BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	582,225	A→G	Q43R (CAG→CGG)	ylcE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	582,225	0	A	G	84.0%	574.8 / 108.1	257	Q43R (CAG→CGG)	ylcE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (22/19); new base G (115/101); total (137/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 260 total aligned reads displayed.

TATGCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCGTGTGGACA  >  NC_000913/582134‑582317

tATGCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAg                                                                                             <  2:2815795/93‑1 (MQ=255)

  tGCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGa                                                                                           >  2:5938807/1‑93 (MQ=255)

  tGCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGa                                                                                           <  1:4124710/93‑1 (MQ=255)

  tGCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGa                                                                                           >  1:4305905/1‑93 (MQ=255)

   gCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGAt                                                                                          <  2:7269939/93‑1 (MQ=255)

   gCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGAt                                                                                          >  2:4354740/1‑93 (MQ=255)

   gCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGAt                                                                                          >  1:7327382/1‑93 (MQ=255)

     ttCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGc                                                                                        >  1:1633921/1‑93 (MQ=255)

     ttCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGc                                                                                        >  1:5904875/1‑93 (MQ=255)

        aaTACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGt                                                                                     >  2:546090/1‑93 (MQ=255)

         aTACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTa                                                                                    <  2:696024/93‑1 (MQ=255)

         aTACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTa                                                                                    <  2:1916612/93‑1 (MQ=255)

         aTACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTa                                                                                    >  1:5400758/1‑93 (MQ=255)

         aTACAGCAGGAGCCGAATGGCTGGAAGACCGTCAACACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTa                                                                                    >  2:1501721/1‑93 (MQ=255)

          tACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAg                                                                                   >  1:2494961/1‑93 (MQ=255)

          tACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAg                                                                                   >  2:3988556/1‑93 (MQ=255)

          tACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAg                                                                                   >  2:646060/1‑93 (MQ=255)

           aCAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGa                                                                                  <  2:5067675/93‑1 (MQ=255)

             agcagGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATc                                                                                <  2:2361303/93‑1 (MQ=255)

              gcagGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCt                                                                               >  2:6391300/1‑93 (MQ=255)

              gcagGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCt                                                                               >  2:2607269/1‑93 (MQ=255)

               cagGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTg                                                                              <  2:7441695/93‑1 (MQ=255)

               cagGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTg                                                                              <  1:805987/93‑1 (MQ=255)

                agGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTgg                                                                             <  1:5729929/93‑1 (MQ=255)

                 ggAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGa                                                                            <  1:6863023/93‑1 (MQ=255)

                 ggAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGa                                                                            <  1:496132/93‑1 (MQ=255)

                    gCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAAt                                                                         <  2:2007159/93‑1 (MQ=255)

                    gCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAAt                                                                         >  2:469184/1‑93 (MQ=255)

                    gCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAAt                                                                         <  1:5869393/93‑1 (MQ=255)

                    gCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAAt                                                                         <  2:1682189/93‑1 (MQ=255)

                      cGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCg                                                                       >  2:3379857/1‑93 (MQ=255)

                       gAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGc                                                                      >  2:7099927/1‑93 (MQ=255)

                       gAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGc                                                                      >  1:2514388/1‑93 (MQ=255)

                        aaTGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCa                                                                     >  1:3609019/1‑93 (MQ=255)

                         aTGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCaa                                                                    <  2:1029562/93‑1 (MQ=255)

                          tGGCTGGAAGACCGTCACCACACAGTTTTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGAAATCGCAAc                                                                   >  1:4476289/1‑93 (MQ=255)

                           ggCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACg                                                                  >  1:4411576/1‑93 (MQ=255)

                            gCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACgg                                                                 >  1:4842980/1‑93 (MQ=255)

                             cTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGAAATCGCAACgga                                                                <  2:6199347/93‑1 (MQ=255)

                              tGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGAAATCGCAACggag                                                               >  1:2670999/1‑93 (MQ=255)

                               ggAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACggagg                                                              >  1:6833065/1‑93 (MQ=255)

                               ggAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACggagg                                                              >  1:4261929/1‑93 (MQ=255)

                               ggAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACggagg                                                              >  1:6543041/1‑93 (MQ=255)

                                gAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACggagga                                                             <  2:1564125/93‑1 (MQ=255)

                                  aGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGgaag                                                           >  1:277011/1‑93 (MQ=255)

                                   gACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGgaaga                                                          >  2:6984498/1‑93 (MQ=255)

                                   gACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGgaaga                                                          >  1:7474007/1‑93 (MQ=255)

                                       gTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGaagaaaga                                                      >  2:272393/1‑93 (MQ=255)

                                        tCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGAt                                                     >  1:3472414/1‑93 (MQ=255)

                                         caccacACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATc                                                    >  2:3908751/1‑93 (MQ=255)

                                          accacACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCg                                                   <  1:6720485/93‑1 (MQ=255)

