Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,184,686 (C)5→6 coding (750/825 nt) yohM → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,184,6811.C78.5% 571.0 / 162.2 215coding (745/825 nt)yohMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (21/25);  new base C (94/74);  total (116/99)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-01

Only 100 of 246 total aligned reads displayed.

CGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCT  >  NC_000913/2184589‑2184770
                                                                                             |                                                                                          
cGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAAcgcg                                                                                             <  1:5437335/93‑1 (MQ=255)
 gTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACgcg‑‑c                                                                                          <  2:5747207/93‑1 (MQ=255)
  ttAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑cc                                                                                         <  2:7205505/93‑1 (MQ=255)
    aaCGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑cccc                                                                                       <  2:2728459/93‑1 (MQ=255)
     aCGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑ccccc                                                                                      <  2:6282858/93‑1 (MQ=255)
     aCGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑ccccc                                                                                      >  2:2252807/1‑93 (MQ=255)
       gCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑cccccct                                                                                    <  2:1867489/93‑3 (MQ=255)
       gCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑cccccct                                                                                    >  2:3087305/1‑91 (MQ=255)
       gCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑cccccct                                                                                    >  1:1013694/1‑91 (MQ=255)
       gCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTa                                                                                    >  1:5920599/1‑93 (MQ=255)
         ttGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑cccccctat                                                                                  <  2:7275330/93‑5 (MQ=255)
               ccGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTcc                                                                             >  1:7468043/1‑93 (MQ=255)
               ccGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCa                                                                            <  1:5207681/93‑1 (MQ=255)
                cGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCa                                                                            >  2:4686837/1‑93 (MQ=255)
                  tAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGt                                                                          >  1:2966414/1‑93 (MQ=255)
                  tAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGt                                                                          <  1:6872173/93‑1 (MQ=255)
                   aGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTc                                                                         <  1:4559303/93‑1 (MQ=255)
                    ggCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCt                                                                        <  2:7058567/93‑1 (MQ=255)
                    ggCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTg                                                                       >  2:3898645/1‑93 (MQ=255)
                     gCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGt                                                                      <  2:1021931/93‑1 (MQ=255)
                     gCGTTGGCACAGAAATCAGAGTTCAGCAGGACAGACAGCCCTGGAACGGATTAAACGCTCTCCCTAACCGCC‑‑CCCCCTATTTTTCCAGTCTGt                                                                      <  1:691715/93‑1 (MQ=255)
                      cGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTTGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGt                                                                      <  1:6684304/93‑1 (MQ=255)
                        ttGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTg                                                                    >  2:2020185/1‑93 (MQ=255)
                        ttGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTg                                                                    >  2:3342186/1‑93 (MQ=255)
                        ttGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGa                                                                   <  1:1538004/93‑1 (MQ=255)
                         tGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGa                                                                   <  1:6076230/93‑1 (MQ=255)
                          ggCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACCCTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGAt                                                                  <  1:5863045/93‑1 (MQ=255)
                               agcaATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCt                                                             <  2:3567184/93‑1 (MQ=255)
                               agcaATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCt                                                             <  2:7184167/93‑1 (MQ=255)
                                gcaATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCtt                                                            >  2:1647992/1‑93 (MQ=255)
                                gcaATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCtt                                                            >  1:61257/1‑93 (MQ=255)
                                gcaATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCtt                                                            >  2:2226972/1‑93 (MQ=255)
                                 caATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTa                                                           >  1:4690033/1‑93 (MQ=255)
                                  aaTCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAg                                                          <  1:1603060/93‑1 (MQ=255)
                                  aaTCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAg                                                          >  1:3416429/1‑93 (MQ=255)
                                   aTCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                         <  2:879933/93‑1 (MQ=255)
                                   aTCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                         >  2:6679899/1‑93 (MQ=255)
                                      aGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCgg                                                      <  2:2900286/93‑1 (MQ=255)
                                       gCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                     >  2:498335/1‑93 (MQ=255)
                                       gCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                     <  2:749429/93‑1 (MQ=255)
                                       gCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                     >  2:2473088/1‑93 (MQ=255)
                                         gTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgtgtg                                                  >  1:547371/1‑93 (MQ=255)
                                          ttCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgtgtg                                                  >  1:6400393/1‑93 (MQ=255)
                                          ttCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgtgtgt                                                 >  1:2743785/1‑93 (MQ=255)
                                              gcagGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGtata                                              <  1:1922391/93‑1 (MQ=255)
                                              gcagGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGtata                                              >  1:6513674/1‑93 (MQ=255)
                                                agGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATgg                                           <  2:1324015/93‑1 (MQ=255)
                                                 ggTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATg                                            >  2:5336178/1‑93 (MQ=255)
                                                 ggTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATgg                                           >  1:5379454/1‑93 (MQ=255)
