BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,037,676	(C)_5→6	coding (177/1005 nt)	b1971 →	putative reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,037,671	1	.	C	84.0%	527.4 / 96.1	189	coding (172/1005 nt)	b1971	putative reductase
Reads supporting (aligned to +/- strand): ref base . (15/15); new base C (83/75); total (99/90)
Fisher's exact test for biased strand distribution p-value = 8.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 221 total aligned reads displayed.

AAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATA  >  NC_000913/2037579‑2037761

 aGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgcccg‑cccg‑c                                                                                            <  2:3976099/93‑1 (MQ=255)

  gCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgcccg‑cccg‑cc                                                                                           >  1:5926473/1‑93 (MQ=255)

  gCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgcccg‑cccg‑cc                                                                                           <  1:4201443/93‑1 (MQ=255)

    aCTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑cccg‑cccc                                                                                         >  1:6703314/1‑93 (MQ=255)

    aCTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑cccg‑cccc                                                                                         <  1:4819350/93‑1 (MQ=255)

         gCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑cccg‑ccccccgcc                                                                                    <  2:3146065/93‑5 (MQ=255)

         gCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCccgcc‑g                                                                                   >  1:3461537/1‑93 (MQ=255)

            tCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGaa                                                                                >  1:6357450/1‑93 (MQ=255)

            tCAGTGCAACTGCACTTTCTTTGCCTCACGCCGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGa                                                                                 >  2:1511407/1‑93 (MQ=255)

               gTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGaaaa                                                                              <  1:1596221/93‑1 (MQ=255)

               gTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGaaaa                                                                              >  2:1201046/1‑93 (MQ=255)

               gTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACCATCGCCCG‑CCCGCCCCCCGCC‑GGaaaa                                                                              <  2:6429258/93‑1 (MQ=255)

                tGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAg                                                                             <  2:4091016/93‑1 (MQ=255)

                tGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAg                                                                             >  2:6104102/1‑93 (MQ=255)

                    aCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCt                                                                         >  2:3635273/1‑93 (MQ=255)

                    aCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCt                                                                         >  1:5065993/1‑93 (MQ=255)

                    aCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTg                                                                        <  2:2850104/93‑1 (MQ=255)

                     cTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTg                                                                        <  1:2294785/93‑1 (MQ=255)

                      tGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTgg                                                                       <  2:1702253/93‑1 (MQ=255)

                      tGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTgg                                                                       <  1:3214730/93‑1 (MQ=255)

                       gCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGa                                                                      >  1:6537310/1‑93 (MQ=255)

                       gCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGa                                                                      >  2:3106190/1‑93 (MQ=255)

                       gCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGa                                                                      >  1:4058992/1‑93 (MQ=255)

                         aCTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGt                                                                    >  1:1901960/1‑93 (MQ=255)

                         aCTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGt                                                                    >  2:2572113/1‑93 (MQ=255)

                         aCTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGt                                                                    <  2:2519246/93‑1 (MQ=255)

                          ctttctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGtt                                                                   >  2:6157244/1‑93 (MQ=255)

                           tttctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAATGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTc                                                                  <  2:7239969/93‑1 (MQ=255)

                           tttctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTc                                                                  >  2:2416621/1‑93 (MQ=255)

                           tttctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTc                                                                  <  2:6554881/93‑1 (MQ=255)

                             tctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAg                                                                >  1:2495431/1‑93 (MQ=255)

                             tctttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAg                                                                >  2:1172704/1‑93 (MQ=255)

                               tttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCa                                                              <  2:4301789/93‑1 (MQ=255)

                               tttGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCaa                                                             <  1:259715/93‑1 (MQ=255)

                                  gCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGc                                                           >  1:937865/1‑93 (MQ=255)

                                    cTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCt                                                         <  2:5077964/93‑1 (MQ=255)

                                    cTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCt                                                         >  2:6361931/1‑93 (MQ=255)

                                      cACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTgc                                                       >  2:1750240/1‑93 (MQ=255)

                                      cACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTgc                                                       <  2:4172622/93‑1 (MQ=255)

                                      cACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTgc                                                       <  1:7138424/93‑1 (MQ=255)

                                       aCGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTgcc                                                      >  2:7090288/1‑93 (MQ=255)

                                        cGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTgccgc                                                    >  2:4447460/1‑93 (MQ=255)

                                            gcgcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTg                                                 >  1:5295982/1‑93 (MQ=255)

                                            gcgcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCgg                                                >  1:2573443/1‑93 (MQ=255)

                                              gcATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGc                                               >  1:1107395/1‑93 (MQ=255)

                                                aTGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaa                                             >  2:776298/1‑93 (MQ=255)

                                                aTGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaa                                             >  1:523060/1‑93 (MQ=255)

                                                 tGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaaa                                            >  1:2151184/1‑93 (MQ=255)

                                                 tGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCaaaa                                           >  1:1297117/1‑93 (MQ=255)

                                                  gCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCaaaa                                           <  2:5662327/93‑1 (MQ=255)

                                                    cGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATa                                         >  2:4596391/1‑93 (MQ=255)

