BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,926	+C	coding (1052/1434 nt)	ygjI →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,924	1	.	C	99.7%	970.1 / ‑4.1	285	coding (1050/1434 nt)	ygjI	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (141/143); total (142/143)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 316 total aligned reads displayed.

CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224831‑3225011

 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑c                                                                                         >  2:4356479/1‑93 (MQ=255)

 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑c                                                                                         >  1:2249426/1‑93 (MQ=255)

 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑c                                                                                         >  2:4332192/1‑93 (MQ=255)

  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑c‑c                                                                                       >  1:5308144/1‑93 (MQ=255)

     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑c‑ccga                                                                                    >  2:3124373/1‑90 (MQ=255)

     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑c‑ccga                                                                                    >  1:1018789/1‑90 (MQ=255)

     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑c‑ccga                                                                                    <  2:3399439/93‑4 (MQ=255)

      tAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGGGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCCGCTGATGATTAT‑c‑ccga                                                                                    <  1:3652249/93‑4 (MQ=255)

          aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTc                                                                                <  2:2999543/93‑1 (MQ=255)

          aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTc                                                                                >  1:5881760/1‑93 (MQ=255)

           aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCg                                                                               <  1:3418383/93‑1 (MQ=255)

            aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCgg                                                                              >  2:4895682/1‑93 (MQ=255)

               cGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTc                                                                           >  1:4079967/1‑93 (MQ=255)

                gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTcc                                                                          >  2:2166688/1‑93 (MQ=255)

                gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTcc                                                                          >  2:3701422/1‑93 (MQ=255)

                 tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCa                                                                         >  1:2548214/1‑93 (MQ=255)

                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATa                                                                      <  2:4014625/93‑1 (MQ=255)

                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATa                                                                      >  1:1986427/1‑93 (MQ=255)

                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATa                                                                      >  2:5504665/1‑93 (MQ=255)

                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATa                                                                      <  2:5826152/93‑1 (MQ=255)

                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATAc                                                                     <  2:2334003/93‑1 (MQ=255)

                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATAcc                                                                    >  2:6978221/1‑93 (MQ=255)

                       tGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCg                                                                   <  2:32382/93‑1 (MQ=255)

                       tGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCg                                                                   >  2:6878305/1‑93 (MQ=255)

                       tGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCg                                                                   >  2:2859499/1‑93 (MQ=255)

                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGt                                                                  <  1:1031654/93‑1 (MQ=255)

                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGt                                                                  >  1:6177819/1‑93 (MQ=255)

                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGt                                                                  >  2:2629565/1‑93 (MQ=255)

                         aaCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTg                                                                 <  1:501314/93‑1 (MQ=255)

                         aaCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTg                                                                 >  2:116613/1‑93 (MQ=255)

                          aCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGc                                                                <  2:650116/93‑1 (MQ=255)

                          aCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGc                                                                <  1:3122236/93‑1 (MQ=255)

                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAg                                                              >  1:3915600/1‑93 (MQ=255)

                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAg                                                              <  1:1475589/93‑1 (MQ=255)

                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAg                                                              >  2:6280524/1‑93 (MQ=255)

                             aaaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAgg                                                             >  2:4123387/1‑93 (MQ=255)

                              aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGa                                                            <  2:4446936/93‑1 (MQ=255)

                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATc                                                          >  1:7129614/1‑93 (MQ=255)

                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATc                                                          <  1:3184425/93‑1 (MQ=255)

                                  ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCtg                                                        >  2:245237/1‑93 (MQ=255)

                                      ttCGGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                                    >  1:6974177/1‑93 (MQ=255)

                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                                    >  1:2200489/1‑93 (MQ=255)

                                       tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                                                   >  1:2902822/1‑93 (MQ=255)

                                        ccGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt                                                  <  2:564261/93‑1 (MQ=255)

                                        ccGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt                                                  <  1:5472368/93‑1 (MQ=255)

                                         cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                                 <  2:6159035/93‑1 (MQ=255)

                                             cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                                              <  2:5133449/90‑1 (MQ=255)

                                             cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                                              <  2:1559020/90‑1 (MQ=255)

                                                gcgcAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                           <  2:2794838/93‑1 (MQ=255)

