BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,848,162	1 bp→CA	intergenic (+147/‑130)	FTN78_chr1897 → / → FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,848,161	1	.	C	92.0%	763.0 / 86.3	299	intergenic (+146/‑131)	FTN78_chr1897/FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base . (11/13); new base C (165/110); total (176/123)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP043523	1,848,162	0	G	A	92.3%	709.9 / 50.0	299	intergenic (+147/‑130)	FTN78_chr1897/FTN78_chr1898	Cold shock protein of CSP family/Cold shock protein of CSP family
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (166/109); minor base C (10/13); total (176/123)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 326 total aligned reads displayed.

AAAGCATGGGGTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCATTACGATATGGATTTTATTAAT‑G‑GAGAGTAGTTTGACATTGACTATAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATAA‑‑ATATTATGGCAAATGGAACAGTA  >  CP043523/1848023‑1848309

|||

  aGCATGGGGTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGagtagt                                                                                                                                              >  1:4335731/1‑148 (MQ=255)

    cATGGGGTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGttt                                                                                                                                            >  1:2722177/1‑148 (MQ=255)

    cATGGGGTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGttt                                                                                                                                            >  1:941716/1‑148 (MQ=255)

     aTGGGGTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTg                                                                                                                                           >  1:554721/1‑148 (MQ=255)

     aTGGGGTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTg                                                                                                                                           <  1:3649324/148‑1 (MQ=255)

       ggggTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGAc                                                                                                                                         >  1:35883/1‑148 (MQ=255)

          gtgtCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACAtt                                                                                                                                      >  1:1190884/1‑148 (MQ=255)

          gtgtCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACAtt                                                                                                                                      <  1:1341147/148‑1 (MQ=255)

           tgtCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAACATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTg                                                                                                                                     <  1:278053/148‑1 (MQ=255)

           tgtCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTg                                                                                                                                     >  1:4618065/1‑148 (MQ=255)

            gtCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGa                                                                                                                                    <  1:4361810/148‑1 (MQ=255)

             tCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGag                                                                                                                                   <  1:346873/148‑2 (MQ=39)

              ccTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGagt                                                                                                                                  >  1:2392199/1‑146 (MQ=38)

                attGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGtat                                                                                                                                <  1:2321826/147‑1 (MQ=38)

                  tGCTTTTTTATTTATTGCTAATGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATaa                                                                                                                              <  1:3033770/148‑1 (MQ=37)

                   gCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAAt                                                                                                                             <  1:1249215/148‑1 (MQ=38)

                   gCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAAt                                                                                                                             <  1:3775695/148‑1 (MQ=38)

                    cTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAAtt                                                                                                                            <  1:1040851/148‑1 (MQ=38)

                       ttttATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAgt                                                                                                                         >  1:3641197/1‑148 (MQ=38)

                       atttatttaTTGATCTAGCAAAAATAAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAgt                                                                                                                         <  1:4084596/147‑1 (MQ=25)

                        tttatttaTTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAgtg                                                                                                                        <  1:4502597/148‑1 (MQ=38)

                         ttatttatTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAgtgt                                                                                                                       >  1:1602452/1‑148 (MQ=38)

                         ttatttatTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAgtgt                                                                                                                       >  1:2943503/1‑148 (MQ=38)

                         ttatttatTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAgtgt                                                                                                                       <  1:1401605/148‑1 (MQ=37)

                           atttattGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGtta                                                                                                                     >  1:1109723/1‑148 (MQ=37)

                           atttattGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGtta                                                                                                                     >  1:1701119/1‑148 (MQ=37)

                           atttattGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAAATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGtta                                                                                                                     <  1:4405085/148‑1 (MQ=37)

                            tttattGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGttat                                                                                                                    <  1:2217720/148‑1 (MQ=37)

                            tttattGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGttat                                                                                                                    >  1:87161/1‑148 (MQ=37)

                            tttattGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATGTTATTAATCA‑GAGAGTAGTTTGATATTGAGTATAATTAGTGttat                                                                                                                    >  1:1286371/1‑148 (MQ=37)

