BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,873,627	Δ1 bp	coding (506/672 nt)	yidX →	putative replicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,873,625	0	G	.	99.4%	1419.5 / ‑1.2	309	coding (504/672 nt)	yidX	putative replicase
Reads supporting (aligned to +/- strand): ref base G (0/2); new base . (173/134); total (173/136)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

AAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGTA  >  NC_000913/3873533‑3873713

  aaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCgga                                                                                        >  2:5983304/1‑92 (MQ=255)

  aaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCgga                                                                                        >  2:3250601/1‑92 (MQ=255)

  aaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCgga                                                                                        <  2:7097633/93‑2 (MQ=255)

  aaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCgga                                                                                        >  2:4045010/1‑92 (MQ=255)

  aaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCgga                                                                                        <  1:2260469/93‑2 (MQ=255)

   aaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCggat                                                                                       <  2:4370370/93‑3 (MQ=255)

   aaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCggat                                                                                       <  2:3762342/93‑3 (MQ=255)

   aaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCggat                                                                                       <  2:4637702/93‑3 (MQ=255)

    aTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCggatg                                                                                      <  1:634919/93‑4 (MQ=255)

       aaaaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGgtg                                                                                  >  2:1724002/1‑93 (MQ=255)

         aaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGtt                                                                                <  1:5343870/93‑1 (MQ=255)

         aaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGtt                                                                                <  1:6251582/93‑1 (MQ=255)

         aaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGtt                                                                                <  1:1142024/93‑1 (MQ=255)

         aaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGtt                                                                                <  1:4394498/93‑1 (MQ=255)

         aaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGtt                                                                                >  1:7392913/1‑93 (MQ=255)

           cATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTc                                                                              <  1:4758488/93‑1 (MQ=255)

           cATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTc                                                                              >  2:6124249/1‑93 (MQ=255)

            aTGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGTTTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTcc                                                                             <  1:7130640/93‑1 (MQ=255)

            aTGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTct                                                                             >  2:1027085/1‑92 (MQ=255)

            aTGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTcc                                                                             <  1:3107438/93‑1 (MQ=255)

             tGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCg                                                                            >  2:5460571/1‑93 (MQ=255)

               gATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCgca                                                                          <  2:4122621/93‑1 (MQ=255)

               gATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCgca                                                                          <  2:1756524/93‑1 (MQ=255)

                 ttACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCgcagc                                                                        >  1:5729853/1‑93 (MQ=255)

                 ttACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCgcagc                                                                        >  2:2733407/1‑93 (MQ=255)

                  tACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCgcagca                                                                       >  1:3029719/1‑93 (MQ=255)

                   aCTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCAt                                                                      >  1:4486385/1‑93 (MQ=255)

                   aCTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCAt                                                                      >  1:5447838/1‑93 (MQ=255)

                    cTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCAtt                                                                     >  1:859334/1‑93 (MQ=255)

                     tCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTa                                                                    <  2:5406146/93‑1 (MQ=255)

                     tCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTa                                                                    >  2:5930210/1‑93 (MQ=255)

                      cAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGTCATTCC‑GGATGGTGTTTCCGCAGCATTAc                                                                   <  2:706743/93‑1 (MQ=255)

                        aaGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTg                                                                 >  2:2454171/1‑93 (MQ=255)

                        aaGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTg                                                                 >  2:6230088/1‑93 (MQ=255)

                        aaGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTg                                                                 >  1:177194/1‑93 (MQ=255)

                         aGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGc                                                                <  1:1036764/93‑1 (MQ=255)

                         aGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGc                                                                >  1:941672/1‑93 (MQ=255)

                            tGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAg                                                             <  1:3988317/93‑1 (MQ=255)

                               tGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATT‑CGGGATGGTGTTTCCGCAGCATTACTGCCAGTGa                                                          <  2:3934619/93‑1 (MQ=255)

                                ggCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGAc                                                         <  2:2363143/93‑1 (MQ=255)

                                ggCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGAc                                                         <  1:2704544/93‑1 (MQ=255)

                                 gCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACt                                                        >  2:6441924/1‑93 (MQ=255)

                                   gTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGt                                                      <  1:2386774/93‑1 (MQ=255)

                                    ttCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGtt                                                     >  2:4976270/1‑93 (MQ=255)

                                    ttCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGtt                                                     >  1:3405713/1‑93 (MQ=255)

                                    ttCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGGGTTTCCGCAGCATTACTGCCAGTGACTGtt                                                     >  1:583184/1‑93 (MQ=255)

                                     tCTACCAACCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTa                                                    <  1:5688286/93‑1 (MQ=255)

                                       tACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACg                                                  >  1:6163478/1‑93 (MQ=255)

