New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3669833 =	66 (1.940)	3 (0.090)	3/402	3.9	4.4%	coding (242/1650 nt)	treF	cytoplasmic trehalase
	?	NC_000913	3669886 =	66 (2.000)					coding (295/1650 nt)	treF	cytoplasmic trehalase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCGCTAAAACGGCCGCCCGGGACAATATATGACTGCGGCAGCGCCAGCAGAGAAGACCACGGAATGTGATCCTGTGGTTCGCGGGTTAGCACCGGCCACAGCTGGTCGATATGCTCTTTCAGGGAATTTTGCGGGTCCGATACATACTCGCTGGAGTAGACCTCCGGCAGCCAGAAGTGGTTTTCAACAAACTTGCGCAAGTCAAAATCACGATGACGGCGCACTTTACGGTAGCGGATTAAGATATCCAGCGGGTCCATTTTAGGTGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3670104‑3669833 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgcgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAGTCATATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATTGGGATTCCTATTTCACCATGCTGGGGCTGGCGGAAAGTGGTCGGGAAGATTTG  >  NC_000913/3669886‑3670167                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            TTCGCTAAAACGGCCGCCCGCGACAATATATGACTGCGGCAGCGCCAGCAGAGAAGACCACGGAATGTGATCCTGTGGTTCGCGGGTTAGCACCGGCCACAGCTGGGCGATATGCTCTTTCAGGGAATTTTGCGGGTCCGATACATACTCGCTGGAGTAGACCTCCGGCAGCCAGAAGTGGTTTTCAACAAACTTGCGCAAGTCAAAATCACGATGACGGCGCACTTTACGGTAGCGGATTAAGACATCCAGCGGGTCCATTTTAGGTCCGCCGTtgtgaaaggt                                                                                                                                                                                                                                                                               >  1:113232/1‑275                                                                                                                                                                                                                                            CGGATTAAGATATCCAGCGGGTCCATTTTAGGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAG                                                                                                                                                                                                                        <  1:115254/105‑1                                                                                                                                                                                                                                            CGGATTAAGATATCCAGCGGGTCCATTTTAGGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAG                                                                                                                                                                                                                        >  2:115254/1‑105                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCAGTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTAT                                                                                                                                                                                                             >  1:285192/1‑82                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACCCGCGCACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAGTCATATATTGTCCCGGGCGGCCGTTTTAGCGAA                                                                 >  2:84831/1‑222                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACA                                                                                                                                          >  1:500923/1‑149                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACA                                                                                                                                          <  2:500923/149‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACA                                                                                                                                          >  1:257847/1‑149                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACA                                                                                                                                          <  2:257847/149‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACACGCGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAGTCATATATTGTCCCGGGCGGCCGTTTTAGCGAA                                                                 <  1:84831/222‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGC                                                                                                                                                                <  2:56524/127‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGC                                                                                                                                                                >  1:56524/1‑127                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAC                                                                                                                                                                          >  1:328867/1‑117                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAC                                                                                                                                                                          <  2:541418/117‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAC                                                                                                                                                                          <  2:328867/117‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAC                                                                                                                                                                          >  2:259960/1‑117                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAC                                                                                                                                                                          >  1:541418/1‑117                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAC                                                                                                                                                                          <  1:259960/117‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTG                                                                                                                                                                                         <  1:58277/102‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTG                                                                                                                                                                                         >  2:58277/1‑102                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGGATGTATCGCACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACTCGCGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAGTCCTATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATCGGTATTCCTTTTTCCCCAGTCTGGGGCTGGCGGAAAGTGGTCGGGCAGtgtgg  >  1:195925/1‑280                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGG                                                                                                                                                                                                                                     <  2:353143/58‑1                                                                                                                                                                                                                                                                              CGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGG                                                                                                                                                                                                                                     >  1:353143/1‑58                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            TTCGCTAAAACGGCCGCCCGGGACAATATATGACTGCGGCAGCGCCAGCAGAGAAGACCACGGAATGTGATCCTGTGGTTCGCGGGTTAGCACCGGCCACAGCTGGTCGATATGCTCTTTCAGGGAATTTTGCGGGTCCGATACATACTCGCTGGAGTAGACCTCCGGCAGCCAGAAGTGGTTTTCAACAAACTTGCGCAAGTCAAAATCACGATGACGGCGCACTTTACGGTAGCGGATTAAGATATCCAGCGGGTCCATTTTAGGTGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3670104‑3669833 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgcgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAGTCATATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATTGGGATTCCTATTTCACCATGCTGGGGCTGGCGGAAAGTGGTCGGGAAGATTTG  >  NC_000913/3669886‑3670167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.