                               attgctattgcAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTtattat                                                                                                                 >  1:4168950/1‑148 (MQ=37)

                               attgctattgcAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGCGTtattat                                                                                                                 >  1:3107474/1‑148 (MQ=37)

                                     attgcAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATaaagaa                                                                                                           <  1:3973112/148‑1 (MQ=37)

                                       tgcAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGt                                                                                                         >  1:3912213/1‑148 (MQ=37)

                                         cAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTag                                                                                                       >  1:716710/1‑147 (MQ=37)

                                             aaTGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGAttt                                                                                                   >  1:373258/1‑148 (MQ=25)

                                                gAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGt                                                                                                >  1:3057057/1‑148 (MQ=25)

                                                        aTGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTa                                                                                        >  1:2053657/1‑148 (MQ=25)

                                                               aCTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGtt                                                                                 >  1:1455468/1‑148 (MQ=21)

                                                                   agtgagGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTtat                                                                             >  1:1891042/1‑148 (MQ=21)

                                                                       agGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGAt                                                                         <  1:313514/148‑1 (MQ=21)

                                                                          gAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCa                                                                      >  2:1433579/1‑148 (MQ=18)

                                                                           aaaGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCAc                                                                     >  2:636328/1‑148 (MQ=18)

                                                                            aaGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTTTCATGAGTAAAATGTTTTATAGATTCACt                                                                    >  1:3218549/1‑148 (MQ=18)

                                                                             aGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACtt                                                                   >  2:2738538/1‑148 (MQ=18)

                                                                              gATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACttt                                                                  >  1:4676183/1‑148 (MQ=18)

                                                                              gATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACttt                                                                  >  2:872865/1‑148 (MQ=18)

                                                                               aTTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACtttt                                                                 <  1:2852330/148‑1 (MQ=18)

                                                                               aTTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACtttt                                                                 <  1:2640740/148‑1 (MQ=18)

                                                                                   gTTGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCa                                                             <  1:1070892/148‑1 (MQ=18)

                                                                                    ttGCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCaa                                                            >  2:3963091/1‑148 (MQ=18)

                                                                                      gCTTTCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTACTCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCaaaa                                                          >  2:4671390/1‑148 (MQ=18)

                                                                                        tttCCCTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAAta                                                        >  1:4278147/1‑147 (MQ=18)

                                                                                           cccTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATaaga                                                     >  1:2115304/1‑148 (MQ=18)

                                                                                           cccTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATaaga                                                     <  2:3035187/148‑1 (MQ=18)

                                                                                            ccTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCG‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAt                                                    <  1:3701921/148‑1 (MQ=18)

                                                                                             cTACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAtt                                                   >  1:1642850/1‑148 (MQ=18)

                                                                                              tACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAttt                                                  >  2:2064135/1‑148 (MQ=18)

                                                                                              tACAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGAttt                                                  >  2:187820/1‑148 (MQ=18)

                                                                                                cAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTAc                                                >  2:288446/1‑148 (MQ=18)

                                                                                                cAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTAc                                                >  1:1417275/1‑148 (MQ=18)

                                                                                                cAGTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTAc                                                >  2:1691920/1‑148 (MQ=18)

                                                                                                  gTTCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACaa                                              >  1:2422353/1‑148 (MQ=18)

                                                                                                   ttCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAAt                                             >  2:2076466/1‑148 (MQ=18)

                                                                                                    tCATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAAtt                                            <  2:649966/148‑1 (MQ=18)

                                                                                                     cATAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAAttt                                           <  2:3074462/148‑1 (MQ=18)

                                                                                                       tAAAATCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTg                                         <  1:337732/148‑1 (MQ=18)

                                                                                                        aaaaTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGt                                        >  1:2535275/1‑148 (MQ=18)

                                                                                                        aaaaTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGt                                        <  2:316941/148‑1 (MQ=18)

                                                                                                           aTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGa                                     >  2:1572679/1‑148 (MQ=17)

