Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,104,332	G→A	P215L (CCA→CTA)	cpxA ←	sensor histidine kinase CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,104,332	0	G	A	100.0%	131.4 / NA	38	P215L (CCA→CTA)	cpxA	sensor histidine kinase CpxA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/19);  total (19/19)

ATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATCACGCACGGAGAACGGACCGACCAGTTCCACGCGG  >  NC_000913/4104085‑4104604                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                  aTTACGTGAACACACCAACAGATCGTTGATCATGCTGGCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTcc                                                                                                                                                                                                                                              <  1:197340/285‑1 (MQ=255)         aCATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAg                                                                                                                                                                                                                                                   <  1:210707/272‑1 (MQ=255)              aCCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAg                                                                                                                                                                                                                                                   <  1:130866/267‑1 (MQ=255)                  aCAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCa                                                                                                                                                                                                                                       <  1:575142/275‑1 (MQ=255)                    aGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATACCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCCCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTTCGGCCAGGAATTCCTTGAGCNCCGCGTCCTGGTCGGGGTgggnnggn                                                                                                                                                                                                                                                      >  2:130866/1‑251 (MQ=255)                                           aCGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGCGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGATAAAACTGGCACCTGCGGCAAGGAATTCCTGTACCCCCGCCTCCGGTTCCGGGGTCTGGGCTAAGGTTGCCTGGGCCACTTCCTTGGGCGCGGTTTTTCTGCtcccgctcg                                                                                                                                                                                                     >  2:239757/1‑276 (MQ=255)                                                                 nnncgcgttgccTGGCTTCCGCCGCTACGCCGGCGAAGTAACGCCGGACCCAGTGGCAGACGAGTCAGCGGGGTGCGCAGGTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAAc                                                                                                                                                                             <  2:450785/275‑1 (MQ=255)                                                                                   tccccGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCAGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTa                                                                                                                                                           <  1:239757/282‑1 (MQ=255)                                                                                               cgGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAg                                                                                                                                                                                                                          <  2:282240/211‑1 (MQ=255)                                                                                               cgGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAg                                                                                                                                                                                                                          >  1:282240/1‑211 (MQ=255)                                                                                                       tAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGTCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATgggaaaaat                                                                                                                                       >  1:355888/1‑278 (MQ=255)                                                                                                         aagCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGAACATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGTGTGCTGGCGTAAGTTGCCCTGTGCAACTTCCTAGGCAGC‑CTTTTTCAGCTTACGCGCCGGTTTTGACAGACTCCAGGCCAACCACAACAGCAgcggcg                                                                                                                                                             <  1:25340/259‑1 (MQ=255)                                                                                                            ccGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCa                                                                                                                                                                                           <  1:115324/228‑1 (MQ=255)                                                                                                            ccGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCa                                                                                                                                                                                           >  2:115324/1‑228 (MQ=255)                                                                                                               tACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCATGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTc                                                                                                                                                                                      >  1:503389/1‑230 (MQ=255)                                                                                                                 cccAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTc                                                                                                                                                                                      >  1:373117/1‑228 (MQ=255)                                                                                                                 cccAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAAc                                                                                                                                                                             <  1:194171/237‑1 (MQ=255)                                                                                                                 cccAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAAc                                                                                                                                                                             >  2:194171/1‑237 (MQ=255)                                                                                                                            aCGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGGAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGGGACAATCAGCAGTAATAGCGGGCGGTc                                                                                                                  >  1:225600/1‑285 (MQ=255)                                                                                                                                 tCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAg                                                                                                                                                                                                                                                   >  2:133704/1‑152 (MQ=255)                                                                                                                                 tCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAg                                                                                                                                                                                                                                                   <  1:133704/152‑1 (MQ=255)                                                                                                                                   aGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGAGGTGACCATCTGGTTAAAACTGTCACCTCCGGCAAGGAATTCCTGTAGCGCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCTAGGCCAACCACAACAGCAGCGGCGTACTGCCCAACATAGTGACAACcagccgcaa                                                                                                                              >  1:420567/1‑261 (MQ=255)                                                                                                                                      ggggTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcag                                                                                                                                    <  1:29270/257‑1 (MQ=255)                                                                                                                                          tGCGCAGCTCGTGAGAGATATCAAAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTaa                                                                                                    >  1:396352/1‑285 (MQ=255)                                                                                                                                                    gTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCAAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCGGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTGTTTCAGCTTACGCGCCGGTTTTGCCAGACTCGATGCCAACCACAACAGCAGCGGCGTACTGACCCACATGGTTACACTCAGCAGTAATAGCGGGCGGTCAAACAGTACGTTAATGAAATCGGa                                                                                          >  1:282636/1‑285 (MQ=255)                                                                                                                                                              tCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTAAGCTTACGCGCCGGTTTTGCAAGACTCCAGGCCAACCACAAcag                                                                                                                                                                     <  1:326706/200‑1 (MQ=255)                                                                                                                                                                                       tcatcaTGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAgcg                                                                                                                                                                >  1:461074/1‑180 (MQ=255)                                                                                                                                                                                       tcatcaTGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAgcg                                                                                                                                                                <  2:461074/180‑1 (MQ=255)                                                                                                                                                                                                            tGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAgcg                                                                                                                                                                >  2:542020/1‑159 (MQ=255)                                                                                                                                                                                                            tGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAgcg                                                                                                                                                                <  1:542020/159‑1 (MQ=255)                                                                                                                                                                                                              aCCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATc                                                                                                                                      >  1:452405/1‑183 (MQ=255)                                                                                                                                                                                                              aCCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTGTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATc                                                                                                                                      <  2:452405/183‑1 (MQ=255)                                                                                                                                                                                                                          aaaCTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGAAGAATAAGATAAAGt                                                        >  1:209922/1‑249 (MQ=255)                                                                                                                                                                                                                           gaCTTGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTGCCGGGTGCGGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCATCATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTCATGAAATCGGATTGAGAACTGCTGGCCGTACGAATCAGATAAAGTTAGTAATTATCTTCGCCATCACGCACGGAGAACGga                    <  1:126093/283‑1 (MQ=255)                                                                                                                                                                                                                           aaCTGGCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAAGCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAAGTATCTTCGCCATCACGCACGGAGAACGga                    <  1:155134/284‑1 (MQ=255)                                                                                                                                                                                                                                gCACCTGCGGCAAGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCAAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTCATGAAATCGGATTGAGACCTGCTGGCCGGACGANACAGACAAAGTTGGaaacacg                                              >  2:126093/1‑249 (MQ=255)                                                                                                                                                                                                                                         gCAAAGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATCACGCACGGAGAACGGACCGACCAGTTCCAcg     <  1:144637/285‑1 (MQ=255)                                                                                                                                                                                                                                            aGGAATTCCTGTAGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCGGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATCACGCACGGAGAACGGACCGACCAGTTCCACGCgg  >  1:491845/1‑285 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                  ATTACGTGACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGC‑GTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATCACGCACGGAGAACGGACCGACCAGTTCCACGCGG  >  NC_000913/4104085‑4104604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