BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,663	(G)_8→9	noncoding (72/88 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,655	1	.	G	80.5%	390.9 / 114.7	179	noncoding (80/88 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (24/11); new base G (101/43); total (125/54)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGT  >  NC_000913/4603563‑4603739

ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑Ga                                                                                         <  1:4245769/93‑1 (MQ=255)

ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑Ga                                                                                         >  1:7298552/1‑93 (MQ=255)

ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑Ga                                                                                         >  2:7340856/1‑93 (MQ=255)

 tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑g                                                                                     >  1:920709/1‑93 (MQ=255)

 tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑g                                                                                     <  2:2831996/93‑1 (MQ=255)

  aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gg                                                                                    <  2:5058782/93‑1 (MQ=255)

  aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gg                                                                                    >  1:5102239/1‑93 (MQ=255)

   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggg                                                                                   >  1:1195982/1‑93 (MQ=255)

   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggg                                                                                   >  1:636872/1‑93 (MQ=255)

    tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggg                                                                                  <  1:5337390/93‑1 (MQ=255)

    tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggg                                                                                  <  1:363356/93‑1 (MQ=255)

    tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggg                                                                                  <  1:6090605/93‑1 (MQ=255)

     gCTGGCATCCTGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggg                                                                                 <  1:1383841/93‑1 (MQ=255)

      cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggggg                                                                                <  1:4569384/93‑1 (MQ=255)

       tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggg                                                                               >  2:6539252/1‑93 (MQ=255)

       tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggg                                                                               >  2:5621770/1‑93 (MQ=255)

         gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggg                                                                             <  1:6801583/93‑2 (MQ=255)

         gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggg                                                                             >  2:1859437/1‑92 (MQ=255)

         gCATAATGCTAATTATAATAAATTTAAATCAGGGAAAAAAAAAAAAAGAAGAAAAAAAAAAGGAAGAAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggg                                                                             <  1:2779295/93‑2 (MQ=255)

           atcatGGTGATGATAATTAATTTGAAAGAGAGATAAAAAATCAAGGAAAAAACAAGAAAGAAAATAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggggggggga                                                                           <  2:5729301/93‑4 (MQ=255)

           atcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACt                                                                           >  2:6266112/1‑93 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggggac                                                                          >  2:5496140/1‑89 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggggac                                                                          >  2:15436/1‑89 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggggac                                                                          >  1:999841/1‑89 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggggggggact                                                                          >  2:60668/1‑89 (MQ=255)

                gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAAc                                                                      >  1:3816066/1‑93 (MQ=255)

                  tgatgaTAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAAccc                                                                    >  2:5238398/1‑93 (MQ=255)

                   gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAAcccc                                                                   >  2:2560793/1‑93 (MQ=255)

                    atgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAAccc                                                                    >  2:1977572/1‑93 (MQ=255)

                    atgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAAccc                                                                    <  2:7221573/93‑1 (MQ=255)

                      gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAAccccc                                                                  >  1:3919518/1‑93 (MQ=255)

                      gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCa                                                                 >  1:5921780/1‑93 (MQ=255)

                       atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCa                                                                 <  2:3399646/93‑1 (MQ=255)

                       atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCa                                                                 <  1:1073071/93‑1 (MQ=255)

                       aaaaTGAATTTGAATCAGGGATGAAAAATCAAGGAAAAAACAAGAAAGGAAGTAAAGATAATT‑‑GGGGCGGGG‑‑‑GGGGGGGGACTTGAACCCCCa                                                                 <  1:1096042/91‑1 (MQ=255)

                        tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCAc                                                                <  2:3223461/93‑1 (MQ=255)

                        tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGt                                                              >  1:1746595/1‑93 (MQ=255)

                        gaatgaatgTGAATAGGGGAAGAATAAGAAATGAAAAAACAAGAAAAGAAGGAAAAAAAAGT‑‑GGTGC‑‑GAGG‑GGGGGGGGTCTTGAACCCCCAc                                                                <  1:3482379/92‑1 (MQ=255)

                         aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACg                                                               >  2:7518050/1‑93 (MQ=39)

                          aTGAATTTGAATCAGGGATAAAAAATAAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGt                                                              <  2:7021360/93‑1 (MQ=38)

                              aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCg                                                           >  1:1516356/1‑93 (MQ=38)

                              aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGTCTTGAACCCCCACATCCGt                                                          >  2:2712305/1‑93 (MQ=255)

                                  gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGac                                                    <  1:4173190/93‑1 (MQ=255)

                                   aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                    <  2:1670583/93‑1 (MQ=255)

                                       aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                  >  1:1875737/1‑93 (MQ=37)

