Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,392,651 (G)5→4 coding (1008/1548 nt) yjeF → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,6470G.79.3% 719.5 / 207.3 270coding (1004/1548 nt)yjeFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base . (100/114);  total (126/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 294 total aligned reads displayed.

GATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATA  >  NC_000913/4392555‑4392739
                                                                                            |                                                                                            
gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTg                                                                                              <  1:5289861/93‑1 (MQ=255)
gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTg                                                                                              >  1:3424509/1‑93 (MQ=255)
gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTg                                                                                              >  1:2978164/1‑93 (MQ=255)
 aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                                                                                             >  1:5127924/1‑93 (MQ=255)
 aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                                                                                             <  2:2607918/93‑1 (MQ=255)
 aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                                                                                             <  1:876007/93‑1 (MQ=255)
  tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                                                                                            <  1:391595/93‑1 (MQ=255)
  tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                                                                                            <  1:1154800/93‑1 (MQ=255)
  tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                                                                                            >  1:585313/1‑93 (MQ=255)
     tGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggggaa                                                                                         <  1:779544/93‑3 (MQ=255)
     tGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGa                                                                                         <  2:1565061/93‑1 (MQ=255)
      gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaa                                                                                        <  2:4244318/93‑1 (MQ=255)
       cATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaa                                                                                       >  1:604887/1‑93 (MQ=255)
       cATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaa                                                                                       <  2:3854727/93‑1 (MQ=255)
         tGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaa                                                                                     <  1:2127046/93‑1 (MQ=255)
         tGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaa                                                                                     >  2:4977584/1‑93 (MQ=255)
          gAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaag                                                                                    >  1:6685401/1‑92 (MQ=255)
           aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGc                                                                                  >  1:2256764/1‑93 (MQ=255)
           aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGc                                                                                  <  1:4997672/93‑1 (MQ=255)
            aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCa                                                                                 <  1:6150055/93‑1 (MQ=255)
            aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCa                                                                                 <  1:1403417/93‑1 (MQ=255)
               gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACt                                                                               <  1:1115617/93‑1 (MQ=255)
                 cGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGc                                                                             >  1:2611355/1‑93 (MQ=255)
                  gATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaa                                                                           >  1:2160432/1‑93 (MQ=255)
                   aTGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaa                                                                          <  1:3729902/93‑1 (MQ=255)
                   aTGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaa                                                                          >  2:5352362/1‑93 (MQ=255)
                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCaaa                                                                          >  2:6847742/1‑93 (MQ=255)
                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaa                                                                         >  1:1493283/1‑93 (MQ=255)
                     ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaaa                                                                        <  2:28558/93‑1 (MQ=255)
                       aCTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGt                                                                      >  2:6987144/1‑93 (MQ=255)
                        ctctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGtt                                                                     >  1:2470022/1‑93 (MQ=255)
                         tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGtt                                                                     <  1:6085219/93‑1 (MQ=255)
                         tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTg                                                                    <  1:98497/93‑1 (MQ=255)
                           tctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTgag                                                                  <  1:690354/93‑1 (MQ=255)
                            ctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTgaga                                                                 <  1:4115101/93‑1 (MQ=255)
                             ttACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTACCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGaa                                                                >  2:3604694/1‑93 (MQ=255)
                              tACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGTTTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAt                                                               >  1:4252874/1‑93 (MQ=255)
                              tACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAt                                                               >  2:2245658/1‑93 (MQ=255)
                              tACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAt                                                               >  1:5564748/1‑93 (MQ=255)
                               aCCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtt                                                              <  2:4864126/93‑1 (MQ=255)
                               aCCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtt                                                              <  1:706258/93‑1 (MQ=255)
                               aCCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtt                                                              <  1:6513621/93‑1 (MQ=255)
                                 cGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                            <  2:3460067/93‑1 (MQ=255)
                                 cGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                            >  1:5706691/1‑93 (MQ=255)
                                 cGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                            <  1:1086649/93‑1 (MQ=255)
                                 cGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                            >  1:662234/1‑93 (MQ=255)
                                  gAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTc                                                           <  2:1641393/93‑1 (MQ=255)
                                   aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTc                                                           <  2:4411888/93‑1 (MQ=255)
