BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,885,813	2 bp→CC	coding (39‑40/351 nt)	FTN78_chr1931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,885,813	0	T	C	99.6%	499.9 / NA	266	I13I (ATT→ATC)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (133/132); minor base A (0/1); total (133/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP043523	1,885,814	0	A	C	99.2%	503.6 / NA	266	R14R (AGG→CGG)	FTN78_chr1931	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (133/131); total (133/133)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

AAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885689‑1885931

||

  gtagtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGatga                                                                                               >  2:4354096/1‑148 (MQ=14)

     gtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGAtgatga                                                                                            >  1:4094771/1‑148 (MQ=9)

      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:2160498/148‑1 (MQ=9)

      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:3016658/148‑1 (MQ=9)

      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                           <  2:2239899/148‑1 (MQ=14)

      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                           >  1:530145/1‑148 (MQ=14)

       ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                          <  1:1696591/148‑1 (MQ=9)

        tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                         >  1:112008/1‑148 (MQ=9)

        tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                         >  1:2548492/1‑148 (MQ=9)

         aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATg                                                                                        <  1:3465910/148‑1 (MQ=9)

         aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATg                                                                                        <  1:2633136/148‑1 (MQ=9)

         aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATg                                                                                        >  2:3957817/1‑148 (MQ=14)

          tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       >  2:411461/1‑148 (MQ=9)

          tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       <  1:1212382/148‑1 (MQ=9)

          tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       >  1:2920411/1‑148 (MQ=9)

            cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:449889/141‑1 (MQ=9)

            cGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:206999/148‑1 (MQ=14)

             gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATggggc                                                                                    >  2:1231262/7‑147 (MQ=9)

             gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATggggc                                                                                    >  1:2313519/7‑147 (MQ=14)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1062907/145‑1 (MQ=9)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:4459021/145‑1 (MQ=9)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  2:243568/4‑148 (MQ=14)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1576746/145‑1 (MQ=9)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1504845/4‑148 (MQ=14)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  2:2743521/4‑148 (MQ=14)

                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1035787/4‑148 (MQ=14)

                 attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                                >  2:1154676/3‑148 (MQ=14)

                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               <  1:4353908/147‑1 (MQ=14)

                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               <  1:1761469/147‑1 (MQ=14)

                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               >  1:3158717/2‑148 (MQ=14)

                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               >  1:4345688/2‑148 (MQ=14)

                    cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:752226/148‑1 (MQ=14)

                     aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGc                                                                            >  2:3479348/1‑148 (MQ=14)

                      cGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                           >  2:4221398/1‑148 (MQ=14)

                       gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          >  2:1415097/1‑148 (MQ=14)

                       gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          <  2:4062288/148‑1 (MQ=14)

                        tgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGAc                                                                         >  2:2986703/1‑148 (MQ=14)

                           cATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgg                                                                      >  1:964130/1‑148 (MQ=14)

                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  2:1714070/148‑2 (MQ=14)

                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  1:4044225/148‑2 (MQ=14)

                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAATCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTTCGACTgga                                                                     >  2:808044/1‑147 (MQ=14)

                             tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    <  1:2322851/148‑3 (MQ=9)

                             tttATCGGAACAAACTCATTGGAGGACATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    >  2:3702487/1‑146 (MQ=255)

                                 tCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                                >  1:495335/1‑146 (MQ=14)

                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               <  2:4275548/148‑4 (MQ=9)

                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               >  2:3189738/1‑145 (MQ=14)

                                   ggAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactca                                                              >  2:2870570/1‑144 (MQ=14)

                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  1:35277/148‑7 (MQ=9)

                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  2:3553693/148‑7 (MQ=9)

                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            >  2:3764657/1‑142 (MQ=9)

                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             >  1:1363456/1‑148 (MQ=2)

                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             <  2:1993585/148‑1 (MQ=255)

                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             <  1:4088061/148‑1 (MQ=255)

                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:4681264/148‑1 (MQ=2)

                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           >  1:243556/1‑148 (MQ=2)

                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:3719186/148‑1 (MQ=2)

                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           >  2:2107114/1‑148 (MQ=2)

                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:1121449/1‑148 (MQ=2)

                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:3131464/1‑148 (MQ=9)

                                                        aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGGCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                         <  1:3350338/148‑1 (MQ=255)

                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       <  2:961564/148‑1 (MQ=9)

                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       >  2:4071376/1‑148 (MQ=9)

                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       >  1:3268670/1‑148 (MQ=9)

                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       <  2:3030589/148‑1 (MQ=9)

                                                           cagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                      <  1:3052059/148‑1 (MQ=9)

                                                            agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                     <  1:1631205/148‑1 (MQ=255)

                                                            agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                     >  1:4285257/1‑148 (MQ=9)

                                                              caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaag                                   >  1:707391/1‑148 (MQ=9)

                                                               aaGTCAAGACAAGGAGTTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                  <  2:1526055/148‑1 (MQ=9)

                                                                aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 >  2:857055/1‑148 (MQ=9)

                                                                 gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1417187/148‑1 (MQ=9)

                                                                 gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1314753/148‑1 (MQ=9)

                                                                  tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTTCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaa                               >  1:700408/1‑148 (MQ=9)

                                                                  tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaa                               <  1:387898/148‑1 (MQ=9)

                                                                   cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCAT‑GCT‑TATAACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaaaaaaa                              <  2:2577637/148‑1 (MQ=9)

                                                                   cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaaa                              <  2:2049074/148‑1 (MQ=9)

                                                                   cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaaa                              >  1:3617556/1‑148 (MQ=9)

                                                                      gACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                           >  2:4666855/1‑148 (MQ=9)

                                                                       aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          >  2:1881852/1‑148 (MQ=9)

                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGGCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGTATGTTGAAATGGGGCGCAGTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         <  1:4157984/148‑1 (MQ=255)

                                                                           ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                      <  1:1884460/148‑1 (MQ=12)

                                                                            gaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                     <  1:3827059/148‑1 (MQ=255)

                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   <  1:2410604/145‑1 (MQ=9)

                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   >  2:2323879/4‑148 (MQ=9)

                                                                               gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  >  1:2293771/3‑148 (MQ=9)

                                                                               gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  >  1:839984/3‑148 (MQ=9)

                                                                                taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 >  2:1468276/2‑148 (MQ=9)

                                                                                taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:1372082/147‑1 (MQ=255)

                                                                                taaaaGATGTCAGTTGACCGTTAATACAGCCAATATGAAGTAAT‑ACG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:618916/147‑1 (MQ=255)

                                                                                taaaaGATGGCAGTTGACCCTTCATACAGCCCCTATGAAGACAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTTGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 <  2:2794461/147‑1 (MQ=255)

                                                                                 aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                <  1:1981747/148‑2 (MQ=9)

                                                                                 aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                <  1:2648924/148‑2 (MQ=9)

                                                                                 aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGACAT‑CCG‑TGCTAATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataagaa                <  2:4549686/148‑2 (MQ=9)

                                                                                  aaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaa               <  2:1031804/148‑3 (MQ=9)

                                                                                   aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATgataagaaaa              <  1:2859944/148‑1 (MQ=9)

                                                                                    aGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAt             >  1:2720011/1‑148 (MQ=9)

                                                                                     gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            >  1:959565/1‑148 (MQ=255)

                                                                                      aTGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTc           >  2:461221/1‑148 (MQ=255)

                                                                                         tCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAg        <  2:1772092/148‑1 (MQ=255)

                                                                                               gACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTAc  <  2:2389290/148‑1 (MQ=255)

||

AAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885689‑1885931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`