BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,415,570	(T)_8→9	coding (218/276 nt)	racC ←	defective prophage rac

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,415,562	1	.	T	78.7%	685.5 / 175.6	225	coding (226/276 nt)	racC	defective prophage rac
Reads supporting (aligned to +/- strand): ref base . (25/23); new base T (92/85); total (117/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

TACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACC  >  NC_000913/1415470‑1415652

 acacacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑t                                                                                           >  1:7390056/1‑93 (MQ=255)

  cacacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tt                                                                                          <  1:842568/93‑1 (MQ=255)

  cacacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tt                                                                                          >  1:3327294/1‑93 (MQ=255)

   acacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑ttt                                                                                         <  2:2988561/93‑1 (MQ=255)

   acacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑ttt                                                                                         >  2:2536090/1‑93 (MQ=255)

    cacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tttt                                                                                        <  2:5763763/93‑1 (MQ=255)

    cacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tttt                                                                                        <  2:123552/93‑1 (MQ=255)

      cTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tttttt                                                                                      <  2:3223948/93‑1 (MQ=255)

      cTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tttttt                                                                                      >  2:4576272/1‑93 (MQ=255)

        gTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tttttttt                                                                                    >  2:6410172/1‑93 (MQ=255)

         ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑ttttttttt                                                                                   <  2:5477048/93‑2 (MQ=255)

          tCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tttttttttc                                                                                  <  2:5891374/93‑3 (MQ=255)

          tCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCt                                                                                  <  2:5435626/93‑1 (MQ=255)

           cATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑tttttttttct                                                                                 <  1:7465889/93‑4 (MQ=255)

            aTAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGc                                                                                >  1:2428859/1‑93 (MQ=255)

               gCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCgg                                                                              <  2:7181507/93‑1 (MQ=255)

                ccTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCggg                                                                             <  1:2700074/93‑1 (MQ=255)

                ccTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCggg                                                                             >  2:5680697/1‑93 (MQ=255)

                 cTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGc                                                                            >  2:269055/1‑93 (MQ=255)

                 cTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGc                                                                            >  2:5111473/1‑93 (MQ=255)

                 cTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGc                                                                            >  1:6803532/1‑93 (MQ=255)

                  tGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCa                                                                           >  1:3216257/1‑93 (MQ=255)

                    cgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGa                                                                         <  2:301751/93‑1 (MQ=255)

                    cgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGa                                                                         <  2:1090230/93‑1 (MQ=255)

                    cgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGAt                                                                        <  1:6743539/93‑1 (MQ=255)

                     gcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGAt                                                                        >  2:127944/1‑93 (MQ=255)

                       cATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTc                                                                      <  1:4258941/93‑1 (MQ=255)

                        aTACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCt                                                                     >  1:2288369/1‑93 (MQ=255)

                         tACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCtt                                                                    >  2:4436472/1‑93 (MQ=255)

                         tACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCtt                                                                    <  2:3320774/93‑1 (MQ=255)

                         tACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCtt                                                                    <  2:658058/93‑1 (MQ=255)

                           cGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTCTTTCTGCGGGCAGATTCTTTcc                                                                 <  1:619731/93‑1 (MQ=255)

                            gCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTcc                                                                 >  1:7043272/1‑93 (MQ=255)

                            gCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCg                                                                >  1:1456519/1‑93 (MQ=255)

                             cAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGa                                                               >  1:4570858/1‑93 (MQ=255)

                              aGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGa                                                               <  1:7266418/93‑1 (MQ=255)

                                gCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGata                                                             <  1:5322430/93‑1 (MQ=255)

                                gCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGata                                                             <  2:6330333/93‑1 (MQ=255)

                                 cTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATg                                                           >  2:842307/1‑93 (MQ=255)

                                  tATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATg                                                           <  1:2902285/93‑1 (MQ=255)

                                   aTTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGc                                                          >  2:1468315/1‑93 (MQ=255)

                                   aTTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGc                                                          <  2:1385134/93‑1 (MQ=255)

                                   aTTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGAGTCTTTCCGATATGCa                                                         <  1:6341867/93‑1 (MQ=255)

                                     ttctttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCAc                                                        >  1:2365096/1‑93 (MQ=255)

                                      tctttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCa                                                      >  1:26193/1‑93 (MQ=255)

                                      tctttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCa                                                      >  1:1398495/1‑93 (MQ=255)

                                        tttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAg                                                     <  1:3188667/93‑1 (MQ=255)

                                        tttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAg                                                     >  2:4667814/1‑93 (MQ=255)