                                   aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                          >  2:7498385/1‑93 (MQ=255)
                                    aaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                          >  1:7216026/1‑93 (MQ=255)
                                    aaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                          >  2:5177246/1‑93 (MQ=255)
                                     aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                        <  1:71728/93‑1 (MQ=255)
                                      gCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaa                                                       <  1:3810068/93‑1 (MQ=255)
                                       ccTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaaa                                                      >  2:4150199/1‑93 (MQ=255)
                                         tGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                    >  2:5920079/1‑93 (MQ=255)
                                         tGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                    >  2:2382290/1‑93 (MQ=255)
                                          ggAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                   >  1:7485983/1‑93 (MQ=255)
                                           gAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGa                                                  >  1:4169157/1‑93 (MQ=255)
                                            aaTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGa                                                  <  2:3998760/93‑1 (MQ=255)
                                              tGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtg                                                <  1:7358980/93‑1 (MQ=255)
                                               gggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgtt                                              >  2:5103801/1‑93 (MQ=255)
                                                 gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttgt                                            <  2:5017669/93‑1 (MQ=255)
                                                  ccGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                           <  1:3634982/93‑1 (MQ=255)
                                                   cGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                           >  2:1726614/1‑93 (MQ=255)
                                                   cGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                          <  2:1227904/93‑1 (MQ=255)
                                                   cGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                          <  2:4744743/93‑1 (MQ=255)
                                                    gATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                         >  1:3761821/1‑93 (MQ=255)
                                                    gATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                         >  2:7070870/1‑93 (MQ=255)
                                                    gATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                         >  1:1856965/1‑93 (MQ=255)
                                                    gATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTggg                                         >  1:4352704/1‑93 (MQ=255)
                                                     aTGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                        >  1:2028303/1‑93 (MQ=255)
                                                     aTGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                        <  2:5643851/93‑1 (MQ=255)
                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGTGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                        <  2:1446729/93‑1 (MQ=255)
                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                        >  2:5462898/1‑93 (MQ=255)
                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGAt                                       >  2:7419211/1‑93 (MQ=255)
                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGAt                                       >  2:2092765/1‑93 (MQ=255)
                                                           tggtgATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                                   >  1:4893622/1‑93 (MQ=255)
                                                            ggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAt                                 >  1:3117449/1‑93 (MQ=255)
                                                                 ttGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCAtt                            <  1:6343720/93‑1 (MQ=255)
                                                                  tGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTg                           <  2:3175400/93‑1 (MQ=255)
                                                                     tCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAAc                        <  1:3524138/93‑1 (MQ=255)
                                                                       ccGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGCTGCCGATGCATTGACCct                      >  1:5571448/1‑93 (MQ=255)
                                                                         ggTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc                    >  2:2350899/1‑93 (MQ=255)
                                                                          gTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc                    <  2:2928272/93‑1 (MQ=255)
                                                                           tCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg                  >  1:1613605/1‑93 (MQ=255)
                                                                            cTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg                  <  2:84353/93‑1 (MQ=255)
                                                                             tGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGc                >  2:6784358/1‑93 (MQ=255)
                                                                              gggCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGca               >  2:4079937/1‑93 (MQ=255)
                                                                                 ccAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat             <  2:4592139/93‑1 (MQ=255)
                                                                                  cAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatca           >  1:4874720/1‑93 (MQ=255)
                                                                                   aGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcna          >  2:7217291/1‑93 (MQ=255)
                                                                                   aGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaa          <  2:2489420/93‑1 (MQ=255)
                                                                                     cAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaat         >  2:5984689/1‑93 (MQ=255)
                                                                                     cAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCaatcaatc        >  1:4948331/1‑93 (MQ=255)
                                                                                     cAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCaatcaatc        <  1:5423625/93‑1 (MQ=255)
                                                                                      aaGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCATTCAATcc       <  2:5775461/93‑1 (MQ=255)
                                                                                      aaGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATcc       >  1:4014544/1‑93 (MQ=255)
                                                                                       agagT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATccc      <  2:6343055/93‑1 (MQ=255)
                                                                                          gTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGa    >  2:5608548/1‑93 (MQ=255)
                                                                                            gggggAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATa  <  1:6467180/93‑1 (MQ=255)
                                                                                            |                                                                                            
GATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATA  >  NC_000913/4392555‑4392739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