BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,115,116	+C	coding (2013/2028 nt)	b2974 ←	putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,115,115	1	.	C	99.7%	978.4 / ‑4.6	283	coding (2014/2028 nt)	b2974	putative endoglucanase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (136/146); total (137/146)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115023‑3115202

gTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAttt                                                                                          <  2:2939828/93‑1 (MQ=255)

 tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑c                                                                                        <  1:6041869/93‑1 (MQ=255)

 tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGCCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑c                                                                                        <  1:6214194/93‑1 (MQ=255)

  ttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cc                                                                                       >  1:1464029/1‑92 (MQ=255)

   tGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cct                                                                                      <  1:6439455/93‑3 (MQ=255)

    gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cctg                                                                                     <  2:7043541/93‑4 (MQ=255)

    gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cctg                                                                                     <  2:1216000/93‑4 (MQ=255)

    gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cctg                                                                                     <  1:2056720/93‑4 (MQ=255)

     cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cctgc                                                                                    <  1:7189896/93‑5 (MQ=255)

       ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCgg                                                                                   >  2:1913770/1‑93 (MQ=255)

       ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCgg                                                                                   >  1:5641088/1‑93 (MQ=255)

         tATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAt                                                                                 >  2:4824739/1‑93 (MQ=255)

          aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATc                                                                                <  2:4125673/93‑1 (MQ=255)

          aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATc                                                                                <  1:6946795/93‑1 (MQ=255)

             tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCgag                                                                             >  1:7401641/1‑93 (MQ=255)

              gCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCgaga                                                                            <  2:1834350/93‑1 (MQ=255)

              gCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCgaga                                                                            >  1:5508866/1‑93 (MQ=255)

               cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGAt                                                                           <  1:4691371/93‑1 (MQ=255)

                aCGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATc                                                                          <  1:1938702/93‑1 (MQ=255)

                 cGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCg                                                                         <  2:6288169/93‑1 (MQ=255)

                  gCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGc                                                                        <  2:6774514/93‑1 (MQ=255)

                   cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGcc                                                                       >  1:4202854/1‑93 (MQ=255)

                     gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAt                                                                     >  2:2706622/1‑93 (MQ=255)

                     gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAt                                                                     >  1:7056566/1‑93 (MQ=255)

                      cGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATa                                                                    <  2:461557/93‑1 (MQ=255)

                      cGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATa                                                                    <  2:5136230/93‑1 (MQ=255)

                        ggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGc                                                                  >  2:928185/1‑93 (MQ=255)

                          aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTa                                                                <  2:7297729/93‑1 (MQ=255)

                           ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAc                                                               >  1:789143/1‑93 (MQ=255)

                           ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAc                                                               >  1:6836118/1‑93 (MQ=255)

                           ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAc                                                               >  1:4020381/1‑93 (MQ=255)

                            tGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACt                                                              <  2:1978541/93‑1 (MQ=255)

                             gCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACtt                                                             >  2:2784786/1‑93 (MQ=255)

                              cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                            >  2:1491491/1‑93 (MQ=255)

                              cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                            >  2:5533492/1‑93 (MQ=255)

                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGTGGATCGAGATCGCCATAGCTACTTaaa                                                          <  2:6416899/93‑1 (MQ=255)

                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          <  1:1335587/93‑1 (MQ=255)

                                 aaGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaann                                                         >  1:3636844/1‑91 (MQ=255)

                                   ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                       <  1:3507661/93‑1 (MQ=255)

                                   ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                       <  2:309871/93‑1 (MQ=255)

                                    gATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCt                                                      >  2:1096911/1‑93 (MQ=255)

                                    gATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCt                                                      >  1:3767229/1‑93 (MQ=255)

                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  2:4522392/93‑1 (MQ=255)

                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  1:1790295/93‑1 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    >  1:524190/1‑93 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    >  1:2229664/1‑93 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    >  1:1211848/1‑93 (MQ=255)

                                      tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    >  1:3006286/1‑93 (MQ=255)

