BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP043523	1,883,262	3 bp→TTA	intergenic (+143/‑16)	FTN78_chr1927 → / → FTN78_chr1928	3‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP043523	1,883,262	0	C	T	99.7%	1286.4 / NA	376	intergenic (+143/‑18)	FTN78_chr1927/FTN78_chr1928	3‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (185/190); minor base G (0/1); total (185/191)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP043523	1,883,263	0	A	T	100.0%	1290.2 / NA	376	intergenic (+144/‑17)	FTN78_chr1927/FTN78_chr1928	3‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (185/191); total (185/191)
*	CP043523	1,883,264	0	C	A	100.0%	1253.2 / NA	376	intergenic (+145/‑16)	FTN78_chr1927/FTN78_chr1928	3‑ketoacyl‑CoA thiolase, Acetyl‑CoA acetyltransferase/Transposase TnpA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (185/191); total (185/191)

Only 100 of 376 total aligned reads displayed.

AAGCCATCT‑TAGACTCCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCT  >  CP043523/1883126‑1883396

|||

 aGCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTgag                                                                                                                             <  1:1952650/148‑1 (MQ=255)

  gCCATCTATAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTgaga                                                                                                                            >  1:3870118/1‑148 (MQ=255)

    catctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGAtt                                                                                                                          <  1:4195800/142‑1 (MQ=255)

     atctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGG‑‑‑TGGAACGATTGACATGTAAAAAATCAACGCTTGAGAAAATTACTCCTTGAGAttt                                                                                                                         <  2:2238125/143‑1 (MQ=255)

      tctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTta                                                                                                                        >  2:1069200/5‑148 (MQ=255)

       ctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTtat                                                                                                                       >  2:1872222/4‑148 (MQ=255)

       ctatAGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTtat                                                                                                                       >  1:2456600/4‑148 (MQ=255)

        tatAGTCTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTtata                                                                                                                      <  2:3788792/146‑1 (MQ=255)

           aGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGa                                                                                                                   >  2:623093/1‑148 (MQ=255)

           aGACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGa                                                                                                                   >  1:3927729/1‑148 (MQ=255)

            gACTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaa                                                                                                                  <  1:4065722/148‑1 (MQ=255)

             aCTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                 >  2:238614/1‑148 (MQ=255)

             aCTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                 >  2:2994322/1‑148 (MQ=255)

             aCTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGaaa                                                                                                                 <  1:260123/148‑1 (MQ=255)

              cTCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAg                                                                                                                >  2:841135/1‑148 (MQ=255)

               tCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGc                                                                                                               >  1:452415/1‑148 (MQ=255)

               tCCGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGc                                                                                                               <  2:941046/148‑1 (MQ=255)

                ccGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGct                                                                                                              <  1:106636/148‑2 (MQ=255)

                ccGATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGct                                                                                                              >  2:3324711/1‑147 (MQ=255)

                  gATTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaa                                                                                                            <  1:794925/148‑1 (MQ=255)

                   aTTTGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGctaga                                                                                                           >  1:3307338/1‑144 (MQ=255)

                     ttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGACAGCTaagaa                                                                                                         >  1:1672403/1‑148 (MQ=255)

                     ttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaa                                                                                                         >  1:2432006/1‑148 (MQ=255)

                     ttGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaa                                                                                                         >  1:2431214/1‑148 (MQ=255)

                      tGAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaa                                                                                                        <  2:2946454/148‑1 (MQ=255)

                       gAACACACGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaa                                                                                                       >  2:3928585/1‑148 (MQ=255)

                          cacacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTaagaaaagaa                                                                                                    >  2:2375943/1‑148 (MQ=255)

                           acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                   >  1:645416/1‑148 (MQ=255)

                           acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                   >  1:3272591/1‑148 (MQ=255)

                           acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                   <  1:2943519/148‑1 (MQ=255)

                           acacGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAAt                                                                                                   <  2:4560695/148‑1 (MQ=255)

                              cGACAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTgg                                                                                                >  2:213220/1‑148 (MQ=255)

                                 cAGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAAc                                                                                             >  2:4332994/1‑148 (MQ=255)

                                  aGTTATGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACg                                                                                            >  2:2160294/1‑148 (MQ=255)

                                   gttaTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACga                                                                                           <  1:271416/145‑1 (MQ=255)

                                      aTGCTCTGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGagag                                                                                        >  1:3224965/1‑148 (MQ=255)

                                         ctctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtt                                                                                     <  1:1851087/148‑1 (MQ=255)

                                           ctGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCtttt                                                                                   <  1:179577/148‑1 (MQ=255)

                                            tGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTa                                                                                  >  1:1471202/1‑148 (MQ=255)

                                            tGTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTa                                                                                  >  1:868200/1‑148 (MQ=255)

                                             gTCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaa                                                                                 <  1:3733470/148‑1 (MQ=255)

                                              tCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaa                                                                                <  1:1007244/148‑1 (MQ=255)

                                              tCGTGTTTTACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTaaa                                                                                >  1:2918918/1‑148 (MQ=255)

                                                   ttttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAGCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAt                                                                           >  1:345573/1‑148 (MQ=255)

                                                    tttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAtt                                                                          >  1:2588326/1‑148 (MQ=255)

                                                     ttACATGTCAATCGTTCCACCTACTGTAACTTCATGAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttc                                                                         <  1:1191986/148‑1 (MQ=255)

                                                     ttACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttc                                                                         >  1:1917174/1‑148 (MQ=255)

                                                      tACATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttct                                                                        <  2:2594846/148‑1 (MQ=255)

                                                       aCATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttctt                                                                       <  2:3552430/148‑1 (MQ=255)