                                                 cgcAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat                                          <  1:1088682/93‑1 (MQ=255)

                                                   cAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                        <  1:1302580/93‑1 (MQ=255)

                                                    aGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       <  2:6390013/93‑1 (MQ=255)

                                                     gCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      >  1:561174/1‑93 (MQ=255)

                                                     gCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      <  1:3548023/93‑1 (MQ=255)

                                                     gCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      <  1:3980648/93‑1 (MQ=255)

                                                     gCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      >  2:732906/1‑93 (MQ=255)

                                                      cGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                     <  2:7040364/93‑1 (MQ=255)

                                                      cGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                     <  1:5271413/93‑1 (MQ=255)

                                                      cGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                     <  2:168376/93‑1 (MQ=255)

                                                       gTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  1:809491/93‑1 (MQ=255)

                                                       gTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    >  2:7269069/1‑93 (MQ=255)

                                                       gTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  2:592521/93‑1 (MQ=255)

                                                       gTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  1:907280/93‑1 (MQ=255)

                                                       gTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  1:683852/93‑1 (MQ=255)

                                                         ggATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                  <  1:1798088/93‑1 (MQ=255)

                                                          gATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                 <  2:4778271/93‑1 (MQ=255)

                                                           aTCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc                                <  1:3275582/93‑1 (MQ=255)

                                                           aTCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc                                >  1:6670520/1‑93 (MQ=255)

                                                            tCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑CACGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                               >  1:5990741/1‑93 (MQ=255)

                                                              cAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc                             >  1:7006906/1‑93 (MQ=255)

                                                              cAGTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc                             <  1:5556551/93‑1 (MQ=255)

                                                                gTTCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACcgccgc                           >  1:4275079/1‑93 (MQ=255)

                                                                 ttCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          >  1:2045964/1‑93 (MQ=255)

                                                                 ttCCTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          <  2:3509656/93‑1 (MQ=255)

                                                                   ccTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGc                        <  2:5960936/93‑1 (MQ=255)

                                                                    cTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCg                       <  1:6501510/93‑1 (MQ=255)

                                                                    cTGATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCg                       >  2:6587644/1‑93 (MQ=255)

                                                                      gATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTc                     <  1:2980602/93‑1 (MQ=255)

                                                                      gATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTc                     >  2:600549/1‑93 (MQ=255)

                                                                      gATCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTc                     >  2:5398232/1‑93 (MQ=255)

                                                                       aTCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTcc                    >  2:901222/1‑93 (MQ=255)

                                                                        tCGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                   >  2:1285569/1‑93 (MQ=255)

                                                                         cgtcgt‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCGATACCGTGCAGGATCTGATGAATCCTATTATAAATATGACCGCCGCAGCGTCCAt                  <  2:1904576/93‑1 (MQ=255)

                                                                         cGTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAt                  <  1:1738331/93‑1 (MQ=255)

                                                                          gTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATg                 >  1:4534719/1‑93 (MQ=255)

                                                                          gTCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATg                 >  2:5224434/1‑93 (MQ=255)

                                                                           tCAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGc                >  1:2724961/1‑93 (MQ=255)

                                                                            cAT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGGAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt               <  1:7302485/93‑1 (MQ=255)

                                                                             aT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt              >  1:3779865/1‑93 (MQ=255)

                                                                             aT‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt              >  1:1409086/1‑93 (MQ=255)

                                                                              t‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             >  1:6232925/1‑93 (MQ=255)

                                                                              t‑CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             <  2:6680753/93‑1 (MQ=255)

                                                                                  cGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgc          >  1:2393582/1‑93 (MQ=255)

                                                                                  cGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgc          >  1:7456501/1‑93 (MQ=255)

                                                                                    cTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgccg        <  2:3849517/93‑1 (MQ=255)

                                                                                     tgatgaTTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGt       >  2:5716521/1‑93 (MQ=255)

                                                                                     tgatgaTTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCAGCCGt       >  1:4919193/1‑93 (MQ=255)

                                                                                      gatgatTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      <  1:1167165/93‑1 (MQ=255)

                                                                                       atgatTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTa     >  2:1521926/1‑93 (MQ=255)

                                                                                        tgatTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt    >  1:515569/1‑93 (MQ=255)

CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCAT‑CCCGCTGATGATTAT‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224831‑3225011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`