BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,449,738	+G	coding (1106/1254 nt)	ytfR →	YtfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,449,737	1	.	G	99.3%	1024.8 / ‑2.0	302	coding (1105/1254 nt)	ytfR	YtfR
Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (138/162); total (138/164)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 312 total aligned reads displayed.

GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAAC  >  NC_000913/4449645‑4449823

gCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct                                                                                          <  2:4665021/93‑1 (MQ=255)

   tCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggc                                                                                      >  1:2761371/1‑91 (MQ=255)

   tCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggc                                                                                      <  1:2128828/93‑3 (MQ=255)

     tGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggcta                                                                                    <  1:5902473/93‑5 (MQ=255)

        ccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGt                                                                                 >  2:7528309/1‑93 (MQ=255)

         cGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTc                                                                                >  1:6722074/1‑93 (MQ=255)

         cGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTc                                                                                >  2:1771890/1‑93 (MQ=255)

          ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTcc                                                                               <  2:6942051/93‑1 (MQ=255)

           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTccc                                                                              >  2:2635795/1‑93 (MQ=255)

            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCa                                                                             <  2:5575030/93‑1 (MQ=255)

            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCa                                                                             <  2:4366895/93‑1 (MQ=255)

             aGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCAt                                                                            <  1:3202122/93‑1 (MQ=255)

             aGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCAt                                                                            >  1:3729199/1‑93 (MQ=255)

                 aGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTcc                                                                        <  1:1246183/93‑1 (MQ=255)

                  ggAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTccc                                                                       >  2:5697651/1‑93 (MQ=255)

                  ggAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTccc                                                                       >  1:6031912/1‑93 (MQ=255)

                  ggAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTccc                                                                       >  1:5271035/1‑93 (MQ=255)

                   gAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCg                                                                      >  2:7166068/1‑93 (MQ=255)

                       aCCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCaaa                                                                  <  2:5263237/93‑1 (MQ=255)

                       aCCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCaaa                                                                  <  2:6278967/93‑1 (MQ=255)

                         cGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGa                                                                <  2:3270932/93‑1 (MQ=255)

                         cGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGa                                                                <  1:548919/93‑1 (MQ=255)

                          gATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGaa                                                               >  2:642767/1‑93 (MQ=255)

                           aTGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCATGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAAc                                                              <  1:1119478/93‑1 (MQ=255)

                           aTGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAAc                                                              >  2:4724316/1‑93 (MQ=255)

                           aTGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAAc                                                              >  2:5660531/1‑93 (MQ=255)

                           aTGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAAc                                                              >  1:1747896/1‑93 (MQ=255)

                            tGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACa                                                             <  2:3434314/93‑1 (MQ=255)

                             ggCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAg                                                            <  1:6856071/93‑1 (MQ=255)

                               catcatCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCa                                                          <  2:2555260/93‑1 (MQ=255)

                               catcatCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCa                                                          >  2:5139586/1‑93 (MQ=255)

                               catcatCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCa                                                          <  2:2959277/93‑1 (MQ=255)

                                atcatcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCaa                                                         >  2:197294/1‑93 (MQ=255)

                                atcatcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCaa                                                         >  2:164481/1‑93 (MQ=255)

                                atcatcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCaa                                                         <  1:1899056/93‑1 (MQ=255)

                                atcatcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCaa                                                         <  1:1386134/93‑1 (MQ=255)

                                  catcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAaga                                                       >  1:1589250/1‑93 (MQ=255)

                                  catcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAaga                                                       <  1:4151109/93‑1 (MQ=255)

                                   atcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAagag                                                      >  2:211730/1‑93 (MQ=255)

                                   atcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAagag                                                      >  2:3386165/1‑93 (MQ=255)

                                   atcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAagag                                                      <  1:4736641/93‑1 (MQ=255)

                                    tcGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                     >  1:3037310/1‑93 (MQ=255)

                                     cGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                    <  2:5031641/93‑1 (MQ=255)

                                     cGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                    <  2:3589327/93‑1 (MQ=255)

                                      gCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAtt                                                   <  1:2538332/93‑1 (MQ=255)

                                       cTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                  >  1:7059078/1‑93 (MQ=255)

                                        tGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGc                                                 >  2:2417087/1‑93 (MQ=255)

                                         gccgccTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGcc                                                >  1:538610/1‑93 (MQ=255)

                                            gccTCGGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAAAAGCAAGAGATTGCCGa                                              >  1:2373697/1‑93 (MQ=255)

