Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,415,996 (T)6→7 coding (37/171 nt) ydaE ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,415,9901.T80.4% 783.1 / 180.7 250coding (43/171 nt)ydaEhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (26/23);  new base T (97/104);  total (123/127)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 287 total aligned reads displayed.

AGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTT  >  NC_000913/1415898‑1416081
                                                                                             |                                                                                           
 gCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑t                                                                                            >  2:3697666/1‑93 (MQ=255)
 gCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑t                                                                                            >  2:2071088/1‑93 (MQ=255)
   ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttt                                                                                          <  2:1192595/93‑1 (MQ=255)
   ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttt                                                                                          <  1:815495/93‑1 (MQ=255)
    tCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttt                                                                                         <  1:7165077/93‑1 (MQ=255)
     cACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttt                                                                                        <  1:4066829/93‑1 (MQ=255)
       cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttttt                                                                                      >  2:6349805/1‑92 (MQ=255)
       cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttttt                                                                                      <  1:1328903/93‑2 (MQ=255)
       cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttttt                                                                                      <  1:4763100/93‑2 (MQ=255)
       cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑ttttttt                                                                                      <  2:1517724/93‑2 (MQ=255)
        tGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttttg                                                                                     >  1:945350/1‑91 (MQ=255)
        tGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttttg                                                                                     <  1:3394371/93‑3 (MQ=255)
         gCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑tttttttgc                                                                                    >  1:5066289/1‑90 (MQ=255)
            atatTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCaaa                                                                                  <  2:3528315/93‑1 (MQ=255)
               ttttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggt                                                                               >  2:1603467/1‑93 (MQ=255)
               ttttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggt                                                                               <  2:4809853/93‑1 (MQ=255)
                tttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtg                                                                              <  1:4167267/93‑1 (MQ=255)
                 ttCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtgg                                                                             >  2:7096311/1‑93 (MQ=255)
                  tctcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtggt                                                                            <  2:5366451/93‑1 (MQ=255)
                  tctcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAggtggt                                                                            <  2:6181332/93‑1 (MQ=255)
                   ctcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTa                                                                           <  1:3297795/93‑1 (MQ=255)
                   ctcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTaa                                                                          <  2:6707637/93‑1 (MQ=255)
                    tcGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTaa                                                                          >  1:1038997/1‑93 (MQ=255)
                       gTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGcac                                                                      >  2:6879471/1‑93 (MQ=255)
                        ttCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGcac                                                                      >  2:5208361/1‑93 (MQ=255)
                        ttCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGcac                                                                      >  1:3523079/1‑93 (MQ=255)
                         tCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGcaca                                                                     <  2:3795028/93‑1 (MQ=255)
                         tCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGcaca                                                                     <  2:5907811/93‑1 (MQ=255)
                         tCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACat                                                                    <  2:3240975/93‑1 (MQ=255)
                           gTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACatt                                                                   >  1:6129662/1‑93 (MQ=255)
                             cTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACatttt                                                                 <  2:303999/93‑1 (MQ=255)
                             cTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACatttt                                                                 <  1:5230083/93‑1 (MQ=255)
                             cTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACatttta                                                                >  1:4337313/1‑93 (MQ=255)
                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACatttta                                                                <  2:1107576/93‑1 (MQ=255)
                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACatttta                                                                <  1:6441296/93‑1 (MQ=255)
                              ttttGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACatttta                                                                <  1:5758451/93‑1 (MQ=255)
                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTc                                                           >  2:2606681/1‑93 (MQ=255)
                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTc                                                           <  2:2786842/93‑1 (MQ=255)
                                   aTAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTc                                                           >  2:6835218/1‑93 (MQ=255)
                                     aaaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCtt                                                         <  1:7306468/93‑1 (MQ=255)
                                     aaaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCtt                                                         <  2:4481754/93‑1 (MQ=255)
                                      aaaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTa                                                        <  1:4850686/93‑1 (MQ=255)
                                       aaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAg                                                       <  2:3332006/93‑1 (MQ=255)
                                       aaTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAg                                                       >  2:4127959/1‑93 (MQ=255)
                                        aTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGt                                                      <  2:6023523/93‑1 (MQ=255)
                                        aTCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGt                                                      >  2:4973081/1‑93 (MQ=255)
                                              tGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCAc                                                <  1:2518247/93‑1 (MQ=255)
                                               gCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCa                                              >  1:2034760/1‑93 (MQ=255)
                                                cATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCa                                              <  1:5766740/93‑1 (MQ=255)
                                                cATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCa                                              <  2:5088569/93‑1 (MQ=255)
