Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,213,434	0	G	A	35.0%	61.4 / 53.5	60	V130I (GTT→ATT)	rpoD	RNA polymerase sigma factor RpoD
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base A (10/11);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACT  >  NC_000913/3213167‑3213717                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             atcGAAGACATCATCCAAATGATCAACGACATGGTCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCATGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGaa                                                                                                                                                                                                                                                                          >  1:616457/1‑288 (MQ=255)                  aaTGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATc                                                                                                                                                                                                                                                           >  1:22742/1‑286 (MQ=255)                               aTGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGaa                                                                                                                                                                                                                                            <  1:271321/288‑1 (MQ=255)                                    agggaggggaagggAAGGAGAACGGGATGTCGTTGAACTGAAGAGTGCGTAAAGAACAGCGGATCAGGAAGCGGCCGGAGGCGCTGCACAGGTGCTTTCCAGCTTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGGTGACCCGCGAAGGCGAAATTGACATCGATAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTa                                                                                                                                                                                                                                       <  2:776727/276‑1 (MQ=255)                                            tGATAGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTa                                                                                                                                                                                                                                                      <  1:119008/265‑1 (MQ=255)                                                   agaagCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAg                                                                                                                                                                                                                         <  1:649202/287‑1 (MQ=255)                                                            ggATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGaagaag                                                                                                                                                                                                                >  1:229215/1‑287 (MQ=255)                                                                            aTGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGGTGACCCGCTAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACCGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTg                                                                                                                                                                                               >  1:752274/1‑288 (MQ=255)                                                                                  gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCTAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACg                                                                                                                                                                                                                                     >  1:339337/1‑244 (MQ=255)                                                                                  gctgAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCACCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCGTGTACATGCGTGAAATGGTCTCC‑GTTGAACTGTTGACCCGCGAAGGCGAAAGTGACATCGCTAAGCGTCTTTAACCCGGGACCAACCAGGt                                                                                                                                                                                                                                                                                             >  2:192360/1‑188 (MQ=255)                                                                                            ccGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGa                                                                                                                                                                                                                                                                           >  1:309201/1‑196 (MQ=255)                                                                                            ccGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGa                                                                                                                                                                                                                                                                           <  2:309201/196‑1 (MQ=255)                                                                                             cgcgGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTAACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGAACACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTttgttg                                                                                                                                                                               >  1:515034/1‑287 (MQ=255)                                                                                                ggACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGAccc                                                                                                                                                                           >  1:841012/1‑288 (MQ=255)                                                                                                           ctgcCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGa                                                                                                                                                                                                                                                                           <  1:589638/181‑1 (MQ=255)                                                                                                            tgcCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCACTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCagaaga                                                                                                                                                               <  1:831524/288‑1 (MQ=255)                                                                                                             gcCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGCACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCGGAACGCagaaga                                                                                                                                                               <  1:528667/287‑1 (MQ=255)                                                                                                                                       cAGCGTGGAATTTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGt                                                                                                                                    <  1:175434/288‑1 (MQ=255)                                                                                                                                             ggAATCTGAAATCGGGCCTACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGTTCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGtt                                                                                                                               <  1:326997/287‑1 (MQ=255)                                                                                                                                               aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGAt                                                                                                                                                                                                                                                            >  1:277957/1‑160 (MQ=255)                                                                                                                                               aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGAt                                                                                                                                                                                                                                                            <  2:277957/160‑1 (MQ=255)                                                                                                                                                                      gACCCGGTACGCATGTACATGCGTGAAATGGGCACG‑GTTGAACTGTTTACTCGCGGAGGCGTAATTGACATCGCTAAGCATATCGAAGACGGGATCAGCCAGGTACAATGCTCCGTTGCTGTATATCCGGAAGCGATCACCTGTCTGCTGGAACAGTATGATCGTGTTGAAGCAGAAGACGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGAACCGAACGCAGAAGAAGATCTGGc                                                                                                                                                      >  1:501951/1‑239 (MQ=255)                                                                                                                                                                       aCCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGaagaag                                                                                                                                                                                                                >  2:208767/1‑180 (MQ=255)                                                                                                                                                                       aCCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGaagaag                                                                                                                                                                                                                <  1:208767/180‑1 (MQ=255)                                                                                                                                                                        cccGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGATCTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCa                                                                                                                                           <  1:128366/248‑1 (MQ=255)                                                                                                                                                                            gTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGCAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCgg                                                                                                                                                                                                                                                                   >  1:814113/1‑124 (MQ=255)                                                                                                                                                                            gTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATTCGGAAGCGATCATCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAATAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCTGAACGCagaagtag                                                                                                                                                             >  1:244057/1‑226 (MQ=255)                                                                                                                                                                            gTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTa                                                                                                                                                                                                                                                      >  1:627003/1‑137 (MQ=255)                                                                                                                                                                            gTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTa                                                                                                                                                                                                                                                      <  2:627003/137‑1 (MQ=255)                                                                                                                                                                            gTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCgg                                                                                                                                                                                                                                                                   <  2:814113/124‑1 (MQ=255)                                                                                                                                                                               agaagagtaaggCGGGAAATGGACCCCGGTTAAACGGTTGACCCCAGAAGGCGAAATTGACTTCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACg                                                                                              <  2:359344/276‑1 (MQ=255)                                                                                                                                                                                       cATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGTCATCGCTAAGCGTATTGAAGACGGGATCGACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTa                                                                                                                                                                                                                                       <  1:338521/141‑1 (MQ=255)                                                                                                                                                                                       cATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTa                                                                                                                                                                                                                                       >  2:338521/1‑141 (MQ=255)                                                                                                                                                                                       cATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGAt                                                                                                                                                                                                                                                            >  1:16501/1‑120 (MQ=255)                                                                                                                                                                                       cATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGAt                                                                                                                                                                                                                                                            <  2:16501/120‑1 (MQ=255)                                                                                                                                                                                           cGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTgg                                                                                                                                                                                                                                              <  2:335994/130‑1 (MQ=255)                                                                                                                                                                                           cGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTgg                                                                                                                                                                                                                                              >  1:335994/1‑130 (MQ=255)                                                                                                                                                                                           cGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGcgcg                                                                                                                                                                                                            <  1:547876/164‑1 (MQ=255)                                                                                                                                                                                           cGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTACCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGcgcg                                                                                                                                                                                                            >  2:547876/1‑164 (MQ=255)                                                                                                                                                                                              gAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAg                                                                                     >  1:359344/1‑280 (MQ=255)                                                                                                                                                                                               aaaTGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGTAATCGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACTTCGGTTCTGAGCTTTCCCAGGAAGACCTGGACGATGACGAATATGAAA‑ACgaagaa                                                                            >  1:564009/1‑287 (MQ=255)                                                                                                                                                                                                  tGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAgg                                                                                                                >  1:547893/1‑249 (MQ=255)                                                                                                                                                                                                  tGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGGCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAgg                                                                                                                <  2:547893/249‑1 (MQ=255)                                                                                                                                                                                                               aaCTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTGGTTGACCCGAACGCAGAAGAAGAACTGGCACCTACCGCCACCCACGTCGGTTCTGAGTCTTGCCAGGAAGTACCGGACGATGACGGACATGAAGTAGGAGGAAGAGGGGGAGgacgag                                                             >  2:317281/1‑287 (MQ=255)                                                                                                                                                                                                                      gACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATGACGACAGCGCCga                                                     >  1:13732/1‑288 (MQ=255)                                                                                                                                                                                                                          cgcgAAGGCGAAATTGACATCGCTAAGCGGATTGAGAACAGGATAAACCAGATTAAATGCTCCGTTGCAGAATACACGGAAACGATCACCCATCTGCTGAAACAGTACGATCGTGTGGAAGAAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCGACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATGACGACAGCGCCgatgat                                                 <  1:330650/288‑1 (MQ=255)                                                                                                                                                                                                                            cgAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCgtgt                                                                                                                                                                                                                              >  1:659245/1‑114 (MQ=255)                                                                                                                                                                                                                            cgAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCgtgt                                                                                                                                                                                                                              <  2:659245/114‑1 (MQ=255)                                                                                                                                                                                                                                       ttGACAACGCTAAGCGTATTGAAGACGGGATCAACCAGATTAAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGTTCGTGTTGAAGAAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCAt                                      <  1:272226/286‑1 (MQ=255)                                                                                                                                                                                                                                          acacCGCAAAGCGTATTGAAGACGGAATCACCCAGATTCAATGCTACGTTGCTGAAGATCCGGAAGCGATCACCTATCTGCTGAAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATATGGACGAGGACGAAGATGAAG‑ACAAAGAAGATGGCGATGCCGACAGCGCCGATGATGACAACAGCATCGAcc                                 <  1:232133/288‑1 (MQ=255)                                                                                                                                                                                                                                               gCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTttg                                                                                                                                                                                  >  2:493705/1‑139 (MQ=255)                                                                                                                                                                                                                                               gCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTttg                                                                                                                                                                                  <  1:493705/139‑1 (MQ=255)                                                                                                                                                                                                                                                         ttGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCg                                                                                                                                                                          >  1:593810/1‑137 (MQ=255)                                                                                                                                                                                                                                                         ttGAAGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCg                                                                                                                                                                          <  2:593810/137‑1 (MQ=255)                                                                                                                                                                                                                                                             aGACGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCAt                                      <  1:230583/264‑1 (MQ=255)                                                                                                                                                                                                                                                               aCGGGATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGAACGTGTTGAAGCAGAAGAAGCACGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGc                                        >  1:135909/1‑260 (MQ=255)                                                                                                                                                                                                                                                                  ggATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTc                                                                                                                     <  1:215027/181‑1 (MQ=255)                                                                                                                                                                                                                                                                  ggATCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTc                                                                                                                     >  2:215027/1‑181 (MQ=255)                                                                                                                                                                                                                                                                    aTCAACCAGATTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTTTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCCCGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATTACGACAGCGCCGAGGATGACAACAGCAt                                      <  1:475393/257‑1 (MQ=255)                                                                                                                                                                                                                                                                         ccATGTTTAATGCTTTGTTTCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATATGATCACCGGCTTTGTTGACCCGAACGAAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAATAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGAACCGGAACTGGCTCGCGAAAAATTTGCGGAACt  <  1:513730/288‑1 (MQ=255)                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             ATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGAAAACACCGCGGACGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACC‑GTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTGAATATCCGGAAGCGATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAG‑ACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACT  >  NC_000913/3213167‑3213717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