                                        gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                >  2:7081163/1‑93 (MQ=37)

                                        gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                >  1:2356797/1‑93 (MQ=37)

                                         ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTCGAACCCCCACGTCCGTAAGGACACTaa                                               >  2:4584679/1‑93 (MQ=37)

                                         ggATAAAAAACAAAAGAAGAAACAAGAAAGGAAGGAAAGATAAGG‑‑GGTGA‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                               <  2:4536861/93‑1 (MQ=17)

                                          gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                              <  2:7476318/93‑1 (MQ=37)

                                          aaaaaaaaaaaaAGGAAGAAAAAAAAAAGGAAGTAAAGATAATG‑‑GGGGG‑‑GAGGGGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTaa                                               <  2:1871109/89‑1 (MQ=12)

                                           ataaaaaaTAAAGGAAGAAAAAAAAAAGGAAGAAAAGAAAATTGGGGGGG‑‑GG‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca                                             <  1:5888013/90‑1 (MQ=12)

                                             gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                           >  2:6880182/1‑93 (MQ=25)

                                             aaaaaaaCAAGGAAGAAACAAGAAAGGAAGAAAAAAAAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                           <  1:662186/92‑1 (MQ=18)

                                                aaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa                                       <  2:4895380/93‑1 (MQ=255)

                                                 aaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                      >  1:839181/1‑93 (MQ=255)

                                                  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCGCCACGTCCGTAAGGACACTAACACCTGAAg                                      >  1:2950030/1‑93 (MQ=21)

                                                   tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  2:5810699/1‑93 (MQ=255)

                                                   tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  2:6837435/1‑93 (MQ=255)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  2:119587/1‑93 (MQ=21)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  1:6739157/1‑93 (MQ=21)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTAAACCCCCACGTCCGTAAGGACCCTAACCCCTGAAGCTa                                   >  1:4259514/1‑93 (MQ=21)

                                                      aGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                  >  1:5751096/1‑93 (MQ=21)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                 <  1:4659405/93‑1 (MQ=21)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                 >  1:3051779/1‑93 (MQ=21)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                               <  2:5902020/93‑1 (MQ=255)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGAAACTAACACCTGAAGCTAgcg                                >  1:2500975/1‑93 (MQ=21)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                >  1:681610/1‑93 (MQ=21)

                                                         aagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                               >  1:5651577/1‑93 (MQ=18)

                                                         aagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                               >  1:4866788/1‑93 (MQ=18)

                                                          agaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                              >  1:2148261/1‑93 (MQ=18)

                                                           gaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                             >  1:5362105/1‑93 (MQ=18)

                                                           gaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                            >  1:3533818/1‑93 (MQ=255)

                                                            aaaCAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                            <  2:2204511/93‑1 (MQ=18)

                                                              aCAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGGGTCTa                          >  1:440680/1‑93 (MQ=18)

                                                               cAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                          >  2:2785979/1‑93 (MQ=17)

                                                                 aGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                       >  1:212201/1‑93 (MQ=17)

                                                                  gAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                      >  1:6167119/1‑93 (MQ=17)

                                                                  gAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  1:1993203/1‑93 (MQ=255)

                                                                  gAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  2:4995339/1‑93 (MQ=255)

                                                                    aaGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  1:7517842/1‑93 (MQ=17)

                                                                    aaGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  1:3137153/1‑93 (MQ=17)

                                                                    aaGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                   >  2:3495870/1‑93 (MQ=255)

                                                                     aGGAAGAAAAGATAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                   <  2:1373798/93‑1 (MQ=14)

                                                                       gAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                 >  1:4068975/1‑93 (MQ=14)

                                                                        aaGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                >  2:290453/1‑93 (MQ=14)

                                                                        aaGTAAAGATAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                <  2:4350298/93‑1 (MQ=14)

                                                                         aGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc             >  1:3594608/1‑93 (MQ=255)

                                                                          gTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa              >  1:4805600/1‑93 (MQ=14)

                                                                          gTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc            >  2:3393670/1‑93 (MQ=255)

                                                                            aaaGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc            >  1:5454612/1‑93 (MQ=14)

                                                                             aaGATAATT‑‑GGTGG‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt           <  2:6196243/93‑1 (MQ=11)

                                                                             aaGATAATT‑‑GGTGG‑‑GA‑‑‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc         <  2:3735532/93‑1 (MQ=255)

                                                                             aaGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc         >  2:4440453/1‑93 (MQ=38)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  2:5937678/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  2:5689429/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  1:3405080/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  1:3214846/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  2:3965645/1‑93 (MQ=11)

                                                                               gATAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc         <  1:6996666/93‑1 (MQ=11)

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGT  >  NC_000913/4603563‑4603739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`