                                                                                                           aTCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGa                                     >  2:3506292/1‑148 (MQ=18)

                                                                                                           aTCCATGTCACTACGATATGGATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGa                                     <  2:1809389/148‑1 (MQ=18)

                                                                                                            tCCATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGaa                                    >  1:622782/1‑148 (MQ=17)

                                                                                                             ccATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGaaa                                   >  1:2527234/1‑148 (MQ=17)

                                                                                                             ccATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGaaa                                   >  2:3249596/1‑148 (MQ=17)

                                                                                                              cATGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTGTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAg                                  >  2:1768453/1‑148 (MQ=17)

                                                                                                               aTGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAgg                                 <  2:1701119/148‑1 (MQ=17)

                                                                                                                tGTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGa                                >  1:1156916/1‑148 (MQ=17)

                                                                                                                 gTCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaa                               >  2:2467354/1‑148 (MQ=17)

                                                                                                                  tCACTACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaa                              <  2:860046/148‑1 (MQ=17)

                                                                                                                     ctACGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa                           >  2:3418896/2‑148 (MQ=17)

                                                                                                                       aagATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑at                       >  2:4257867/3‑148 (MQ=17)

                                                                                                                       aCGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑at                       >  2:1408595/1‑148 (MQ=17)

                                                                                                                       aCGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑at                       >  1:1529357/1‑148 (MQ=17)

                                                                                                                       aCGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑at                       >  2:392069/1‑148 (MQ=17)

                                                                                                                       aCGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGaaataa‑‑at                       <  2:3753172/148‑1 (MQ=17)

                                                                                                                        cGATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAaataa‑‑ata                      <  2:554721/148‑1 (MQ=17)

                                                                                                                         gATATGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atat                     <  2:495559/148‑1 (MQ=17)

                                                                                                                           tatGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atatat                   >  1:2081646/1‑146 (MQ=14)

                                                                                                                             tGGATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atatatta                 <  2:4168950/148‑5 (MQ=14)

                                                                                                                              ggATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atatattat                >  2:4502508/1‑143 (MQ=14)

                                                                                                                              ggATTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atatattat                >  2:2207293/1‑143 (MQ=14)

                                                                                                                               gATTTTATTAAT‑CAGAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atatattatg               <  2:1281502/148‑7 (MQ=14)

                                                                                                                                aTTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atatattatgg              <  2:3663576/148‑8 (MQ=14)

                                                                                                                                aTTTTATTAATCA‑GAGAGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAA‑‑atatattatgg              <  1:807280/148‑8 (MQ=14)

                                                                                                                                        taatcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATATTATGGCAAATgg         <  2:1109723/142‑1 (MQ=14)

                                                                                                                                           tcagagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATATTATGGCAAATGGAAc      >  1:1954656/4‑148 (MQ=14)

                                                                                                                                             agagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATATTATGGCAAATGGAACAg    <  2:4316431/147‑1 (MQ=14)

                                                                                                                                              gagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATATTATGGCAAATGGAACAGt   >  2:1464244/1‑148 (MQ=14)

                                                                                                                                              gagaGTAGTTTGACATTGAGTATAATTAGTGTTATTATAAAGAAGTAGATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATAAGATTTACAATTTTGTCGAAAGGAAATAAATATATTATGGCAAATGGAACAGt   >  2:2240163/1‑148 (MQ=14)

|||

AAAGCATGGGGTGTCCTTTGCTTTTTTATTTATTGCTATTGCAAAAATGAATTAAAATGTTATACTGAGTGAGGGAAAGATTAGTTGCTTTCCCTACAGTTCATAAAATCCATGTCATTACGATATGGATTTTATTAAT‑G‑GAGAGTAGTTTGACATTGACTATAATTAGTGTTATTATGAAGAAGTAAATTTTGTCATGAGTAAAATGTTTTATAGATTCACTTTTCGCAAAATGAGATTTACAATTTTGTCGAAAGGAAATAA‑‑ATATTATGGCAAATGGAACAGTA  >  CP043523/1848023‑1848309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`