BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,356	0	A	G	74.4%	341.3 / 161.0	223	N311S (AAT→AGT)	yagF	putative dehydratase
Reads supporting (aligned to +/- strand): ref base A (33/24); new base G (96/70); total (129/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 225 total aligned reads displayed.

GGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGC  >  NC_000913/283264‑283448

   tAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGttt                                                                                           <  2:2220861/93‑1 (MQ=255)

    aGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTa                                                                                          <  2:4676864/93‑1 (MQ=255)

    aGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTa                                                                                          <  2:688252/93‑1 (MQ=255)

     gCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTAc                                                                                         >  2:7234961/1‑93 (MQ=255)

     gCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTAc                                                                                         >  2:4785101/1‑93 (MQ=255)

        gcgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTActgc                                                                                      >  2:6749401/1‑93 (MQ=255)

        gcgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTActgc                                                                                      >  1:3810274/1‑93 (MQ=255)

           gCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACtgctgc                                                                                   >  2:1796755/1‑93 (MQ=255)

           gCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACtgctgc                                                                                   >  2:1346593/1‑93 (MQ=255)

           gCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACtgctgc                                                                                   >  2:6181093/1‑93 (MQ=255)

             aTCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGcac                                                                                 >  2:1406524/1‑93 (MQ=255)

              tCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGcaca                                                                                <  1:4775330/93‑1 (MQ=255)

                accacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACAtt                                                                              >  1:5547177/1‑93 (MQ=255)

                accacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACAtt                                                                              <  2:7446173/93‑1 (MQ=255)

                accacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACAtt                                                                              >  2:6268892/1‑93 (MQ=255)

                accacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACAtt                                                                              >  1:102599/1‑93 (MQ=255)

                accacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACAtt                                                                              >  1:875088/1‑93 (MQ=255)

                 ccacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTc                                                                             <  1:5863833/93‑1 (MQ=255)

                 ccacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTc                                                                             <  1:4740549/93‑1 (MQ=255)

                 ccacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTc                                                                             >  1:1706749/1‑93 (MQ=255)

                 ccacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTc                                                                             >  1:5585012/1‑93 (MQ=255)

                  cacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTcc                                                                            <  2:5783054/93‑1 (MQ=255)

                  cacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTcc                                                                            <  2:5183424/93‑1 (MQ=255)

                   acGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCg                                                                           <  1:5877446/93‑1 (MQ=255)

                   acGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCg                                                                           <  2:4536608/93‑1 (MQ=255)

                       gggATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCa                                                                       >  2:6141557/1‑93 (MQ=255)

                       gggATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCa                                                                       >  1:2008051/1‑93 (MQ=255)

                        ggATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCAt                                                                      >  2:6382623/1‑93 (MQ=255)

                         gATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATc                                                                     <  1:2576031/93‑1 (MQ=255)

                         gATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATc                                                                     <  1:1821662/93‑1 (MQ=255)

                          atatCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCg                                                                    >  2:2025371/1‑93 (MQ=255)

                           tatCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGc                                                                   >  1:6464539/1‑93 (MQ=255)

                           tatCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGc                                                                   <  2:5814787/93‑1 (MQ=255)

                            atCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGcc                                                                  >  2:2987909/1‑93 (MQ=255)

                             tCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGccc                                                                 >  1:2347767/1‑93 (MQ=255)

                                tctcCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCAcg                                                              >  1:5007603/1‑93 (MQ=255)

                                tctcCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCAcg                                                              <  2:5981347/93‑1 (MQ=255)

                                tctcCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCAcg                                                              >  2:4775238/1‑93 (MQ=255)

                                 ctcCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCAcgc                                                             >  2:2854803/1‑93 (MQ=255)

                                 ctcCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCAcgc                                                             >  1:2507832/1‑93 (MQ=255)

                                  tcCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCAcgcg                                                            >  1:6722755/1‑93 (MQ=255)

                                    cGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACgcggc                                                          >  1:2119563/1‑93 (MQ=255)

                                     gATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACgcggcg                                                         >  2:4959185/1‑93 (MQ=255)

                                     gATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACgcggcg                                                         >  2:698420/1‑93 (MQ=255)

                                     gATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACgcggcg                                                         <  1:6932531/93‑1 (MQ=255)

                                     gATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGCCCATCGTCCACgcggcg                                                         >  1:3610664/1‑93 (MQ=255)

                                      aTAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGcggcgg                                                        >  1:2200036/1‑93 (MQ=255)

                                        aaaGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGc                                                      <  2:6048458/93‑1 (MQ=255)