                                            gccTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                             <  2:6665127/93‑1 (MQ=255)

                                               tC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGc                                          >  1:3306572/1‑93 (MQ=255)

                                               tC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGc                                          >  1:5636240/1‑93 (MQ=255)

                                                  ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCtt                                        <  1:109566/93‑1 (MQ=255)

                                                   gTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCttt                                       <  1:5026571/93‑1 (MQ=255)

                                                   gTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCttt                                       <  2:3162162/93‑1 (MQ=255)

                                                    tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                      <  1:4458822/93‑1 (MQ=255)

                                                    tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                      <  2:1317669/93‑1 (MQ=255)

                                                    tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                      <  1:4086343/93‑1 (MQ=255)

                                                    tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                      <  1:4000024/93‑1 (MQ=255)

                                                      cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                    <  2:2265020/93‑1 (MQ=255)

                                                       gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                   <  2:4004042/93‑1 (MQ=255)

                                                       gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                   >  1:6220745/1‑93 (MQ=255)

                                                       gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                   >  1:636034/1‑93 (MQ=255)

                                                       gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                   >  2:5875584/1‑93 (MQ=255)

                                                        ggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                  >  2:3562704/1‑93 (MQ=255)

                                                        ggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                  >  1:2233181/1‑93 (MQ=255)

                                                            aaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAg                              >  2:5420322/1‑93 (MQ=255)

                                                            aaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAg                              >  1:6943660/1‑93 (MQ=255)

                                                             atatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGc                             <  1:664122/93‑1 (MQ=255)

                                                              tatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                            <  2:2832720/93‑1 (MQ=255)

                                                               atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                           >  1:295946/1‑93 (MQ=255)

                                                               atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                           >  2:6162329/1‑93 (MQ=255)

                                                               atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                           >  1:6755934/1‑93 (MQ=255)

                                                                tcatcCTCGCTCTCCAGGCACAGCGCGGCT‑GCCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTg                          <  1:4652155/93‑1 (MQ=255)

                                                                 catcCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTgg                         >  1:4611106/1‑93 (MQ=255)

                                                                 catcCTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTgg                         <  1:361613/93‑1 (MQ=255)

                                                                    ccTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAt                      <  2:118727/93‑1 (MQ=255)

                                                                    ccTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAt                      >  2:2776454/1‑93 (MQ=255)

                                                                     cTCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                     <  2:3733756/93‑1 (MQ=255)

                                                                      tCGCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATACGCCCGCTTGGCATTc                    >  1:4923821/1‑93 (MQ=255)

                                                                        gCTCTCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                  <  1:6481849/93‑1 (MQ=255)

                                                                         ctctCCAGGCCCAGCGCGGCTGG‑TTACGTCCCATTTCCCGCAAAGAACAGCAAGGGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                 >  1:708376/1‑93 (MQ=255)

                                                                         ctctCCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                 <  2:2678477/93‑1 (MQ=255)

                                                                          tctcCAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcac                <  2:6499595/93‑1 (MQ=255)

                                                                             nnaGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc             <  1:2041247/91‑1 (MQ=255)

                                                                             ccAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc             >  2:7355049/1‑93 (MQ=255)

                                                                             ccAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc             >  1:4834176/1‑93 (MQ=255)

                                                                              cAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt            >  2:2710558/1‑93 (MQ=255)

                                                                              cAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt            >  2:1262369/1‑93 (MQ=255)

                                                                              cAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt            >  2:7444143/1‑93 (MQ=255)

                                                                              cAGGCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt            <  1:4208010/93‑1 (MQ=255)

                                                                                ggCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc          >  2:967429/1‑93 (MQ=255)

                                                                                ggCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc          >  1:7218397/1‑93 (MQ=255)

                                                                                 gCCCAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCa         <  1:3457681/93‑1 (MQ=255)

                                                                                  cccAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCaa        <  2:5981944/93‑1 (MQ=255)

                                                                                    cAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt      >  1:5981296/1‑93 (MQ=255)

                                                                                    cAGCGCGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt      <  2:3035709/93‑1 (MQ=255)

                                                                                      gcgcGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa    >  2:6748788/1‑93 (MQ=255)

                                                                                      gcgcGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa    >  1:7483263/1‑93 (MQ=255)

                                                                                      gcgcGGCTGG‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa    >  2:4824270/1‑93 (MQ=255)

GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTC‑GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑G‑CTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAAC  >  NC_000913/4449645‑4449823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`