                                                 ggTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATggg                                          >  1:1924961/1‑93 (MQ=255)
                                                  gTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATggg                                          >  2:2130922/1‑93 (MQ=255)
                                                     gCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGt                                       <  2:5176542/93‑1 (MQ=255)
                                                     gCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTa                                      >  2:5020394/1‑93 (MQ=255)
                                                      cAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTa                                      >  1:697383/1‑93 (MQ=255)
                                                       aaaaCGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACa                                    >  1:2022223/1‑93 (MQ=255)
                                                        aaaCGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACa                                    <  1:89673/93‑1 (MQ=255)
                                                        aaaCGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACa                                    <  2:5980143/93‑1 (MQ=255)
                                                        aaaCGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACa                                    <  2:3183590/93‑1 (MQ=255)
                                                        aaaCGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACa                                    >  1:6191731/1‑93 (MQ=255)
                                                        aaaCGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACAt                                   >  2:2331876/1‑93 (MQ=255)
                                                         aaCGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACAt                                   <  1:3077169/93‑1 (MQ=255)
                                                          aCGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATg                                  >  1:374309/1‑93 (MQ=255)
                                                           cGCTGGAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATgg                                 >  1:1440533/1‑93 (MQ=255)
                                                             cTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCtt                              >  1:2827076/1‑93 (MQ=255)
                                                               ggAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTc                             >  2:552840/1‑93 (MQ=255)
                                                                gAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCa                            <  2:6114841/93‑1 (MQ=255)
                                                                gAGCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCa                            >  2:6018885/1‑93 (MQ=255)
                                                                  gCGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATg                          <  1:4187580/93‑1 (MQ=255)
                                                                  gCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATgg                         <  1:1197401/93‑1 (MQ=255)
                                                                   cGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATgg                         >  2:6638162/1‑93 (MQ=255)
                                                                   cGGATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTACAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATgg                         >  1:7312568/1‑93 (MQ=255)
                                                                    ggATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATggg                        >  1:5680037/1‑93 (MQ=255)
                                                                    ggATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATggg                        >  2:1936324/1‑93 (MQ=255)
                                                                     gATTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGc                       >  1:48848/1‑93 (MQ=255)
                                                                      aTTTAACGCTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCa                      <  2:1469347/93‑1 (MQ=255)
                                                                      aTTTAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCa                      >  2:4345824/1‑93 (MQ=255)
                                                                        ttAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATa                    >  1:5994416/1‑93 (MQ=255)
                                                                        ttAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATaa                   >  1:4326414/1‑93 (MQ=255)
                                                                         tAACACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATaa                   <  1:6373400/93‑1 (MQ=255)
                                                                          aaCACTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAAt                  >  1:473493/1‑93 (MQ=255)
                                                                           acacTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATg                 >  1:7191817/1‑93 (MQ=255)
                                                                            cacTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGc                >  1:7513785/1‑93 (MQ=255)
                                                                             acTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATgtg               >  1:5553297/1‑91 (MQ=255)
                                                                             acTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCg               >  1:2933436/1‑93 (MQ=255)
                                                                             acTCTCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCg               >  2:2554131/1‑93 (MQ=255)
                                                                             acTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGa              >  2:4246549/1‑93 (MQ=255)
                                                                             acTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGa              >  1:3871518/1‑93 (MQ=255)
                                                                              ctctCGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGa              <  1:6264941/93‑1 (MQ=255)
                                                                               tctcGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGAt             <  2:1596181/93‑1 (MQ=255)
                                                                               tctcGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGAt             <  1:2101361/93‑1 (MQ=255)
                                                                               tctcGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGAt             <  2:4043240/93‑1 (MQ=255)
                                                                               tctcGCTAAACGCGA‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGAt             >  2:6438050/1‑93 (MQ=255)
                                                                                  cGCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAAc          >  2:3754601/1‑93 (MQ=255)
                                                                                   gCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACt         >  2:4710991/1‑93 (MQ=255)
                                                                                   gCTAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACt         >  2:1196878/1‑93 (MQ=255)
                                                                                    cTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCa       >  2:451765/1‑93 (MQ=255)
                                                                                     tAAACGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCa       >  1:3942168/1‑93 (MQ=255)
                                                                                       aaCGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAAt     >  1:4354736/1‑93 (MQ=255)
                                                                                       aaCGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAAt     >  1:2483055/1‑93 (MQ=255)
                                                                                        aCGCGC‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATc    <  2:2769913/93‑1 (MQ=255)
                                                                                             |                                                                                          
CGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCT  >  NC_000913/2184589‑2184770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