Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,848,324 3 bp→TAC coding (33‑35/201 nt) FTN78_chr1898 → Cold shock protein of CSP family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,848,3240AT99.1% 190.8 / ‑4.0 115A11A (GCA→GCT) FTN78_chr1898Cold shock protein of CSP family
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (84/30);  total (84/31)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
*CP0435231,848,3250GA99.1% 189.1 / ‑4.3 115D12T (GAT→ACT) FTN78_chr1898Cold shock protein of CSP family
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (84/30);  total (84/31)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01
*CP0435231,848,3260AC98.3% 187.5 / ‑4.1 115D12T (GAT→ACT) FTN78_chr1898Cold shock protein of CSP family
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (83/30);  total (84/31)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑ATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCT  >  CP043523/1848187‑1848382
                                                                                                                                              |||                                                        
aTTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAgg                                                    >  2:2824422/1‑148 (MQ=11)
   aGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                 >  2:2792447/1‑148 (MQ=11)
   aGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                 >  2:2791848/1‑148 (MQ=11)
   aGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAtt                                                 >  1:2439640/1‑148 (MQ=11)
   aGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTAATAAAGGAtt                                                 >  2:2458300/1‑148 (MQ=14)
    gtgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAttt                                                >  1:398047/1‑148 (MQ=14)
    gtgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGAttt                                                >  1:3178557/1‑148 (MQ=11)
     tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               <  2:35883/148‑1 (MQ=11)
     tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               <  1:846853/148‑1 (MQ=11)
     tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               >  1:512446/1‑148 (MQ=11)
     tgtTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTg                                               >  1:1725130/1‑148 (MQ=11)
       ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                             <  2:2115304/148‑2 (MQ=11)
       ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                             >  2:3207640/1‑147 (MQ=11)
       ttattaTAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggt                                             >  1:2822905/1‑147 (MQ=11)
        tattatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggtt                                            <  2:2749257/148‑3 (MQ=11)
         attatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTggttt                                           >  1:2469618/1‑145 (MQ=11)
         attatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                           >  1:533871/1‑148 (MQ=11)
         attatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGttt                                           <  2:4431737/148‑1 (MQ=11)
          ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          <  1:4257867/148‑1 (MQ=11)
          ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          >  2:3992256/1‑148 (MQ=11)
          ttatAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          >  2:20720/1‑148 (MQ=11)
          ttatAAAGAAGTAGATTTTGACATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAACTGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGtttt                                          <  2:1405273/148‑1 (MQ=11)
            ataaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAt                                        <  2:3692140/145‑1 (MQ=11)
            ataaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAt                                        <  2:3222903/145‑1 (MQ=11)
            ataaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAt                                        >  2:2624075/4‑148 (MQ=11)
              aaagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAtta                                      >  1:1963178/2‑146 (MQ=11)
               aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttac                                     >  1:2420193/1‑145 (MQ=11)
               aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttac                                     >  2:453377/1‑145 (MQ=11)
               aagaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAc                                     >  2:747105/1‑148 (MQ=11)
                agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacc                                    >  2:1780777/1‑144 (MQ=11)
                agaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacc                                    >  2:70120/1‑144 (MQ=11)
                 gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  2:3629304/1‑143 (MQ=11)
                 gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  2:3628939/1‑143 (MQ=11)
                 gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  2:1458896/1‑143 (MQ=11)
                 gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  1:582318/1‑143 (MQ=11)
                 gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTAttacct                                   >  1:3769553/1‑143 (MQ=11)
                 gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAcct                                   >  2:1458577/1‑146 (MQ=11)
                 gaagTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTAcct                                   >  1:3769515/1‑146 (MQ=11)
                     tagaTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtga                               <  2:1190884/145‑1 (MQ=11)
                        aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                            >  2:741771/1‑148 (MQ=11)
                        aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                            >  1:91649/1‑148 (MQ=11)
                        aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                            <  2:44544/148‑1 (MQ=11)
                        aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaaga                            <  2:252845/148‑1 (MQ=11)
                        aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGCTTTATCACTTCagaaga                            >  1:1115481/1‑148 (MQ=11)
                        aTTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑ATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGaagaag                          <  2:1321597/148‑1 (MQ=17)
                         ttttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagat                           <  2:4278147/148‑2 (MQ=11)
                         ttttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagat                           <  1:1314774/148‑2 (MQ=11)
                          tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  2:4678738/1‑146 (MQ=11)
                          tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  1:2096044/1‑146 (MQ=11)