                                                cATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCa                                              <  2:2546488/93‑1 (MQ=255)
                                                   gaagtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGa                                           <  1:987753/93‑1 (MQ=255)
                                                     agtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACt                                         >  2:6548067/1‑93 (MQ=255)
                                                      gtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTc                                        <  1:6010913/93‑1 (MQ=255)
                                                      gtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTc                                        >  1:6553920/1‑93 (MQ=255)
                                                      gtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTc                                        <  1:6238156/93‑1 (MQ=255)
                                                      gtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTc                                        <  2:4841772/93‑1 (MQ=255)
                                                      gtgaagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTcc                                       >  1:3981831/1‑93 (MQ=255)
                                                         aagCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGc                                     <  1:1755754/93‑1 (MQ=255)
                                                          agCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGcc                                    <  1:305233/93‑1 (MQ=255)
                                                           gCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCt                                   <  1:3848824/93‑1 (MQ=255)
                                                           gCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCt                                   >  1:3197856/1‑93 (MQ=255)
                                                            cATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTACTCATCACCATGACTCCGCCtt                                  >  1:2466866/1‑93 (MQ=255)
                                                             aTTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCttt                                 <  2:3414310/93‑1 (MQ=255)
                                                              ttCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTa                                >  2:5702588/1‑93 (MQ=255)
                                                                cTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACa                              >  2:1864354/1‑93 (MQ=255)
                                                                cTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCACGACTCCGCCTTTACa                              <  1:5385456/93‑1 (MQ=255)
                                                                cTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAg                             <  1:15319/93‑1 (MQ=255)
                                                                 tttCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAg                             >  1:1898150/1‑93 (MQ=255)
                                                                 tttCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAg                             >  1:2836467/1‑93 (MQ=255)
                                                                 tttCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAgg                            >  1:5503614/1‑93 (MQ=255)
                                                                  ttCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAgg                            >  2:5588942/1‑93 (MQ=255)
                                                                  ttCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGt                           >  2:2378188/1‑93 (MQ=255)
                                                                   tCAATAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGt                           >  1:4614506/1‑93 (MQ=255)
                                                                     aaTAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTaa                         >  1:2647325/1‑93 (MQ=255)
                                                                     aaTAGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTaa                         >  1:3221429/1‑93 (MQ=255)
                                                                     aaTAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTaaa                        <  2:784021/93‑1 (MQ=255)
                                                                        aGCTTTGCTTTCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAAcc                      >  2:5802391/1‑93 (MQ=255)
                                                                         gctttgctttCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCAt                    >  2:4366702/1‑93 (MQ=255)
                                                                           tttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATc                   <  1:1069981/93‑1 (MQ=255)
                                                                           tttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATc                   >  1:2287611/1‑93 (MQ=255)
                                                                           tttgctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATc                   >  1:5326534/1‑93 (MQ=255)
                                                                           tttgctttCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCa                  >  2:7348466/1‑93 (MQ=255)
                                                                           tttgctttCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCa                  <  2:5745920/93‑1 (MQ=255)
                                                                              gctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACg                >  1:3908234/1‑93 (MQ=255)
                                                                               ctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGa               <  1:1184535/93‑1 (MQ=255)
                                                                               ctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGa               <  2:1691932/93‑1 (MQ=255)
                                                                               ctttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGa               <  2:6082884/93‑1 (MQ=255)
                                                                               ctttCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAc              <  2:3992938/93‑1 (MQ=255)
                                                                                tttCTTCAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGAc              <  1:2403990/93‑1 (MQ=255)
                                                                                  tcttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCg            >  1:2175295/1‑93 (MQ=255)
                                                                                  tcttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCg            >  1:4123457/1‑93 (MQ=255)
                                                                                  tcttcAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGa           >  2:6228304/1‑93 (MQ=255)
                                                                                   cttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGa           <  2:474366/93‑1 (MQ=255)
                                                                                    ttcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAg          >  2:5440196/1‑93 (MQ=255)
                                                                                    ttcAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAgg         <  1:456564/93‑1 (MQ=255)
                                                                                     tcAACGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAgg         <  1:7465759/93‑1 (MQ=255)
                                                                                       aaCGTCTTTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGaa       >  1:3620072/1‑93 (MQ=255)
                                                                                          gTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACt   <  1:7078646/93‑1 (MQ=255)
                                                                                           tC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACtt  >  1:6823475/1‑93 (MQ=255)
                                                                                             |                                                                                           
AGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTC‑TTTTTTGCAAAGGTGGTAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTT  >  NC_000913/1415898‑1416081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