                                                    cGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATa                                         <  1:551345/93‑1 (MQ=255)

                                                     gATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATaa                                        >  1:3224928/1‑93 (MQ=255)

                                                     gATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATaa                                        >  2:5382676/1‑93 (MQ=255)

                                                     gATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATaa                                        >  1:5989625/1‑93 (MQ=255)

                                                      ntCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATANc                                       >  2:5813182/2‑93 (MQ=255)

                                                      aTCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAc                                       >  1:5698033/1‑93 (MQ=255)

                                                       tCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAcc                                      <  1:1024647/93‑1 (MQ=255)

                                                       tCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAcc                                      <  1:3869225/93‑1 (MQ=255)

                                                       tCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAAcc                                      <  2:6220857/93‑1 (MQ=255)

                                                         tGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTg                                    >  1:5776929/1‑93 (MQ=255)

                                                         tGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTg                                    >  1:2098399/1‑93 (MQ=255)

                                                          gCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGc                                   >  1:686926/1‑93 (MQ=255)

                                                          gCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGc                                   >  1:1872718/1‑93 (MQ=255)

                                                          gCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGc                                   <  1:1722151/93‑1 (MQ=255)

                                                           cTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGcc                                  >  2:5595653/1‑93 (MQ=255)

                                                           cTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGcc                                  <  2:5326135/93‑1 (MQ=255)

                                                            ttAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCAc                                <  2:231363/93‑1 (MQ=255)

                                                            ttAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCAc                                <  2:1068461/93‑1 (MQ=255)

                                                              aGCTGGTTTAAAGGGAACGAGCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACt                               <  1:4664719/93‑1 (MQ=255)

                                                                cTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGa                             <  2:4393903/93‑1 (MQ=255)

                                                                cTGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGa                             <  1:3615513/93‑1 (MQ=255)

                                                                 tGGTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGAc                            >  2:6774529/1‑93 (MQ=255)

                                                                   gTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGc                          >  1:7229008/1‑93 (MQ=255)

                                                                   gTTTAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGc                          >  1:2664064/1‑93 (MQ=255)

                                                                    tttAAAGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGcc                         <  2:2992847/93‑1 (MQ=255)

                                                                       aaaGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCACTGACGCcagca                     >  2:1037274/1‑93 (MQ=255)

                                                                       aaaGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCACTGACGCcagca                     <  2:2017582/93‑1 (MQ=255)

                                                                        aaGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCcagca                     >  2:476906/1‑93 (MQ=255)

                                                                         aGGGACCGACCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCACTGACGCCAGCAGa                   <  1:5464290/93‑1 (MQ=255)

                                                                         aGGGAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCcagcag                    <  1:6694498/93‑1 (MQ=255)

                                                                          gggACCGACCCCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGa                   <  1:719062/93‑1 (MQ=255)

                                                                          gggAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGa                   <  2:7403514/93‑1 (MQ=255)

                                                                          gggAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGa                   >  2:2708606/1‑93 (MQ=255)

                                                                            gAACGATCGCCCGCCCCC‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATa                 <  1:310024/93‑1 (MQ=255)

                                                                            gAACGATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATa                 <  2:3854350/93‑1 (MQ=255)

                                                                                gATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGt             <  2:5171059/93‑1 (MQ=255)

                                                                                gATCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGt             <  2:651937/93‑1 (MQ=255)

                                                                                 aTCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGtt            >  2:6284692/1‑93 (MQ=255)

                                                                                  tCGCCCG‑CCCGCCCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGttt           <  2:4895468/93‑1 (MQ=255)

                                                                                    gcccg‑cccgcccCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTcc         <  1:277448/93‑1 (MQ=255)

                                                                                    ccccc‑cccc‑ccccCGCCGGGAAAAGCGCGGGATTTCAGCAAGCCTGCCGCCTGGAAAAAAACCCTGCCACTGACGCCAGCAGATAAAGTTTcc         <  1:6179316/92‑1 (MQ=255)

                                                                                     cccg‑cccgcccCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCg        >  2:5233758/1‑93 (MQ=255)

                                                                                     cccg‑cccgcccCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCg        <  2:1659745/93‑1 (MQ=255)

                                                                                     cccg‑cccgcccCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCg        >  1:6816858/1‑93 (MQ=255)

                                                                                     cccc‑cccg‑cccCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCgg       <  1:4455221/93‑1 (MQ=255)

                                                                                      ccg‑cccgcccCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCgg       >  1:7101971/1‑93 (MQ=255)

                                                                                      ccg‑cccg‑cccCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGt      <  2:5715039/93‑1 (MQ=255)

                                                                                       cg‑cccgcccCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGt      <  1:3397169/93‑1 (MQ=255)

                                                                                       cg‑cccg‑cccCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCCGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGtt     >  1:7292654/1‑93 (MQ=255)

AAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG‑CCCG‑CCCCCGCC‑GGAAAAGCGCTGGAGTTCAGCAAGCCTGCCGCCTGGCAAAATAACCTGCCACTGACGCCAGCAGATAAAGTTTCCGGTTATA  >  NC_000913/2037579‑2037761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`