                          tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  2:2098840/1‑146 (MQ=11)
                          tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  2:2098484/1‑146 (MQ=11)
                          tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  1:2342255/1‑146 (MQ=11)
                          tttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatg                          >  2:441915/1‑146 (MQ=11)
                           ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATgg                         <  2:3108603/148‑1 (MQ=11)
                           ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATgg                         <  2:3107474/148‑1 (MQ=11)
                           ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATgg                         <  2:2422353/148‑1 (MQ=11)
                           ttGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATgg                         <  2:3108130/148‑1 (MQ=11)
                            tGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggt                        >  1:4041272/1‑144 (MQ=11)
                            tGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggt                        <  2:4335786/148‑2 (MQ=11)
                             gTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtc                       >  2:2873429/1‑143 (MQ=11)
                             gTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAATTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtc                       >  1:1652842/1‑146 (MQ=11)
                              tCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtca                      >  2:1621126/1‑142 (MQ=11)
                               cATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                     >  1:2790231/1‑141 (MQ=11)
                               cATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCtgaagatggtcaa                     >  1:4538907/1‑141 (MQ=11)
                               cATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtcaa                     >  2:3532205/1‑144 (MQ=11)
                                aTGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtcaag                    >  2:2432982/1‑143 (MQ=11)
                                 tGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtcaaga                   >  2:4323242/1‑142 (MQ=11)
                                  gAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATggtcaagat                  >  2:3729396/1‑141 (MQ=11)
                                      aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGt              <  2:1286371/148‑1 (MQ=11)
                                      aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGt              >  1:3510149/1‑148 (MQ=11)
                                      aaaaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGt              >  1:1775250/1‑148 (MQ=11)
                                      aaaaTGTGATATAGATTCACTTTTCGCAAAATAAGATTTACTATTTTGTCGAAAGGAGATACAT‑ATA‑‑TTATGGCACATGGACCCGTAAAATGGTTTAACGCTACTAAAGGATTTGGTGTTATTACCTCTGAAGATGGTCAAGATTTGt              <  2:1936423/148‑1 (MQ=2)
                                        aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  2:422389/1‑148 (MQ=11)
                                        aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            <  2:2053657/148‑1 (MQ=11)
                                        aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  1:1324110/1‑148 (MQ=11)
                                        aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  1:3255539/1‑148 (MQ=11)
                                        aaTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTc            >  1:50559/1‑148 (MQ=11)
                                         aTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCg           <  1:1600907/148‑1 (MQ=11)
                                         aTGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCg           >  1:404189/1‑148 (MQ=11)
                                          tGTTTTATAGATTCTCTTTTCGCAGAATAAGATTTACAATTTTGTCGAAAGGAAATAGAT‑ATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGc          <  2:4310195/148‑1 (MQ=11)
                                          tGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGc          >  2:3199024/1‑148 (MQ=11)
                                           gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  2:251760/1‑148 (MQ=11)
                                           gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  2:3424884/1‑148 (MQ=11)
                                           gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         <  2:4372108/148‑1 (MQ=11)
                                           gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  2:4580368/1‑148 (MQ=11)
                                           gTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCt         >  1:1989805/1‑148 (MQ=11)
                                            ttttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTc        >  1:832813/1‑148 (MQ=11)
                                            ttttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGGAAAATGGTTTAACGCTACTAAAGGATTTGGCTTTATTACCTCTGAAGATGGTCAAGAGTTGTTCGCTc        >  1:833118/1‑148 (MQ=11)
                                            ttttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑ATATCTTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTc        >  2:1925682/1‑148 (MQ=11)
                                              ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  2:734444/1‑148 (MQ=11)
                                              ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  1:3169677/1‑148 (MQ=11)
                                              ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  1:276299/1‑148 (MQ=11)
                                              ttATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCAc      >  1:3169294/1‑148 (MQ=11)
                                               tataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACt     >  2:693467/1‑148 (MQ=11)
                                               tataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACt     >  1:1296766/1‑148 (MQ=11)
                                                ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACtt    >  1:2407566/1‑148 (MQ=11)
                                                ataGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACtt    >  2:2833499/1‑148 (MQ=11)
                                                  aGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct  >  2:4331024/1‑148 (MQ=11)
                                                  aGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct  >  1:1325423/1‑148 (MQ=11)
                                                  aGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATA‑AATATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTtct  >  2:4330350/1‑148 (MQ=11)
                                                                                                                                              |||                                                        
ATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATA‑A‑‑ATA‑‑TTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCT  >  CP043523/1848187‑1848382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