BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	578,027	T→C	A30A (GCA→GCG)	ybcU ←	Bor‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	578,027	0	T	C	86.5%	505.2 / 70.0	208	A30A (GCA→GCG)	ybcU	Bor‑like protein
Reads supporting (aligned to +/- strand): ref base T (9/19); new base C (59/121); total (68/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 215 total aligned reads displayed.

GCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCAT  >  NC_000913/577937‑578116

gccgccACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTAccgc                                                                                         <  1:5438918/93‑1 (MQ=255)

 ccgccACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTAccgct                                                                                        <  1:5497031/93‑1 (MQ=255)

   gccACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGctgt                                                                                      <  2:2565232/93‑1 (MQ=255)

    ccACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGctgtc                                                                                     <  1:111637/93‑1 (MQ=255)

     cacaAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGctgtct                                                                                    >  1:986244/1‑93 (MQ=255)

      acaAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGctgtctg                                                                                   >  2:2500426/1‑93 (MQ=255)

      acaAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGctgtctg                                                                                   >  2:2398639/1‑93 (MQ=255)

       caAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGctgtctgt                                                                                  >  2:2425111/1‑93 (MQ=255)

       caAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGctgtctgt                                                                                  >  1:662568/1‑93 (MQ=255)

        aaaTTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCtgtt                                                                                 <  2:742246/93‑1 (MQ=255)

        aaaTTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCtgtt                                                                                 <  1:2758906/93‑1 (MQ=255)

         aaTTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCtgttt                                                                                <  1:309848/93‑1 (MQ=255)

          aTTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCtgtttg                                                                               <  2:6816138/93‑1 (MQ=255)

           ttttAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCtgtttgt                                                                              >  1:6255397/1‑93 (MQ=255)

           ttttAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCtgtttgt                                                                              >  2:3368118/1‑93 (MQ=255)

            tttAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCtgtttgtt                                                                             <  2:7130715/93‑1 (MQ=255)

             ttAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTgtttgttt                                                                            <  2:5834009/93‑1 (MQ=255)

              tAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGtttt                                                                           <  2:4347873/93‑1 (MQ=255)

                 cTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGaa                                                                        <  2:2628463/93‑1 (MQ=255)

                  tGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAAc                                                                       <  1:4010168/93‑1 (MQ=255)

                   gCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACa                                                                      <  2:4669047/93‑1 (MQ=255)

                   gCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACa                                                                      <  1:3045035/93‑1 (MQ=255)

                   gCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACa                                                                      <  2:5135282/93‑1 (MQ=255)

                   gCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACa                                                                      >  2:6089275/1‑93 (MQ=255)

                     aTCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGt                                                                    <  1:1813942/93‑1 (MQ=255)

                      tCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTa                                                                   <  1:6851190/93‑1 (MQ=255)

                      tCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTa                                                                   >  1:598497/1‑93 (MQ=255)

                        gACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTaaa                                                                 <  2:2028942/93‑1 (MQ=255)

                        gACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTaaa                                                                 <  2:1928627/93‑1 (MQ=255)

                            gTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTc                                                             <  1:4652147/93‑1 (MQ=255)

                             ttttCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCt                                                            >  1:7155564/1‑93 (MQ=255)

                             ttttCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCt                                                            <  1:2556427/93‑1 (MQ=255)

                              tttCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTg                                                           <  1:7173088/93‑1 (MQ=255)

                              tttCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTg                                                           <  2:3801715/93‑1 (MQ=255)

                               ttcttcTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGt                                                          <  2:5192733/93‑1 (MQ=255)

                                tcttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGtt                                                         <  1:915885/93‑1 (MQ=255)

                                tcttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGtt                                                         <  2:5257533/93‑1 (MQ=255)

                                tcttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGtt                                                         <  2:7226419/93‑1 (MQ=255)

                                 cttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTg                                                        <  1:5354021/93‑1 (MQ=255)

                                  ttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGa                                                       <  2:5674415/93‑1 (MQ=255)

                                  ttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGa                                                       <  2:6723928/93‑1 (MQ=255)

                                    ctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGc                                                     <  2:4433711/93‑1 (MQ=255)

                                    ctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGc                                                     >  2:5938403/1‑93 (MQ=255)

                                     tGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGca                                                    >  2:5216055/1‑93 (MQ=255)

                                      gCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGcac                                                   <  2:3167254/93‑1 (MQ=255)

                                      gCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGcac                                                   >  2:6577979/1‑93 (MQ=255)

                                      gCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGcac                                                   <  2:811427/93‑1 (MQ=255)

                                       cccAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGcaca                                                  <  2:6624097/93‑1 (MQ=255)

