BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,684	+A	coding (400/501 nt)	ytfI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,681	1	.	A	100.0%	1185.1 / NA	302	Q133K (CAA→AAA)	ytfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (138/164); total (138/164)

Only 100 of 325 total aligned reads displayed.

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

 aGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑a                                                                                        <  1:2694853/93‑1 (MQ=255)

  ggTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aa                                                                                       <  1:5049195/93‑1 (MQ=255)

     aGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaac                                                                                    <  1:5280523/93‑3 (MQ=255)

     aGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaac                                                                                    <  2:1191830/93‑3 (MQ=255)

       tGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaacag                                                                                  >  1:7154868/1‑89 (MQ=255)

         gATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGAc                                                                                 <  2:103377/93‑1 (MQ=255)

             tGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGAtt                                                                             <  1:1177382/93‑1 (MQ=255)

             tGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGAtt                                                                             <  1:4382939/93‑1 (MQ=255)

             tGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGAtt                                                                             <  2:2913669/93‑1 (MQ=255)

              gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTg                                                                            <  1:4138407/93‑1 (MQ=255)

              gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTg                                                                            <  1:910516/93‑1 (MQ=255)

               aaTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGt                                                                           <  2:791225/93‑1 (MQ=255)

               aaTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGt                                                                           <  2:2642101/93‑1 (MQ=255)

                aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTa                                                                          >  1:488861/1‑93 (MQ=255)

                 tACGAAGAAATATCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         <  1:7023906/93‑1 (MQ=255)

                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         <  1:1859684/93‑1 (MQ=255)

                  aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATg                                                                        >  2:2152288/1‑93 (MQ=255)

                  aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATg                                                                        >  1:1038638/1‑93 (MQ=255)

                    gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                      <  2:6831003/93‑1 (MQ=255)

                     aagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCt                                                                     <  2:315629/93‑1 (MQ=255)

                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    <  1:2771436/93‑1 (MQ=255)

                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    <  1:665214/93‑1 (MQ=255)

                       gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTgg                                                                   <  1:6475157/93‑1 (MQ=255)

                       gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTgg                                                                   <  1:5578288/93‑1 (MQ=255)

                         aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGa                                                                 <  2:4798976/93‑1 (MQ=255)

                           tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGata                                                               >  1:604734/1‑93 (MQ=255)

                           tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGata                                                               <  2:960629/93‑1 (MQ=255)

                             cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATAtt                                                             >  1:4050934/1‑93 (MQ=255)

                             cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATAtt                                                             >  2:238972/1‑93 (MQ=255)

                                aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACAATTGTATGCCTGGGATATTCTa                                                          <  2:5171101/93‑1 (MQ=255)

                                 tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTAc                                                         >  1:7237731/1‑93 (MQ=255)

                                   aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAg                                                       >  2:7339692/1‑93 (MQ=255)

                                   aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAg                                                       >  2:2516000/1‑93 (MQ=255)

                                     aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaa                                                     <  2:3455711/93‑1 (MQ=255)

                                     aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaa                                                     >  1:3962973/1‑93 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  2:3723585/1‑93 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaa                                                   <  2:1448718/93‑1 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  1:3739187/1‑93 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 >  1:143974/1‑93 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 >  2:7279371/1‑93 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  2:3877799/93‑1 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  1:2125409/93‑1 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  1:4704050/93‑1 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                >  1:5732053/1‑93 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                >  2:6041791/1‑93 (MQ=255)

                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               >  1:5926855/1‑93 (MQ=255)

                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               >  2:5395686/1‑93 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGTTATTTTACAGAAAAAACAg                                              >  2:3081329/1‑93 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              <  2:87538/93‑1 (MQ=255)

                                             aaTATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                             >  1:3530309/1‑93 (MQ=255)

                                             aaTATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                             >  2:4642784/1‑93 (MQ=255)

                                              atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                            <  2:2855473/93‑1 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           >  2:2019782/1‑93 (MQ=255)

                                                ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          >  1:4268076/1‑93 (MQ=255)

                                                ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          >  1:2230532/1‑93 (MQ=255)

                                                ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          >  1:3807391/1‑93 (MQ=255)

                                                 taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                         <  1:2024630/93‑1 (MQ=255)

                                                  aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGCTTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                        <  2:7381704/93‑1 (MQ=255)

                                                  aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                        <  2:4529626/93‑1 (MQ=255)

                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       <  2:4125322/93‑1 (MQ=255)

                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       <  1:306918/93‑1 (MQ=255)

                                                    cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                      >  2:2342808/1‑93 (MQ=255)

                                                    cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                      >  1:3130222/1‑93 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  2:176437/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  1:3592557/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  1:1563575/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     <  1:5146254/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     >  1:2421080/1‑93 (MQ=255)

                                                      cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                    >  2:2263022/1‑93 (MQ=255)

                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   <  1:1905650/93‑1 (MQ=255)

                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  >  2:293640/1‑93 (MQ=255)

                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  >  2:114669/1‑93 (MQ=255)

                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  >  2:3360511/1‑93 (MQ=255)

                                                          catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                >  1:6807797/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               <  1:5813552/93‑1 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  1:5981451/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  1:1984901/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  2:5464140/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  2:6441494/1‑93 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              >  1:7515476/1‑93 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              >  2:1887912/1‑93 (MQ=255)

                                                               aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                           <  2:6757398/93‑1 (MQ=255)

                                                                  tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                        <  2:6948522/93‑1 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     <  2:6333570/93‑1 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     >  1:613117/1‑93 (MQ=255)

                                                                      aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                    >  2:7075470/1‑93 (MQ=255)

                                                                       aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                   <  2:4399674/93‑1 (MQ=255)

                                                                        aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGtt                  >  2:5718147/1‑93 (MQ=255)

                                                                         ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                 <  2:5915866/93‑1 (MQ=255)

                                                                         ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                 <  1:6308811/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  2:1325151/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  2:4939538/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  1:4748399/93‑1 (MQ=255)

                                                                            tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat              >  1:6894501/1‑93 (MQ=255)

                                                                               aTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACACTCTGACATACCGAAAAGGATTAAAGGTTATATTCa           <  1:5693979/93‑1 (MQ=255)

                                                                                  tCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        <  1:4601111/93‑1 (MQ=255)

                                                                                    cGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg      >  1:98480/1‑93 (MQ=255)

                                                                                     gTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc     >  2:5785938/1‑93 (MQ=255)

                                                                                     gTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTAAATTCACCAGGc     <  1:2300296/93‑1 (MQ=255)

                                                                                      ttAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    >  2:5236180/1‑93 (MQ=255)

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`