tAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaag              >  2:1744174/1‑147 (MQ=255)

                                                                                                tAATGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaag              <  1:3402170/148‑2 (MQ=255)

                                                                                                 aaTGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaagg             <  2:2663405/148‑3 (MQ=255)

                                                                                                 aaTGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaagg             <  2:652622/148‑3 (MQ=255)

                                                                                                 aaTGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaagg             >  2:2304139/1‑146 (MQ=255)

                                                                                                 aaTGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaagg             >  2:2788410/1‑146 (MQ=255)

                                                                                                 aaTGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaagg             <  2:2931182/148‑3 (MQ=255)

                                                                                                  aTGCCGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCcaacaaggg            >  2:1312311/1‑145 (MQ=255)

                                                                                                     ccGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGttt         <  1:3684280/148‑1 (MQ=255)

                                                                                                     ccGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGttt         >  2:1447317/1‑148 (MQ=255)

                                                                                                      cGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTa        <  1:3595794/148‑1 (MQ=255)

                                                                                                      cGTCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTa        >  1:1262447/1‑148 (MQ=255)

                                                                                                        tCACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTAAc      <  1:2748491/148‑1 (MQ=255)

                                                                                                         cACTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAATGGTTTAACt     <  2:3895329/148‑1 (MQ=255)

                                                                                                         cACTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTAACt     >  1:4602438/1‑148 (MQ=255)

                                                                                                          aCTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTAACTc    <  1:3104340/148‑1 (MQ=255)

                                                                                                          aCTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTAACTc    >  1:98184/1‑148 (MQ=255)

                                                                                                           cTGAGGTCTTTTTCAAGTATCTTAAACATCGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAATGGTTTAACTcc   >  1:2111261/1‑148 (MQ=255)

                                                                                                           cTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTAACTcc   >  1:810901/1‑148 (MQ=255)

                                                                                                           cTGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTAACTcc   <  1:1473702/148‑1 (MQ=255)

                                                                                                            tGAGGTCTTTTTCAAGTATCTTAAAAAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAAGGGTTTAACTCCa  <  2:4070540/148‑1 (MQ=255)

GGATCAAGGAAGCCAATTTAAATCAGCTTCCTTTAGAAAGTTATTAGATGAGCATCAATTGCTTGCTTCTTACTCCAAGCCTGGATATCCCTATGATAATGCCGTCACTGATGTCTTTTTCAAGTATCTTAAACAACGAGAAATTAATCGCAGAACTTATCACTCCATTCAAGAAGTTCAACTCTCTTGCTTTGAATACATCGAACAATTTTATAACAATTATAATCCTCATTCCGCCAACAATGGTTTAACTCCA  >  CP043523/1883693‑1883948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`