                                       cccAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGcaca                                                  <  2:3647562/93‑1 (MQ=255)

                                       cccAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGcaca                                                  <  2:3499408/93‑1 (MQ=255)

                                        ccAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACAt                                                 >  1:2676011/1‑93 (MQ=255)

                                        ccAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACAt                                                 >  2:6271317/1‑93 (MQ=255)

                                         cAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATc                                                >  2:2087246/1‑93 (MQ=255)

                                          aaTTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATcc                                               >  1:2157580/1‑93 (MQ=255)

                                          aaTTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATcc                                               >  2:3538533/1‑93 (MQ=255)

                                          aaTTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATcc                                               >  1:5747447/1‑93 (MQ=255)

                                            ttCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTg                                             <  1:2963216/93‑1 (MQ=255)

                                            ttCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTg                                             <  1:5098790/93‑1 (MQ=255)

                                            ttCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTg                                             <  1:1472957/93‑1 (MQ=255)

                                             tCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGt                                            <  1:1960139/93‑1 (MQ=255)

                                              ccAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGta                                           <  2:6507073/93‑1 (MQ=255)

                                              ccAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGATTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGta                                           <  1:6217703/93‑1 (MQ=255)

                                               cAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGtaa                                          <  2:7064517/93‑1 (MQ=255)

                                               cAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGtaa                                          <  2:4284311/93‑1 (MQ=255)

                                                aGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGtaat                                         >  1:2401823/1‑93 (MQ=255)

                                                 gAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGtaata                                        >  1:5399977/1‑93 (MQ=255)

                                                  aaaCGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGtaataa                                       <  1:5854023/93‑1 (MQ=255)

                                                    aCGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGc                                     <  1:1396121/93‑1 (MQ=255)

                                                    aCGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGc                                     >  1:1702613/1‑93 (MQ=255)

                                                    aCGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGc                                     <  1:5245043/93‑1 (MQ=255)

                                                      gaagaaATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAg                                   <  2:5338360/93‑1 (MQ=255)

                                                      gaagaaATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAg                                   <  1:1650835/93‑1 (MQ=255)

                                                       aagaaATGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAgg                                  >  1:52833/1‑93 (MQ=255)

                                                          aaaTGATGGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGcc                               <  2:6351854/93‑1 (MQ=255)

                                                            atgatgGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAg                             >  2:7372056/1‑93 (MQ=255)

                                                             tgatgGGTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAgc                            >  2:3983430/1‑93 (MQ=255)

                                                              gatgGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAgcg                           >  1:3894883/1‑93 (MQ=255)

                                                                   gTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTa                      <  1:2086934/93‑1 (MQ=255)

                                                                   gTGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTa                      <  1:3191433/93‑1 (MQ=255)

                                                                    tGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAg                     >  1:374007/1‑93 (MQ=255)

                                                                    tGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAg                     >  2:1861423/1‑93 (MQ=255)

                                                                    tGATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAg                     <  1:4167426/93‑1 (MQ=255)

                                                                     gATGGTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGc                    >  1:3373954/1‑93 (MQ=255)

                                                                      aTGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCg                   <  2:2238686/93‑1 (MQ=255)

                                                                        ggTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAg                 <  1:1311044/93‑1 (MQ=255)

                                                                        ggTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAg                 <  2:2040574/93‑1 (MQ=255)

                                                                         gTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGt                <  2:4259747/93‑1 (MQ=255)

                                                                         gTTTCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGt                <  1:6732141/93‑1 (MQ=255)

                                                                          tttCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTa               >  2:6458095/1‑93 (MQ=255)

                                                                          tttCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTa               >  1:1974188/1‑93 (MQ=255)

                                                                          tttCCTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTa               <  2:3095569/93‑1 (MQ=255)

                                                                             ccTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCa            <  2:4017874/93‑1 (MQ=255)

                                                                              cTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAt           >  2:5637693/1‑93 (MQ=255)

                                                                              cTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAt           <  1:4458690/93‑1 (MQ=255)

                                                                              cTTTGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAt           <  1:734346/93‑1 (MQ=255)

                                                                               tttGGTGCTACTACTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAtt          >  2:1437563/1‑93 (MQ=255)

                                                                                ttGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAttt         <  1:4400620/93‑1 (MQ=255)

                                                                                ttGGTGCTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAttt         >  1:6066524/1‑93 (MQ=255)

                                                                                      cTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCa   <  2:6199798/93‑1 (MQ=255)

                                                                                      cTACCGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCa   <  2:5014266/93‑1 (MQ=255)

GCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCAT  >  NC_000913/577937‑578116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`