                                         aaGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCt                                                     >  1:1923658/1‑93 (MQ=255)

                                          aGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTg                                                    <  1:4563613/93‑1 (MQ=255)

                                           gCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGc                                                   >  1:1108662/1‑93 (MQ=255)

                                            ccATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCa                                                  >  2:3398171/1‑93 (MQ=255)

                                             cATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCAc                                                 <  1:6196329/93‑1 (MQ=255)

                                              aTCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACg                                                >  2:2879717/1‑93 (MQ=255)

                                               tCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGa                                               >  2:1020073/1‑93 (MQ=255)

                                               tCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGa                                               <  1:2680737/93‑1 (MQ=255)

                                                cGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGAt                                              <  1:4485831/93‑1 (MQ=255)

                                                cGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGAt                                              <  1:2840823/93‑1 (MQ=255)

                                                 gAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATc                                             <  1:1533478/93‑1 (MQ=255)

                                                 gAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATc                                             >  2:4318309/1‑93 (MQ=255)

                                                 gAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATc                                             >  2:6859188/1‑93 (MQ=255)

                                                 gAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATc                                             <  1:1590033/93‑1 (MQ=255)

                                                   aaaCGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATccc                                           <  1:59181/93‑1 (MQ=255)

                                                      cgcgATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGa                                        <  1:4549507/93‑1 (MQ=255)

                                                      cgcgATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGa                                        <  1:7463550/93‑1 (MQ=255)

                                                       gcgATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGAc                                       >  1:1609734/1‑93 (MQ=255)

                                                        cgATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACg                                      <  2:3451362/93‑1 (MQ=255)

                                                         gATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGt                                     <  2:2390937/93‑1 (MQ=255)

                                                         gATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGt                                     <  2:2466900/93‑1 (MQ=255)

                                                         gATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGt                                     >  2:3006243/1‑93 (MQ=255)

                                                           tGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTg                                   >  1:4137849/1‑93 (MQ=255)

                                                           tGGTGATCCACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTg                                   >  2:4045395/1‑93 (MQ=255)

                                                                 tCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACt                             >  1:4102366/1‑93 (MQ=255)

                                                                 tCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACt                             >  1:272037/1‑93 (MQ=255)

                                                                 tCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACt                             >  1:6807366/1‑93 (MQ=255)

                                                                   cACGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTgg                           >  1:3035031/1‑93 (MQ=255)

                                                                    aCGCGGCGTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGa                          <  2:5410073/93‑1 (MQ=255)

                                                                      gcggcgTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAcg                        >  2:3707993/1‑93 (MQ=255)

                                                                        ggcgTTCGGCGGCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAcgcg                      >  1:1288279/1‑93 (MQ=255)

                                                                           gTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCAt                   <  2:5563928/93‑1 (MQ=255)

                                                                              cggcggCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCaa                <  2:3302443/93‑1 (MQ=255)

                                                                              cggcggCTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCaa                >  1:4755709/1‑93 (MQ=255)

                                                                               ggcggcTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAc               <  2:7416718/93‑1 (MQ=255)

                                                                               ggcggcTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAc               >  1:5961542/1‑93 (MQ=255)

                                                                                gcggcTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAcc              >  1:92726/1‑93 (MQ=255)

                                                                                  ggcTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGt            >  2:414663/1‑93 (MQ=255)

                                                                                  ggcTCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGt            >  1:1775765/1‑93 (MQ=255)

                                                                                    cTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTaa          <  1:768401/93‑1 (MQ=255)

                                                                                     tCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTaaa         <  1:3244640/93‑1 (MQ=255)

                                                                                     tCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTaaa         >  2:1659167/1‑93 (MQ=255)

                                                                                     tCCACCAGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTaaa         >  1:574289/1‑93 (MQ=255)

                                                                                      ccaccaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAg        >  1:2217564/1‑93 (MQ=255)

                                                                                       caccaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGt       >  1:4067221/1‑93 (MQ=255)

                                                                                        accaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg      >  1:3678238/1‑93 (MQ=255)

                                                                                        accaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg      >  1:4656870/1‑93 (MQ=255)

                                                                                        accaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg      >  1:7023426/1‑93 (MQ=255)

                                                                                        accaATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg      >  2:86714/1‑93 (MQ=255)

                                                                                         ccaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGc     >  2:3528421/1‑93 (MQ=255)

                                                                                         ccaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGc     >  1:7397322/1‑93 (MQ=255)

                                                                                         ccaGTTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGc     <  1:1132568/93‑1 (MQ=255)

GGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGC  >  NC_000913/283264‑283448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`