Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,235,656 (G)5→6 coding (444/2097 nt) yjbH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,235,6511.G81.8% 616.9 / 136.4 220coding (439/2097 nt)yjbHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (18/22);  new base G (88/92);  total (106/114)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 263 total aligned reads displayed.

AGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAA  >  NC_000913/4235559‑4235743
                                                                                             |                                                                                             
agagTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCt                                                                                                <  1:4569952/93‑1 (MQ=255)
agagTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCt                                                                                                <  2:2728797/93‑1 (MQ=255)
agagTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCt                                                                                                >  1:400972/1‑93 (MQ=255)
agagTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCt                                                                                                >  2:4180972/1‑93 (MQ=255)
agagTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCt                                                                                                <  2:6564922/93‑1 (MQ=255)
 gagTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑g                                                                                             >  2:962056/1‑93 (MQ=255)
  agTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑gg                                                                                            <  2:682272/93‑1 (MQ=255)
  agTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑gg                                                                                            <  1:4878606/93‑1 (MQ=255)
  agTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑gg                                                                                            <  1:1284370/93‑1 (MQ=255)
   gTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑ggg                                                                                           >  1:4786035/1‑93 (MQ=255)
   gTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑ggg                                                                                           <  2:3398898/93‑1 (MQ=255)
   gTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑ggg                                                                                           <  1:2324648/93‑1 (MQ=255)
    ttACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑gggg                                                                                          <  2:4727436/93‑1 (MQ=255)
    ttACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑gggg                                                                                          <  2:4252202/93‑1 (MQ=255)
    ttACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑gggg                                                                                          >  2:7005953/1‑93 (MQ=255)
      aCTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑gggggg                                                                                        <  1:2250849/93‑2 (MQ=255)
            tGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTc                                                                                   >  2:3359675/1‑93 (MQ=255)
               cGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGAt                                                                                >  2:4870382/1‑93 (MQ=255)
                 cAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGAttt                                                                              >  1:1571590/1‑93 (MQ=255)
                 cAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGAttt                                                                              >  2:4974731/1‑93 (MQ=255)
                   aGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTa                                                                            <  1:1073974/93‑1 (MQ=255)
                   aGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTAc                                                                           >  1:653739/1‑93 (MQ=255)
                     tGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACg                                                                          >  2:3773725/1‑93 (MQ=255)
                     tGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACg                                                                          <  2:3737468/93‑1 (MQ=255)
                     tGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGc                                                                         >  2:1384052/1‑93 (MQ=255)
                       gCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTc                                                                       <  2:5239082/93‑1 (MQ=255)
                         ggTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCg                                                                      >  2:1512694/1‑93 (MQ=255)
                         ggTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCg                                                                      <  2:3557496/93‑1 (MQ=255)
                          gTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCgg                                                                     >  1:5635995/1‑93 (MQ=255)
                           ttGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGc                                                                    >  2:2515530/1‑93 (MQ=255)
                           ttGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGc                                                                    >  2:3950913/1‑93 (MQ=255)
                             ggTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCct                                                                  <  2:7195025/93‑1 (MQ=255)
                             ggTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCct                                                                  >  2:4320746/1‑93 (MQ=255)
                              gTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCctg                                                                 <  1:5231337/93‑1 (MQ=255)
                              gTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCctg                                                                 >  2:2602095/1‑93 (MQ=255)
                              gTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCctgg                                                                <  2:1229193/93‑1 (MQ=255)
                               tGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCctgg                                                                <  1:5379347/93‑1 (MQ=255)
                               tGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCctggg                                                               >  1:637033/1‑93 (MQ=255)
                                   cgGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCctgggctgg                                                           <  2:4563657/93‑1 (MQ=255)
                                    gggATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCctgggctgg                                                           <  1:4721726/93‑1 (MQ=255)
                                     ggATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCctgggctggg                                                          >  2:6975701/1‑93 (MQ=255)
                                     ggATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCctgggctggg                                                          <  2:1706567/93‑1 (MQ=255)
                                        tatCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGt                                                       >  2:182038/1‑93 (MQ=255)
                                        tatCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGt                                                       >  1:7250452/1‑93 (MQ=255)
                                          tCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTGGGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTa                                                      >  2:5120862/1‑93 (MQ=255)
                                          tCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGCAt                                                     >  1:5846978/1‑93 (MQ=255)
                                          tCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTAtt                                                    >  1:4456290/1‑93 (MQ=255)
                                           cggcggTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTAtt                                                    <  2:3026043/93‑1 (MQ=255)
                                            ggcggTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTAttt                                                   <  2:588901/93‑1 (MQ=255)