                                       tACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACg                                                  >  1:4672965/1‑93 (MQ=255)

                                        aCCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGc                                                 >  1:2002816/1‑93 (MQ=255)

                                         ccatccatTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCt                                                <  2:5287955/93‑1 (MQ=255)

                                         ccatccatTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCt                                                >  2:6628706/1‑93 (MQ=255)

                                           atccctTTAAAGTGCGCCTTTATGAATACTATTCACCCAGACGCATTCC‑GGCTGGGGTTTACGCAGCATGACTCCCAGTCCCTGTTACGCTgg                                              >  1:681029/1‑93 (MQ=255)

                                           atccatTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTgg                                              <  1:4150275/93‑1 (MQ=255)

                                           atccatTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTgg                                              >  1:4901931/1‑93 (MQ=255)

                                           atccatTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTgg                                              >  2:4847102/1‑93 (MQ=255)

                                            tccatTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGa                                             >  1:3767456/1‑93 (MQ=255)

                                            tccatTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGa                                             >  2:825736/1‑93 (MQ=255)

                                              catTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAca                                           >  1:1074143/1‑93 (MQ=255)

                                              catTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAca                                           <  1:1869102/93‑1 (MQ=255)

                                               atTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcat                                          <  2:1403476/93‑1 (MQ=255)

                                                tttAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatc                                         >  2:151930/1‑93 (MQ=255)

                                                tttAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatc                                         <  1:1940116/93‑1 (MQ=255)

                                                tttAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatc                                         >  2:3682250/1‑93 (MQ=255)

                                                 ttAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatca                                        >  2:2003671/1‑93 (MQ=255)

                                                    aaGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTa                                     >  2:6910882/1‑93 (MQ=255)

                                                     aGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTTTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTAc                                    >  2:92556/1‑93 (MQ=255)

                                                     aGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTAc                                    <  2:5887089/93‑1 (MQ=255)

                                                      gTGCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACt                                   >  1:4223393/1‑93 (MQ=255)

                                                        gCGATTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGc                                 <  2:4397696/93‑1 (MQ=255)

                                                           aTTTTATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCAcc                              >  2:4746524/1‑93 (MQ=255)

                                                             tttATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGc                            <  1:6035268/93‑1 (MQ=255)

                                                              ttATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCt                           <  1:1517340/93‑1 (MQ=255)

                                                              ttATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCt                           >  2:5343508/1‑93 (MQ=255)

                                                               tATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTg                          >  1:2849501/1‑93 (MQ=255)

                                                               tATGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTg                          >  2:3648308/1‑93 (MQ=255)

                                                                aTGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGc                         <  1:5934279/93‑1 (MQ=255)

                                                                 tGAATACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCa                        <  1:505625/93‑1 (MQ=255)

                                                                   aaTACTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAAt                      >  2:1258512/1‑93 (MQ=255)

                                                                      aCTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTc                   >  1:1363395/1‑93 (MQ=255)

                                                                      aCTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTc                   >  2:3028187/1‑93 (MQ=255)

                                                                       cTATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCt                  >  2:4568634/1‑93 (MQ=255)

                                                                        tATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTg                 <  2:807464/93‑1 (MQ=255)

                                                                        tATTCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTg                 >  1:3592709/1‑93 (MQ=255)

                                                                          ttCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGt               <  1:6556986/93‑1 (MQ=255)

                                                                          ttCACCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGt               <  1:2960518/93‑1 (MQ=255)

                                                                             aCCCAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGt            <  2:878596/93‑1 (MQ=255)

                                                                              cccAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGta           >  2:5546017/1‑93 (MQ=255)

                                                                              cccAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGta           <  2:639553/93‑1 (MQ=255)

                                                                               ccAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGtat          >  2:5746860/1‑93 (MQ=255)

                                                                               ccAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGtat          >  1:3331168/1‑93 (MQ=255)

                                                                                cAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGtata         <  1:3161621/93‑1 (MQ=255)

                                                                                cAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGtata         <  1:374626/93‑1 (MQ=255)

                                                                                cAGAGGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGtata         >  1:5180055/1‑93 (MQ=255)

                                                                                 agagGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTatat        <  1:253939/93‑1 (MQ=255)

                                                                                 agagGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTatat        <  2:3282208/93‑1 (MQ=255)

                                                                                  gagGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATg       <  1:6415315/93‑1 (MQ=255)

                                                                                  gagGCATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATg       >  2:2293721/1‑93 (MQ=255)

                                                                                      cATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGt   <  2:7015146/93‑1 (MQ=255)

                                                                                      cATTCC‑GGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGt   <  1:388776/93‑1 (MQ=255)

AAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGTA  >  NC_000913/3873533‑3873713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`