                                                       aCATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttctt                                                                       <  2:2955751/148‑1 (MQ=255)

                                                       aCATGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttctt                                                                       >  1:2207757/1‑148 (MQ=255)

                                                         aTGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATtcttct                                                                     >  1:319211/1‑148 (MQ=255)

                                                          tGTCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTg                                                                    >  1:4463314/1‑148 (MQ=255)

                                                           gTCAACCGTGCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCAttcttggag                                                                   <  2:4232968/148‑5 (MQ=255)

                                                            tCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                  <  2:4113841/148‑1 (MQ=255)

                                                            tCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                  >  1:2266049/1‑148 (MQ=255)

                                                            tCAATCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAAGTACTCCTTGAGATTTATATGAAAGCTCAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCg                                                                  <  1:3061301/148‑1 (MQ=255)

                                                              aaTCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTg                                                                <  2:1617006/148‑1 (MQ=255)

                                                                tCGTTCCACCTACTGTAACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGAt                                                              >  1:995632/1‑148 (MQ=255)

                                                                        cctactgtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGt                                                      >  1:931894/8‑148 (MQ=255)

                                                                         ctactgtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGtt                                                     >  2:4042082/7‑148 (MQ=255)

                                                                          tactgtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTa                                                    <  1:139328/143‑1 (MQ=255)

                                                                           actgtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTaa                                                   >  2:1643163/5‑148 (MQ=255)

                                                                           actgtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTaa                                                   >  2:673002/5‑148 (MQ=255)

                                                                             tgtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAata                                                 <  1:570779/146‑1 (MQ=255)

                                                                              gtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAatat                                                <  1:3162298/147‑1 (MQ=255)

                                                                              gtaACTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAatat                                                <  1:3667068/147‑1 (MQ=255)

                                                                                  cTTCATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCt                                            <  1:718864/148‑1 (MQ=255)

                                                                                    tcataAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGa                                          <  1:841408/146‑1 (MQ=255)

                                                                                     cataAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGaa                                         >  1:1144367/2‑148 (MQ=255)

                                                                                      ataAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAg                                        >  2:2686395/1‑148 (MQ=255)

                                                                                       taAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAgg                                       >  2:1236518/1‑148 (MQ=255)

                                                                                        aaaCAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGc                                      >  1:1942507/1‑148 (MQ=255)

                                                                                          aCAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCCACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGccg                                    <  2:3677098/148‑3 (MQ=255)

                                                                                          aCAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGccg                                    <  1:3512714/148‑3 (MQ=255)

                                                                                          aCAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGccg                                    <  2:3186264/148‑3 (MQ=255)

                                                                                            aaGAGGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTa                                  <  1:4605380/148‑1 (MQ=255)

                                                                                             agagGCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTAt                                 <  2:3236854/148‑1 (MQ=255)

                                                                                                ggCCTTCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCtt                              <  2:2791621/148‑1 (MQ=255)

                                                                                                    ttCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGa                          >  2:2041905/1‑148 (MQ=255)

                                                                                                     tCGAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGAc                         <  1:4647744/148‑1 (MQ=255)

                                                                                                       gAA‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACtt                       >  2:3033067/1‑148 (MQ=255)

                                                                                                        aa‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtc                      <  1:2355181/148‑1 (MQ=255)

                                                                                                         a‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtct                     <  2:3505625/148‑1 (MQ=255)

                                                                                                         a‑GCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtct                     <  1:2070347/148‑1 (MQ=255)

                                                                                                           gCGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTtctc                    >  2:2521151/1‑148 (MQ=255)

                                                                                                            cGTGA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCa                   >  2:478487/1‑148 (MQ=255)

                                                                                                              agA‑A‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAg                 >  2:3632401/2‑148 (MQ=255)

                                                                                                                a‑a‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTc               <  2:2836123/148‑1 (MQ=255)

                                                                                                                  a‑GTAGAAAATCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCt              <  2:145635/148‑1 (MQ=255)

                                                                                                                       gaaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGa          >  1:1764908/2‑148 (MQ=255)

                                                                                                                        aaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGac         <  1:2124770/148‑1 (MQ=255)

                                                                                                                        aaaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGac         <  1:1602195/148‑1 (MQ=255)

                                                                                                                         aaaTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGaca        <  2:272421/148‑1 (MQ=255)

                                                                                                                           aTCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACt      >  2:4364522/1‑148 (MQ=255)

                                                                                                                            tCAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTc     >  2:1273426/1‑148 (MQ=255)

                                                                                                                             cAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTcc    >  2:1639638/1‑148 (MQ=255)

                                                                                                                             cAACGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTcc    >  2:598687/1‑148 (MQ=255)

                                                                                                                              aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTccc   <  2:2744453/148‑1 (MQ=255)

                                                                                                                              aaCGCTTGAGAAAATTACTCCTTGAGATTTATATGAAAGCTAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCCGTATCTTAAGACTTCTCAAGTCTATGACACTccc   >  2:1802336/1‑148 (MQ=255)

|||

AAGCCATCT‑TAGACTCCGATTTGAACACACGACAGTCATGCTCTGTCGTGTTTTACATGTCAATCGTTCCATCAACTATAACTTTATAAACAAGAGGCCTTCGAA‑GCGTGA‑A‑GTAAAAAATCAACGCTTGTGAAAACACCTCCTTGAGATTTATATGAAAGCCAAGAAAAGAATTGGAACGAGAGCTTTTAAAATCATTCTTCTGCGTGATTATGGCGTTAATATTTCTGAAGGCTGTATCTTAAGACTTCTCAAGTCTATGACACTCCCT  >  CP043523/1883126‑1883396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`