                                          accacACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCg                                                   >  1:3523265/1‑93 (MQ=255)

                                          accacACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCg                                                   >  2:5548111/1‑93 (MQ=255)

                                           ccacACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGt                                                  <  2:3245354/93‑1 (MQ=255)

                                           ccacACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGt                                                  <  2:1819757/93‑1 (MQ=255)

                                           ccacACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGt                                                  >  1:3772554/1‑93 (MQ=255)

                                            cacacaGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGtt                                                 >  1:947068/1‑93 (MQ=255)

                                            cacacaGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGtt                                                 <  1:6082678/93‑1 (MQ=255)

                                            cacacaGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGtt                                                 <  2:7386300/93‑1 (MQ=255)

                                             acacaGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTg                                                <  1:2010172/93‑1 (MQ=255)

                                              cacaGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGc                                               <  1:1167828/93‑1 (MQ=255)

                                                caGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTg                                             >  2:2303850/1‑93 (MQ=255)

                                                  gTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGa                                           >  2:7424837/1‑93 (MQ=255)

                                                   ttCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGaa                                          >  2:4179685/1‑93 (MQ=255)

                                                      tACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGcc                                       >  2:2147539/1‑93 (MQ=255)

                                                      tACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGcc                                       >  2:6710101/1‑93 (MQ=255)

                                                       aCATCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCt                                      <  2:6314200/93‑1 (MQ=255)

                                                         aTCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGa                                    >  2:4746243/1‑93 (MQ=255)

                                                         aTCCAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGa                                    >  2:1249418/1‑93 (MQ=255)

                                                           ccAGGAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGaaa                                  >  1:1507261/1‑93 (MQ=255)

                                                              ggAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGaaaaaa                               >  2:7341284/1‑93 (MQ=255)

                                                              ggAATTCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGaaaaaa                               <  1:2710514/93‑1 (MQ=255)

                                                                   tCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATc                          <  2:6544581/93‑1 (MQ=255)

                                                                   tCAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATc                          <  1:318097/93‑1 (MQ=255)

                                                                    cAGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCg                         >  2:1366881/1‑93 (MQ=255)

                                                                     aGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCgg                        <  1:3820034/93‑1 (MQ=255)

                                                                     aGTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCgg                        <  1:1467852/93‑1 (MQ=255)

                                                                      gTGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCggg                       >  1:2100834/1‑93 (MQ=255)

                                                                       tGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGt                      >  2:775404/1‑93 (MQ=255)

                                                                       tGAGTATATTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGt                      >  2:3730174/1‑93 (MQ=255)

                                                                         aGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGtgc                    >  2:5292035/1‑93 (MQ=255)

                                                                            atatTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGtgctgc                 <  1:1239911/93‑1 (MQ=255)

                                                                            atatTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGtgctgc                 <  1:2229552/93‑1 (MQ=255)

                                                                            atatTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGtgctgc                 <  2:139691/93‑1 (MQ=255)

                                                                             tatTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTgctgct                >  1:6122770/1‑93 (MQ=255)

                                                                              atTGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTa               >  2:4511327/1‑93 (MQ=255)

                                                                               ttGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTaa              <  2:3635455/93‑1 (MQ=255)

                                                                               ttGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTaa              <  1:207800/93‑1 (MQ=255)

                                                                                tGCGCCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTaaa             <  1:5182296/93‑1 (MQ=255)

                                                                                 gcgcCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAAc            <  2:29670/93‑1 (MQ=255)

                                                                                 gcgcCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAAc            <  1:1822082/93‑1 (MQ=255)

                                                                                  cgcCACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAAcc           <  1:3695254/93‑1 (MQ=255)

                                                                                     cACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCgtg        >  2:5658282/1‑93 (MQ=255)

                                                                                     cACTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCgtg        >  1:3932456/1‑93 (MQ=255)

                                                                                      aCTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAAGCgtgg       <  1:4873935/93‑2 (MQ=255)

                                                                                       cTGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCGtgtg      >  1:3091215/1‑93 (MQ=255)

                                                                                        tGCGGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCGTGTgg     >  1:6499182/1‑93 (MQ=255)

                                                                                         gCAGGATGCTGTAGATCTGGAAATCACAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCGTGGGGa    <  1:6975499/93‑1 (MQ=255)

                                                                                         gCAGGATGCTGTAGATCTGGAAATAGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCGTGTGGa    <  2:2464577/93‑1 (MQ=255)

                                                                                           aGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCGTGTGGaca  >  1:7413038/1‑93 (MQ=255)

TATGCTTCAATACAGCAGGAGCCGAATGGCTGGAAGACCGTCACCACACAGTTCTACATCCAGGAATTCAGTGAGTATATTGCGCCACTGCAGGATGCTGTAGATCTGGAAATCGCAACGGAGGAAGAAAGATCGTTGCTGGAAGCCTGAAAAAAGTATCGGGTGCTGCTAAACCGTGTGGACA  >  NC_000913/582134‑582317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`