                                             gcggTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTg                                                  >  1:4073459/1‑93 (MQ=255)
                                             gcggTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTg                                                  >  1:7173930/1‑93 (MQ=255)
                                                gTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGc                                               <  2:1954345/93‑1 (MQ=255)
                                                gTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGc                                               >  1:522432/1‑93 (MQ=255)
                                                 tACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCa                                              >  1:1365311/1‑93 (MQ=255)
                                                 tACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCa                                              >  2:34740/1‑93 (MQ=255)
                                                 tACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCa                                              <  1:3911478/93‑1 (MQ=255)
                                                 tACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGGATTTGGGCa                                              >  1:2646009/1‑93 (MQ=255)
                                                   cGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCAcc                                            <  1:956376/93‑1 (MQ=255)
                                                    ggggCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCa                                           >  1:4910460/1‑93 (MQ=255)
                                                    ggggCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCa                                           <  1:594775/93‑1 (MQ=255)
                                                    ggggCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCa                                           >  1:2029917/1‑93 (MQ=255)
                                                     gggCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAg                                          >  1:4264301/1‑93 (MQ=255)
                                                      ggCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTGGGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAg                                          >  2:479885/1‑93 (MQ=255)
                                                      ggCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCg                                        >  2:2914884/1‑93 (MQ=255)
                                                      ggCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCg                                        <  2:3150402/93‑1 (MQ=255)
                                                        cTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCgg                                       <  2:56241/93‑1 (MQ=255)
                                                         tGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGt                                      <  2:7028874/93‑1 (MQ=255)
                                                         tGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGt                                      <  2:5035659/93‑1 (MQ=255)
                                                         tGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGt                                      >  2:5791808/1‑93 (MQ=255)
                                                         tGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGt                                      >  2:2876809/1‑93 (MQ=255)
                                                           tttGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTaa                                    >  2:6523798/1‑93 (MQ=255)
                                                            ttGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAAt                                   <  1:6132783/93‑1 (MQ=255)
                                                             tGATGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAAtg                                  <  2:6069273/93‑1 (MQ=255)
                                                             tGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAAtgt                                 <  1:805770/93‑1 (MQ=255)
                                                             tGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAAtgt                                 <  1:239210/93‑1 (MQ=255)
                                                                tGCGGAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGa                               >  2:2319828/1‑93 (MQ=255)
                                                                    gAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGaaaaa                           >  1:6726430/1‑93 (MQ=255)
                                                                    gAATATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGGATTTGGGCACCAGCGGTAATGTGaaaaa                           >  2:5986395/1‑93 (MQ=255)
                                                                     aaTATCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAAt                          <  2:2133163/93‑1 (MQ=255)
                                                                      atatCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATc                         >  2:1299167/1‑93 (MQ=255)
                                                                      atatCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATc                         <  2:3194432/93‑1 (MQ=255)
                                                                      atatCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATc                         <  1:2409420/93‑1 (MQ=255)
                                                                        atCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCg                       >  1:1184536/1‑93 (MQ=255)
                                                                        atCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGCATTTGGGCACCAGCGGTAATGTGAAAAATCCg                       >  1:3975790/1‑93 (MQ=255)
                                                                         tCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGc                      <  1:5065398/93‑1 (MQ=255)
                                                                         tCTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGc                      <  1:1636482/93‑1 (MQ=255)
                                                                          cTTGTTGCCAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGct                     <  2:5544204/93‑1 (MQ=255)
                                                                                 ccAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCa              <  1:7513325/93‑1 (MQ=255)
                                                                                 ccAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCa              <  1:3059325/93‑1 (MQ=255)
                                                                                  cAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAg             <  1:3607523/93‑1 (MQ=255)
                                                                                  cAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAg             >  1:1666129/1‑93 (MQ=255)
                                                                                  cAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAg             <  1:2900865/93‑1 (MQ=255)
                                                                                  cAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAg             >  2:7468481/1‑93 (MQ=255)
                                                                                  cAGCAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAg             >  1:2875673/1‑93 (MQ=255)
                                                                                     cAAAGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCa          <  1:980683/93‑1 (MQ=255)
                                                                                      aaaGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAg         >  1:5703282/1‑93 (MQ=255)
                                                                                       aaGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGt        <  1:582629/93‑1 (MQ=255)
                                                                                       aaGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGt        <  2:5679727/93‑1 (MQ=255)
                                                                                        aGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTg       <  2:6239646/93‑1 (MQ=255)
                                                                                        aGCCTG‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTg       <  2:5199816/93‑1 (MQ=255)
                                                                                             |                                                                                             
AGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGTACGGGGCTGTTTGATGCGGAATATCTTGTTGCCAGCAAAGCCT‑‑GGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAA  >  NC_000913/4235559‑4235743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