                                         ttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAgg                                                    >  1:2027285/1‑93 (MQ=255)

                                             gATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGcaca                                                >  1:4726029/1‑93 (MQ=255)

                                             gATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGcaca                                                >  2:2775877/1‑93 (MQ=255)

                                               tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtt                                              <  1:688056/93‑1 (MQ=255)

                                               tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtt                                              <  2:4793687/93‑1 (MQ=255)

                                               tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtt                                              <  2:7149678/93‑1 (MQ=255)

                                               tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtt                                              <  2:1569874/93‑1 (MQ=255)

                                               tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtt                                              <  2:735624/93‑1 (MQ=255)

                                               tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtt                                              <  2:2165341/93‑1 (MQ=255)

                                               tttCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttt                                             >  2:6690612/1‑93 (MQ=255)

                                                  cACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGTT‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttt                                           <  2:1638897/93‑1 (MQ=255)

                                                   aCCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtttttt                                          >  1:1653030/1‑93 (MQ=255)

                                                   aCCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtttttt                                          >  1:665261/1‑93 (MQ=255)

                                                   aCCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttttt                                         <  1:5441676/93‑1 (MQ=255)

                                                   aCCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttttt                                         >  1:5926741/1‑93 (MQ=255)

                                                    ccTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttttt                                         <  1:5487244/93‑1 (MQ=255)

                                                    ccTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttttt                                         >  2:2063290/1‑93 (MQ=255)

                                                     cTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTg                                        <  2:1769994/93‑1 (MQ=255)

                                                      ttttAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGa                                       >  2:4229054/1‑93 (MQ=255)

                                                       tttAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGac                                      >  2:1932020/1‑93 (MQ=255)

                                                       tttAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGac                                      >  2:3464555/1‑93 (MQ=255)

                                                          aaTTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACAcc                                   >  2:5430453/1‑93 (MQ=255)

                                                           aTTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCt                                  <  1:3817039/93‑1 (MQ=255)

                                                            tttCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCtt                                 >  1:985458/1‑93 (MQ=255)

                                                            tttCATTGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCtt                                 >  1:6768200/1‑93 (MQ=255)

                                                                 ttGCAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtcatca                            >  2:3857737/1‑93 (MQ=255)

                                                                   gCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgt                         <  1:6169269/93‑1 (MQ=255)

                                                                    cAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgt                         <  1:4934679/93‑1 (MQ=255)

                                                                    cAATTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgt                         <  1:3769262/93‑1 (MQ=255)

                                                                    cAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgtg                        <  2:6812915/93‑1 (MQ=255)

                                                                     aaTTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgtg                        >  1:4820607/1‑93 (MQ=255)

                                                                     aaTTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgtg                        >  2:2274221/1‑93 (MQ=255)

                                                                     aaTTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgtgt                       >  2:7030348/1‑93 (MQ=255)

                                                                     aaTTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgtgt                       >  1:4125533/1‑93 (MQ=255)

                                                                      aTTAGAGTTGCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgtgt                       <  1:4082877/93‑1 (MQ=255)

                                                                             ttGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGt               <  1:1037204/93‑1 (MQ=255)

                                                                               gCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGtt              >  1:2534338/1‑93 (MQ=255)

                                                                               gCCAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGtt              <  1:5760167/93‑1 (MQ=255)

                                                                                ccAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTg             >  2:3064613/1‑93 (MQ=255)

                                                                                 cAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGc            <  2:4842896/93‑1 (MQ=255)

                                                                                 cAGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGc            >  2:7468031/1‑93 (MQ=255)

                                                                                 cAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGcc           >  2:1520534/1‑93 (MQ=255)

                                                                                  aGAAATTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGcc           >  2:40723/1‑93 (MQ=255)

                                                                                     aaTTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGa        >  2:7306468/1‑93 (MQ=255)

                                                                                      aTTCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGAt       <  1:5673654/93‑1 (MQ=255)

                                                                                       ttCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATg      >  1:7153089/1‑93 (MQ=255)

                                                                                       ttCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATg      >  1:5007171/1‑93 (MQ=255)

                                                                                       ttCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATg      >  2:1659958/1‑93 (MQ=255)

                                                                                        tCGG‑CTTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATgg     <  2:505743/93‑1 (MQ=255)

                                                                                        tCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGa    <  2:6602885/93‑1 (MQ=255)

                                                                                         cGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAc   <  1:1162762/93‑1 (MQ=255)

                                                                                          gg‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAcc  >  2:4177083/1‑93 (MQ=255)

TACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGG‑C‑TTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACC  >  NC_000913/1415470‑1415652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`