                                       cGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCaa                                                   <  1:6141767/93‑1 (MQ=255)

                                        gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  >  1:431322/1‑93 (MQ=255)

                                        gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  <  2:683737/93‑1 (MQ=255)

                                          tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  1:2723436/93‑1 (MQ=255)

                                          tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  1:3845165/93‑1 (MQ=255)

                                          tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  2:740286/93‑1 (MQ=255)

                                           aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               >  2:2169395/1‑93 (MQ=255)

                                           aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               >  2:4916518/1‑93 (MQ=255)

                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  1:3159366/93‑1 (MQ=255)

                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  2:5890076/93‑1 (MQ=255)

                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             >  2:6149023/1‑93 (MQ=255)

                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  1:4477647/93‑1 (MQ=255)

                                              cACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAt                                            <  1:1990367/93‑1 (MQ=255)

                                              cACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAt                                            <  2:6701902/93‑1 (MQ=255)

                                                ccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                          <  2:2549985/93‑1 (MQ=255)

                                                ccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                          <  1:6174927/93‑1 (MQ=255)

                                                ccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                          >  2:1306669/1‑93 (MQ=255)

                                                ccTGAGTCACATATACAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                          <  1:1595701/93‑1 (MQ=255)

                                                 cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                         <  1:5808910/93‑1 (MQ=255)

                                                  tGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                        >  1:2371507/1‑93 (MQ=255)

                                                     gTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCa                                     >  1:6715154/1‑93 (MQ=255)

                                                     gTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCa                                     >  1:872629/1‑93 (MQ=255)

                                                      tCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaa                                    >  2:6862484/1‑93 (MQ=255)

                                                       cacaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaa                                   <  2:3715812/93‑1 (MQ=255)

                                                        acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  >  1:2592801/1‑93 (MQ=255)

                                                        acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  <  1:5943156/93‑1 (MQ=255)

                                                        acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  >  1:3066201/1‑93 (MQ=255)

                                                          atatTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATc                                >  2:2750945/1‑93 (MQ=255)

                                                              tCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                            <  2:2999001/93‑1 (MQ=255)

                                                              tCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                            <  1:2814070/93‑1 (MQ=255)

                                                               cAAAGCCGATAAGGATCAGCAGCTGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAt                           <  1:4859716/93‑1 (MQ=255)

                                                                aaaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATg                          <  1:1017117/93‑1 (MQ=255)

                                                                aaaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATg                          >  2:5277497/1‑93 (MQ=255)

                                                                aaaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATg                          >  1:174087/1‑93 (MQ=255)

                                                                   gCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                       >  1:625066/1‑93 (MQ=255)

                                                                    ccGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                      <  2:3388817/93‑1 (MQ=255)

                                                                     cGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGt                     <  1:46061/93‑1 (MQ=255)

                                                                      gTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    <  2:80236/93‑1 (MQ=255)

                                                                       ttAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  2:3036192/1‑93 (MQ=255)

                                                                         aaGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  1:4974191/93‑1 (MQ=255)

                                                                          aGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                <  1:5409340/93‑1 (MQ=255)

                                                                             aTCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  1:324410/93‑1 (MQ=255)

                                                                             aTCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  2:5727316/93‑1 (MQ=255)

                                                                              tCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAg            >  1:6903975/1‑93 (MQ=255)

                                                                              tCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAg            <  2:4335032/93‑1 (MQ=255)

                                                                               cagcagCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc           <  2:3527707/93‑1 (MQ=255)

                                                                               cagcagCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc           <  2:2277550/93‑1 (MQ=255)

                                                                                agcagcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcg          >  2:3304716/1‑93 (MQ=255)

                                                                                 gcagcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgc         <  2:3491885/93‑1 (MQ=255)

                                                                                       gCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTc   <  1:6640717/93‑1 (MQ=255)

                                                                                        cATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTcc  <  2:1513983/93‑1 (MQ=255)

                                                                                        cATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTcc  >  2:3915145/1‑93 (MQ=255)

GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115023‑3115202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`